Please review the QC test results indicated by icon below as well as clone information before placing your order.

pmStr-C1-mouse Rab11A-Q70L

Expression vector of mouse constitutively active (CA) mutant Rab11A (Q70L), fused with N-terminal mStr, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15031_A7Egp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: mStr-C_F Region: mStr 3', insert (Q70L) Sequence file: RDB15031_A7Ega.seq
>D04556G7_A7Eg_2_mStr-C_F_G02_07_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNCN NGGNNNTGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG    61 CGGCATGGNC GAGCTGTACA AGTCCGGACT CAGATCCATG GGCACCCGCG ACGACGAGTA   121 CGACTACCTC TTTAAAGTTG TCCTTATTGG AGATTCTGGT GTTGGAAAGA GTAACCTCCT   181 GTCTCGATTT ACTCGAAATG AGTTTAATCT GGAAAGCAAG AGTACCATTG GAGTAGAGTT   241 TGCAACAAGA AGCATCCAGG TTGATGGGAA AACAATAAAG GCACAGATAT GGGACACAGC   301 AGGGCTCGAG CGGTACAGGG CTATAACGTC TGCATACTAT CGTGGAGCAG TAGGTGCCTT   361 ATTGGTTTAT GACATTGCTA AGCATCTCAC ATATGAAAAT GTAGAGCGAT GGCTGAAAGA   421 ACTGAGAGAT CATGCTGATA GTAACATTGT TATCATGCTT GTGGGCAATA AGAGTGATTT   481 ACGTCATCTC AGGGCAGTTC CTACAGATGA AGCAAGAGCT TTTGCAGAGA AGAATGGTTT   541 GTCATTCATT GAGACATCTG CTCTAGATTC TACAAATGTT GAAGCTGCTT TTCAGACAAT   601 TCTAACAGAG ATATACCGCA TTGTTTCTCA GAAGCAAATG TCAGACAGAC GTGAAAATGA   661 CATGTCTCCA AGCAACAATG TGGTTCCTAT TCATGTTCCG CCCACCACTG AGAACAAGCC   721 AAAGGTGCAG TGCTGTCAGA ACATCTAAAA TCACTAGTGA ATTCGCGGCC GCCTGCAGGT   781 CGACGGTACC GCGGGCCCGG GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC   841 CACATTTGTA GAGGTTTTAC TTGCTTTNAA AAACCTCCCA CACNNNCCCC NGAACNTGAA   901 ACATAAAATG AATGCAATTG TTGTTGTTAN CTTGTTTATT GNAGCTTATN NTGGNTNCNA   961 ATAAAGCNNN NGCNNCNNNA NNTTNNCNAA TNAAGCNNTT TTTTTNNNNN NNNTNNNNNN 1021 NNNN //
Primer: SV40polyA-R2 Region: SV40 pA, insert 3' (Q70L) Sequence file: RDB15031_A7Egb.seq
>D04556G7_A7Eg_2_SV40polyA_R2_H02_23_ABI24.ab1     1 NNNNNNNNNN NNNGNNNNNN NNNNNTTAAC AACNACNATT GCATTCNTTT TATGTTTCNG    61 GTTCNGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG   121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACCTG   181 CAGGCGGCCG CGAATTCACT AGTGATTTTA GATGTTCTGA CAGCACTGCA CCTTTGGCTT   241 GTTCTCAGTG GTGGGCGGAA CATGAATAGG AACCACATTG TTGCTTGGAG ACATGTCATT   301 TTCACGTCTG TCTGACATTT GCTTCTGAGA AACAATGCGG TATATCTCTG TTAGAATTGT   361 CTGAAAAGCA GCTTCAACAT TTGTAGAATC TAGAGCAGAT GTCTCAATGA ATGACAAACC   421 ATTCTTCTCT GCAAAAGCTC TTGCTTCATC TGTAGGAACT GCCCTGAGAT GACGTAAATC   481 ACTCTTATTG CCCACAAGCA TGATAACAAT GTTACTATCA GCATGATCTC TCAGTTCTTT   541 CAGCCATCGC TCTACATTTT CATATGTGAG ATGCTTAGCA ATGTCATAAA CCAATAAGGC   601 ACCTACTGCT CCACGATAGT ATGCAGACGT TATAGCCCTG TACCGCTCGA GCCCTGCTGT   661 GTCCCATATC TGTGCCTTTA TTGTTTTCCC ATCAACCTGG ATGCTTCTTG TTGCAAACTC   721 TACTCCAATG GTACTCTTGC TTTCCAGATT AAACTCATTT CGAGTAAATC GAGACAGGAN   781 GTTACTCTTT CCAACACCAG AATCTCNNAT AANGACAACT TTAAAGAGGT AGTCGTACTC   841 GTCGTCGCGG GNGCCCATGG NTCTGANTNC GGANTTGNAC AGCTCGTCCA TGNCGCCNGN   901 NGANTGGCGG NCCTCNGCGC GTNCGTACNG TTCCNCGANG GTGNANNCCN CGNNGNGGNA   961 NGNGANGTNC NANTTGATGN NNANNANGNN NNNNCNNGNN NNCTGCNNNG GNNNCNNNNN 1021 NNNNN //
Primer: CMV_Forward Region: CMV pro, mStr 5' Sequence file: RDB15031_A7Egc.seq
>D04556G7_A7Eg_2_CMV_Forward_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TNCGGNGGGN NGTCTATATA    61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA   121 GCAAGGGCGA GGAGAATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA   181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT   241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT   301 GGGACATCCT AACCCCCAAC TTCACCTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG   361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA   421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT   481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA   541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA   601 AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGANGTCA   661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACATC GTCGGCATCA   721 AGTTGGACAT CACCTCCCAC AACGAGGANT ACACCATCGT GGAACTGTAC GAACGCGCCG   781 ANGGCCGCCA CTCCACCGGC GGCNTGGNCG ANCTGTACAA GTCCGGANTC ANATNCNTGG   841 GNACCCGCGA CGACNANTAC GACTACNNCT TTNANGTTGN NCTTATTGGA NANTCTGGNN 901 NNGGAAANAN TAANCNNCNN NCNCNANTTA NNNNNANNNN NNTNNNNNNN NNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, NeoR_KanR Sequence file: RDB15031_A7Egd.seq
>D04556G7_A7Eg_2_SV40pro_ori_F_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCCT CNGCCTCTNN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC   601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG   661 ANGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC   721 GCTTTTCTGG ANTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAN   781 CGTTGGCTAN NNGNGATATT GCTGAAGANC TTGGCGGCGA ATGGGCTGAN NGCTNCCTCN   841 NGCTTTACNG TATCGCCNCT CCCNANNCNC ANCGCATCGC CNTCNATCGN NNNCNNNNNN 901 ANNNNNNNNN ANNNGNNNNN NNGGNNNTNN NAANNGANNN NNNNANNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl