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pEGFP-C1-RBD35 (#RDB15105)

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), EGFP-tag.

Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope (Fig.3 of Fukuda, M., Cell Struct. Funct. 36, 155-170, 2011),
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rusc2 cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15105 pEGFP-C1-RBD35 Under QC test. Please contact us. DNA solution

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How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-RBD35 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15105).

Reference section:

Fukuda, M., Kobayashi, H., Ishibashi, K., Ohbayashi, N., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958. [link to RRC of NBRP]