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pBSIISK-hFLEX

Plasmid clone of Cre recombinase mediated flip-excision (FLEX) switch.

Catalog number RDB15114
Resource name pBSIISK-hFLEX
Clone info. Plasmid clone of flip-excision (FLEX) switch, genetic tool for specific gene expression under Cre recombinase exposure.
Vector backbone pBSIISK (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Depositor|Developer Hioki, Hiroyuki |
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External Database
            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)
            Ordering Please visit Information of Request for Distribution.[link] 
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sohn, J. et al. PLoS One, 12 (1): e0169611, 2017)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB15114 pBSIISK-hFLEX DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15114zzk01.seq provided by the depositor

            Original reference

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15114_A7Fdp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: f1ori_R3
            Sequence file: RDB15114_A7Fda.seq check
            >D04569A8-2_A7Fd_f1ori_R3_D05_11_ABI24.ab1
                1 TCCATTCAGC TGCGCACTGT TGGGAGGGCG ATCGGTGCGG GCCTCTTCGC TATTACGCCA
               61 GCTGGCGAAA GGGGGATGTG CTGCAAGGCG ATTAAGTTGG GTAACGCCAG GGTTTTCCCA
              121 GTCACGACGT TGTAAAACGA CGGCCAGTGA GCGCGCGTAA TACGACTCAC TATAGGGCGA
              181 ATTGGGTACC TTCGAAGGAT CCCCATAACT TCGTATAAAG TATCCTATAC GAAGTTATAT
              241 CAAAATAGGA AGACCAAGTC TTCACCATCG ACCCGAATTG CCAAGCATCA CCATCGACCC
              301 ATAACTTCGT ATAGCATACA TTATACGAAG TTATCTGTGA GTGAATTCGT CGACGATATC
              361 CTGCAGATAA CTTCGTATAG GATACTTTAT ACGAAGTTAT CATTGGGATT CTTCCTATTT
              421 TGATCCAAGC ATCACCATCG ACCCTCTAGT CCAGATCTCA CCATCGACCC ATAACTTCGT
              481 ATAATGTATG CTATACGAAG TTATGTCCCT CGAAGAGGTT CACGCGTGCG GCCGCGAGCT
              541 CCAGCTTTTG TTCCCTTTAG TGAGGGTTAA TTGCGCGCTT GGCGTAATCA TGGTCATAGC
              601 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA
              661 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT
              721 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC
              781 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC
              841 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT
              901 TATCCACAGA ATCAGGGGAT AACGCAGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC
              961 CAGGAACCGT AAAAAGCCGC GTTGCTGGCG TTTTTCCATA GCTCCGCCCC CTGACGAGCA
             1021 TCACAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGGA CAGACTATAA AGAATACCAG
             1081 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC CTCCTCCTGT TCCGACCTTG CGCTTACGAT
             1141 ACTGGTCGGC GTTCCTCCCT CGAAGCGATG GCCGCTTTCT CTCTATAGAG CTTACTAACG
            1201 A
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Sohn, J., A Single Vector Platform for High-Level Gene Transduction of Central Neurons: Adeno-Associated Virus Vector Equipped with the Tet-Off System. PLoS One 12 (1): e0169611 (2017). PMID 28060929.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl