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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse VAMP-2 (#RDB15171)

Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2).

Clone info. Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2), tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15171pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15171 pEF-FLAG-mouse VAMP-2 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse VAMP-2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15171).

Reference section:

Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15171_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1a pro, FLAG, insert, hGCSF+p(A)
Sequence file: RDB15171_A7Fma.seq check
>D04709A6_2_A7Fm_EF1a-C_F_C02_08_ABI24.ab1
    1 CCTGGGATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGTCGG CTACCGCTGC
  181 CACCGTCCCG CCTGCCGCCC CGGCCGGCGA GGGTGGCCCC CCTGCACCTC CTCCAAACCT
  241 TACTAGTAAC AGGAGACTGC AGCAGACCCA GGCCCAGGTG GATGAGGTGG TGGACATCAT
  301 GAGGGTGAAT GTGGACAAGG TCCTGGAGCG GGACCAGAAG TTGTCGGAGC TGGATGACCG
  361 TGCAGATGCC CTCCAGGCAG GGGCCTCCCA GTTTGAAACA AGTGCAGCCA AGCTCAAGCG
  421 CAAATACTGG TGGAAAAACC TCAAGATGAT GATCATCTTG GGAGTGATCT GCGCCATCAT
  481 CCTCATCATC ATCATCGTTT ACTTCAGCAC TTAAAATCAC TAGTGAATTC GCGGCCGCCT
  541 GCAGGTCGAC CATAGCGGCC GCTCTAGAGT GAGGGTCCCC ACCTGGGACC CTTGAGAGTA
  601 TCAGGTCTCC CACGTGGGAG ACAAGAAATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC
  661 CATCTGGGTC CTTGCACCCC TCACTCTGGC CTCAGCCGAC TGCACAGCGG CCCCTGCATC
  721 CCCTTGGCTG TGAGGCCCCT GGACAAGCAG AGGTGGCCAG AGCTGGGAGG CATGGCCCTG
  781 GGGTCCCACG AATTTGCTGG GGAATCTCGT TTTTCTTCTT AAGACTTTTG GGACATGGTT
  841 TGACTCCCGA ACATCACCGA CGTGTCTCCT GTTTTTCTGG GTGGCCTCGG GACACCTGCC
  901 CTGCCCCCAC GAGGGTCAGG ACTGTGACTC TTTTTAGGGC CAGGCAGGTG CCTGGACATT
  961 TGCCTTGCTG GACGGGGACT GGGGATGTGG GAGGGAGCAG ACAGGAGGAA TCATGTCAGG
 1021 CCTGTGTGTG AAAGGAAGCT CCACTGTCAC CCTCACTCTT CACCCCCCCA CCTCACCAGT
 1081 GTCCCCTCAC TGTCACATTG TAACTGAACG TCAGATTATT AAAGTGTTTG CCTCCGCAAA
1141 AGAAAAAAAC AGAACAGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]