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ENPL-mCherry-KDEL (#RDB15154)

Expression vector of rat Endoplasmin (ENPL), mCherry-ER retention signal (KDEL) tag.

Clone info. Expression vector of rat Endoplasmin (ENPL) with C-terminally tagged mCherry-ER retention signal (KDEL). cDNA fragment 125nt to 2536nt of NM_001012197.2 is cloned.
Comment Expression was confirmed by the depositor with confocal lase scanning microscopy. Known differences (at least): T to C substitution at 2251nt of NM_001012197.2 (Thr, Synonym).
Vector backbone pmCherry-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name rat Endoplasmin cDNA
Depositor|Developer Hamachi, Itaru |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Nat. Methods 13 (11): 931-937, 2016)
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RDB15154 ENPL-mCherry-KDEL DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The ENPL-mCherry-KDEL was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15154).

Reference section:

Miki, T., Awa, M., Nishikawa, Y., Kiyonaka, S., Wakabayashi, M., Ishihama, Y., Hamachi, I., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15154_A7Flp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB15154_A7Fla.seq check
>D04425A3_A7Fl_2_CMV-Forward_A08_02_ABI24.ab1
    1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTCGAGGCC
  121 ACCATGAGGG TCCTGTGGGT GCTGGGCCTC TGCTGCGTCC TGCTGACCTT CGGGTTTGTG
  181 AGAGCTGACG ATGAAGTCGA TGTGGATGGC ACGGTAGAAG AGGACCTGGG TAAAAGTAGA
  241 GAAGGCTCAC GGACAGACGA TGAAGTTGTG CAGAGAGAGG AAGAAGCTAT TCAGTTGGAT
  301 GGTTTAAATG CATCACAAAT AAGGGAACTT AGAGAAAAAT CGGAAAAGTT CGCCTTCCAA
  361 GCTGAAGTTA ACAGGATGAT GAAACTTATC ATCAATTCTT TGTATAAAAA TAAAGAGATT
  421 TTCCTGAGAG AACTGATTTC AAATGCTTCT GATGCTTTAG ACAAGATAAG GCTCATCTCA
  481 CTAACTGATG AAAATGCACT TGCTGGAAAT GAGGAGTTGA CGGTCAAGAT TAAGTGTGAC
  541 AGAGAGAAAA ACCTGCTGCA TGTCACAGAC ACGGGTGTAG GAATGACCAG AGAGGAGCTG
  601 GTTAAAAACC TGGGCACCAT AGCCAAATCT GGAACCAGCG AGTTTCTAAA CAAAATGACA
  661 GAAGCACAAG AAGACGGTCA GTCAACTTCC GAACTGATCG GCCAGTTTGG TGTCGGTTTT
  721 TATTCTGCCT TCCTTGTAGC AGATAAGGTC ATTGTCACGT CGAAACACAA CAATGATACC
  781 CAGCACATCT GGGAATCCGA CTCTAATGAA TTCTCTGTGA TTGCTGACCC AAGAGGAAAC
  841 ACACTAGGTC GTGGAACAAC GATTACTCTT GTCTTAAAAG AAGAAGCATC TGATTACCTT
  901 GAATTGGATA CAATTAAAAA ATCTCGTCAG AAAGTATTCT CAGTTTCATC AACTTTCCAT
  961 CTATGTGTGG AGTAGCAAGA CTGAGACTGT GGAGGAGCCC TTTGGTAGAG AACGAAACCA
 1021 GCACCAGAGA GATGGAAGCA GCCTGAATGA ATGGAGCCGG CAGTAGAGAG GAGGAGGAAG
1081 AGTAGAGGCC AAGACGAAGA
//
Primer: mCherry-N_rev
Sequence file: RDB15154_A7Flb.seq check
>D04425A3_A7Fl_2_mCherry-N_R_B08_05_ABI24.ab1
    1 TCCATACTCT TGATGATGGC CATGTTATCC TCCTCGCCCT TGCTCACCAT GGTGGCGACC
   61 GGTGGATCCC GGGCCCGCGG CAATTCATCC TTCTCTGTAG GTTCCTTTTC TGTTTCCTGT
  121 TCTTCTTCTT CTTCTTCTGC CCCTGCGTCT GTCTCTTCTT CATCCTGCTC GGAGTCATCT
  181 GTGGTGTCTT CTGTGGTGTC TTCAGGCTCC TCCTCTGGCT CTTCCTCTAC CTGTGCTTCA
  241 GGGTCAATGT TTAAGCTGAG GCGGAGCATC CTTTCTATCC TGTCTCCATA TGCCTTGGTG
  301 TCGGGTAGAA GGTATCCTGA CCGAAGAGTT GCCGTTTCAA ACAAAACTAC AGCAAGATCC
  361 ATGACGGTCT TGTCATCTTC ATCTTCCTTA ACCCTCCGCA GCATGTCCCT GATCAGTGGG
  421 TGTCTAGGAT TAATTTCAAA CGTTTTCTTC TGACTGGCAT AGTAATTTGT AGAGATGTCC
  481 TTGCCCGTCT GGTATGCTTG TGCCTTCATG ATCCTCTCCA TGTTTCCAGA CCATCCATAC
  541 TGGCTGGCCA CAAGGGCACA GGGAGACTCT GTGAGGCGCT GGGACACCAC AGCCTTTTCG
  601 ATCTTGTCCT TGAGGGCCTT ATCTTTCATC CAGTTGAGCA GAGGCTCAAA CTCCTTCTCT
  661 GTTGCTTCCC GACTTTCTTT ACTTTTCTCA CTCTCATCAA ACTTCACTCC TTCTTTGGCA
