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pEGFP-C1-human USP6NL

Expression vector of human ubiquitin specific peptidase 6 N-terminal like (USP6NL), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB15025
Resource name pEGFP-C1-human USP6NL
Alternative name RN-tre
KIAA0019
Clone info. Expression vector of human ubiquitin specific peptidase 6 N-terminal like (USP6NL), fused with N-terminal EGFP, CMV promoter.
Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human USP6NL cDNA
Reference of nucleotide sequence AB449916 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank human USP6NL (NCBI Gene 9712) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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Schedule A (GFP-A) [Link]
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RDB15025 pEGFP-C1-human USP6NL DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15025_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB15025_A7Ega.seq check
>D04556G1_A7Eg_1_EGFP-C2_F03_18_ABI24.ab1
    1 TAACCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGAATTCAG ACCAGGATGT
  121 AGCACTCAAA CTTGCCCAGG AGCGAGCTGA AATAGTTGCT AAATATGACA GAGGACGAGA
  181 AGGTGCAGAG ATTGAACCTT GGGAAGATGC TGATTACCTT GTTTACAAAG TCACAGATAG
  241 ATTTGGCTTT TTACATGAGG AGGAGCTCCC AGATCATAAT GTGGCTGTGG AACGGCAAAA
  301 GCACCTGGAA ATTGAAAGAA CTACCAAATG GCTGAAAATG CTGAAAGGAT GGGAAAAATA
  361 CAAGAACACT GAAAAGTTTC ATAGGCGAAT TTACAAAGGA ATACCACTCC AGCTCAGAGG
  421 TGAAGTCTGG GCCCTCCTTC TTGAGATCCC TAAAATGAAA GAAGAAACAA GGGACCTGTA
  481 TAGTAAATTA AAACACAGAG CACGGGGCTG TTCACCTGAC ATCAGACAAA TAGACCTGGA
  541 TGTCAACCGC ACATTTCGGG ACCACATTAT GTTTAGAGAC AGATATGGTG TTAAGCAACA
  601 ATCCTTATTC CATGTGCTTG CTGCCTATTC TATTTATAAC ACGGAAGTCG GGTATTGTCA
  661 GGGGATGAGC CAGATCACAG CTTTACTCCT CATGTATATG AACGAGGAAG ATGCCTTCTG
  721 GGCCCTGGTC AAACTCTTCT CAGGCCCTAA ACATGCCATG CATGGCTTTT TTGTCCAAGG
  781 TTTTCCTAAA CTCTTGAGGT TTCAAGAACA TCATGAAAAA ATACTGAACA AATTTCTGTC
  841 CAAGCTTAAG CAACACTTGG ATTCTCAAGA AATCTACACA AGTTTTTACA CAATGAAATG
  901 GTTTTTTCAG TGTTTCCTTG ATCGTACTCC CTTTACACTA AACCTCAGAA TATGGGATAT
  961 CTACATCTTT GAAGGAGAAC GAGTTTCTTT ACTGCTATGT CTTTACACCA TCTTTAAAAT
 1021 TACACAAAAA AAACATCTAA ATGAAATTGT CATGCAGACT TGTAGAATTT TTTCCATGAG
 1081 ACCTTACAAA GATTTTGTGA GATGATTTGT GATAGAGCCA CCTTCAGAAT TTCTTATTGA
1141 CCG
//
Primer: SV40polyA-R
Sequence file: RDB15025_A7Egb.seq check
>D04556G1_A7Eg_1_SV40polyA-R_G03_21_ABI24.ab1
    1 CAATGCAGTT TTAGCAGTAA ACCTCTACAA ATGTGGTATG GCTGATTATG ATCAGTTATC
   61 TAGATCCGGT GGATCCCGGG CCCGCGGTAC CGTCGACTAC ATTCCATAAA GAGGACAGAA
  121 ATGGATTGTA TAAATGGCCA CCCTGGGACC TTCGTGGGGA GAAGCCATGT GAGTCGAGGC
  181 AGAAGAAAGA AGTAACCACA CTGCTAATGC AGTTAGTGTG GCTGCCCCAC ATGAGTACTG
  241 TGAGCACTGA GGGATCAGTA AGCTGCAACT TCTCCTGCTC TGAGATTCGC AAGAGTCAGA
  301 CCAGAACAAA GGGCACAAAC GCACTAAGAC CAATCTCTTA GTATGTGGAT GTGATAAGCA
  361 CTGCTGCACC TTTGGCCGTA AACTTATTTT AAGTGCCCCG ATTTTTAAGC ACCATTAAGT
  421 GGTTAGCCTT TCTCTCCAAT GTCTTTAATT TTCAGATTGA AAGAAAATGG CAACAGCTGA
  481 AGGAGAATGA TGAGCATTCT GTGAGACTGC AATTAATAAA GTCAGGACAA ACTCTTCAGT
  541 CAGTTTAGGA CCCAGGTTAC AAATATATGA GAGGGAAAAA GAAAAACTGG ACTAAAAGAC
  601 AAGGAAAACA AGAATTACAT ATTATTCCAT GGATCTGGAT TAAAAGTAGT TTTTTAAAAA
  661 ACGTTTCTTT AGCTCTGTGT TCAAATGTGG ATGAACAGAT GCCTATATCT ATTAAAAGAC
  721 ACATACACTA CATATAGCCT TAAAAATATC TATTCTACAG TTTTTTCAGT ACTTTGTAAC
  781 ATTAGTCAAA ATAGAATGAG CCAACAGAGT AAAAATGTTC AGGTTTTGGA GAAAAACATA
  841 ACCGGAACTG AGTAATAAAT TACCACTGTG CGTGCATCCC TAAATGCTAT TGCGATGTTT
  901 CCGGGTTAAA TTCTAACAGG TCAGTGATGA TGCAATTGAA ACGCTCATCT TAAGCAGCAT
  961 CTACGTGGGG CTGAAGACAT TTCCCTGTAT TCTTATTACT AACAGACAGG AGAGATGTGC
 1021 GAGTTTGTTT ACAATAGTAT AAATACTGCT TTGGGCAATT ATGGAACATA GCATGTAGTT
 1081 TCACGTGTTT CTCTTCTCGG TCCTTTAGCC AAGTACACGT CCAAATCTCA CAGTCATCAC
1141 TGTCTCATGC ATGTGGAAC
//
Primer: RDB15025#1
Sequence file: RDB15025_A7Egc.seq check
>D04556G1_A7Eg_1_RDB15025#1_A12_01_ABI08.ab1
    1 TCCTTTTTTG GCCAAAATTG AAACATAGCA ATGTAGGTTT CACGTGGTTT CTCTCTCGTC
   61 TTTAGCAAGT ACACGTCAAA TCTCACAGCA ACACTGACTC TTGGATGGAA AGCCCGTCCC
  121 GATTCCTGTA GTGGTAGGCT GGAGGCGGGG GCCCTGAATA TGGATATCCA GATGGACTGG
  181 CATCTTCTGC GGCCGGTGAA GCTTTATATC TCACGGGACT ATCTACAGAA ACTGCAGGGA
  241 GGCCATGGTC CTGAAAGGGT GCGAGTTGAA AGGCTGAGTA TTTTGGTAAA TTGCCACGGC
  301 TAGCATCTCG GGTCCATGAT TGTCCCTGCG TCTCAGGTCT GTATGTATAA CTAACTTCTG
  361 ACCATGTTCT GTTATCTGGC AAGTAATCCA CTGGTGGAAT GATCAATTTT CCATTCTTTG
  421 GTGACCCTGA ATTGCCCGAA TATCCCCCAG CACCAGTGTC AACAGGGAGG ACTTCTATTC
  481 GACTAGACGG CAGTACAAGG GGGCTTGGGC GGCTGTAAGA TTTCTCCGGA GAAGCACTGA
  541 CGGAAAGAGT AGAACCATGA GGTCTCCTGG AAGGATTCAG TTGAGTCCCA GGGCTAAACT
  601 GTGGAGAAGC AAAGCTGCTG TTGGCAGTAG GGAAGTGTTT GGGAGAGTTT CCGTGGTAAA
  661 CGGGGGGATT GCTGTAGGAG GGGGGATGAG CTAGCCCTCG GGCTTCCCCA TCTAGCTGGG
  721 ACGGATATCG TGCATGACTT GGAGGCTGTA CTTTAAAAGT AAAACTTGTT GGATACTTTT
  781 GATGGACTAG AACTTGGCTC AGCGTGCTTT CTCGGGCTGG GAGGGTAAAG GGCATGCCGG
  841 GGGGCTCTGG GAGTAAGCCC TTTCCAGCGC CTCCTCCACG GAAGCGCCGC TGTCCAGCTC
  901 CGGGCCCTGG CACGTTGTCG TACTGCGATG CAGTGGAGCC CCGCTTCCCG TCCTCAGCAT
  961 CCAGGGCCTT CATCTTTGGG CCGCAACGTT TGACACCCGC ACCTCGGCAG GACCTGGGAC
 1021 GGGTAACTGC CGAGCGCGGG GTGCGCTGCC TCGACCCTTT GCCTTTCCAT GGAGTATTTG
 1081 GCAGTTCTCT CTGTAGCTGA GACGTCTGAC GGTTTATTCC ATTTGGCACA ACTCCTCTGA
1141 TATTTGAAGT GGCGGTTGCC TATTTTTGGG TTGTGGCAGA GCCTAT
//
Primer: SV40pro_F_V2
Sequence file: RDB15025_A7Egd.seq check
>D04556G1_A7Eg_1_SV40pro_F_V2_H03_24_ABI24.ab1
    1 GACGAGCTTT TTTGGAGCCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCGGGACGCC GGCTGGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGA
 1021 GTTCTTTCGC CCACCCCTCG GGGAGGCTAA CTGAAACACG GAGAGACATA CTGCATGACC
1081 CGCGCTATGA CGGCAATTAA AGACCGTATT AAAACGCCAC GCTGTTTGGG ATCCGATTG
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

2019.12.12

GNP_filter3_RDBDEP_html_191130.pl