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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse syntaxin-1a (#RDB15166)

Expression vector of mouse syntaxin-1A [Stx1a in NCBI].

Clone info. Expression vector of mouse syntaxin-1A [Stx1a in NCBI], tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pEF-BOS FLAG (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Stx1a cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15166pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15166 pEF-FLAG-mouse syntaxin-1a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-FLAG-mouse syntaxin-1a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15166).

Reference section:

Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15166_A7Fmp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1a pro, FLAG tag, insert 5'
Sequence file: RDB15166_A7Fma.seq check
>D04709A1_1_A7Fm_EF1a-C_F_A01_01_ABI24.ab1
    1 TAATGGATTG CCTTTTTGAG TTTGGATCTT GGTTCATTCT CAAGCCTCAG ACAGTGGTTC
   61 AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGAGCGGC CGCGGGAATT
  121 CGATTCCACC ATGGACTACA AGGACGATGA CGACAAGGGA TCTATGAAGG ACCGAACCCA
  181 GGAGCTCCGC ACGGCCAAGG ACAGCGATGA CGACGACGAT GTCACTGTCA CTGTGGACCG
  241 AGACCGCTTC ATGGATGAAT TCTTTGAACA GGTGGAAGAG ATCCGGGGCT TTATTGACAA
  301 GATTGCCGAA AACGTGGAGG AGGTGAAGCG GAAACACAGC GCCATCCTGG CCTCCCCGAA
  361 CCCCGATGAG AAGACAAAGG AGGAACTGGA GGAGCTCATG TCGGACATTA AGAAGACAGC
  421 GAACAAAGTT CGCTCCAAGC TAAAGAGCAT TGAGCAGAGC ATCGAGCAGG AGGAAGGTCT
  481 GAACCGCTCA TCAGCCGACC TGAGGATCCG GAAGACGCAG CACTCCACGC TGTCCCGAAA
  541 GTTTGTGGAG GTCATGTCCG AGTACAACGC CACTCAGTCA GACTACCGAG AACGCTGCAA
  601 AGGGCGCATC CAGAGGCAGC TGGAGATCAC CGGCCGGACC ACGACCAGTG AGGAATTGGA
  661 AGACATGCTG GAGAGTGGGA ACCCTGCCAT CTTTGCCTCT GGGATCATCA TGGACTCCAG
  721 CATCTCGAAG CAGGCCCTCA GTGAGATCGA GACCAGACAC AGTGAGATCA TCAAGCTGGA
  781 GACCAGCATC CGGGAGCTGC ACGACATGTT CATGGACATG GCCATGCTGG TGGAGAGCCA
  841 GGGGGAGATG ATTGACAGGA TCGAGTACAA TGTGGAGCAC GCCGTGGACT ACGTGGAGAG
  901 GGCCGTGTCA GACACCAAGA AAGGCCGTCA AGTACCAGAG CAAGGCGCGC AGGAAGAAGA
  961 TCATGATCAT CATTTGCTGT GTGATTCTGG GCATCATCAT CGCCTCCACC ATCGGGGGCA
 1021 TCTTTGGATA GAATCACTAG TGAAATTCGC GGGCCGCCTG CAGGTCGACC ATAGCGGCCG
 1081 CCTCTAGAGT GAGGGTCCCC CACCTGGGAC CATGGAGAGT ATCAAGTCTC CCAACGTGGG
1141 AGACAAAGAA ATCCCCTTGG ATTTTAAATA GT
//
Primer: hGCSF-R (Pr0031)
Region: hGCSF+p(A), insert, FLAG tag
Sequence file: RDB15166_A7Fmb.seq check
>D04709A1_1_A7Fm_hGCSF-R_B01_04_ABI24.ab1
    1 GGGGGGAAAT GAATCTCTCA GGGTCCCAGG TGGGGACCCT CACTCTAGAG CGGCCGCTAT
   61 GGTCGACCTG CAGGCGGCCG CGAATTCACT AGTGATTCTA TCCAAAGATG CCCCCGATGG
  121 TGGAGGCGAT GATGATGCCC AGAATCACAC AGCAAATGAT GATCATGATC TTCTTCCTGC
  181 GCGCCTTGCT CTGGTACTTG ACGGCCTTCT TGGTGTCTGA CACGGCCCTC TCCACGTAGT
  241 CCACGGCGTG CTCCACATTG TACTCGATCC TGTCAATCAT CTCCCCCTGG CTCTCCACCA
  301 GCATGGCCAT GTCCATGAAC ATGTCGTGCA GCTCCCGGAT GCTGGTCTCC AGCTTGATGA
  361 TCTCACTGTG TCTGGTCTCG ATCTCACTGA GGGCCTGCTT CGAGATGCTG GAGTCCATGA
  421 TGATCCCAGA GGCAAAGATG GCAGGGTTCC CACTCTCCAG CATGTCTTCC AATTCCTCAC
  481 TGGTCGTGGT CCGGCCGGTG ATCTCCAGCT GCCTCTGGAT GCGCCCTTTG CAGCGTTCTC
  541 GGTAGTCTGA CTGAGTGGCG TTGTACTCGG ACATGACCTC CACAAACTTT CGGGACAGCG
  601 TGGAGTGCTG CGTCTTCCGG ATCCTCAGGT CGGCTGATGA GCGGTTCAGA CCTTCCTCCT
  661 GCTCGATGCT CTGCTCAATG CTCTTTAGCT TGGAGCGAAC TTTGTTCGCT GTCTTCTTAA
  721 TGTCCGACAT GAGCTCCTCC AGTTCCTCCT TTGTCTTCTC ATCGGGGTTC GGGGAGGCCA
  781 GGATGGCGCT GTGTTTCCGC TTCACCTCCT CCACGTTTTC GGCAATCTTG TCAATAAAGC
  841 CCCGGATCTC TTCCACCTGT TCAAAGAATT CATCCATGAA GCGGTCTCGG TCCACAGTGA
  901 CAGTGACATC GTCGTCGTCA TCGCTGTCCT TGGCCGTGCG GAGCTCCTGG GTTCGGTCCT
  961 TCATAGATCC CTTGTCGTCA TCGTCCTTGT AGTCCATGGT GGAATCGAAT TCCCGCGGGC
 1021 CGCTCTAGAG AATTCCTCAC GACACCTGAA ATGGGAAGAA AAAAAACTTT GACCACTGTC
 1081 TGAGGCTTGA GAATGACCAG ATCCAACTCA AAAAGGCATT CAGGAGATAC ATCAGGTGCC
1141 AAGCTGGCCT AACTTCAGTC CTCAACCCCA ACTTCAAGA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Fukuda, M., Vesicle-associated membrane protein-2/synaptobrevin binding to synaptotagmin I promotes O-glycosylation of synaptotagmin I. J. Biol. Chem. 277 (33): 30351-30358 (2002). PMID 12048209. [link to RRC of NBRP]