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PPIA-mCherry (#RDB15155)

Expression vector of rat Peptidyl-prolyl cis-trans isomerase A (PPIA), mCherry tag.

Clone info. Expression vector of rat Peptidyl-prolyl cis-trans isomerase A (PPIA) with C-terminally tagged mCherry.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Ppia cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15155 PPIA-mCherry DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The PPIA-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15155).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15155_A7Flp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15155_A7Fla.seq check
>D04425A4_A7Fl_1_CMV-Forward_E08_14_ABI24.ab1
    1 CCTAACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGGCC
  121 ACCATGGTCA ACCCCACCGT GTTCTTCGAC ATCACGGCTG ATGGCGAGCC CTTGGGTCGC
  181 GTCTGCTTCG AGCTGTTTGC AGACAAAGTT CCAAAGACAG CAGAAAACTT TCGTGCTCTG
  241 AGCACTGGGG AGAAAGGATT TGGCTATAAG GGTTCCTCCT TTCACAGAAT TATTCCAGGA
  301 TTCATGTGCC AGGGTGGTGA CTTCACACGC CATAATGGCA CTGGTGGCAA GTCCATCTAC
  361 GGAGAGAAAT TTGAGGATGA GAACTTCATC CTGAAGCATA CAGGTCCTGG CATCTTGTCC
  421 ATGGCAAATG CTGGACCAAA CACAAATGGT TCCCAGTTTT TTATCTGCAC TGCCAAGACT
  481 GAGTGGCTGG ATGGCAAGCA TGTGGTCTTT GGGAAGGTGA AAGAAGGCAT GAGCATTGTG
  541 GAAGCCATGG AGCGTTTTGG GTCCAGGAAT GGCAAGACCA GCAAGAAGAT CACCATCTCC
  601 GACTGTGGAC AACTCGTGGA TCCACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT
  661 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC
  721 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
  781 GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT
  841 CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG
  901 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGGCGG
  961 CGTGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CCAGGTGAGC
 1021 CTGCCGCGGG CACCAACGTC TCCATCGGAC CGACCCCGTA ATGCAGAGAG ATCATGAGCC
1081 TGGGAGTCGT CATTCCGTAG CCGGGAT
//
Primer: SV40polyA-R
Sequence file: RDB15155_A7Flb.seq check
>D04425A4_A7Fl_1_SV40polyA-R_F08_17_ABI24.ab1
    1 AAAAATTGGA GTTTTTAAGC AGTAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA
   61 GAGTCGCGGC CGCTACTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC
  121 GCGTTCGTAC TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT
  181 GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC
  241 CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG
  301 GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG
  361 CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC
  421 GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT
  481 CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC AAGTAGTCGG GGATGTCGGC
  541 GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGC
  601 GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG
  661 GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CCTCCATGTG
  721 CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA TCCTCCTCGC CCTTGCTCAC
  781 CATGGTGGCG ACCGGTGGAT CCACGAGTTG TCCACAGTCG GAGATGGTGA TCTTCTTGCT
  841 GGTCTTGCCA TTCCTGGACC CAAAACGCTC CATGGCTTCC ACAATGCTCA TGCCTTCTTT
  901 CACCTTCCCA AAGACCACAT GCTTGCCATC CAGCCACTCA GTCTTGGCAG TGCAGATAAA
  961 AAAACTGGGA AACCATTTTG TGTTTGGTCC AGCATTTTGC CATGGACAAG ATGCCAGGAC
 1021 CCTGTATGCT TCAGATGAAA GTTCTCATCC TCAAATTTTC TCCTCCGTAG ATTGACTGGC
1081 CACCAGGTTG GTCATTAATG GGCGGTGGTG GAAAAGTC
//
Primer: SV40pro_F_V2
Sequence file: RDB15155_A7Flc.seq check
>D04425A4_A7Fl_1_SV40pro_F_V2_G08_20_ABI24.ab1
    1 GTCAACTTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCCTTC TATGAAAGGT TGGGCTTCGG
  961 AATCGTTTTT CCGGGACGCC CGGCTGGGAT GATCCTCCAG CGCGGGGGAT CTCATGCTGG
 1021 AGTTTCTTTC GCCCACCCCT TAGGGGGAAG GCTACTGGAA CACGAAGAGA CGATACGGGA
1081 AGAACCCGCG CGCCTATTGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]