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pEF-FLAG-mouse Munc18-1 (#RDB15177)

Expression vector of mouse Munc18-1 [Stxbp1 in NCBI].

Clone info. Expression vector of mouse Munc18-1 [Stxbp1 in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Stxbp1 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. J. Biol. Chem. 278 (17): 15390-15396, 2003)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 278 (17): 15390-15396, 2003)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15177 pEF-FLAG-mouse Munc18-1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse Munc18-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15177).

Reference section:

Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15177_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1alpha pro, FLAG, insert 5'
Sequence file: RDB15177_A7Fma.seq check
>D04709B3_2_A7Fm_EF1a-C_F_D03_12_ABI24.ab1
    1 CCTGGGGAAT TGCCCTTTTT GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAGGAAT TCTCTAGAGC GGCCGCGGGA
  121 ATTCGATTCC ACCATGGACT ACAAGGACGA TGACGACAAG GGATCCATGG CCCCCATTGG
  181 CCTCAAGGCG GTGGTCGGAG AGAAGATCAT GCATGATGTG ATCAAGAAGG TGAAGAAGAA
  241 GGGCGAGTGG AAGGTGCTGG TGGTGGACCA GTTAAGCATG AGGATGCTGT CCTCCTGCTG
  301 CAAGATGACA GACATCATGA CCGAGGGCAT CACAATTGTG GAGGATATCA ACAAGCGCCG
  361 AGAGCCGCTC CCCAGCCTGG AGGCCGTGTA CCTCATCACC CCATCTGAGA AGTCTGTCCA
  421 CTCTCTGATC AGTGATTTTA AGGACCCGCC GACTGCTAAA TATCGGGCTG CGCATGTGTT
  481 CTTCACAGAC TCGTGTCCAG ATGCCCTATT TAACGAGCTG GTAAAATCCC GAGCAGCCAA
  541 AGTCATCAAG ACGCTGACGG AAATCAACAT TGCGTTTCTC CCCTATGAGT CCCAGGTGTA
  601 TTCCCTGGAC TCCGCTGACT CTTTCCAAAG CTTCTACAGC CCTCACAAGG CGCAGATGAA
  661 GAATCCGATA CTGGAACGCC TGGCAGAGCA GATCGCAACC CTGTGTGCCA CCCTGAAGGA
  721 GTATCCAGCT GTGCGGTATC GGGGGGAGTA CAAGGACAAT GCCTTGCTGG CTCAGCTGAT
  781 CCAGGACAAG CTGGATGCCT ATAAAGCCGA CGATCCAACA ATGGGGGAGG GTCCGACAAG
  841 GCACGGTCCC AGCTCCTGAT CCTGGATCGT GGCTTTGACC CCAGCTCCCC TGTGCTCCAT
  901 GAACTGACAT TCCAGGCTAT GAGTTACGAC CTGCTGCCTA TCGAAAATGA TGTTTACAAG
  961 TATGAGACCA GCGGCATTGG AGAGGCGCGG TGAAGGAGGT GCTACTGGAT GAGGACGATG
 1021 ACCTGTGGAT TGCGCTGCGA CAACAAGCAC ATCGCAGAGG TGTCCCAGGA GTCCACCCGG
 1081 GTCTCTGACG AACTTTTCTC TAGCAGAGGA ATGAACACTG CCGGCAGGAC ACATTGCGGT
1141 ACTGATCCCA GATGCCTGGA AGAAAAGTGT CTCA
//
Primer: hGCSF-R (Pr0031)
Region: hGCSF+p(A), insert 3'
Sequence file: RDB15177_A7Fmb.seq check
>D04709B3_2_A7Fm_hGCSF-R_E03_15_ABI24.ab1
    1 GTGGGGACTG ATCTCTCAGG GTCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT
   61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTTAACT GCTTATTTCT TCATCTGTTT
  121 TATTCAGCTT CTTCAGCGTG TCCAGCAGTT TCTGTGGGGT GAGAATGTGC GTGGATCCTA
  181 TCAGCACTTC CCACTTGCCG TTGGCCTGGG TCACTTCGTA AGCACAGCGC ATCTCATTCA
  241 GGCTCACACC CCCAAGGATG AAAATAATGA GGCGGGGACC GCTGCGGTAC TCCCCGGGGG
  301 CCTTATTCTT GTGCCAATGT CCATAGCGGG CACTCACAGC AGTGGTGCTG AAGGACGCGG
  361 ACGAGCGGGT AGAGATGTAT GGGTAGTGCT TTGTATCCAG CTTGTCTTCG ATAGTGTCCT
  421 CCATAATGTC TTTAATGATC GGGGTCCATC GTGAGAGCTG GTAGGTCTGC TCACTGATAC
  481 GCTCCTTCCG CTCCGGTTTG CTTCGGCGGC GTAGTGTGGA ATCCGTGACG ATGGGCACGC
  541 CGAGGTGAGC CATGTTGGTG ATGATCTCGC TGTCCTCTGG GGGTATCTGA GCGTGCTGGA
  601 TGAGTTTGTT TAGGTTCTCC TCAGTGATAC CGTTCTTCAG GAAGATGTAG AGAAGGATGA
  661 TACGGATTTT GTCGTAAGTG CTGACGTTCG CATCCAGCAG GATGGGGACA ATGGCTCTCA
  721 TGGGGTCCTT GATTTTTTCC CCCTCAGCAT CTGTGCCCAT TGCCAGGTCC TGCTCCACGC
  781 GGCAGAGTTT GTCTACAGTG CCTTGGTAAT GCTTCATGCA GTCTTCAGCA AGGTGCAGGT
  841 GAGTCGAATA CTTGCTGAGC TCCTTCTGGT ACTGGGGCAT TTTCTTCAGC ATCTGGGACA
  901 GGTCCCGCAT GGTGGTCTTC TCGCCAGTGT TCATCCTCTT GCTAGAGGAA AAGTCCTTCA
  961 GAGACCGGGT GACTTCCTGG GACACCTCTG CGATGTGCTT GTGTCGCAGC GCAATCCACA
 1021 GGTCATCGTC CTCATCCAGT AGCACCTCCC TTCACCCGCG CCTCTCAATG CCGCTGGTCT
 1081 CATACTTTGT AAACATCATT TTTCGGATAG GCAGCAGTTC GTACTTCATA GCTGGATGGT
1141 CAGTTCATGG AAGCCACAGG GGAAGCTGGG GGTTCCAAAG GTTCAAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134. [link to RRC of NBRP]