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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse SNAP-25 (#RDB15176)

Expression vector of mouse synaptosomal-associated protein 25 (SNAP-25).

Clone info. Expression vector of mouse synaptosomal-associated protein 25 (SNAP-25), tagged with N-terminal FLAG, EF1 promoter. cDNA fragment 214 to 834nt of NM_011428.3 (cds, 214 to 834nt) is cloned.
Comment Expression was confirmed by the depositor with immunoblotting. Known difference: A to G substitution at 222nt of NM__011428.3 (synonym).
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Snap25 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15176pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15176 pEF-FLAG-mouse SNAP-25 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse SNAP-25 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15176).

Reference section:

Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15176_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF-1 alpha pro, FLAG, insert, hGCSF+p(A)
Sequence file: RDB15176_A7Fma.seq check
>D04709B2_1_A7Fm_EF1a-C_F_B03_06_ABI24.ab1
    1 TCAGCATGCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA
   61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG
  121 ATTCCACCAT GGACTACAAG GACGATGACG ACAAGGGATC CATGGCCGAG GACGCAGACA
  181 TGCGCAATGA GCTGGAGGAG ATGCAGAGGA GGGCTGACCA GCTGGCTGAT GAGTCCCTGG
  241 AAAGCACCCG TCGCATGCTG CAGCTGGTTG AAGAGAGTAA AGATGCTGGC ATCAGGACTT
  301 TGGTTATGTT GGATGAGCAA GGCGAACAAC TGGAACGCAT TGAGGAAGGG ATGGACCAAA
  361 TCAATAAGGA CATGAAAGAA GCAGAAAAGA ATTTGACGGA CCTAGGAAAA TTCTGCGGGC
  421 TTTGTGTGTG TCCCTGTAAC AAGCTTAAAT CCAGTGATGC TTACAAAAAA GCCTGGGGCA
  481 ATAATCAGGA TGGAGTAGTG GCCAGCCAGC CTGCCCGTGT GGTGGATGAA CGGGAGCAGA
  541 TGGCCATCAG TGGTGGCTTC ATCCGCAGGG TAACAAATGA TGCCCGGGAA AATGAGATGG
  601 ATGAGAACCT GGAGCAGGTG AGCGGCATCA TCGGAAACCT CCGCCATATG GCTCTAGACA
  661 TGGGCAATGA GATTGACACC CAGAATCGCC AGATCGACAG GATCATGGAG AAGGCTGATT
  721 CCAACAAAAC CAGAATTGAT GAAGCCAACC AACGTGCAAC AAAGATGCTG GGAAGTGGTT
  781 AATCACTAGT GAATTCGCGG CCGCTCTAGA GTGAGGGTCC CCACCTGGGA CCCTTGAGAG
  841 TATCAGTCTC CCACGTGGGA GACAAGAAAT CCCTGTTTAA TATTTAAACA GCAGTGTTCC
  901 CCATCTGGGT CCTTGCACCC CTCACTCTGG CCTCAGCCGA CTGCACAGCG GCCCCTGCAT
  961 CCCCTTGGCT GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT
 1021 GGGGTCCCAC GATTTGCTGG GGATCTCGTT TTTCTTCTAG ACTTTTGGGA CATGATTTGA
 1081 CTCCCGAACA TCAACGAACG TGTCTCTGCT TTTTCTGGAT GCTCGAACGC TGGTCTGGCC
1141 CCACGAGGTC AGACTGTGAC TCTTTTTTTA AGGGG
//
Primer: hGCSF-R (Pr0031)
Region: hGCSF+p(A), insert, FLAG, EF-1 alpha pro
Sequence file: RDB15176_A7Fmb.seq check
>D04709B2_1_A7Fm_hGCSF-R_C03_09_ABI24.ab1
    1 TGGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGAATT
   61 CACTAGTGAT TAACCACTTC CCAGCATCTT TGTTGCACGT TGGTTGGCTT CATCAATTCT
  121 GGTTTTGTTG GAATCAGCCT TCTCCATGAT CCTGTCGATC TGGCGATTCT GGGTGTCAAT
  181 CTCATTGCCC ATGTCTAGAG CCATATGGCG GAGGTTTCCG ATGATGCCGC TCACCTGCTC
  241 CAGGTTCTCA TCCATCTCAT TTTCCCGGGC ATCATTTGTT ACCCTGCGGA TGAAGCCACC
  301 ACTGATGGCC ATCTGCTCCC GTTCATCCAC CACACGGGCA GGCTGGCTGG CCACTACTCC
  361 ATCCTGATTA TTGCCCCAGG CTTTTTTGTA AGCATCACTG GATTTAAGCT TGTTACAGGG
  421 ACACACACAA AGCCCGCAGA ATTTTCCTAG GTCCGTCAAA TTCTTTTCTG CTTCTTTCAT
  481 GTCCTTATTG ATTTGGTCCA TCCCTTCCTC AATGCGTTCC AGTTGTTCGC CTTGCTCATC
  541 CAACATAACC AAAGTCCTGA TGCCAGCATC TTTACTCTCT TCAACCAGCT GCAGCATGCG
  601 ACGGGTGCTT TCCAGGGACT CATCAGCCAG CTGGTCAGCC CTCCTCTGCA TCTCCTCCAG
  661 CTCATTGCGC ATGTCTGCGT CCTCGGCCAT GGATCCCTTG TCGTCATCGT CCTTGTAGTC
  721 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA
  781 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA
  841 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC
  901 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA
  961 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA
 1021 CATGAAGCGA CGGCTGAGGA CGGGAAAGGC CCTTTTCCTT TTGTGTGGGT GACTCACCGG
 1081 CCCGCTCTCC CGAGCGCCGC GTCCTCCATT TTGAGCCTCC CTGCAGCCAG GGACGGAGCG
1141 GTCAATCTTT CCGCTCACGC AACTGGGTGG CCCGGACCCG GGGGCCAGAG CCTC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]