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pEGFP-C1-RBD35

Expression vector of mouse rab-binding domain specific for Rab35 (RBD35), EGFP-tag.

Catalog number RDB15105
Resource name pEGFP-C1-RBD35
Clone info. Expression vector of mouse rab-binding domain specific for Rab35 (RBD35) [Rusc2 in NCBI], fused with N-terminal EGFP, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope (Fig.3 of Fukuda, M., Cell Struct. Funct. 36, 155-170, 2011),
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rusc2 cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rusc2 (NCBI Gene 100213) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2011) Cell Struct. Funct. 36, 155-170)
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Schedule A (GFP-A) [Link]
Remarks
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Distribution information (domestic users) [open/close]


Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB15105 pEGFP-C1-RBD35 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Fukuda, M., Genome-wide investigation of the Rab binding activity of RUN domains: development of a novel tool that specifically traps GTP-Rab35. Cell Struct. Funct. 36 (2): 155-170 (2011). PMID 21737958.

2019.07.17

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