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pGEX-4T-3-RBD35

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), GST-tag.

Catalog number RDB15106
Resource name pGEX-4T-3-RBD35
Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal GST, tac promoter.
Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Rusc2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Other clones in our bank mouse Rusc2 (NCBI Gene 100213) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
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RDB15106 pGEX-4T-3-RBD35 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15106_A7F7p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2
Sequence file: RDB15106_A7F7a.seq check
>D04592B9_A7F7_1_pGEX-F2_D03_12_ABI24.ab1
    1 GCTACAGTTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC
   61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
  121 CCTCCAAAAT CGGATCTGGT TCCGCGTGGA TCTTCTCCCG ATGGCAACTC GGAGGCCATA
  181 TCCATCGACA TACTTCAGAA AAAAGGGCTG GTAAAAGCTG TCAACACCGC TGTGGACCTT
  241 ATTGTGGCCC ATTTTGGCAC GAGCCGGGAT CCTGGCGTGA AGGCAAAGCT GGGCAACAGT
  301 TCTGTGAGCC CCAATGTCGG CCACCTGGTT CTGAAGTACT TGTGCCCTGC AGTCCAGGCC
  361 GTGCTCGAGG ACGGACTCAA GGCCTTCGTA CTGGATGTGA TCATCGGCCA ACGTAAAAAC
  421 ATGCCTTGGA GTGTGGTCGA GGCTTCCACA CAGCTAGGCC CATCCACCAA GGTCTTGCAT
  481 GGCCTCTACA ACAAAGTCAG CCAGTTCCCA GAACTCACCA GTCACACCAT GCGCTTCAAC
  541 GCCTTCATCC TCGGCCTGCT CAACATCCGG TCTCTGGAGT TCTGGTTTAA TCACCTGTAT
  601 AACCATGAAG ATATCATCCA GACCCACTAC CAGCCCTGGG GCTTCCTGCG GGCCGCACAC
  661 ACGGTGTGCC CAGGCCTCTT CGAGGAGCTG CTGCTGTTGC TACAGCCCTT AGCCCTGCTA
  721 CCCTTCAGCC TGGACTTGCT GTTCCAGCAC CGGCTGCTGC AGAGCGGGCG GCAGCAGCGG
  781 CAGCACAAGG AACTGCTGCG TGTGTCCTAG AATCACTAGT GAATTCGCGG CCGCATCGTG
  841 ACTGACTGAC GATCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC
  901 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
  961 AGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA
 1021 TAGCGGAGTG TTATCTCCTG AGGACGAAAG GTCTCGTGAT ACGCTAATTT TATAGGTTAA
1081 TGTTCAATGA ATA
//
Primer: SV40pA3'element Reverse
Sequence file: RDB15106_A7F7b.seq check
>D04592B9_A7F7_1_SV40pA3'element Reverse_F03_18_ABI24.ab1
    1 GTGTCAGGAG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
   61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
  121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
  181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG
  241 TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT
  301 ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG
  361 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC
  421 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG
  481 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC
  541 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT
  601 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA
  661 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC
  721 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG
  781 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC
  841 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA
  901 GCGGGCCCAT TAAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC ATAAATATCT
  961 CACTCGCAAT CAAATTCAGC CGATAGCGGG ACGGGAAGGC GACTGGTAGT GCCATGTCCC
 1021 GGCTTTTCAA CAATCAATGC AATGCTGGAA TGAGGGCATC GATTCCAACT TGCCGATGGC
1081 CTTGGAATTG ACCAAACGGA T
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

2021.03.26

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