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pK170.AAV-TRE-Cre-WPRE (Supernova)

Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.

Catalog number RDB15050
Resource name pK170.AAV-TRE-Cre-WPRE (Supernova)
Alternative name pK170
Clone info. Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. This Cre expression vector will be used with pK168 (RDB15049). Please refer Fig.8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK169 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。
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            RDB15050 pK170.AAV-TRE-Cre-WPRE (Supernova) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence (full) RDB15050zzk01.seq provided by the depositor

            Original reference

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB15050_A7Enp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Cre-N_R
            Sequence file: RDB15050_A7Ena.seq check
            >D04276A8_A7En_1_Cre-N_R_A07_01_ABI08.ab1
                1 TGAAGGCTCA GAAACGCCTG GCGATCCCTG AACATGTCCA TCAGGTTCTT GCGAACCTCA
               61 TCACTCGTTG CATCGACCGG TAATGCAGGC AAATTTTGGT GTACGGTCAG TAAATTCGAA
              121 ACCTTTCTCT TCTTCTTTGG GCCCATGGTC GACAGTGTAG CGAGAATTCT CCAGGCGATC
              181 TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC ACCGTACACG CCTACCTCGA
              241 CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG
              301 GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA AACTCGACAT ACGTTCTCTA
              361 TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC
              421 ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG TTCTCTATCA CTGATAGGGA
              481 GTAAACTCGA GTGAAGACGA AAGGGCCTCG TGATACGCCT GGCGCGCCAC GCGTGCGGCC
            541 GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTC
            //
            Primer: RRE_rev
            Sequence file: RDB15050_A7Enb.seq check
            >D04276A8_A7En_2_PRE_rev_D10_10_ABI24.ab1
                1 CGGAATCACA TAGCGTAAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
               61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGTCGACG CTAGCGGATC TGATATCCCT
              121 AATCGCCATC TTCCAGCAGG CGCACCATTG CCCCTGTTTC ACTATCCAGG TTACGGATAT
              181 AGTTCATGAC AATATTTACA TTGGTCCAGC CACCAGCTTG CATGATCTCC GGTATTGAAA
              241 CTCCAGCGCG GGCCATATCT CGCGCGGCTC CGACACGGGC ACTGTGTCCA GACCAGGCCA
              301 GGTATCTCTG ACCAGAGTCA TCCTTAGCGC CGTAAATCAA TCGATGAGTT GCTTCAAAAA
              361 TCCCTTCCAG GGCGCGAGTT GATAGCTGGC TGGTGGCAGA TGGCGCGGCA ACACCATTTT
              421 TTCTGACCCG GCAAAACAGG TAGTTATTCG GATCATCAGC TACACCAGAG ACGGAAATCC
              481 ATCGCTCGAC CAGTTTAGTT ACCCCCAGGC TAAGTGCCTT CTCTACACCT GCGGTGCTAA
              541 CCAGCGTTTT CGTTCTGCCA ATATGGATTA ACATTCTCCC ACCGTCAGTA CGTGAGATAT
              601 CTTTAACCCT GATCCTGGCA ATTTCGGCTA TACGTAACAG GGTGTTATAA GCAATCCCCA
              661 GAAATGCCAG ATTACGTATA TCCTGGCAGC GATCGCTATT TTCCATGAGT GAACGAACCT
              721 GGTCGAAATC AGTGCGTTCG AACGCTAGAG CCTGTTTTGC ACGTTCACCG GCATCAACGT
              781 TTTCTTTTCG GATCCGCCGC ATAACCAGTG AAACAGCATT GCTGTGGACT TGGTCGTGGC
              841 AGCCCGGACC GACGATGAAG CATGTTTAGC TGGCCCAAAT GTTGCTGGAT AGTTTTTTAC
              901 TGCCAGACCG CGCGCCTGAA GATATAGAAG ATAATCGCGA ACATCTTCAG GTTCTGCGGG
              961 AAACCATTTC CGGGTTATTC AACTTGCACC CATGCCGCCC ACGACCGGGC AACGGGACAG
             1021 AAAGCATTTT CCAGGTATGC TCAGAAAAAC GCCCTGATGG AATCCTGACA TGTCCATCAA
            1081 GTTCTGCGAT CTTCATTCAC TCGATTGCCA TCGGACCGGA TAATGGCCAA GGCCA
            //
            Primer: f1ori_R3
            Sequence file: RDB15050_A7Enc.seq check
            >D04276A8_A7En_2_f1ori_R3_C10_07_ABI24.ab1
                1 TCCATGCTCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
               61 TCAGGCGCCC CTGCAGGCAG CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC
              121 CCGGGCGTCG GCCGACCTTT GGCCGCCCGG CCTCCCTGTC GAGCGTGCGC GCAGCTGCTT
              181 GCAGGGGCGA CGTATTTACA TTGGTCCAGC CACCAGCTTG CATGATCTCC GGTATTGAAA
              241 CTCCGCGCGG GCCATATCTC GCGCGGCTCC GACACGGGCA CTGTGTCCAG ACCAGGCCAG
              301 GTATCTCTGA CCAGAGTCAT CCTTAGCGCC GTAAATCAAT CGATGAGTTG CTTCAAAAAT
              361 CCCTTCCAGG GCGCGAGTTG ATAGCTGGCT GGTGGCAGAT GGCGCGGCAA CACCATTTTT
              421 TCTGATTCTG CAAAACATGT AGGTATTCGG ATCATCGGCT ACACCAAAAA CGGAAATCCA
              481 TCGCTCGACC AGTTTAATTA CCCCCAGGCT AAGTGCCTTC TCTACACCTG CGGTGCTGAC
            541 CAACGTTTTC GTTCTGCCAA TATGGATTTA GCATTCTCCC GTGGTCAGTA CTTG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045.

            2022.05.17

            GNP_filter3_RDBDEP_html_220516.pl