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pGEX-4T-3-RBD35

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), GST-tag.

Catalog number RDB15106
Resource name pGEX-4T-3-RBD35
Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal GST, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Rusc2 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Rusc2

          Reference sequence
            
           
          Sequence (full) RDB15106hts01.seqcheck assembled from experimentally sequenced data
          Test sheet RDB15106_B4Cdp1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)。
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15106 pGEX-4T-3-RBD35 DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pGEX-4T-3-RBD35 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15106).

          Reference section:

          Fukuda, M., Kobayashi, H., Ishibashi, K., Ohbayashi, N., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          References

          Original, user report and related articles

          original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]

          2024.03.29

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