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pmRFP-C1-mouse Slp4-a (#RDB15092)

Expression vector of mouse Synaptotagmin-like protein 4 (Slp4), RFP-tag.

Clone info. Expression vector of mouse Synaptotagmin-like protein 4 (Slp4) [Sytl4 in NCBI], fused with N-terminal RFP, CMV promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblotting. Mainly localizes to the dense-core vesicle in PC12 cell (Fig.1 of Tsuboi, T. Mol Biol. Cell, 17, 2101-2112, 2006). This clone has a nucleotide sequence corresponding to 186 to 2207 nt of AB025258.1. Known difference (at least): A to C substitution (Arg, synonym) at 1953 nt of AB025258.1.
Vector backbone pmRFP-C1-gk (plasmid)
Selectable markers Km^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Sytl4 (Slp4) cDNA
Depositor|Developer Fukuda, Mitsunori |
 
Sequence (partial) RDB15092pka03.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)
Remarks
This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Tsuboi, T. and Fukuda, M. (2006) Mol. Biol. Cell 17, 2101-2112; Fukuda, M. (2003) J. Biol. Chem. 278, 15390-15396)。
備考
Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15092 pmRFP-C1-mouse Slp4-a DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pmRFP-C1-mouse Slp4-a was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15092).

Reference section:

Tsuboi, T., Fukuda, M., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15092_A7F7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, insert 5' (mRFP1, Gly-linker)
Sequence file: RDB15092_A7F7a.seq check
>D04592A4_A7F7_1_CMV Forward_D05_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGCGCTAC CGGACTCAGA TCTATGGCCT
  121 CCTCCGAGGA CGTCATCAAG GAGTTCATGC GCTTCAAGGT GCGCATGGAG GGCTCCGTGA
  181 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA
  241 CCGCCAAGCT GAAGGTGACC AAGGGCGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC
  301 CTCAGTTCCA GTACGGCTCC AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT
  361 TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
  421 GCGTGGTGAC CGTGACCCAG GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA
  481 AGCTGCGCGG CACCAACTTC CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT
  541 GGGAGGCCTC CACCGAGCGG ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGA
  601 TGAGGCTGAA GCTGAAGGAC GGCGGCCACT ACGACGCCGA GGTCAAGACC ACCTACATGG
  661 CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT ACAAGACCGA CATCAAGCTG GACATCACCT
  721 CCCACAACGA GGACTACACC ATCGTGGAAC AGTACGAGCG CGCCGAGGGC CGCCACTCCA
  781 CCGGCGCCTC CGGNGGTTCC GGTGGTACCG GNGGNTCCNT GTCNGANATA CTAGACNTCT
  841 CTTTTCTGTC NNANNTGGAA AGGGNTTTGA TNCTCGGNGT TCTGCNNANA NNTGAAGAAN
901 TCANNAAAGC AGANGANAAA ANGNNTCNNN NANNNNNNNN NGANCTNNNN NNNNNN
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3' (mSlp4-a)
Sequence file: RDB15092_A7F7b.seq check
>D04592A4_A7F7_1_SV40polyA-R_E05_14_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNNNNCTCT ACNNATGTGG TATGGCTGAT TATGATCTAG
   61 AGTCGCGGCC GCTATGGTCG ACCTGCAGGC GGCCGCGAAT TCACTAGTGA TTGCGAATTC
  121 ATACACCCAG CTTCTGCTTG ACCATTGAAG AACGGAGCTG CAGAGTTCCT TCTGCCCAAG
  181 ACCCTGGGTA CTGTCGCATC TTCTGCCACA AGCTTACTTC TTCACCAGTC GAATCCATCC
  241 AGTCCACCAC TTCGCCATTA CTGATCCCAG TGCCAACACC CAGTCTGACC CCTCCCAGGA
  301 AGTCATTGCT GGCCAGGGGC TCCCGGTCCC ATACAGTCAG TTCCAGGCAC ATGTGTTGTA
  361 GATCTTCCAG CCGCACACCA TTGTAGACAA ATGTATGGTT GTAGTGGGGA CTCAGAGTCT
  421 TCTTCATCAC AGGAGTTTTA CGCTTACTGG CCTTGTTCCT CATGGGAAGG AGGTATCCCT
  481 TGACAAAGCT GTCTGAAGTC CCTCCTGATT TGGCAGCCGT CAGGTTCTTG GCTTCTTTGA
  541 TCCACACCTG GAGTTCTCCC CCTTCCCCAC CTTTACTCTT TTTCCGGTCA CCTCCAACAG
  601 GGAGTTTGGA GGCTGGGATG TATTTCAATG AAACCACCAA CTCGCCTTTG TGTGCTGGCA
  661 AACCAGGCGA GGACTCAGTA CTGATCTTTC CATGTAAAGG GAGGCAGTGA TCCAGCTTCT
  721 TATCCAGCTT CCAAGAGTCC ATGTGGACCT CCGCCTCTCC AAGGAAAGTG TTTCTGCCAA
  781 AACGACCATG NTGCCNAACG GAAAACTGCA AAGTCCTCTG ANCCAGAAGA NATTCCNAAA
  841 TCTCGNACCG AAAGGTCTCA TCATATAGNG GANTGATGGN GNCCCGCTTG ATGNTGGTTT
  901 TTCNTTTTNC CTTNGCGGGN NTNNNCNNNN AGAANANNNN NCTTCNCNNN NGGGNNANNA
961 NNNNNNNNNN
//
Primer: mRFP1-C_F (Pr0502)
Region: insert mid (mRFP1, Gly-linker, mSlp4-a)
Sequence file: RDB15092_A7F7c.seq check
>D04592A4_A7F7_1_mRFP1-C_F_D11_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCN NGCTGGNNNT CNCCTCCNNC NACGAGGNNT ACACCATCGT
   61 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCTCCGGTG GTTCCGGTGG
  121 TACCGGTGGA TCCATGTCGG AGATACTAGA CCTCTCTTTT CTGTCCGAGA TGGAAAGGGA
  181 TTTGATCCTC GGTGTTCTGC AGAGAGATGA AGAACTCAGA AAAGCAGATG AGAAAAGGAT
  241 TCGGCGACTG AAGAATGAGC TCCTGGAGAT CAAAAGAAAA GGGGCCAAAA GGGGCAGCCA
  301 ACACTATAGT GATCGAACCT GTGCCAGGTG CCAGGAGGGC CTGGGCCGCT TAATTCCCAA
  361 AAGCAGCACT TGTGTGGGCT GCAATCATTT AGTGTGCCGA GAATGCCGAG TTCTGGAAAG
  421 CAATGGCTCT TGGAGGTGCA AGGTGTGCTC GAAGGAGATA GAGCTGAAGA AAGCAACTGG
  481 AGATTGGTTT TATGACCAGA AAGTAAATCG CTTTGATTAC CGAACAGGCA GCGAGATAAT
  541 CAGGATGTCC CTGCGCCAGA AGCCTGCAGT GAATAAAAGA GAGACAGCGG GGCAGTCCCT
  601 CCTTCAGCAG ACACAGATGG GTGATATCTG GCCAGGAAGA AGGATCATTC AAGAACAACA
  661 ACAGAGGGAA CAAAGTGTGC TGTTTGAAGT GCCNAAGACC AGAAGTGGAA AGAGTGCACT
  721 GGANGCTGAG AGCGAGAGTC TGGATAGCTA CACTGCTGAC TCAGACAGCA CCTCCAGGAG
  781 AGANTCTCTG GATAAATCTG GACTCTTCCC AGAATGGAAG ANNANGTCCG CTCCCNAATC
  841 TCAAGTTGAA AAGGAAATTC CACCNNGGAA NCNAAATGCA GTCTGTGGTG ATGAGGGTGA
  901 NNTGGNANTC NAGAAGAACA CCAAAAAANT CCTGANGCCT TCNNANNACN NTNANNCTGN
  961 NNTAGANCNT NNNCCNANNA NANGTNGNCN NNNNANNNNG NATCNTNNGG ANNNAGANNN
1021 NNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB15092_A7F7d.seq check
>D04592A4_A7F7_1_SV40pro_F_V2_F05_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNCNNN GCTTTTGNNN NNNCGATCNA GANANAGGNN GAGGNTCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ANCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGANCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCN NGATTCGCAN CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAN TTCTTCTGAN CGGGANTCTG GGGTTCGAAA TGANCNANCN AGCGACGCCN
901 NACCNGCCNT CNCGANNNTN CNANTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsuboi, T., The Slp4-a linker domain controls exocytosis through interaction with Munc18-1.syntaxin-1a complex. Mol. Biol. Cell 17 (5): 2101-2112 (2006). PMID 16481396. [link to RRC of NBRP]
reference Fukuda, M., Slp4-a/granuphilin-a inhibits dense-core vesicle exocytosis through interaction with the GDP-bound form of Rab27A in PC12 cells. J. Biol. Chem., 278, 15390-15396 (2003). PMID 12590134. [link to RRC of NBRP]