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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGEX-4T-3-RBD35

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), GST-tag.

Catalog number RDB15106
Resource name pGEX-4T-3-RBD35
Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal GST, tac promoter.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pGEX-4T-3 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse Rusc2 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rusc2
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)。
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          RDB15106 pGEX-4T-3-RBD35 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15106_A7F7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-F2
          Sequence file: RDB15106_A7F7a.seq check
          >D04592B9_A7F7_1_pGEX-F2_D03_12_ABI24.ab1
              1 GCTACAGTTT GTTTTAAAAC GTATTGAGCT ATCCCACAAA TTGATAAGTA CTTGAAATCC
             61 AGCAAGTATA TAGCATGGCC TTTGCAGGGC TGGCAAGCCA CGTTTGGTGG TGGCGACCAT
            121 CCTCCAAAAT CGGATCTGGT TCCGCGTGGA TCTTCTCCCG ATGGCAACTC GGAGGCCATA
            181 TCCATCGACA TACTTCAGAA AAAAGGGCTG GTAAAAGCTG TCAACACCGC TGTGGACCTT
            241 ATTGTGGCCC ATTTTGGCAC GAGCCGGGAT CCTGGCGTGA AGGCAAAGCT GGGCAACAGT
            301 TCTGTGAGCC CCAATGTCGG CCACCTGGTT CTGAAGTACT TGTGCCCTGC AGTCCAGGCC
            361 GTGCTCGAGG ACGGACTCAA GGCCTTCGTA CTGGATGTGA TCATCGGCCA ACGTAAAAAC
            421 ATGCCTTGGA GTGTGGTCGA GGCTTCCACA CAGCTAGGCC CATCCACCAA GGTCTTGCAT
            481 GGCCTCTACA ACAAAGTCAG CCAGTTCCCA GAACTCACCA GTCACACCAT GCGCTTCAAC
            541 GCCTTCATCC TCGGCCTGCT CAACATCCGG TCTCTGGAGT TCTGGTTTAA TCACCTGTAT
            601 AACCATGAAG ATATCATCCA GACCCACTAC CAGCCCTGGG GCTTCCTGCG GGCCGCACAC
            661 ACGGTGTGCC CAGGCCTCTT CGAGGAGCTG CTGCTGTTGC TACAGCCCTT AGCCCTGCTA
            721 CCCTTCAGCC TGGACTTGCT GTTCCAGCAC CGGCTGCTGC AGAGCGGGCG GCAGCAGCGG
            781 CAGCACAAGG AACTGCTGCG TGTGTCCTAG AATCACTAGT GAATTCGCGG CCGCATCGTG
            841 ACTGACTGAC GATCTGCCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC
            901 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
            961 AGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCGCAGCC ATGACCCAGT CACGTAGCGA
           1021 TAGCGGAGTG TTATCTCCTG AGGACGAAAG GTCTCGTGAT ACGCTAATTT TATAGGTTAA
          1081 TGTTCAATGA ATA
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB15106_A7F7b.seq check
          >D04592B9_A7F7_1_SV40pA3'element Reverse_F03_18_ABI24.ab1
              1 GTGTCAGGAG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
             61 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC
            121 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC
            181 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG
            241 TGCGGTATTT CACACCGCAT AAATTCCGAC ACCATCGAAT GGCGCAAAAC CTTTCGCGGT
            301 ATGGCATGAT AGCGCCCGGA AGAGAGTCAA TTCAGGGTGG TGAATGTGAA ACCAGTAACG
            361 TTATACGATG TCGCAGAGTA TGCCGGTGTC TCTTATCAGA CCGTTTCCCG CGTGGTGAAC
            421 CAGGCCAGCC ACGTTTCTGC GAAAACGCGG GAAAAAGTGG AAGCGGCGAT GGCGGAGCTG
            481 AATTACATTC CCAACCGCGT GGCACAACAA CTGGCGGGCA AACAGTCGTT GCTGATTGGC
            541 GTTGCCACCT CCAGTCTGGC CCTGCACGCG CCGTCGCAAA TTGTCGCGGC GATTAAATCT
            601 CGCGCCGATC AACTGGGTGC CAGCGTGGTG GTGTCGATGG TAGAACGAAG CGGCGTCGAA
            661 GCCTGTAAAG CGGCGGTGCA CAATCTTCTC GCGCAACGCG TCAGTGGGCT GATCATTAAC
            721 TATCCGCTGG ATGACCAGGA TGCCATTGCT GTGGAAGCTG CCTGCACTAA TGTTCCGGCG
            781 TTATTTCTTG ATGTCTCTGA CCAGACACCC ATCAACAGTA TTATTTTCTC CCATGAAGAC
            841 GGTACGCGAC TGGGCGTGGA GCATCTGGTC GCATTGGGTC ACCAGCAAAT CGCGCTGTTA
            901 GCGGGCCCAT TAAAGTTCTG TCTCGGCGCG TCTGCGTCTG GCTGGCTGGC ATAAATATCT
            961 CACTCGCAAT CAAATTCAGC CGATAGCGGG ACGGGAAGGC GACTGGTAGT GCCATGTCCC
           1021 GGCTTTTCAA CAATCAATGC AATGCTGGAA TGAGGGCATC GATTCCAACT TGCCGATGGC
          1081 CTTGGAATTG ACCAAACGGA T
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl