Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmStr-C1-mouse Rab11A-S25N

Expression vector of mouse constitutively negative (CN) mutant Rab11A (S25N), fused with N-terminal mStr, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15032_A7Egp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mStr-C_F Region: mStr 3', insert (S25N) Sequence file: RDB15032_A7Ega.seq
>D04556G8_A7Eg_2_mStr-C_F_H02_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNT CGNGGNNNTG TACGAACGCG CCGAGGGCCG CCACTCCNCC    61 GGCGGCATGG ACGAGCTGTA CAAGTCCGGA CTCAGATCCA TGGGCACCCG CGACGACGAG   121 TACGACTACC TCTTTAAAGT TGTCCTTATT GGAGATTCTG GTGTTGGAAA GAATAACCTC   181 CTGTCTCGAT TTACTCGAAA TGAGTTTAAT CTGGAAAGCA AGAGTACCAT TGGAGTAGAG   241 TTTGCAACAA GAAGCATCCA GGTTGATGGG AAAACAATAA AGGCACAGAT ATGGGACACA   301 GCAGGGCAGG AGCGGTACAG GGCTATAACG TCTGCATACT ATCGTGGAGC AGTAGGTGCC   361 TTATTGGTTT ATGACATTGC TAAGCATCTC ACATATGAAA ATGTAGAGCG ATGGCTGAAA   421 GAACTGAGAG ATCATGCTGA TAGTAACATT GTTATCATGC TTGTGGGCAA TAAGAGTGAT   481 TTACGTCATC TCAGGGCAGT TCCTACAGAT GAAGCAAGAG CTTTTGCAGA GAAGAATGGT   541 TTGTCATTCA TTGAGACATC TGCTCTAGAT TCTACAAATG TTGAAGCTGC TTTTCAGACA   601 ATTCTAACAG AGATATACCG CATTGTTTCT CAGAAGCAAA TGTCAGACAG ACGTGAAAAT   661 GACATGTCTC CAAGCAACAA TGTGGTTCCT ATTCATGTTC CGCCCACCAC TGAGAACAAG   721 CCAAAGGTGC AGTGCTGTCA GAACATCTAA AATCACTAGT GAATTCTGCA GTCGACGGTA   781 CCGCGGGCCC GGGATCCACC GGATCTAGAT AACTGATCAT AATCAGCCAT ACCACATTTG   841 TAGAGGTTTT ACTTGCTTTN AAAAACCTCN NACACNTCCC CNTGANNCTG AAACATAAAA   901 TGAATGCNAT TGNTGTTGTN NACTNGTTTA NNGNAGCTNA NNANGGTTAN NAATNAAGCN   961 ATAGCATCNN NAANTTCACN AATAAAGCAT TTTTTTTTCN NNGNATNNNA NTNNNNNNTN 1021 GNNCNNANNN NNNNNNN //
Primer: SV40polyA-R2 Region: insert 3' Sequence file: RDB15032_A7Egb.seq
>D04556G8_A7Eg_2_SV40polyA_R2_H03_24_ABI24.ab1     1 NNNNNNNNCN NNANNNANAN GTTAACAACA ACNATTGCAT TNNTTTTATG TTTCNGGTTC    61 AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG   121 ATTATGATCA GTTATCTAGA TCCGGTGGAT CCCGGGCCCG CGGTACCGTC GACTGCAGAA   181 TTCACTAGTG ATTTTAGATG TTCTGACAGC ACTGCACCTT TGGCTTGTTC TCAGTGGTGG   241 GCGGAACATG AATAGGAACC ACATTGTTGC TTGGAGACAT GTCATTTTCA CGTCTGTCTG   301 ACATTTGCTT CTGAGAAACA ATGCGGTATA TCTCTGTTAG AATTGTCTGA AAAGCAGCTT   361 CAACATTTGT AGAATCTAGA GCAGATGTCT CAATGAATGA CAAACCATTC TTCTCTGCAA   421 AAGCTCTTGC TTCATCTGTA GGAACTGCCC TGAGATGACG TAAATCACTC TTATTGCCCA   481 CAAGCATGAT AACAATGTTA CTATCAGCAT GATCTCTCAG TTCTTTCAGC CATCGCTCTA   541 CATTTTCATA TGTGAGATGC TTAGCAATGT CATAAACCAA TAAGGCACCT ACTGCTCCAC   601 GATAGTATGC AGACGTTATA GCCCTGTACC GCTCCTGCCC TGCTGTGTCC CATATCTGTG   661 CCTTTATTGT TTTCCCATCA ACCTGGATGC TTCTTGTTGC AAACTCTACT CCAATGGTAC   721 TCTTGCTTTC CAGATTAAAC TCATTTCGAG TAAATCGAGA CAGGANGTTA TTCTTTCNNA   781 CACCAGAATC TCCAATAANG ACAACTTTAA AGANGTAGTC GTACTCGTCG TCGCGGGTGN   841 CCNTGGATCT GANNNCGGAC TTGTACAGCT CGNCCATGNC GCCNGNGGAG TGNCGGCCCN   901 CGGCGCGTTC NNACAGTTCC NCGATGGNGN ANNCCNCNTT GNGGGANGNG ANGTCNNNNN 961 TGNTGCNNAN NNNNNNNNN //
Primer: CMV_Forward Region: CMV pro, mStr 5' Sequence file: RDB15032_A7Egc.seq
>D04556G8_A7Eg_2_CMV_Forward_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNNNNNGGG CGGTANGCGT GTACGGNGGG NGGTCTATAT    61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG   121 AGCAAGGGCG AGGAGAATAA CATGGCCATC ATCAAGGAGT TCATGCGCTT CAAGGTGCGC   181 ATGGAGGGCT CCGTGAACGG CCACGAGTTC GAGATCGAGG GCGAGGGCGA GGGCCGCCCC   241 TACGAGGGCA CCCAGACCGC CAAGCTGAAG GTGACCAAGG GTGGCCCCCT GCCCTTCGCC   301 TGGGACATCC TAACCCCCAA CTTCACCTAC GGCTCCAAGG CCTACGTGAA GCACCCCGCC   361 GACATCCCCG ACTACTTGAA GCTGTCCTTC CCCGAGGGCT TCAAGTGGGA GCGCGTGATG   421 AACTTCGAGG ACGGCGGCGT GGTGACCGTG ACCCAGGACT CCTCCCTGCA GGACGGCGAG   481 TTCATCTACA AGGTGAAGCT GCGCGGCACC AACTTCCCCT CCGACGGCCC CGTAATGCAG   541 AAGAAGACCA TGGGCTGGGA GGCCTCCTCC GAGCGGATGT ACCCCGAGGA CGGCGCCCTG   601 AAGGGCGAGA TCAAGATGAG GCTGAAGCTG AAGGACGGCG GCCACTACGA CGCTGAGGTC   661 AAGACCACCT ACAAGGCCAA GAAGCCCGTG CAGCTGCCCG GCGCCTACAT CGTCGGCATC   721 AAGTTGGACA TCACCTCCCA CAACGAGGAC TACACCATCG TGGAACTGTA CGAACGCGCC   781 GANGGCCGCC ACTCCACCGG CGGCATGGNC GANCTGTACA AGTCCGGNNT CAGATNCATG   841 GGNACCCGCG ACNACNANTA CGACTACCTC TTNNAAGTTG NNCTTNTNGG ANATTCNGGT   901 GTNNGAAAGA ATANCCTNCN NNCTCNATTT ANNNNAANTN ANNTNNANNN NNGNNANNNN 961 NNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB15032_A7Egd.seq
>D04556G8_A7Eg_2_SV40pro_F_V2_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNTTTTGN NNNNNCGATC NNGAGANAGG ATGAGGATCG    61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG   121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG   181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT   241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA   301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG   361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT   421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA   481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG   541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG   601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG   661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT   721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC   781 CGCTTCCTCG TGCTTTACNG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC   841 CTTCTTGACG AGTTCTTCTG ANCNGGACTC TGGGGTTCGA AATGACCGAN CAAGCGACGC   901 CNNNCCTGCC NTCNCGAGAT TTCNANTCCN CCGCCGNCNT TCTATGNNNN NTNNGGNNTC 961 NGNANTNNTT TNNCNNGGNN NNCNNGNNGG ATGATNCNTC NNNNNNNGGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl