RDB15094 - pmRFP-C1-mouse rabphilin

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pmRFP-C1-mouse rabphilin

Expression vector of mouse rabphilin, RFP-tag.


Catalog number	RDB15094
Resource name	pmRFP-C1-mouse rabphilin
Clone info.	Expression vector of mouse rabphilin [Rph3a in NCBI], fused with N-terminal RFP, CMV promoter. Expression was confirmed by the depositor with immunoprecipitation. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.5 of Tsuboi, T. J. Biol Chem., 280, 39253-39259, 2005).
Vector backbone	pmRFP-C1-gk (plasmid)
Selectable markers	Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	mouse Rph3a cDNA
Reference of nucleotide sequence	AB112924 (DDBJ accession number)
Depositor\|Developer	Fukuda, Mitsunori \|
Other clones in our bank	mouse Rph3a (NCBI Gene 19894) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)

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Remarks
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Distribution information (domestic users) [open/close]

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)
Ordering	Please visit Information of Request for Distribution.[link] UCSD's MTA is also required. Please visit Additional Forms section. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	UCSD's MTA is also required. Please visit Additional Forms section.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	別途、UCSDとのMTAも必要です。「申込みに必要な書式」をご覧ください。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15094	pmRFP-C1-mouse rabphilin	DNA solution

Ordering Information [in Japanese] [in English]
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References and tips

Electronic file

Original reference

original	Tsuboi, T., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731.

Further references such as user reports and related articles (go to bottom)

