Please review the QC test results indicated by icon below as well as clone information before placing your order.

mCherry-EF1A1

Expression vector of rat Elongation factor 1-alpha 1 (EF1A1), mCherry tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15157_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mCherry-C_F Sequence file: RDB15157_A7Fla.seq
>D04425A6_A7Fl_2_mCherry-C_F_D09_12_ABI24.ab1     1 TGACAGGTAG AGTGCAGCTG CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT    61 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA   121 CCGGCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TCGAGCTATG GGAAAGGAAA   181 AGACTCACAT CAACATCGTC GTAATTGGAC ACGTAGATTC CGGCAAGTCC ACCACAACCG   241 GCCACCTGAT CTACAAATGT GGTGGAATCG ACAAAAGAAC CATTGAAAAG TTTGAGAAGG   301 AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG GGTCCTGGAC AAACTGAAAG   361 CTGAGCGTGA GCGTGGTATC ACTATTGACA TCTCCCTGTG GAAGTTTGAG ACCAGCAAGT   421 ACTATGTGAC TATCATTGAT GCCCCAGGAC ACAGAGACTT CATCAAGAAC ATGATTACGG   481 GCACATCTCA GGCTGACTGC GCTGTCCTGA TTGTTGCTGC TGGTGTTGGG GAATTTGAAG   541 CTGGTATCTC CAAGAACGGG CAGACCCGTG AGCATGCTCT TCTGGCTTAC ACTTTGGGTG   601 TGAAACAGCT AATTGTTGGT GTCAACAAAA TGGATTCCAC CGAGCCACCA TACAGTCAGA   661 AGAGATACGA GGAAATCGTT AAGGAAGTCA GCACCTACAT TAAGAAAATT GGCTACAACC   721 CTGACACAGT AGCATTTGTG CCAATTTCTG GTTGGAATGG TGACAACATG CTGGAGCCAA   781 GTGCTAATAT GCCGTGGTTC AAGGGATGGA AAGTCACCCG CAAAGATGGC AGTGCCAGTG   841 GCACCACGCT GCTGGAAGCT TTGGACTGCA TTCTGCCGCC AACTCGTCCA ACTGACAAGC   901 CTCTGCGACT GCCCCTCCAG GATGTCTATA AAATTGGCGG CATTGGCACT GTCCCTGTGG   961 GCCGAGTGGA AACTGGTGTT TCTCAAACCT GGTATGGGTG GTTACCCTTT GCTCCAGTCA  1021 ATGTTAACAC TGAAGTCAAG TCTGTGGAAA TTGCACATGC AGGCTTTGAG TGAAGCTCTG 1081 CCTGGGCACG ATTGTAAGCC TTCCAACGGT AAAAGAAACC GGGTGGT //
Primer: SV40polyA-R Sequence file: RDB15157_A7Flb.seq
>D04425A6_A7Fl_2_SV40polyA-R_E09_15_ABI24.ab1     1 AAAAGTTGGG AAGTTTTTTA AGCAAGTAAA CCTCTACAAA TGTGGTATGG CTGATTATGA    61 TCAGTTATCT AGATCCGGTG GATCCTCATT TAGCCTTCTG AGCTTTCTGG GCAGACTTGG   121 TGACTTTGCC AGCTCCTGCA GCCTTCTTGT CCACGGCTTT GATGACACCC ACAGCAACTG   181 TCTGCCTCAT GTCACGAACA GCAAAACGAC CAAGTGGAGG GTAGTCAGAG AAGCTTTCAA   241 CACACATGGG CTTGCCAGGG ACCATGTCAA CAATGGCAGC ATCACCAGAC TTCAAGAATT   301 TGGGGCCATC TTCCAGCTTC TTACCAGAAC GACGATCGAT CTTCTCTTTA AGCTCGGCAA   361 ACTTGCATGC TATGTGGGCC GTGTGGCAGT CCAGAACAGG GGCATAGCCA GCACTGATCT   421 GGCCTGGATG GTTCAGGATA ATCACCTGAG CAGTGAAGCC AGCTGCTTCC ATTGGTGGGT   481 CATTTTTGCT GTCCCCAGCA ACATTGCCAC GTCTAACGTC TTTGACAGAC ACGTTCTTTA   541 CGTTGAAGCC TACATTGTCC CCAGGCAGAG CTTCACTCAA AGCTTCATGG TGCATTTCCA   601 CAGACTTGAC TTCAGTTGTT ACATTGACTG GAGCAAAGGT AACCACCATA CCAGGTTTGA   661 GAACACCAGT TTCCACTCGG CCCACAGGGA CAGTGCCAAT GCCGCCAATT TTATAGACAT   721 CCTGGAGGGG CAGTCGCAGA GGCTTGTCAG TTGGACGAGT TGGCGGCAGA ATGCAGTCCA   781 AAGCTTCCAG CAGCGTGGTG CCACTGGCAC TGCCATCTTT GCGGGTGACT TTCCATCCCT   841 TGAACCACGG CATATTAGCA CTTGGCTCCA GCATGTTGTC ACCATTCCAA CCAGAAAATT   901 GGCACAAATG CTACTGTGTC AAGGGTTGTA GCCAATTTTC TTAATGTAGG TGCTGACTTC   961 CTTAACGAAT TTTCCCTCGT AATCTCTTCT GACTGTATGG GTGGGCTCGG TGGAAATCCA  1021 ATTTTTTGGT TGACAACCAA CAAATTTAAG CTTGATTCAC AACCCAAAAG TGTAAGTCAG 1081 AGAGCCATGC TTCAACGGTC TGGCCCCGGA TTTCCTTTGG GAG //
