RDB15092 - pmRFP-C1-mouse Slp4-a

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pmRFP-C1-mouse Slp4-a

Expression vector of mouse Synaptotagmin-like protein 4 (Slp4), RFP-tag.

Catalog number	RDB15092
Resource name	pmRFP-C1-mouse Slp4-a
Clone info.	Expression vector of mouse Synaptotagmin-like protein 4 (Slp4) [Sytl4 in NCBI], fused with N-terminal RFP, CMV promoter.
Comment	Expression was confirmed by the depositor with immunoprecipitation and immunoblotting. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.1 of Tsuboi, T. Mol Biol. Cell, 17, 2101-2112, 2006). This clone has a nucleotide sequence corresponding to 186 to 2207 nt of AB025258.1. Known difference (at least): A to C substitution (Arg, synonym) at 1953 nt of AB025258.1.
Vector backbone	pmRFP-C1-gk (plasmid)
Selectable markers	Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name	mouse Sytl4 (Slp4) cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

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mouse Sytl4 (Slp4)

External Database
mouse Sytl4 (Slp4)

Reference sequence
AB025258

Sequence (partial)	RDB15092pka03.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15092	pmRFP-C1-mouse Slp4-a	DNA solution

Please review the QC test results indicated by check

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How to cite this biological resource

Materials & Methods section:

The pmRFP-C1-mouse Slp4-a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15092).

Reference section:

