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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pmStr-C1-mouse Rab11A-Q70L

Expression vector of mouse constitutively active (CA) mutant Rab11A (Q70L), fused with N-terminal mStr, CMV promoter.

Catalog number RDB15031
Resource name pmStr-C1-mouse Rab11A-Q70L
Clone info. Expression vector of mouse constitutively active (CA) mutant Rab11A (Q70L), fused with N-terminal mStr, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pmStr-C1 (plasmid)
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Rab11a cDNA
Reference of nucleotide sequence AB232605 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Rab11a (NCBI Gene 53869) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
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RDB15031 pmStr-C1-mouse Rab11A-Q70L DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Rab family protein (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15031_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mStr-C_F
Region: mStr 3', insert (Q70L)
Sequence file: RDB15031_A7Ega.seq check
>D04556G7_A7Eg_2_mStr-C_F_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNCN NGGNNNTGTA CGAACGCGCC GAGGGCCGCC ACTCCACCGG
   61 CGGCATGGNC GAGCTGTACA AGTCCGGACT CAGATCCATG GGCACCCGCG ACGACGAGTA
  121 CGACTACCTC TTTAAAGTTG TCCTTATTGG AGATTCTGGT GTTGGAAAGA GTAACCTCCT
  181 GTCTCGATTT ACTCGAAATG AGTTTAATCT GGAAAGCAAG AGTACCATTG GAGTAGAGTT
  241 TGCAACAAGA AGCATCCAGG TTGATGGGAA AACAATAAAG GCACAGATAT GGGACACAGC
  301 AGGGCTCGAG CGGTACAGGG CTATAACGTC TGCATACTAT CGTGGAGCAG TAGGTGCCTT
  361 ATTGGTTTAT GACATTGCTA AGCATCTCAC ATATGAAAAT GTAGAGCGAT GGCTGAAAGA
  421 ACTGAGAGAT CATGCTGATA GTAACATTGT TATCATGCTT GTGGGCAATA AGAGTGATTT
  481 ACGTCATCTC AGGGCAGTTC CTACAGATGA AGCAAGAGCT TTTGCAGAGA AGAATGGTTT
  541 GTCATTCATT GAGACATCTG CTCTAGATTC TACAAATGTT GAAGCTGCTT TTCAGACAAT
  601 TCTAACAGAG ATATACCGCA TTGTTTCTCA GAAGCAAATG TCAGACAGAC GTGAAAATGA
  661 CATGTCTCCA AGCAACAATG TGGTTCCTAT TCATGTTCCG CCCACCACTG AGAACAAGCC
  721 AAAGGTGCAG TGCTGTCAGA ACATCTAAAA TCACTAGTGA ATTCGCGGCC GCCTGCAGGT
  781 CGACGGTACC GCGGGCCCGG GATCCACCGG ATCTAGATAA CTGATCATAA TCAGCCATAC
  841 CACATTTGTA GAGGTTTTAC TTGCTTTNAA AAACCTCCCA CACNNNCCCC NGAACNTGAA
  901 ACATAAAATG AATGCAATTG TTGTTGTTAN CTTGTTTATT GNAGCTTATN NTGGNTNCNA
  961 ATAAAGCNNN NGCNNCNNNA NNTTNNCNAA TNAAGCNNTT TTTTTNNNNN NNNTNNNNNN
 1021 NNNNGNNTNN NNNNNANNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNN
//
Primer: SV40polyA-R2
Region: SV40 pA, insert 3' (Q70L)
Sequence file: RDB15031_A7Egb.seq check
>D04556G7_A7Eg_2_SV40polyA_R2_H02_23_ABI24.ab1
    1 NNNNNNNNNN NNNGNNNNNN NNNNNTTAAC AACNACNATT GCATTCNTTT TATGTTTCNG
   61 GTTCNGGGGG AGGTGTGGGA GGTTTTTTAA AGCAAGTAAA ACCTCTACAA ATGTGGTATG
  121 GCTGATTATG ATCAGTTATC TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACCTG
  181 CAGGCGGCCG CGAATTCACT AGTGATTTTA GATGTTCTGA CAGCACTGCA CCTTTGGCTT
  241 GTTCTCAGTG GTGGGCGGAA CATGAATAGG AACCACATTG TTGCTTGGAG ACATGTCATT
  301 TTCACGTCTG TCTGACATTT GCTTCTGAGA AACAATGCGG TATATCTCTG TTAGAATTGT
  361 CTGAAAAGCA GCTTCAACAT TTGTAGAATC TAGAGCAGAT GTCTCAATGA ATGACAAACC
  421 ATTCTTCTCT GCAAAAGCTC TTGCTTCATC TGTAGGAACT GCCCTGAGAT GACGTAAATC
  481 ACTCTTATTG CCCACAAGCA TGATAACAAT GTTACTATCA GCATGATCTC TCAGTTCTTT
  541 CAGCCATCGC TCTACATTTT CATATGTGAG ATGCTTAGCA ATGTCATAAA CCAATAAGGC
  601 ACCTACTGCT CCACGATAGT ATGCAGACGT TATAGCCCTG TACCGCTCGA GCCCTGCTGT
  661 GTCCCATATC TGTGCCTTTA TTGTTTTCCC ATCAACCTGG ATGCTTCTTG TTGCAAACTC
  721 TACTCCAATG GTACTCTTGC TTTCCAGATT AAACTCATTT CGAGTAAATC GAGACAGGAN
  781 GTTACTCTTT CCAACACCAG AATCTCNNAT AANGACAACT TTAAAGAGGT AGTCGTACTC
  841 GTCGTCGCGG GNGCCCATGG NTCTGANTNC GGANTTGNAC AGCTCGTCCA TGNCGCCNGN
  901 NGANTGGCGG NCCTCNGCGC GTNCGTACNG TTCCNCGANG GTGNANNCCN CGNNGNGGNA
  961 NGNGANGTNC NANTTGATGN NNANNANGNN NNNNCNNGNN NNCTGCNNNG GNNNCNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1081 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1141 NNNNNNNNNN NNNNNNNNNN NN
//
Primer: CMV_Forward
Region: CMV pro, mStr 5'
Sequence file: RDB15031_A7Egc.seq check
>D04556G7_A7Eg_2_CMV_Forward_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNTNNN NGCNNTGGGC GGTANGCGTG TNCGGNGGGN NGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC ACCATGGTGA
  121 GCAAGGGCGA GGAGAATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCGCA
  181 TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT
  241 ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT
  301 GGGACATCCT AACCCCCAAC TTCACCTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG
  361 ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA
  421 ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT
  481 TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA
  541 AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA
  601 AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGANGTCA
  661 AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACATC GTCGGCATCA
  721 AGTTGGACAT CACCTCCCAC AACGAGGANT ACACCATCGT GGAACTGTAC GAACGCGCCG
  781 ANGGCCGCCA CTCCACCGGC GGCNTGGNCG ANCTGTACAA GTCCGGANTC ANATNCNTGG
  841 GNACCCGCGA CGACNANTAC GACTACNNCT TTNANGTTGN NCTTATTGGA NANTCTGGNN
  901 NNGGAAANAN TAANCNNCNN NCNCNANTTA NNNNNANNNN NNTNNNNNNN NNNNNNNNNN
  961 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
 1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1081 NNNNNNNNNN
//
Primer: SV40pro_ori_F
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB15031_A7Egd.seq check
>D04556G7_A7Eg_2_SV40pro_ori_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCCT CNGCCTCTNN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT
   61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA
  121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT
  181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC
  241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG
  301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG
  361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC
  421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC
  481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG
  541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC
  601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG
  661 ANGNTCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC
  721 GCTTTTCTGG ANTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAN
  781 CGTTGGCTAN NNGNGATATT GCTGAAGANC TTGGCGGCGA ATGGGCTGAN NGCTNCCTCN
  841 NGCTTTACNG TATCGCCNCT CCCNANNCNC ANCGCATCGC CNTCNATCGN NNNCNNNNNN
  901 ANNNNNNNNN ANNNGNNNNN NNGGNNNTNN NAANNGANNN NNNNANNNNN NNNNNANNCN
  961 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
1021 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198.
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2020.01.09

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