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pK170.AAV-TRE-Cre-WPRE (Supernova) (#RDB15050)

Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase.

Alternative name pK170
Clone info. Adeno-associated virus (AAV)-based Supernova series, a versatile vector system for single-cell labeling relies on low tetracycline response (TRE) leakage in vivo. Expression vector of TRE-Cre recombinase. This Cre expression vector will be used with pK168 (RDB15049). Please refer Fig.8 of Luo, W. et al. Sci. Rep., 6:35747, 2016.
Comment Expression was confirmed by the depositor with immunohistochemistry.
Vector backbone pK169 (plasmid)
Selectable markers Amp^r (E. coli).
Depositor|Developer Iwasato, Takuji |
 
Sequence (full) RDB15050zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Luo, W. et al. Sci. Rep., 6:35747, 2016).
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提供依頼書  [依頼書の記入例 ]
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手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Luo, W. et al. Sci. Rep., 6:35747, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15050 pK170.AAV-TRE-Cre-WPRE (Supernova) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pK170.AAV-TRE-Cre-WPRE (Supernova) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15050).

Reference section:

Luo, W., Mizuno, H., Iwata, R., Nakazawa, S., Yasuda, K., Itohara, S., Iwasato, T., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15050_A7Enp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Cre-N_R
Sequence file: RDB15050_A7Ena.seq check
>D04276A8_A7En_1_Cre-N_R_A07_01_ABI08.ab1
    1 TGAAGGCTCA GAAACGCCTG GCGATCCCTG AACATGTCCA TCAGGTTCTT GCGAACCTCA
   61 TCACTCGTTG CATCGACCGG TAATGCAGGC AAATTTTGGT GTACGGTCAG TAAATTCGAA
  121 ACCTTTCTCT TCTTCTTTGG GCCCATGGTC GACAGTGTAG CGAGAATTCT CCAGGCGATC
  181 TGACGGTTCA CTAAACGAGC TCTGCTTATA TAGGCCTCCC ACCGTACACG CCTACCTCGA
  241 CATACGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA CGTTCTCTAT CACTGATAGG
  301 GATAAACTCG ACATACGTTC TCTATCACTG ATAGGGAGTA AACTCGACAT ACGTTCTCTA
  361 TCACTGATAG GGAGTAAACT CGACATACGT TCTCTATCAC TGATAGGGAG TAAACTCGAC
  421 ATCGTTCTCT ATCACTGATA GGGAGTAAAC TCGACATACG TTCTCTATCA CTGATAGGGA
  481 GTAAACTCGA GTGAAGACGA AAGGGCCTCG TGATACGCCT GGCGCGCCAC GCGTGCGGCC
541 GCAGGAACCC CTAGTGATGG AGTTGGCCAC TCCCTCTCTG CGCGCTC
//
Primer: RRE_rev
Sequence file: RDB15050_A7Enb.seq check
>D04276A8_A7En_2_PRE_rev_D10_10_ABI24.ab1
    1 CGGAATCACA TAGCGTAAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
   61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGTCGACG CTAGCGGATC TGATATCCCT
  121 AATCGCCATC TTCCAGCAGG CGCACCATTG CCCCTGTTTC ACTATCCAGG TTACGGATAT
  181 AGTTCATGAC AATATTTACA TTGGTCCAGC CACCAGCTTG CATGATCTCC GGTATTGAAA
  241 CTCCAGCGCG GGCCATATCT CGCGCGGCTC CGACACGGGC ACTGTGTCCA GACCAGGCCA
  301 GGTATCTCTG ACCAGAGTCA TCCTTAGCGC CGTAAATCAA TCGATGAGTT GCTTCAAAAA
  361 TCCCTTCCAG GGCGCGAGTT GATAGCTGGC TGGTGGCAGA TGGCGCGGCA ACACCATTTT
  421 TTCTGACCCG GCAAAACAGG TAGTTATTCG GATCATCAGC TACACCAGAG ACGGAAATCC
  481 ATCGCTCGAC CAGTTTAGTT ACCCCCAGGC TAAGTGCCTT CTCTACACCT GCGGTGCTAA
  541 CCAGCGTTTT CGTTCTGCCA ATATGGATTA ACATTCTCCC ACCGTCAGTA CGTGAGATAT
  601 CTTTAACCCT GATCCTGGCA ATTTCGGCTA TACGTAACAG GGTGTTATAA GCAATCCCCA
  661 GAAATGCCAG ATTACGTATA TCCTGGCAGC GATCGCTATT TTCCATGAGT GAACGAACCT
  721 GGTCGAAATC AGTGCGTTCG AACGCTAGAG CCTGTTTTGC ACGTTCACCG GCATCAACGT
  781 TTTCTTTTCG GATCCGCCGC ATAACCAGTG AAACAGCATT GCTGTGGACT TGGTCGTGGC
  841 AGCCCGGACC GACGATGAAG CATGTTTAGC TGGCCCAAAT GTTGCTGGAT AGTTTTTTAC
  901 TGCCAGACCG CGCGCCTGAA GATATAGAAG ATAATCGCGA ACATCTTCAG GTTCTGCGGG
  961 AAACCATTTC CGGGTTATTC AACTTGCACC CATGCCGCCC ACGACCGGGC AACGGGACAG
 1021 AAAGCATTTT CCAGGTATGC TCAGAAAAAC GCCCTGATGG AATCCTGACA TGTCCATCAA
1081 GTTCTGCGAT CTTCATTCAC TCGATTGCCA TCGGACCGGA TAATGGCCAA GGCCA
//
Primer: f1ori_R3
Sequence file: RDB15050_A7Enc.seq check
>D04276A8_A7En_2_f1ori_R3_C10_07_ABI24.ab1
    1 TCCATGCTCT TGCGTATGCG GTGTGAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA
   61 TCAGGCGCCC CTGCAGGCAG CTGCGCGCTC GCTCGCTCAC TGAGGCCGCC CGGGCAAAGC
  121 CCGGGCGTCG GCCGACCTTT GGCCGCCCGG CCTCCCTGTC GAGCGTGCGC GCAGCTGCTT
  181 GCAGGGGCGA CGTATTTACA TTGGTCCAGC CACCAGCTTG CATGATCTCC GGTATTGAAA
  241 CTCCGCGCGG GCCATATCTC GCGCGGCTCC GACACGGGCA CTGTGTCCAG ACCAGGCCAG
  301 GTATCTCTGA CCAGAGTCAT CCTTAGCGCC GTAAATCAAT CGATGAGTTG CTTCAAAAAT
  361 CCCTTCCAGG GCGCGAGTTG ATAGCTGGCT GGTGGCAGAT GGCGCGGCAA CACCATTTTT
  421 TCTGATTCTG CAAAACATGT AGGTATTCGG ATCATCGGCT ACACCAAAAA CGGAAATCCA
  481 TCGCTCGACC AGTTTAATTA CCCCCAGGCT AAGTGCCTTC TCTACACCTG CGGTGCTGAC
541 CAACGTTTTC GTTCTGCCAA TATGGATTTA GCATTCTCCC GTGGTCAGTA CTTG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Luo, W., Supernova: A Versatile Vector System for Single-Cell Labeling and Gene Function Studies in vivo. Sci. Rep. 6: 35747 (2016). PMID 27775045. [link to RRC of NBRP]