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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmRFP-C1-mouse rabphilin (#RDB15094)

Expression vector of mouse rabphilin, RFP-tag.

Clone info. Expression vector of mouse rabphilin [Rph3a in NCBI], fused with N-terminal RFP, CMV promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.5 of Tsuboi, T. J. Biol Chem., 280, 39253-39259, 2005).
Vector backbone pmRFP-C1-gk (plasmid)
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Rph3a cDNA
Reference of insert sequence AB112924 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2005) J. Biol. Chem. 280, 39253-39259; Fukuda, M. et al. (1994) 269, 29206-29211)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15094 pmRFP-C1-mouse rabphilin DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmRFP-C1-mouse rabphilin was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15094).

Reference section:

Tsuboi, T., Fukuda, M., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15094_A7F7p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5' (mRFP1-Gly linker-rabphilin)
Sequence file: RDB15094_A7F7a.seq check
>D04592A6_A7F7_2_CMV Forward_D06_12_ABI24.ab1
    1 CGTCCCTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTATGGCCT
  121 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA
  181 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA
  241 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC
  301 CTCAGTTCCA GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT
  361 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
  421 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA
  481 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT
  541 GGGAGGCCTC CACCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGA
  601 TGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCCGA GGTCAAGACC ACCTACATGG
  661 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAAGACCGA CATCAAGCTG GACATCACCT
  721 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CGCCGAGGGC CGCCACTCCA
  781 CCGGCGCCTC CGGTGGTTCC GGTGGTACCG GTGGATCCAT GACTGACACT GTGGTGAACC
  841 GATGGATGTA CCCTGGTGAT GGCCCTCTGC AGTCAAATGA CAAGGAACAG CTGCAGGCAG
  901 GATGGTCCGT CCATCCTGGA GCACAGACCG ACAGGCAGAG GAAGCAGGAA GAACTGACAG
  961 ACGAGGAGAA GGAGATCATC AACCAGAGTG ATTTGCTCGG GCAGAGAAGA TGGGAAAGCC
 1021 ATGGACAGAA CGCATTTGAG CCGCTTGCTT GCACGTTCTT GGAGACCATG AGGAGCATGT
1081 GGCCTGGCAG ATGGCGGTGA ACCGCC
//
Primer: mRFP1-C_F (Pr0502)
Region: insert mid (mRFP1-Gly linker-rabphilin)
Sequence file: RDB15094_A7F7b.seq check
>D04592A6_A7F7_2_mRFP1-C_F_F11_06_ABI08.ab1
    1 TGGCCCTCAG ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT
   61 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCTCCGGTG GTTCCGGTGG
  121 TACCGGTGGA TCCATGACTG ACACTGTGGT GAACCGATGG ATGTACCCTG GTGATGGCCC
  181 TCTGCAGTCA AATGACAAGG AACAGCTGCA GGCAGGATGG TCCGTCCATC CTGGAGCACA
  241 GACCGACAGG CAGAGGAAGC AGGAAGAACT GACAGACGAG GAGAAGGAGA TCATCAACAG
  301 AGTGATTGCT CGGGCAGAGA AGATGGAAGC CATGGAACAG GAACGCATTG GGCGCCTGGT
  361 GGACCGTCTG GAGACCATGA GGAAGAATGT GGCTGGAGAT GGCGTGAACC GCTGCATTCT
