Resource data sheet
DNA Bank Top

pGEX-4T-3-RBD35 (#RDB15106)

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), GST-tag.


Drawn by SnapGene® software
Sequence (full) RDB15106hts01.seq
Publication Fukuda, M., Cell Struct. Funct. 36 (2): 155-170 (2011). [link to RRC of NBRP]
Test sheet RDB15106_B4Cdp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal GST, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Rusc2 cDNA
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15106 pGEX-4T-3-RBD35 DNA solution

How to cite this biological resource

Materials & Methods section:

The pGEX-4T-3-RBD35 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15106).

Reference section:

Fukuda, M., Kobayashi, H., Ishibashi, K., Ohbayashi, N., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]