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mCherry-EF1A1

Expression vector of rat Elongation factor 1-alpha 1 (EF1A1), mCherry tag.

Catalog number RDB15157
Resource name mCherry-EF1A1
Clone info. Expression vector of rat Elongation factor 1-alpha 1 (EF1A1) with N-terminally tagged mCherry.
Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Eef1a1 cDNA
Depositor Hamachi, Itaru |
 
Other clones in our bank rat Eef1a1 (KEGG orthology K03231) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15157 mCherry-EF1A1 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15157_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F RDB15157_A7Fla.seq
>D04425A6_A7Fl_2_mCherry-C_F_D09_12_ABI24.ab1
    1 TGACAGGTAG AGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT
   61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA
  121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TCGAGCTATG GGAAAGGAAA
  181 AGACTCACAT CAACATCGTC GTAATTGGAC ACGTAGATTC CGGCAAGTCC ACCACAACCG
  241 GCCACCTGAT CTACAAATGT GGTGGAATCG ACAAAAGAAC CATTGAAAAG TTTGAGAAGG
  301 AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG GGTCCTGGAC AAACTGAAAG
  361 CTGAGCGTGA GCGTGGTATC ACTATTGACA TCTCCCTGTG GAAGTTTGAG ACCAGCAAGT
  421 ACTATGTGAC TATCATTGAT GCCCCAGGAC ACAGAGACTT CATCAAGAAC ATGATTACGG
  481 GCACATCTCA GGCTGACTGC GCTGTCCTGA TTGTTGCTGC TGGTGTTGGG GAATTTGAAG
  541 CTGGTATCTC CAAGAACGGG CAGACCCGTG AGCATGCTCT TCTGGCTTAC ACTTTGGGTG
  601 TGAAACAGCT AATTGTTGGT GTCAACAAAA TGGATTCCAC CGAGCCACCA TACAGTCAGA
  661 AGAGATACGA GGAAATCGTT AAGGAAGTCA GCACCTACAT TAAGAAAATT GGCTACAACC
  721 CTGACACAGT AGCATTTGTG CCAATTTCTG GTTGGAATGG TGACAACATG CTGGAGCCAA
  781 GTGCTAATAT GCCGTGGTTC AAGGGATGGA AAGTCACCCG CAAAGATGGC AGTGCCAGTG
  841 GCACCACGCT GCTGGAAGCT TTGGACTGCA TTCTGCCGCC AACTCGTCCA ACTGACAAGC
  901 CTCTGCGACT GCCCCTCCAG GATGTCTATA AAATTGGCGG CATTGGCACT GTCCCTGTGG
  961 GCCGAGTGGA AACTGGTGTT TCTCAAACCT GGTATGGGTG GTTACCCTTT GCTCCAGTCA
 1021 ATGTTAACAC TGAAGTCAAG TCTGTGGAAA TTGCACATGC AGGCTTTGAG TGAAGCTCTG
1081 CCTGGGCACG ATTGTAAGCC TTCCAACGGT AAAAGAAACC GGGTGGT
//
Primer: SV40polyA-R RDB15157_A7Flb.seq
>D04425A6_A7Fl_2_SV40polyA-R_E09_15_ABI24.ab1
    1 AAAAGTTGGG AAGTTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
   61 TCAGTTATCT AGATCCGGTG GATCCTCATT TAGCCTTCTG AGCTTTCTGG GCAGACTTGG
  121 TGACTTTGCC AGCTCCTGCA GCCTTCTTGT CCACGGCTTT GATGACACCC ACAGCAACTG
  181 TCTGCCTCAT GTCACGAACA GCAAAACGAC CAAGTGGAGG GTAGTCAGAG AAGCTTTCAA
  241 CACACATGGG CTTGCCAGGG ACCATGTCAA CAATGGCAGC ATCACCAGAC TTCAAGAATT
  301 TGGGGCCATC TTCCAGCTTC TTACCAGAAC GACGATCGAT CTTCTCTTTA AGCTCGGCAA
  361 ACTTGCATGC TATGTGGGCC GTGTGGCAGT CCAGAACAGG GGCATAGCCA GCACTGATCT
  421 GGCCTGGATG GTTCAGGATA ATCACCTGAG CAGTGAAGCC AGCTGCTTCC ATTGGTGGGT
  481 CATTTTTGCT GTCCCCAGCA ACATTGCCAC GTCTAACGTC TTTGACAGAC ACGTTCTTTA
  541 CGTTGAAGCC TACATTGTCC CCAGGCAGAG CTTCACTCAA AGCTTCATGG TGCATTTCCA
  601 CAGACTTGAC TTCAGTTGTT ACATTGACTG GAGCAAAGGT AACCACCATA CCAGGTTTGA
  661 GAACACCAGT TTCCACTCGG CCCACAGGGA CAGTGCCAAT GCCGCCAATT TTATAGACAT
  721 CCTGGAGGGG CAGTCGCAGA GGCTTGTCAG TTGGACGAGT TGGCGGCAGA ATGCAGTCCA
  781 AAGCTTCCAG CAGCGTGGTG CCACTGGCAC TGCCATCTTT GCGGGTGACT TTCCATCCCT
  841 TGAACCACGG CATATTAGCA CTTGGCTCCA GCATGTTGTC ACCATTCCAA CCAGAAAATT
  901 GGCACAAATG CTACTGTGTC AAGGGTTGTA GCCAATTTTC TTAATGTAGG TGCTGACTTC
  961 CTTAACGAAT TTTCCCTCGT AATCTCTTCT GACTGTATGG GTGGGCTCGG TGGAAATCCA
 1021 ATTTTTTGGT TGACAACCAA CAAATTTAAG CTTGATTCAC AACCCAAAAG TGTAAGTCAG
1081 AGAGCCATGC TTCAACGGTC TGGCCCCGGA TTTCCTTTGG GAG
//
Primer: CMV-Forward RDB15157_A7Flc.seq
>D04425A6_A7Fl_2_CMV-Forward_C09_09_ABI24.ab1
    1 CTGATCCCTT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG
  121 GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG
  181 CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC
  241 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC
  301 GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC
  361 GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG
  421 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC
  481 GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG
  541 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC
  601 CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG
  661 GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC
  721 ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC
  781 GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT
  841 CGAGCTATGG GAAAGGAAAA GACTCACATC AACATCGTCG TAATTGGACA CGTAGATTCC
  901 GGCAAGTCCA CCACAACCGG CCACCTGATC TACAAATGTG GTGGAATCGA CAAAAGAACC
  961 ATTGAAAAGT TTGAGAAAGG AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG
 1021 GTCCTGCACA ACTGAAAGCT GAACGTGAGC GTGGTATCAC TATTGACATC TCCCTGTGGA
1081 GTTTGAGACC AGCCAAGGCT ACCTAAG
//
Primer: SV40pro_F_V2 RDB15157_A7Fld.seq
>D04425A6_A7Fl_2_SV40pro_F_V2_F09_18_ABI24.ab1
    1 TCCCAACTTT TTTTGGGAGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT
   61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA
  601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCCAACC TGCCATCACG AGATTTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
  961 GCTTCGGAAT CGTTTTCCGG GGACGCCGGC TGGATGATCC TTCCAGCGCG GGGAATCTCA
 1021 TGCTGAGGTT CTTTCGCCCC ACCCTAAGGG GAGGCTAACT GAAACAACGG AAGGAGACCA
1081 ATACCGGAAG GATCCCGGCC GCTTATTGAA CC
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391.

2018.11.21

GNP_filter3_RDBDEP_html_181012.pl