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Expression vector of mouse synaptosomal-associated protein 25 (SNAP-25).
| Catalog number | RDB15176 |
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| Resource name | pEF-FLAG-mouse SNAP-25 |
| Clone info. | Expression vector of mouse synaptosomal-associated protein 25 (SNAP-25), tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 214 to 834nt of NM_011428.3 (cds, 214 to 834nt) is cloned. Expression was confirmed by the depositor with immunoblotting. Known difference: A to G substitution at 222nt of NM__011428.3 (synonym). |
| Vector backbone | pEF-BOS FLAG (Plasmid) |
| Size of vector backbone | 5.7 kb |
| Selectable markers | Amp^r |
| Gene/insert name | mouse Snap25 cDNA |
| Depositor|Developer | Fukuda, Mitsunori | |
| Other clones in our bank | mouse Snap25 (NCBI Gene 20614) | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002) |
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| Additional terms and conditions for distribution | Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
| Ordering | Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment ] [Bank Transfer or Check Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB15176 | pEF-FLAG-mouse SNAP-25 | DNA solution |
icon below as well as clone information before placing your order.Electronic file
| Electronic file | Nucleotide sequence (partial) provided by the depositor RDB15176pka01.seq |
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Original reference
Further references such as user reports and related articles (go to bottom)
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB15176_A7Fmp1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: EF1a-C_F Region: EF-1 alpha pro, FLAG, insert, hGCSF+p(A) Sequence file: RDB15176_A7Fma.seq
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>D04709B2_1_A7Fm_EF1a-C_F_B03_06_ABI24.ab1 1 TCAGCATGCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA 61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG 121 ATTCCACCAT GGACTACAAG GACGATGACG ACAAGGGATC CATGGCCGAG GACGCAGACA 181 TGCGCAATGA GCTGGAGGAG ATGCAGAGGA GGGCTGACCA GCTGGCTGAT GAGTCCCTGG 241 AAAGCACCCG TCGCATGCTG CAGCTGGTTG AAGAGAGTAA AGATGCTGGC ATCAGGACTT 301 TGGTTATGTT GGATGAGCAA GGCGAACAAC TGGAACGCAT TGAGGAAGGG ATGGACCAAA 361 TCAATAAGGA CATGAAAGAA GCAGAAAAGA ATTTGACGGA CCTAGGAAAA TTCTGCGGGC 421 TTTGTGTGTG TCCCTGTAAC AAGCTTAAAT CCAGTGATGC TTACAAAAAA GCCTGGGGCA 481 ATAATCAGGA TGGAGTAGTG GCCAGCCAGC CTGCCCGTGT GGTGGATGAA CGGGAGCAGA 541 TGGCCATCAG TGGTGGCTTC ATCCGCAGGG TAACAAATGA TGCCCGGGAA AATGAGATGG 601 ATGAGAACCT GGAGCAGGTG AGCGGCATCA TCGGAAACCT CCGCCATATG GCTCTAGACA 661 TGGGCAATGA GATTGACACC CAGAATCGCC AGATCGACAG GATCATGGAG AAGGCTGATT 721 CCAACAAAAC CAGAATTGAT GAAGCCAACC AACGTGCAAC AAAGATGCTG GGAAGTGGTT 781 AATCACTAGT GAATTCGCGG CCGCTCTAGA GTGAGGGTCC CCACCTGGGA CCCTTGAGAG 841 TATCAGTCTC CCACGTGGGA GACAAGAAAT CCCTGTTTAA TATTTAAACA GCAGTGTTCC 901 CCATCTGGGT CCTTGCACCC CTCACTCTGG CCTCAGCCGA CTGCACAGCG GCCCCTGCAT 961 CCCCTTGGCT GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT 1021 GGGGTCCCAC GATTTGCTGG GGATCTCGTT TTTCTTCTAG ACTTTTGGGA CATGATTTGA 1081 CTCCCGAACA TCAACGAACG TGTCTCTGCT TTTTCTGGAT GCTCGAACGC TGGTCTGGCC 1141 CCACGAGGTC AGACTGTGAC TCTTTTTTTA AGGGG // |
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Primer: hGCSF-R Region: hGCSF+p(A), insert, FLAG, EF-1 alpha pro Sequence file: RDB15176_A7Fmb.seq
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>D04709B2_1_A7Fm_hGCSF-R_C03_09_ABI24.ab1 1 TGGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGAATT 61 CACTAGTGAT TAACCACTTC CCAGCATCTT TGTTGCACGT TGGTTGGCTT CATCAATTCT 121 GGTTTTGTTG GAATCAGCCT TCTCCATGAT CCTGTCGATC TGGCGATTCT GGGTGTCAAT 181 CTCATTGCCC ATGTCTAGAG CCATATGGCG GAGGTTTCCG ATGATGCCGC TCACCTGCTC 241 CAGGTTCTCA TCCATCTCAT TTTCCCGGGC ATCATTTGTT ACCCTGCGGA TGAAGCCACC 301 ACTGATGGCC ATCTGCTCCC GTTCATCCAC CACACGGGCA GGCTGGCTGG CCACTACTCC 361 ATCCTGATTA TTGCCCCAGG CTTTTTTGTA AGCATCACTG GATTTAAGCT TGTTACAGGG 421 ACACACACAA AGCCCGCAGA ATTTTCCTAG GTCCGTCAAA TTCTTTTCTG CTTCTTTCAT 481 GTCCTTATTG ATTTGGTCCA TCCCTTCCTC AATGCGTTCC AGTTGTTCGC CTTGCTCATC 541 CAACATAACC AAAGTCCTGA TGCCAGCATC TTTACTCTCT TCAACCAGCT GCAGCATGCG 601 ACGGGTGCTT TCCAGGGACT CATCAGCCAG CTGGTCAGCC CTCCTCTGCA TCTCCTCCAG 661 CTCATTGCGC ATGTCTGCGT CCTCGGCCAT GGATCCCTTG TCGTCATCGT CCTTGTAGTC 721 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA 781 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA 841 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC 901 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA 961 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA 1021 CATGAAGCGA CGGCTGAGGA CGGGAAAGGC CCTTTTCCTT TTGTGTGGGT GACTCACCGG 1081 CCCGCTCTCC CGAGCGCCGC GTCCTCCATT TTGAGCCTCC CTGCAGCCAG GGACGGAGCG 1141 GTCAATCTTT CCGCTCACGC AACTGGGTGG CCCGGACCCG GGGGCCAGAG CCTC // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2021.05.03
GNP_filter3_RDBDEP_html_210401.pl