Featured content	Visualization of Organelles (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15094_A7F7p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, insert 5' (mRFP1-Gly linker-rabphilin) Sequence file: RDB15094_A7F7a.seq
>D04592A6_A7F7_2_CMV Forward_D06_12_ABI24.ab1 1 CGTCCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTATGGCCT 121 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA 181 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA 241 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC 301 CTCAGTTCCA GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT 361 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG 421 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA 481 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT 541 GGGAGGCCTC CACCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGA 601 TGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCCGA GGTCAAGACC ACCTACATGG 661 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAAGACCGA CATCAAGCTG GACATCACCT 721 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CGCCGAGGGC CGCCACTCCA 781 CCGGCGCCTC CGGTGGTTCC GGTGGTACCG GTGGATCCAT GACTGACACT GTGGTGAACC 841 GATGGATGTA CCCTGGTGAT GGCCCTCTGC AGTCAAATGA CAAGGAACAG CTGCAGGCAG 901 GATGGTCCGT CCATCCTGGA GCACAGACCG ACAGGCAGAG GAAGCAGGAA GAACTGACAG 961 ACGAGGAGAA GGAGATCATC AACCAGAGTG ATTTGCTCGG GCAGAGAAGA TGGGAAAGCC 1021 ATGGACAGAA CGCATTTGAG CCGCTTGCTT GCACGTTCTT GGAGACCATG AGGAGCATGT 1081 GGCCTGGCAG ATGGCGGTGA ACCGCC //
Primer: mRFP1-C_F Region: insert mid (mRFP1-Gly linker-rabphilin) Sequence file: RDB15094_A7F7b.seq
>D04592A6_A7F7_2_mRFP1-C_F_F11_06_ABI08.ab1 1 TGGCCCTCAG ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT 61 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCTCCGGTG GTTCCGGTGG 121 TACCGGTGGA TCCATGACTG ACACTGTGGT GAACCGATGG ATGTACCCTG GTGATGGCCC 181 TCTGCAGTCA AATGACAAGG AACAGCTGCA GGCAGGATGG TCCGTCCATC CTGGAGCACA 241 GACCGACAGG CAGAGGAAGC AGGAAGAACT GACAGACGAG GAGAAGGAGA TCATCAACAG 301 AGTGATTGCT CGGGCAGAGA AGATGGAAGC CATGGAACAG GAACGCATTG GGCGCCTGGT 361 GGACCGTCTG GAGACCATGA GGAAGAATGT GGCTGGAGAT GGCGTGAACC GCTGCATTCT 421 GTGTGGGGAA CAGCTGGGTA TGCTGGGCTC GGCCTGTGTC GTGTGTGAAG ACTGTAAGAA 481 GAATGTCTGC ACCAAGTGTG GGGTTGAGAC CTCCAACAAC CGTCCGCATC CGGTATGGCT 541 CTGCAAGATC TGCCTTGAGC AGAGAGAGGT CTGGAAGCGC TCAGGAGCAT GGTTCTTCAA 601 AGGTTTCCCC AAGCAGGTCC TTCCACAGCC CATGCCTATA AAGAAGACCA AGCCCCAGCA 661 GCCTGCTGGT GAACCGGCCA CCCAGGAGCA GCCTACACCT GAGTCCAGGC ATCCAGCCAG 721 GGCTCCAGCT CGAGGTGACA TGGAGGACAG GAGGCCCCCA GGGCAGAAGC CAGGCCCTGA 781 CCTCACCTCT GCTCCTGGGA GAGGAAGCCA TGGGCCTCCC ACGCGTAGGG CCTCTGAGGC 841 ACGGATGAGT ACAGCCGCCC GGGATTCTGA GGGCTGGGAC CATGCCCATG GTGGGGGTAC 901 TGGAGACACC AGCCGTAGCC CAGCAGTTTG AGGCGAGCTA AACTCAGTCC AGGCAGCCCG 961 CCCTGCCCCA GCCCCAGTGC CAAGCCCAGC ACCTCTCAGC CGGTGCAGCC AGGGCCCCCT 1021 GGGGGCAGCA GGCACTCTGG CCAGGGACGC TTTCCGGAGC AGAGCACAGA GCTCTTCAAG 1081 TGACCTGGCC TATTCCAGGG CCTGTCGCCC CAAGCCTGAA GGAAGAACGG ACCTTGCCGG 1141 GGGCCTTCAG CCAGGCGCCC GCCCACACTG TCAG //
Primer: SV40polyA-R Region: insert 3' (rabphilin) Sequence file: RDB15094_A7F7c.seq
>D04592A6_A7F7_2_SV40polyA-R_E06_15_ABI24.ab1 1 AAGAAGTGAG TTTTTAAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG 61 AGTCGCGGCC GCTTAAGGTC AGAGTAAATC TCTTTGTAAA TGTCTCCCCA CACCCACTAC 121 CCAACCTCTG GGGACCAGAG AGGGCTCACA TCCAGACCCT GAAGCCCAGA GATTCTTCTT 181 CTGTAGCAGG ACCTGAAGTG GGGGAGACAT GAAAGGTTCA TAGTTGGCAG GGGAGGCTCT 241 GAAAGGGGAG AGCGGCTGGG GCAGAGACAG GGGACACCAG GGCACCAGGT CCTAATCACT 301 GGACACGTGG TTCTCGTTCT GCAGTTGGTG CCAGCGCTCA ATCTTCTTGT CTTTGTTCTT 361 CAAACACTCA TACCAATGTT TCAAGCGCTC GCCTTTGGCC GAGATCCCCA GCTGGCAGCC 421 TCCGATGTAA TCATTAGACT TGCCAATGTC GTAGTCCCAC ACCGAGATAT CCAGGGACTT 481 TTTAGCCAGG TCGCTGTGTT TGATATCATA AAAGAACTCC TCGTTAAACT CGGGATTCAG 541 GGTCTTCTTT TTAATCTGAG TCTTGTGCTT GGCTTTCTTC CCCATGTCCG GTTTCAGCCA 601 GAGCTTGACA AAGGGGTCTG AGTAGCCGTT GGCATCCATG GCGGCCAGGT GCACACAGCG 661 GATGATTCCC ACAATGAGGC CGCCCTGCTG CGTGCTGTAC ATGAGGGACA CCAGGATCTT 721 GCCCCGTTCC TCTATATCGC CGATCCGCTC TACCTGCTCC TCCTCATAGA GAGCCATGCC 781 ACGGGCCGAC CCGGTGGTCC CTGCTCTCTT CATCGGGATC ACCCGCTCCA GGCAGATGTT 841 GAAGTTTTTC CTCTGGTTAG CCTTCAGCTT CTTGAGCGAG AACCTGGTCT CACCAATGAA 901 CTCGTTGTGG CCAAACTTGT CCTCGTCACA CACGGAGATC CTTAGTGTCT TCCCTCTGCA 961 TGTCCCTCCT CTGTAATGCC CATGATACTG CAGTGTCTCA TTTCCACACA GGTTTCGAGT 1021 GGTGGCGGCA CGTCTTTGTA CGAAGCCTGA TGGACTTGCT GTCTCAGCAG CAGATGAAGC 1081 CTTCACATAG GAATTCTTGC CCAG //
Primer: SV40pro_F_V2 Region: NeoR_KanR Sequence file: RDB15094_A7F7d.seq
>D04592A6_A7F7_2_SV40pro_F_V2_F06_18_ABI24.ab1 1 TACACGGCTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG 61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG 601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCCT TCTATGAAAG GTTGGGGCTT 961 CGGAATCGTT TTCCCGGGAC GCCCGGCTGG AATGATCCTC CAGCGCGGGG GATCTCATGG 1021 CTTGCAGGTT CTTCGCCCCA CCCTACGGGA AGCTACTGAA ACACGTTGGA GACCATTACT 1081 GCAGAACCCG CCGCTATGAA CGGGTCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsuboi, T., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731.
reference	Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887.

2020.11.04

GNP_filter3_RDBDEP_html_201104.pl