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pmRFP-C1-mouse Slp5

Expression vector of mouse Synaptotagmin-like protein 5 (Slp5), RFP-tag.

Catalog number RDB15093
Resource name pmRFP-C1-mouse Slp5
Clone info. Expression vector of mouse Synaptotagmin-like protein 5 (Slp5) [Sytl5 in NCBI], fused with N-terminal RFP, CMV promoter.
Expression was confirmed by the depositor with immunoprecipitation and immunoblotting. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.1 of Tsuboi, T. Mol Biol. Cell, 17, 2101-2112, 2006).
Vector backbone pmRFP-C1-gk (plasmid)
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Sytl5 cDNA
Reference of nucleotide sequence AB098160 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Sytl5 (NCBI Gene 236643) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)
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RDB15093 pmRFP-C1-mouse Slp5 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Tsuboi, T., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Tsuboi, T., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396.
reference Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.

2019.07.17

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