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pmRFP-C1-mouse rabphilin (#RDB15094)

Expression vector of mouse rabphilin, RFP-tag.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Tsuboi, T., J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse rabphilin [Rph3a in NCBI], fused with N-terminal RFP, CMV promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.5 of Tsuboi, T. J. Biol Chem., 280, 39253-39259, 2005).
Vector backbone pmRFP-C1-gk (plasmid)
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Growth conditions LB+Kanamycin, 37oC
Gene/insert name mouse Rph3a cDNA
Reference of insert sequence AB112924 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee
RDB15094 pmRFP-C1-mouse rabphilin DNA solution

How to cite this biological resource

Materials & Methods section:

The pmRFP-C1-mouse rabphilin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15094).

Reference section:

Tsuboi, T., Fukuda, M., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tsuboi, T., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731. [PubMed] [Article] [RRC of NBRP]
reference Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887. [PubMed] [RRC of NBRP]