Expression vector of mouse double C2, gamma (Doc2gamma), T7-tag.
Clone info. | Expression vector of mouse double C2, gamma (Doc2gamma), tagged with T7, EF1 promoter. |
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Comment | Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. |
Vector backbone | pEF-T7 (plasmid) |
Selectable markers | Amp^r |
Gene/insert name | mouse Doc2g cDNA |
Reference of insert sequence | AB046665 (DDBJ accession number) |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. (2001) J. Biol. Chem. 276, 24441-24444; Fukuda, M. and Mikoshiba, K. (2000) Biochem. Biophys. Res. Commun. 276, 626-632) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. (2001) J. Biol. Chem. 276, 24441-24444; Fukuda, M. and Mikoshiba, K. (2000) Biochem. Biophys. Res. Commun. 276, 626-632)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15102 | pEF-T7-mouse Doc2 gamma | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEF-T7-mouse Doc2 gamma was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15102). |
Reference section:
Fukuda, M., Saegusa, C., Kanno, E., Mikoshiba, K., The C2A domain of double C2 protein gamma contains a functional nuclear localization signal. J. Biol. Chem. 276 (27): 24441-24444 (2001). PMID 11371549. [link to RRC of NBRP] |
Fukuda, M., Mikoshiba, K., Doc2gamma, a third isoform of double C2 protein, lacking calcium-dependent phospholipid binding activity. Biochem. Biophys. Res. Commun. 276 (2): 626-632 (2000). PMID 11027523. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Fukuda, M., The C2A domain of double C2 protein gamma contains a functional nuclear localization signal. J. Biol. Chem. 276 (27): 24441-24444 (2001). PMID 11371549. [link to RRC of NBRP] |
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original | Fukuda, M., Doc2gamma, a third isoform of double C2 protein, lacking calcium-dependent phospholipid binding activity. Biochem. Biophys. Res. Commun. 276 (2): 626-632 (2000). PMID 11027523. [link to RRC of NBRP] |
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