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pEF-FLAG-mouse VAMP-2

Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2).

Catalog number RDB15171
Resource name pEF-FLAG-mouse VAMP-2
Clone info. Expression vector of mouse vesicle-associated membrane protein 2 (VAMP-2), tagged with N-terminal FLAG, EF1 promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Vamp2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Vamp2 (NCBI Gene 22318) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
Ordering Please visit Information of Request for Distribution.[link] 
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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15171 pEF-FLAG-mouse VAMP-2 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Nucleotide sequence (partial) provided by the depositor RDB15171pka01.seq

Original reference

original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15171_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F
Region: EF1a pro, FLAG, insert, hGCSF+p(A)
Sequence file: RDB15171_A7Fma.seq check
>D04709A6_2_A7Fm_EF1a-C_F_C02_08_ABI24.ab1
    1 CCTGGGATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCCATGTCGG CTACCGCTGC
  181 CACCGTCCCG CCTGCCGCCC CGGCCGGCGA GGGTGGCCCC CCTGCACCTC CTCCAAACCT
  241 TACTAGTAAC AGGAGACTGC AGCAGACCCA GGCCCAGGTG GATGAGGTGG TGGACATCAT
  301 GAGGGTGAAT GTGGACAAGG TCCTGGAGCG GGACCAGAAG TTGTCGGAGC TGGATGACCG
  361 TGCAGATGCC CTCCAGGCAG GGGCCTCCCA GTTTGAAACA AGTGCAGCCA AGCTCAAGCG
  421 CAAATACTGG TGGAAAAACC TCAAGATGAT GATCATCTTG GGAGTGATCT GCGCCATCAT
  481 CCTCATCATC ATCATCGTTT ACTTCAGCAC TTAAAATCAC TAGTGAATTC GCGGCCGCCT
  541 GCAGGTCGAC CATAGCGGCC GCTCTAGAGT GAGGGTCCCC ACCTGGGACC CTTGAGAGTA
  601 TCAGGTCTCC CACGTGGGAG ACAAGAAATC CCTGTTTAAT ATTTAAACAG CAGTGTTCCC
  661 CATCTGGGTC CTTGCACCCC TCACTCTGGC CTCAGCCGAC TGCACAGCGG CCCCTGCATC
  721 CCCTTGGCTG TGAGGCCCCT GGACAAGCAG AGGTGGCCAG AGCTGGGAGG CATGGCCCTG
  781 GGGTCCCACG AATTTGCTGG GGAATCTCGT TTTTCTTCTT AAGACTTTTG GGACATGGTT
  841 TGACTCCCGA ACATCACCGA CGTGTCTCCT GTTTTTCTGG GTGGCCTCGG GACACCTGCC
  901 CTGCCCCCAC GAGGGTCAGG ACTGTGACTC TTTTTAGGGC CAGGCAGGTG CCTGGACATT
  961 TGCCTTGCTG GACGGGGACT GGGGATGTGG GAGGGAGCAG ACAGGAGGAA TCATGTCAGG
 1021 CCTGTGTGTG AAAGGAAGCT CCACTGTCAC CCTCACTCTT CACCCCCCCA CCTCACCAGT
 1081 GTCCCCTCAC TGTCACATTG TAACTGAACG TCAGATTATT AAAGTGTTTG CCTCCGCAAA
1141 AGAAAAAAAC AGAACAGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209.

2021.05.03

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