Tsuboi, T., Fukuda, M., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15092_A7F7p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' (mRFP1, Gly-linker) Sequence file: RDB15092_A7F7a.seq
>D04592A4_A7F7_1_CMV Forward_D05_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTATGGCCT 121 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA 181 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA 241 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC 301 CTCAGTTCCA GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT 361 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG 421 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA 481 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT 541 GGGAGGCCTC CACCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGA 601 TGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCCGA GGTCAAGACC ACCTACATGG 661 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAAGACCGA CATCAAGCTG GACATCACCT 721 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CGCCGAGGGC CGCCACTCCA 781 CCGGCGCCTC CGGNGGTTCC GGTGGTACCG GNGGNTCCNT GTCNGANATA CTAGACNTCT 841 CTTTTCTGTC NNANNTGGAA AGGGNTTTGA TNCTCGGNGT TCTGCNNANA NNTGAAGAAN 901 TCANNAAAGC AGANGANAAA ANGNNTCNNN NANNNNNNNN NGANCTNNNN NNNNNN //
Primer: SV40polyA-R (Pr0109) Region: insert 3' (mSlp4-a) Sequence file: RDB15092_A7F7b.seq
>D04592A4_A7F7_1_SV40polyA-R_E05_14_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNA NNNNNNCTCT ACNNATGTGG TATGGCTGAT TATGATCTAG 61 AGTCGCGGCC GCTATGGTCG ACCTGCAGGC GGCCGCGAAT TCACTAGTGA TTGCGAATTC 121 ATACACCCAG CTTCTGCTTG ACCATTGAAG AACGGAGCTG CAGAGTTCCT TCTGCCCAAG 181 ACCCTGGGTA CTGTCGCATC TTCTGCCACA AGCTTACTTC TTCACCAGTC GAATCCATCC 241 AGTCCACCAC TTCGCCATTA CTGATCCCAG TGCCAACACC CAGTCTGACC CCTCCCAGGA 301 AGTCATTGCT GGCCAGGGGC TCCCGGTCCC ATACAGTCAG TTCCAGGCAC ATGTGTTGTA 361 GATCTTCCAG CCGCACACCA TTGTAGACAA ATGTATGGTT GTAGTGGGGA CTCAGAGTCT 421 TCTTCATCAC AGGAGTTTTA CGCTTACTGG CCTTGTTCCT CATGGGAAGG AGGTATCCCT 481 TGACAAAGCT GTCTGAAGTC CCTCCTGATT TGGCAGCCGT CAGGTTCTTG GCTTCTTTGA 541 TCCACACCTG GAGTTCTCCC CCTTCCCCAC CTTTACTCTT TTTCCGGTCA CCTCCAACAG 601 GGAGTTTGGA GGCTGGGATG TATTTCAATG AAACCACCAA CTCGCCTTTG TGTGCTGGCA 661 AACCAGGCGA GGACTCAGTA CTGATCTTTC CATGTAAAGG GAGGCAGTGA TCCAGCTTCT 721 TATCCAGCTT CCAAGAGTCC ATGTGGACCT CCGCCTCTCC AAGGAAAGTG TTTCTGCCAA 781 AACGACCATG NTGCCNAACG GAAAACTGCA AAGTCCTCTG ANCCAGAAGA NATTCCNAAA 841 TCTCGNACCG AAAGGTCTCA TCATATAGNG GANTGATGGN GNCCCGCTTG ATGNTGGTTT 901 TTCNTTTTNC CTTNGCGGGN NTNNNCNNNN AGAANANNNN NCTTCNCNNN NGGGNNANNA 961 NNNNNNNNNN //
Primer: mRFP1-C_F (Pr0502) Region: insert mid (mRFP1, Gly-linker, mSlp4-a) Sequence file: RDB15092_A7F7c.seq
>D04592A4_A7F7_1_mRFP1-C_F_D11_04_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNCN NGCTGGNNNT CNCCTCCNNC NACGAGGNNT ACACCATCGT 61 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCTCCGGTG GTTCCGGTGG 121 TACCGGTGGA TCCATGTCGG AGATACTAGA CCTCTCTTTT CTGTCCGAGA TGGAAAGGGA 181 TTTGATCCTC GGTGTTCTGC AGAGAGATGA AGAACTCAGA AAAGCAGATG AGAAAAGGAT 241 TCGGCGACTG AAGAATGAGC TCCTGGAGAT CAAAAGAAAA GGGGCCAAAA GGGGCAGCCA 301 ACACTATAGT GATCGAACCT GTGCCAGGTG CCAGGAGGGC CTGGGCCGCT TAATTCCCAA 361 AAGCAGCACT TGTGTGGGCT GCAATCATTT AGTGTGCCGA GAATGCCGAG TTCTGGAAAG 421 CAATGGCTCT TGGAGGTGCA AGGTGTGCTC GAAGGAGATA GAGCTGAAGA AAGCAACTGG 481 AGATTGGTTT TATGACCAGA AAGTAAATCG CTTTGATTAC CGAACAGGCA GCGAGATAAT 541 CAGGATGTCC CTGCGCCAGA AGCCTGCAGT GAATAAAAGA GAGACAGCGG GGCAGTCCCT 601 CCTTCAGCAG ACACAGATGG GTGATATCTG GCCAGGAAGA AGGATCATTC AAGAACAACA 661 ACAGAGGGAA CAAAGTGTGC TGTTTGAAGT GCCNAAGACC AGAAGTGGAA AGAGTGCACT 721 GGANGCTGAG AGCGAGAGTC TGGATAGCTA CACTGCTGAC TCAGACAGCA CCTCCAGGAG 781 AGANTCTCTG GATAAATCTG GACTCTTCCC AGAATGGAAG ANNANGTCCG CTCCCNAATC 841 TCAAGTTGAA AAGGAAATTC CACCNNGGAA NCNAAATGCA GTCTGTGGTG ATGAGGGTGA 901 NNTGGNANTC NAGAAGAACA CCAAAAAANT CCTGANGCCT TCNNANNACN NTNANNCTGN 961 NNTAGANCNT NNNCCNANNA NANGTNGNCN NNNNANNNNG NATCNTNNGG ANNNAGANNN 1021 NNNNNNN //
Primer: SV40pro_F_V2 (Pr0154) Region: NeoR_KanR Sequence file: RDB15092_A7F7d.seq
>D04592A4_A7F7_1_SV40pro_F_V2_F05_17_ABI24.ab1 1 NNNNNNNNNN NNNNNNCNNN GCTTTTGNNN NNNCGATCNA GANANAGGNN GAGGNTCGTT 61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC 601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA 721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG 781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCN NGATTCGCAN CGCATCGCCT TCTATCGCCT 841 TCTTGACGAN TTCTTCTGAN CGGGANTCTG GGGTTCGAAA TGANCNANCN AGCGACGCCN 901 NACCNGCCNT CNCGANNNTN CNANTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tsuboi, T., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396.
reference	Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134.

2023.05.01

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