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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pK229.AAV-EF1alpha-EGFP-WPRE (Supernova)

Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Supernova experiments.

Catalog number RDB15061
Resource name pK229.AAV-EF1alpha-EGFP-WPRE (Supernova)
Alternative name pK229
Clone info. Adeno-associated virus (AAV)-based Supernova series. This clone will be used as a control in AAV-based Superniva experiments. Please refer Fig. 8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pAAV-EF1α-DIO-EGFP (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

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            RDB15061 pK229.AAV-EF1alpha-EGFP-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15061zzt01.seq compiled by us

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15061_A7Enp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pAxEF F1
            Sequence file: RDB15061_A7Ena.seq check
            >D04276C1_A7En_1_pAxEF F1_F11_17_ABI24.ab1
                1 AATTTTTTTC ATCATTTCAG GTGTCGTGAG GTACCGGATC AGCTGAATTC GCCACCATGG
               61 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
              121 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
              181 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
              241 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
              301 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
              361 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
              421 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
              481 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
              541 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
              601 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
              661 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
              721 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAGGAT
              781 CCAGCTATCA AGCTTATCGA TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT
              841 GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG
              901 TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTT
              961 GCTGTCTCTT TATGAGGAGT TGTGGGCCCG TTTGTCAGCA ACGTGGCGTA TGTGCACTGT
             1021 GTTTGCTGAC GCAACCCCCC ACTGGGTTGG GGGCATTGGC AACACTGTTC AGCTTCCGTT
            1081 TCCAGTACTT CGCTTTCCCC CCTTCCTATT GACCACGGGG CGGGAAC
            //
            Primer: EF1a-N_R
            Sequence file: RDB15061_A7Enb.seq check
            >D04276C1_A7En_1_EF1a-N_R_E11_14_ABI24.ab1
                1 CAGTCTCGGG GATGTGGGCG ATGTGCGCTC TGCCCACTGA CGGGCACCGG AGCCAATTCC
               61 CACTCCTTTC AAGACCTAGA AGGTCCATTA GCTGCAAAGA TTCCTCTCTG TTTAAAACTT
              121 TATCCATCTT TGCAAAGCTT ACGCGTGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC
              181 ACTCCCTCTC TGCGCGCTCG CTCGCTCAAT GAGGCCGGAA AAAAAGGTGT CGGGACGCCC
              241 GGGGGGCCGT CTGCGATGGG GGGGGTCTGC TGGGGGTGGT CGGAGAGCTG CACCCTGCCG
              301 TCCTCCATGT TGTGGCAGAT CTTGAACTTC ATCTAGATGC CGGTCTTCTG CTTGTCGGCC
              361 AAGATATAGA CGTTGTGGCT GTTGTAGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG
              421 TCTTCCTTGA AGTCAATGCC CTTCAGCTCG ATGAGGTTCA CCTGGGTGTC TCCCTCAAAC
              481 TTCACCTCGG CGCGGGTCTT GTATTTACCG TCATACTTGA AGAAGATGGT ACGCTCCTGG
              541 ACGTAGCCTT CAGGCATGGA GGAGATCAAG AAATCGTCCT GCTTCATGAA TCCGGAGGTT
            601 CAGCTGAATC CCTTCACGTC GTAGGACAGG
            //
            Primer: f1ori_R3
            Sequence file: RDB15061_A7Enc.seq check
            >D04276C1_A7En_1_f1ori_R3_D11_11_ABI24.ab1
                1 GGCAGGCTCT GCGTATGCGG TGTGAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT
               61 CAGGCGCCCC TGCAGGCAGC TGCGCGCTCG CTCGCTCACT GAGGCCGCCC GGGGTAAGCC
              121 CGGGCGTCGG GCAACCTTGA TCTCGGCGGC GGTCACGAAC TCGCGCGCGA CCAGGTGATC
              181 GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG
              241 CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC
              301 GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT
              361 GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAATTGTAC TCCAGCTTGT GCCCCAGGAT
              421 GTTGCCGTTT TTTCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC ATGGTGTCGC
            481 T
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl