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PPIA-mCherry

Expression vector of rat Peptidyl-prolyl cis-trans isomerase A (PPIA), mCherry tag.

Catalog number RDB15155
Resource name PPIA-mCherry
Clone info. Expression vector of rat Peptidyl-prolyl cis-trans isomerase A (PPIA) with C-terminally tagged mCherry.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy.
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Ppia cDNA
Depositor|Developer Hamachi, Itaru |
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External Database
rat Ppia

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
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          RDB15155 PPIA-mCherry DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The PPIA-mCherry was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15155).

          Reference section:

          Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15155_A7Flp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB15155_A7Fla.seq check
          >D04425A4_A7Fl_1_CMV-Forward_E08_14_ABI24.ab1
              1 CCTAACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGGCC
            121 ACCATGGTCA ACCCCACCGT GTTCTTCGAC ATCACGGCTG ATGGCGAGCC CTTGGGTCGC
            181 GTCTGCTTCG AGCTGTTTGC AGACAAAGTT CCAAAGACAG CAGAAAACTT TCGTGCTCTG
            241 AGCACTGGGG AGAAAGGATT TGGCTATAAG GGTTCCTCCT TTCACAGAAT TATTCCAGGA
            301 TTCATGTGCC AGGGTGGTGA CTTCACACGC CATAATGGCA CTGGTGGCAA GTCCATCTAC
            361 GGAGAGAAAT TTGAGGATGA GAACTTCATC CTGAAGCATA CAGGTCCTGG CATCTTGTCC
            421 ATGGCAAATG CTGGACCAAA CACAAATGGT TCCCAGTTTT TTATCTGCAC TGCCAAGACT
            481 GAGTGGCTGG ATGGCAAGCA TGTGGTCTTT GGGAAGGTGA AAGAAGGCAT GAGCATTGTG
            541 GAAGCCATGG AGCGTTTTGG GTCCAGGAAT GGCAAGACCA GCAAGAAGAT CACCATCTCC
            601 GACTGTGGAC AACTCGTGGA TCCACCGGTC GCCACCATGG TGAGCAAGGG CGAGGAGGAT
            661 AACATGGCCA TCATCAAGGA GTTCATGCGC TTCAAGGTGC ACATGGAGGG CTCCGTGAAC
            721 GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG CACCCAGACC
            781 GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCG CCTGGGACAT CCTGTCCCCT
            841 CAGTTCATGT ACGGCTCCAA GGCCTACGTG AAGCACCCCG CCGACATCCC CGACTACTTG
            901 AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA GGACGGGCGG
            961 CGTGTGACCG TGACCCAGGA CTCCTCCCTG CAGGACGGCG AGTTCATCTA CCAGGTGAGC
           1021 CTGCCGCGGG CACCAACGTC TCCATCGGAC CGACCCCGTA ATGCAGAGAG ATCATGAGCC
          1081 TGGGAGTCGT CATTCCGTAG CCGGGAT
          //
          Primer: SV40polyA-R
          Sequence file: RDB15155_A7Flb.seq check
          >D04425A4_A7Fl_1_SV40polyA-R_F08_17_ABI24.ab1
              1 AAAAATTGGA GTTTTTAAGC AGTAAACCTC TACAAATGTG GTATGGCTGA TTATGATCTA
             61 GAGTCGCGGC CGCTACTTGT ACAGCTCGTC CATGCCGCCG GTGGAGTGGC GGCCCTCGGC
            121 GCGTTCGTAC TGTTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAACTTGAT
            181 GTTGACGTTG TAGGCGCCGG GCAGCTGCAC GGGCTTCTTG GCCTTGTAGG TGGTCTTGAC
            241 CTCAGCGTCG TAGTGGCCGC CGTCCTTCAG CTTCAGCCTC TGCTTGATCT CGCCCTTCAG
            301 GGCGCCGTCC TCGGGGTACA TCCGCTCGGA GGAGGCCTCC CAGCCCATGG TCTTCTTCTG
            361 CATTACGGGG CCGTCGGAGG GGAAGTTGGT GCCGCGCAGC TTCACCTTGT AGATGAACTC
            421 GCCGTCCTGC AGGGAGGAGT CCTGGGTCAC GGTCACCACG CCGCCGTCCT CGAAGTTCAT
            481 CACGCGCTCC CACTTGAAGC CCTCGGGGAA GGACAGCTTC AAGTAGTCGG GGATGTCGGC
            541 GGGGTGCTTC ACGTAGGCCT TGGAGCCGTA CATGAACTGA GGGGACAGGA TGTCCCAGGC
            601 GAAGGGCAGG GGGCCACCCT TGGTCACCTT CAGCTTGGCG GTCTGGGTGC CCTCGTAGGG
            661 GCGGCCCTCG CCCTCGCCCT CGATCTCGAA CTCGTGGCCG TTCACGGAGC CCTCCATGTG
            721 CACCTTGAAG CGCATGAACT CCTTGATGAT GGCCATGTTA TCCTCCTCGC CCTTGCTCAC
            781 CATGGTGGCG ACCGGTGGAT CCACGAGTTG TCCACAGTCG GAGATGGTGA TCTTCTTGCT
            841 GGTCTTGCCA TTCCTGGACC CAAAACGCTC CATGGCTTCC ACAATGCTCA TGCCTTCTTT
            901 CACCTTCCCA AAGACCACAT GCTTGCCATC CAGCCACTCA GTCTTGGCAG TGCAGATAAA
            961 AAAACTGGGA AACCATTTTG TGTTTGGTCC AGCATTTTGC CATGGACAAG ATGCCAGGAC
           1021 CCTGTATGCT TCAGATGAAA GTTCTCATCC TCAAATTTTC TCCTCCGTAG ATTGACTGGC
          1081 CACCAGGTTG GTCATTAATG GGCGGTGGTG GAAAAGTC
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB15155_A7Flc.seq check
          >D04425A4_A7Fl_1_SV40pro_F_V2_G08_20_ABI24.ab1
              1 GTCAACTTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
             61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
            601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
            901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCCTTC TATGAAAGGT TGGGCTTCGG
            961 AATCGTTTTT CCGGGACGCC CGGCTGGGAT GATCCTCCAG CGCGGGGGAT CTCATGCTGG
           1021 AGTTTCTTTC GCCCACCCCT TAGGGGGAAG GCTACTGGAA CACGAAGAGA CGATACGGGA
          1081 AGAACCCGCG CGCCTATTGG A
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl