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pcDNA5-GAPDH-HaloTag

Expression vector for the visualization of chaperone-mediated autophagy (CMA).

Catalog number RDB15088
Resource name pcDNA5-GAPDH-HaloTag
Alternative name GAPDH-HT/pcDNA5/FRT
Clone info. Expression vector of human glyceraldehyde 3-phosphate dehydrogenase (GAPDH), tagged with C-terminal HaloTag, for genomic integration. Translocation of the GAPDH-HT labeled with an appropriate fluorescent dye as a substrate of chaperone-mediated autophagy (CMA) from cytosol to lysosome can be detected. Refer Fig. 1 of Seki, T., et al., PLoS ONE 7 (2), e31232, 2012.
Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA5/FRT (Plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r (E. coli), Hyg^r (mammalian cells).
Gene/insert name Human GAPDH cDNA
Depositor Seki, Takahiro |
 
Other clones in our bank human GAPDH (NCBI Gene 2597) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Sato, M. et al. Genes Cells 21 (8): 861-873, 2016).
(Japanese text [open/close])
Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15088 pcDNA5-GAPDH-HaloTag DNA solution

Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0071e (English text)
Featured content Dnaconda's recommendation EXR0071j (Japanese text)
Featured content Autophagy (English text)

References

original Sato, M., Fluorescent-based evaluation of chaperone-mediated autophagy and microautophagy activities in cultured cells. Genes Cells 21 (8): 861-873 (2016). PMID 27377049.
original Seki,T., Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron. PLoS One 7 (2): e31232 (2012). PMID 22363588.

2017.10.19

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