Primer: CMV-Forward Sequence file: RDB15157_A7Flc.seq
>D04425A6_A7Fl_2_CMV-Forward_C09_09_ABI24.ab1     1 CTGATCCCTT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTGGTTTAG TGAACCGTCA GATCCGCTAG CGCTACCGGT CGCCACCATG   121 GTGAGCAAGG GCGAGGAGGA TAACATGGCC ATCATCAAGG AGTTCATGCG CTTCAAGGTG   181 CACATGGAGG GCTCCGTGAA CGGCCACGAG TTCGAGATCG AGGGCGAGGG CGAGGGCCGC   241 CCCTACGAGG GCACCCAGAC CGCCAAGCTG AAGGTGACCA AGGGTGGCCC CCTGCCCTTC   301 GCCTGGGACA TCCTGTCCCC TCAGTTCATG TACGGCTCCA AGGCCTACGT GAAGCACCCC   361 GCCGACATCC CCGACTACTT GAAGCTGTCC TTCCCCGAGG GCTTCAAGTG GGAGCGCGTG   421 ATGAACTTCG AGGACGGCGG CGTGGTGACC GTGACCCAGG ACTCCTCCCT GCAGGACGGC   481 GAGTTCATCT ACAAGGTGAA GCTGCGCGGC ACCAACTTCC CCTCCGACGG CCCCGTAATG   541 CAGAAGAAGA CCATGGGCTG GGAGGCCTCC TCCGAGCGGA TGTACCCCGA GGACGGCGCC   601 CTGAAGGGCG AGATCAAGCA GAGGCTGAAG CTGAAGGACG GCGGCCACTA CGACGCTGAG   661 GTCAAGACCA CCTACAAGGC CAAGAAGCCC GTGCAGCTGC CCGGCGCCTA CAACGTCAAC   721 ATCAAGTTGG ACATCACCTC CCACAACGAG GACTACACCA TCGTGGAACA GTACGAACGC   781 GCCGAGGGCC GCCACTCCAC CGGCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCT   841 CGAGCTATGG GAAAGGAAAA GACTCACATC AACATCGTCG TAATTGGACA CGTAGATTCC   901 GGCAAGTCCA CCACAACCGG CCACCTGATC TACAAATGTG GTGGAATCGA CAAAAGAACC   961 ATTGAAAAGT TTGAGAAAGG AGGCTGCGGA GATGGGAAAA GGCTCCTTCA AGTATGCCTG  1021 GTCCTGCACA ACTGAAAGCT GAACGTGAGC GTGGTATCAC TATTGACATC TCCCTGTGGA 1081 GTTTGAGACC AGCCAAGGCT ACCTAAG //
Primer: SV40pro_F_V2 Sequence file: RDB15157_A7Fld.seq
>D04425A6_A7Fl_2_SV40pro_F_V2_F09_18_ABI24.ab1     1 TCCCAACTTT TTTTGGGAGC CTAGGCTTTT GCAAGATCGA TCAAGAGACA GGATGAGGAT    61 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   121 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   181 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   241 ATGAACTGCA AGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   301 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   361 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   421 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   481 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   541 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGAGCA   601 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   661 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   721 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   781 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   841 GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC TCTGGGGGTT CGAAATGACC GACCAAGCGA   901 CGCCCCAACC TGCCATCACG AGATTTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG   961 GCTTCGGAAT CGTTTTCCGG GGACGCCGGC TGGATGATCC TTCCAGCGCG GGGAATCTCA  1021 TGCTGAGGTT CTTTCGCCCC ACCCTAAGGG GAGGCTAACT GAAACAACGG AAGGAGACCA 1081 ATACCGGAAG GATCCCGGCC GCTTATTGAA CC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl