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pEF-FLAG-mouse VAMP-8

Expression vector of mouse vesicle-associated membrane protein 8 (VAMP-8).

Catalog number RDB15174
Resource name pEF-FLAG-mouse VAMP-8
Clone info. Expression vector of mouse vesicle-associated membrane protein 8 (VAMP-8), tagged with N-terminal FLAG, EF1 promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp8 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Vamp8 (NCBI Gene 22320) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 280 (47): 39175-39184, 2005)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15174 pEF-FLAG-mouse VAMP-8 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15174_A7Fmp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB15174_A7Fma.seq
>D04709A9_1_A7Fm_EF1a-C_F_G02_20_ABI24.ab1
    1 CCATTGGATG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGGAGG AGGCCAGTGG
  181 GAGTGCCGGA AATGACCGAG TTAGGAACCT GCAGAGTGAG GTGGAGGGAG TCAAGAATAT
  241 TATGACCCAG AATGTGGAGC GGATCTTGGC CAGAGGGGAG AACCTGGACC ACCTCCGAAA
  301 CAAGACAGAG GACTTGGAAG CCACGTCTGA ACACTTCAAG ACAACGTCCC AGAAGGTGGC
  361 CCGGAAGTTC TGGTGGAAGA ATGTGAAGAT GATTGTCATC ATCTGTGTGA TTGTCCTTAT
  421 CATCGTCATC CTCATTATAC TTTTTGCCAC TGGTACCATC CCCACTTAAA ATCACTAGTG
  481 AATTCGCGGC CGCTCTAGAG TGAGGGTCCC CACCTGGGAC CCTTGAGAGT ATCAGGTCTC
  541 CCACGTGGGA GACAAGAAAT CCCTGTTTAA TATTTAAACA GCAGTGTTCC CCATCTGGGT
  601 CCTTGCACCC CTCACTCTGG CCTCAGCCGA CTGCACAGCG GCCCCTGCAT CCCCTTGGCT
  661 GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT GGGGTCCCAC
  721 GAATTTGCTG GGGAATCTCG TTTTTCTTCT TAAGACTTTT GGGACATGGT TTGACTCCCG
  781 AACATCACCG ACGTGTCTCC TGTTTTTCTG GGTGGCCTCG GGACACCTGC CCTGCCCCCA
  841 CGAGGGTCAG GACTGTGACT CTTTTTAGGG CCAGGCAGGT GCCTGGACAT TTGCCTTGCT
  901 GGACGGGGAC TGGGGATGTG GGAGGGAGCA GACAGGAGGA ATCATGTCAG GCCTGTGTGT
  961 GAAAGGAAGC TCCACTGTCA CCCTCCACCT CTTCACCCCC CACTCACCAG TGTCCCCTCC
 1021 ACTGTCACAT TGTAACTGAA CTTCAGGATA TAAAGTGTTT GCCTCCTCAC AGAAATATAA
 1081 CGATAAAAAA ACAAAAGAAA AAGAAAAGAA AAAAAGAAAA ACACGCGGAA ATCTCTCTCT
1141 GTGGC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, M., Slp4-a/granuphilin-a interacts with syntaxin-2/3 in a Munc18-2-dependent manner. J. Biol. Chem. 280 (47): 39175-39184 (2005). PMID 16186111.

2019.03.15

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