  421 GTGTGGGGAA CAGCTGGGTA TGCTGGGCTC GGCCTGTGTC GTGTGTGAAG ACTGTAAGAA
  481 GAATGTCTGC ACCAAGTGTG GGGTTGAGAC CTCCAACAAC CGTCCGCATC CGGTATGGCT
  541 CTGCAAGATC TGCCTTGAGC AGAGAGAGGT CTGGAAGCGC TCAGGAGCAT GGTTCTTCAA
  601 AGGTTTCCCC AAGCAGGTCC TTCCACAGCC CATGCCTATA AAGAAGACCA AGCCCCAGCA
  661 GCCTGCTGGT GAACCGGCCA CCCAGGAGCA GCCTACACCT GAGTCCAGGC ATCCAGCCAG
  721 GGCTCCAGCT CGAGGTGACA TGGAGGACAG GAGGCCCCCA GGGCAGAAGC CAGGCCCTGA
  781 CCTCACCTCT GCTCCTGGGA GAGGAAGCCA TGGGCCTCCC ACGCGTAGGG CCTCTGAGGC
  841 ACGGATGAGT ACAGCCGCCC GGGATTCTGA GGGCTGGGAC CATGCCCATG GTGGGGGTAC
  901 TGGAGACACC AGCCGTAGCC CAGCAGTTTG AGGCGAGCTA AACTCAGTCC AGGCAGCCCG
  961 CCCTGCCCCA GCCCCAGTGC CAAGCCCAGC ACCTCTCAGC CGGTGCAGCC AGGGCCCCCT
 1021 GGGGGCAGCA GGCACTCTGG CCAGGGACGC TTTCCGGAGC AGAGCACAGA GCTCTTCAAG
 1081 TGACCTGGCC TATTCCAGGG CCTGTCGCCC CAAGCCTGAA GGAAGAACGG ACCTTGCCGG
1141 GGGCCTTCAG CCAGGCGCCC GCCCACACTG TCAG
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3' (rabphilin)
Sequence file: RDB15094_A7F7c.seq check
>D04592A6_A7F7_2_SV40polyA-R_E06_15_ABI24.ab1
    1 AAGAAGTGAG TTTTTAAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG
   61 AGTCGCGGCC GCTTAAGGTC AGAGTAAATC TCTTTGTAAA TGTCTCCCCA CACCCACTAC
  121 CCAACCTCTG GGGACCAGAG AGGGCTCACA TCCAGACCCT GAAGCCCAGA GATTCTTCTT
  181 CTGTAGCAGG ACCTGAAGTG GGGGAGACAT GAAAGGTTCA TAGTTGGCAG GGGAGGCTCT
  241 GAAAGGGGAG AGCGGCTGGG GCAGAGACAG GGGACACCAG GGCACCAGGT CCTAATCACT
  301 GGACACGTGG TTCTCGTTCT GCAGTTGGTG CCAGCGCTCA ATCTTCTTGT CTTTGTTCTT
  361 CAAACACTCA TACCAATGTT TCAAGCGCTC GCCTTTGGCC GAGATCCCCA GCTGGCAGCC
  421 TCCGATGTAA TCATTAGACT TGCCAATGTC GTAGTCCCAC ACCGAGATAT CCAGGGACTT
  481 TTTAGCCAGG TCGCTGTGTT TGATATCATA AAAGAACTCC TCGTTAAACT CGGGATTCAG
  541 GGTCTTCTTT TTAATCTGAG TCTTGTGCTT GGCTTTCTTC CCCATGTCCG GTTTCAGCCA
  601 GAGCTTGACA AAGGGGTCTG AGTAGCCGTT GGCATCCATG GCGGCCAGGT GCACACAGCG
  661 GATGATTCCC ACAATGAGGC CGCCCTGCTG CGTGCTGTAC ATGAGGGACA CCAGGATCTT
  721 GCCCCGTTCC TCTATATCGC CGATCCGCTC TACCTGCTCC TCCTCATAGA GAGCCATGCC
  781 ACGGGCCGAC CCGGTGGTCC CTGCTCTCTT CATCGGGATC ACCCGCTCCA GGCAGATGTT
  841 GAAGTTTTTC CTCTGGTTAG CCTTCAGCTT CTTGAGCGAG AACCTGGTCT CACCAATGAA
  901 CTCGTTGTGG CCAAACTTGT CCTCGTCACA CACGGAGATC CTTAGTGTCT TCCCTCTGCA
  961 TGTCCCTCCT CTGTAATGCC CATGATACTG CAGTGTCTCA TTTCCACACA GGTTTCGAGT
 1021 GGTGGCGGCA CGTCTTTGTA CGAAGCCTGA TGGACTTGCT GTCTCAGCAG CAGATGAAGC
1081 CTTCACATAG GAATTCTTGC CCAG
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15094_A7F7d.seq check
>D04592A6_A7F7_2_SV40pro_F_V2_F06_18_ABI24.ab1
    1 TACACGGCTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCCT TCTATGAAAG GTTGGGGCTT
  961 CGGAATCGTT TTCCCGGGAC GCCCGGCTGG AATGATCCTC CAGCGCGGGG GATCTCATGG
 1021 CTTGCAGGTT CTTCGCCCCA CCCTACGGGA AGCTACTGAA ACACGTTGGA GACCATTACT
1081 GCAGAACCCG CCGCTATGAA CGGGTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., The C2B domain of rabphilin directly interacts with SNAP-25 and regulates the docking step of dense core vesicle exocytosis in PC12 cells. J. Biol. Chem. 280 (47): 39253-39259 (2005). PMID 16203731. [link to RRC of NBRP]
reference Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887. [link to RRC of NBRP]