  721 ACATTCTGGA ACCGTTTCCC ATCAAACTCA GGAAGAGCCT GAATGCAGTA TTCATCCACG
  781 GGCTCTGTGA GGTAAATAAC TTCATAGCCC TTCTTCAGAA GCCTCTCAAC AAACGGAGAA
  841 GATTCAGCCT CTTTTCTGCT TGACCCAGCC ATGAAGTAGA TTTTGTCCTG TTTTTCCTTC
  901 ATTCTTTCCA CATACTGGTC TAAACTAGTA ATGTCAGTTG AAATGGGTGA GAAGACTGGG
  961 AAACCTAAGA AGTTTAGCAA AGCCGTGTTT CGATTTGAGT GATCTTTCAA ATCACACCCA
 1021 AAGCTTTGAT ATTGGTGCCC AAAATTCTTT TCCAGGAAAG TTGTTCATTA TATTTTCTCA
1081 ATCAGTCGAA TCCATCCTTG GATCAATGGT CCAGAAGTTT TGGCGGGTC
//
Primer: SV40polyA-R
Sequence file: RDB15154_A7Flc.seq check
>D04425A3_A7Fl_2_SV40polyA-R_C08_08_ABI24.ab1
    1 AAAAAGGGGG AGTTTTTAAG CAGTAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
   61 AGAGTCGCGG CCGCTAGAGC TCATCCTTCT TGTACAGCTC GTCCATGCCG CCGGTGGAGT
  121 GGCGGCCCTC GGCGCGTTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG TGGGAGGTGA
  181 TGTCCAACTT GATGTTGACG TTGTAGGCGC CGGGCAGCTG CACGGGCTTC TTGGCCTTGT
  241 AGGTGGTCTT GACCTCAGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC CTCTGCTTGA
  301 TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGAGGAGGCC TCCCAGCCCA
  361 TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC AGCTTCACCT
  421 TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC ACGCCGCCGT
  481 CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC TTCAAGTAGT
  541 CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACATGAAC TGAGGGGACA
  601 GGATGTCCCA GGCGAAGGGC AGGGGGCCAC CCTTGGTCAC CTTCAGCTTG GCGGTCTGGG
  661 TGCCCTCGTA GGGGCGGCCC TCGCCCTCGC CCTCGATCTC GAACTCGTGG CCGTTCACGG
  721 AGCCCTCCAT GTGCACCTTG AAGCGCATGA ACTCCTTGAT GATGGCCATG TTATCCTCCT
  781 CGCCCTTGCT CACCATGGTG GCGACCGGTG GATCCCGGGC CCGCGGCAAT TCATCCTTCT
  841 CTGTAGGTTC CTTTTCTGTT TCCTGTTCTT CTTCTTCTTC TTCTGCCCCT GCGTCTGTCT
  901 CTTCTTCATC CTGCTCGGAG TCATCTGTGG TGTCTTCTGT GGTGTCTTCA GGCTCCTCCT
  961 CTGGCTCTTT CCTCTACCTG TGCTTCAGAT CAATGTTTAA GCTGGAGCGG AAGCCATCCC
 1021 TTTCTATCCT GTCTCCATAT GCCCTTTGAG TCGGGTAGAA AGTATCTTGA CGGAAGAGGT
1081 TGCCGGTTTC AAACCAGA
//
Primer: SV40pro_F_V2
Sequence file: RDB15154_A7Fld.seq check
>D04425A3_A7Fl_2_SV40pro_F_V2_D08_11_ABI24.ab1
    1 GACCGGGCTT TTTTGGAGGC TAGGCTTTTG CAAGATCGAT CAAGAGACAG GATGAGGATC
   61 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
  121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
  181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
  241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
  301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
  361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
  421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
  481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
  541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT
  601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
  661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
  721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
  781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
  841 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
  901 CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GGTTGGGGCT
  961 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GGAATGATCC CTCCAGCGCG GGGATCTCAT
 1021 TGCTGAAGTC TTCGCCACCC TAGGGAGCCT ACTGAACACG GAAGGAGACC ATTACTGGAA
1081 GGACTCGCGC TATGAACGGT CAATAT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miki, T., A conditional proteomics approach to identify proteins involved in zinc homeostasis. Nat. Methods 13 (11): 931-937 (2016). PMID 27617391. [link to RRC of NBRP]