Expression vector of a long form of mouse regulating synaptic membrane exocytosis protein 2 (Rim2 +44A), T7-tag.
Clone info. | Expression vector of a long form of mouse regulating synaptic membrane exocytosis protein 2 (Rim2 +44A) [Rims2 in NCBI], tagged with T7, EF1 promoter, see Fig2A of Fukuda, M. Genes Cells 9 (9): 831-842, 2004. |
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Comment | Expression was confirmed by the depositor with immunoblotting and immunoprecipitation. |
Vector backbone | pEF-T7 (plasmid) |
Selectable markers | Amp^r |
Gene/insert name | mouse Rims2 cDNA |
Reference of insert sequence | AB162901 (DDBJ accession number) |
Depositor|Developer | Fukuda, Mitsunori | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. (2004) Genes Cells 9, 831-842) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. (2004) Genes Cells 9, 831-842)。 MTAに書く付加的使用条件: pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。 |
Catalog # | Resource name | Availability | Shipping form | Fee (non-profit org.) |
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RDB15099 | pEF-T7-mouse Rim2(+44A) | Under QC test. Please contact us. | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pEF-T7-mouse Rim2(+44A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15099). |
Reference section:
Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.
Original, user report and related articles
original | Fukuda, M., Alternative splicing in the first alpha-helical region of the Rab-binding domain of Rim regulates Rab3A binding activity: is Rim a Rab3 effector protein during evolution? Genes Cells 9 (9): 831-842 (2004). PMID 15330860. [link to RRC of NBRP] |
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