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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK229.AAV-EF1alpha-EGFP-WPRE (Supernova) (#RDB15061)

Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Supernova experiments.

Alternative name pK229
Clone info. Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Superniva experiments. Please refer Fig. 8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAAV-EF1α-DIO-EGFP (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
 
Sequence (full) RDB15061zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

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RDB15061 pK229.AAV-EF1alpha-EGFP-WPRE (Supernova) DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK229.AAV-EF1alpha-EGFP-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15061).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15061_B2Abp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 (Pr0041)
Region: EGFP 5'
Sequence file: RDB15061_B2Aba.seq check
>15061_15061_B2Ab_2_pAxEF_F1_E03_15_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGTCGTGAG GTACCGGATC NGCTGAATTC GCCNCCATGG
   61 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  121 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  181 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  241 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  301 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  361 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  421 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  481 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  541 TCNAGGTGAA CTTCNAGATC CGCCNCNACA TCGAGGACNG CAGCGTGCAG CTCGCCNACC
  601 NCTACCNGCA GAACACCCCC NTCGGCNANN GCCCCNNGCT GCTGCCCNAN NNCCNCTACC
  661 TGANCNCCNN GTNCGNCCTG ANNNAANANC CNNNNNANAA NCNNNNTNNC NNNNNNCNNN
721 NGNNNTNNNN GNCNNNNNNN NNN
//
Primer: WPRE_R (Pr0053)
Region: WPRE, EGFP 3'
Sequence file: RDB15061_B2Abb.seq check
>15061_15061_B2Ab_2_WPRE_R_F03_18_ABI24.ab1
    1 NNNNNNNNNN NCTTNNNNNN NNTTGTAATC NNGAGGTTGA TTATCGATAA GCTTGATAGC
   61 TGGATCCTTA CTTGTACAGC TCGTCCATGC CGAGAGTGAT CCCGGCGGCG GTCACGAACT
  121 CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT GCTCAGGGCG GACTGGGTGC
  181 TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC GATGGGGGTG TTCTGCTGGT
  241 AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG GCGGATCTTG AAGTTCACCT
  301 TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT GTGGCTGTTG TAGTTGTACT
  361 CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCTCGATGC
  421 GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TTGCCGTCGT
  481 CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT
  541 CGTGCTGCTT CNTGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCNTAN GTCNNGGTGG
  601 TCNCGANGGT GGGCNNGGGN NCNGGCNGCT TGCCNGNGGN GCNNANGNAC TTNNNNNCNN
661 CNNGNCGNNN NGGNNTCNNC NTCNNNNCNN NNNNNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: EF1 alpha pro, AAV ITR 1 3'
Sequence file: RDB15061_B2Abc.seq check
>15061_15061_B2Ab_2_EF1a-N_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG GGCGANGTGC GCTCTGCCCN CTGACGGGCA CCGGANCCAA
   61 TTCCCACTCC TTTCAAGACC TAGAAGGTCC ATTAGCTGCA AAGATTCCTC TCTGTTTAAA
  121 ACTTTATCCA TCTTTGCAAA GCTTACGCGT GCGGCCGCAG GAACCCCTAG TGATGGAGTT
  181 GGCCACTCCC TCTCTGCGCG CTCGNTCGNN NANNGNNGNC GGNCNACNNN NNGTCGCCCN
241 ACNCCCNGNN NGNNNNNNNN NN
//
Primer: hGH-pA_MF (Pr1113)
Region: hGH pA, AAV ITR 2 5'
Sequence file: RDB15061_B2Abd.seq check
>15061_15061_B2Ab_2_hGH-pA_MF_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNTNN NGNGNNCTAC CNNCCTTGGC CTCCCAAATT GCTGGGATTA
   61 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TGTAGGTAAC CACGTGCGGA
121 CCGAGCGGCC GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]