Please review the QC test results indicated by icon below as well as clone information before placing your order.

ENPL-mCherry-KDEL

Expression vector of rat Endoplasmin (ENPL), mCherry-ER retention signal (KDEL) tag.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
Remarks	This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Methods 13 (11): 931-937, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
備考	Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15154_A7Flp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB15154_A7Fla.seq
>D04425A3_A7Fl_2_CMV-Forward_A08_02_ABI24.ab1     1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGGCC   121 ACCATGAGGG TCCTGTGGGT GCTGGGCCTC TGCTGCGTCC TGCTGACCTT CGGGTTTGTG   181 AGAGCTGACG ATGAAGTCGA TGTGGATGGC ACGGTAGAAG AGGACCTGGG TAAAAGTAGA   241 GAAGGCTCAC GGACAGACGA TGAAGTTGTG CAGAGAGAGG AAGAAGCTAT TCAGTTGGAT   301 GGTTTAAATG CATCACAAAT AAGGGAACTT AGAGAAAAAT CGGAAAAGTT CGCCTTCCAA   361 GCTGAAGTTA ACAGGATGAT GAAACTTATC ATCAATTCTT TGTATAAAAA TAAAGAGATT   421 TTCCTGAGAG AACTGATTTC AAATGCTTCT GATGCTTTAG ACAAGATAAG GCTCATCTCA   481 CTAACTGATG AAAATGCACT TGCTGGAAAT GAGGAGTTGA CGGTCAAGAT TAAGTGTGAC   541 AGAGAGAAAA ACCTGCTGCA TGTCACAGAC ACGGGTGTAG GAATGACCAG AGAGGAGCTG   601 GTTAAAAACC TGGGCACCAT AGCCAAATCT GGAACCAGCG AGTTTCTAAA CAAAATGACA   661 GAAGCACAAG AAGACGGTCA GTCAACTTCC GAACTGATCG GCCAGTTTGG TGTCGGTTTT   721 TATTCTGCCT TCCTTGTAGC AGATAAGGTC ATTGTCACGT CGAAACACAA CAATGATACC   781 CAGCACATCT GGGAATCCGA CTCTAATGAA TTCTCTGTGA TTGCTGACCC AAGAGGAAAC   841 ACACTAGGTC GTGGAACAAC GATTACTCTT GTCTTAAAAG AAGAAGCATC TGATTACCTT   901 GAATTGGATA CAATTAAAAA ATCTCGTCAG AAAGTATTCT CAGTTTCATC AACTTTCCAT   961 CTATGTGTGG AGTAGCAAGA CTGAGACTGT GGAGGAGCCC TTTGGTAGAG AACGAAACCA  1021 GCACCAGAGA GATGGAAGCA GCCTGAATGA ATGGAGCCGG CAGTAGAGAG GAGGAGGAAG 1081 AGTAGAGGCC AAGACGAAGA //
Primer: mCherry-N_rev Sequence file: RDB15154_A7Flb.seq
>D04425A3_A7Fl_2_mCherry-N_R_B08_05_ABI24.ab1     1 TCCATACTCT TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCGACC    61 GGTGGATCCC GGGCCCGCGG CAATTCATCC TTCTCTGTAG GTTCCTTTTC TGTTTCCTGT   121 TCTTCTTCTT CTTCTTCTGC CCCTGCGTCT GTCTCTTCTT CATCCTGCTC GGAGTCATCT   181 GTGGTGTCTT CTGTGGTGTC TTCAGGCTCC TCCTCTGGCT CTTCCTCTAC CTGTGCTTCA   241 GGGTCAATGT TTAAGCTGAG GCGGAGCATC CTTTCTATCC TGTCTCCATA TGCCTTGGTG   301 TCGGGTAGAA GGTATCCTGA CCGAAGAGTT GCCGTTTCAA ACAAAACTAC AGCAAGATCC   361 ATGACGGTCT TGTCATCTTC ATCTTCCTTA ACCCTCCGCA GCATGTCCCT GATCAGTGGG   421 TGTCTAGGAT TAATTTCAAA CGTTTTCTTC TGACTGGCAT AGTAATTTGT AGAGATGTCC   481 TTGCCCGTCT GGTATGCTTG TGCCTTCATG ATCCTCTCCA TGTTTCCAGA CCATCCATAC   541 TGGCTGGCCA CAAGGGCACA GGGAGACTCT GTGAGGCGCT GGGACACCAC AGCCTTTTCG   601 ATCTTGTCCT TGAGGGCCTT ATCTTTCATC CAGTTGAGCA GAGGCTCAAA CTCCTTCTCT   661 GTTGCTTCCC GACTTTCTTT ACTTTTCTCA CTCTCATCAA ACTTCACTCC TTCTTTGGCA   721 ACATTCTGGA ACCGTTTCCC ATCAAACTCA GGAAGAGCCT GAATGCAGTA TTCATCCACG   781 GGCTCTGTGA GGTAAATAAC TTCATAGCCC TTCTTCAGAA GCCTCTCAAC AAACGGAGAA   841 GATTCAGCCT CTTTTCTGCT TGACCCAGCC ATGAAGTAGA TTTTGTCCTG TTTTTCCTTC   901 ATTCTTTCCA CATACTGGTC TAAACTAGTA ATGTCAGTTG AAATGGGTGA GAAGACTGGG   961 AAACCTAAGA AGTTTAGCAA AGCCGTGTTT CGATTTGAGT GATCTTTCAA ATCACACCCA  1021 AAGCTTTGAT ATTGGTGCCC AAAATTCTTT TCCAGGAAAG TTGTTCATTA TATTTTCTCA 1081 ATCAGTCGAA TCCATCCTTG GATCAATGGT CCAGAAGTTT TGGCGGGTC //
Primer: SV40polyA-R Sequence file: RDB15154_A7Flc.seq
>D04425A3_A7Fl_2_SV40polyA-R_C08_08_ABI24.ab1     1 AAAAAGGGGG AGTTTTTAAG CAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT    61 AGAGTCGCGG CCGCTAGAGC TCATCCTTCT TGTACAGCTC GTCCATGCCG CCGGTGGAGT   121 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAGGTGA   181 TGTCCAACTT GATGTTGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT   241 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA   301 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGAGGAGGCC TCCCAGCCCA   361 TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT   421 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT   481 CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT   541 CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA   601 GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG   661 TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG   721 AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT   781 CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC CCGCGGCAAT TCATCCTTCT   841 CTGTAGGTTC CTTTTCTGTT TCCTGTTCTT CTTCTTCTTC TTCTGCCCCT GCGTCTGTCT   901 CTTCTTCATC CTGCTCGGAG TCATCTGTGG TGTCTTCTGT GGTGTCTTCA GGCTCCTCCT   961 CTGGCTCTTT CCTCTACCTG TGCTTCAGAT CAATGTTTAA GCTGGAGCGG AAGCCATCCC  1021 TTTCTATCCT GTCTCCATAT GCCCTTTGAG TCGGGTAGAA AGTATCTTGA CGGAAGAGGT 1081 TGCCGGTTTC AAACCAGA //
Primer: SV40pro_F_V2 Sequence file: RDB15154_A7Fld.seq
>D04425A3_A7Fl_2_SV40pro_F_V2_D08_11_ABI24.ab1     1 GACCGGGCTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC    61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG   121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG   181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA   241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC   301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC   361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA   421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA   481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT   541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT   601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT   661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA   721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA   781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG   841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG   901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GGTTGGGGCT   961 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GGAATGATCC CTCCAGCGCG GGGATCTCAT  1021 TGCTGAAGTC TTCGCCACCC TAGGGAGCCT ACTGAACACG GAAGGAGACC ATTACTGGAA 1081 GGACTCGCGC TATGAACGGT CAATAT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl