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pcDNA3-hNEK7 (#RDB15165)

Expression vector of human NIMA related kinase 7 (NEK7), HA-tag.

Clone info. Expression vector of human NIMA related kinase 7 (NEK7), tagged with N-terminal HA.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Amp, 37oC
Gene/insert name human NEK7 cDNA
Depositor|Developer Ishikawa, Fuyuki |
 
Sequence (full) RDB15165hts01.seq checkAssembled from experimentally sequenced data.
Publication Hirai, Y., Genes Cells 21 (8): 874-889 (2016). PMID 27396482. [link to RRC of NBRP]
Test sheet RDB15165C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Genes Cells 21 (8), 874-889, 2016)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Genes Cells 21 (8), 874-889, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15165 pcDNA3-hNEK7 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pcDNA3-hNEK7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15165).

Reference section:

Hirai, Y., Tamura, M., Otani, J., Ishikawa, F., NEK6-mediated phosphorylation of human TPP1 regulates telomere length through telomerase recruitment. Genes Cells 21 (8): 874-889 (2016). PMID 27396482. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Hirai, Y., NEK6-mediated phosphorylation of human TPP1 regulates telomere length through telomerase recruitment. Genes Cells 21 (8): 874-889 (2016). PMID 27396482. [link to RRC of NBRP]