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pEGFP-C1-human TBC1D25 (#RDB15013)

Expression vector of human TBC1 domain family member 25 (TBC1D25), fused with N-terminal EGFP, CMV promoter.

Alternative name OATL1
Clone info. Expression vector of human TBC1 domain family member 25 (TBC1D25), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D25 cDNA
Reference of insert sequence AB449912 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15013 pEGFP-C1-human TBC1D25 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D25 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15013).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15013_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 ()
Sequence file: RDB15013_A7Ega.seq check
>D04556E7_1_EGFP-C2_A02_02_ABI24.ab1
    1 GGACCAACGA GCAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGCAACA GCCTCCGGGG
  121 CCTCGGACTT GTCTGGCTCC GGAGCGCCCC CGCCCGGTGT GGGAGCTCAG GCGGCGGCGG
  181 CCGCTGAGGA GGAGGAGCGA GAGGTGGTGC GGGTCCGAGT CAAGAAATGT GAGAGCTTCT
  241 TGCCGCCAGA GTTCCGCTCT TTTGCTGTAG ATCCCCAGAT CACCTCGCTC GACGTGTTGC
  301 AGCACATCCT CATCCGAGCC TTTGATTTGA GTGGGAAGAA GAACTTTGGC ATCAGCTACC
  361 TGGGCCGGGA CCGGCTAGGA CAGGAAGTTT ACCTCTCACT TCTATCTGAC TGGGACCTCA
  421 GCACAGCCTT CGCCACTGCC TCCAAACCTT ACCTGCAATT GCGTGTAGAT ATTCGGCCCT
  481 CGGAGGACAG CCCATTGCTA GAAGACTGGG ACATAATCAG CCCCAAAGAT GTCATTGGCT
  541 CCGACGTGTT GCTGGCTGAG AAACGGTCAT CACTGACGAC TGCCGCCCTG CCCTTTACAC
  601 AGTCCATCCT CACTCAGGTG GGCCGTACCT TGTCTAAGGT CCAACAAGTG CTGAGCTGGT
  661 CGTATGGGGA AGATGTCAAG CCCTTCAAGC CACCCCTGAG CGATGCTGAG TTTCACACGT
  721 ACCTGAACCA CGAGGGCCAG CTCTCCCGAC CCGAGGAGCT GCGCCTGCGG ATCTATCATG
  781 GCGGTGTGGA GCCCTCGCTG CGAAAGGTGG TGTGGCGGTA CCTGCTGAAC GTGTATCCAG
  841 ATGGACTGAC AGGCCGAGAG CGGATGGACT ACATGAAACG CAAGAGCCGC GAGTATGAGC
  901 AGCTCAAGAG CGAGTGGGCC CAGCGAGCGA GCCCTGAGGA CCTGGAAATT CATCCGCAGC
  961 ACGGTCCTCA AGGATGTACT GCGCACTGAC CCGGGCCCAC CCCTACTATG CGGGGCCCTG
 1021 AGGATGGGCC CACATCTACG GGCGCTGCAC GAACTGCCTC AACACTATGC CGATACCCAC
1081 CACAGGTGTC TTACCTGCAA GGCCATGGAA AGGAACGTTG GCGTA
//
Primer: pAxEF_R2 ()
Sequence file: RDB15013_A7Egb.seq check
>D04556E7_1_pAxEF_R2_B02_05_ABI24.ab1
    1 GGGGTAAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
   61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
  121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
  181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
  241 GCGAATTCAC TAGTGATTCA AGATGCGGCT GTGGCCTCAG CCCCCTCCTC TGAGTCCCAC
  301 ACCTCTGACT GCAGGTAATC AGCAAAGAGA GCCCTAGCCC GGCGCAGGAC GCGCCCCAGG
  361 TGGTGTTTTC GCACAAGGCG GTCAAAGTGC ATGGCCAGCT CATTATAATC CAGCCCATTG
  421 CGCATGATGT GGTCGCGGTG CTCCAGCAGG ATGGCCAGGC AGAGGAACAG CATGAATGGG
  481 TTCCCCCGGC CAAACTCCTG GGGTGGGGGC AGGCCCATTG GTGGTACAGG TGGCAGGGAC
  541 TTTCCAGGGT CTTTCGGGGA GCCTACCTCT TGCATCAAGG GGGATCCTAC AGCCATATCA
  601 CCAGTGGGAG AGGCCTCCTG GGTGGATGGT GGAGATGAGG AAGATGGGGA ATCAGGGTGG
  661 GAAAAGGAGG AGAGCAGTGG GTCTGGGGAG TTCAATAAAG GCTCAGAGAG GGACTTGGAG
  721 CTGATAAGGG CAGCTGGGTG GGGCAGCTGG ACCAGAGGGT CCCTCCTGGA GCCCATGTTA
  781 TCCCTGAGTT GCTGGAGGCC ATCCAAGCTG GCCTGTCTCA GGAGACGCCC CCCACCACCA
  841 GGCCCCTGAC TGGCTGTGGC CAGGTGGTCA ACAGCATCTT CAAAGGTACT ACCTCCTCCA
  901 CCAGCAGGCC TCAGCATGTG CCTCTGTCGC ACGGGCCACC CCCTGTGGCC ACCAAAACCA
  961 GTGTCTGCCA CTTGGCTGGG GGGGTCCAAC CAGCTCTACC TCATGTTTCA GGAGGATCAT
 1021 GGGGCAGCGA ACTCCAAAGT GACCCTCAAG GCATGGCGAG GCATCGGTCG GAGGCGACTC
1081 CAGCTGAGTC AGCAGCAGCC AGCCGGTAAC AAGGAA
//
Primer: CMV_Forward ()
Sequence file: RDB15013_A7Egc.seq check
>D04556E7_1_CMV Forward_C02_08_ABI24.ab1
    1 CGTACAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACAGGTGGG AGGTCTATAT
   61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
  121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
  181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
  241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
  301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
  361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
  421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
  481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
  541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
  601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
  661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
  721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
  781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
  841 AGATCTATGG CAACAGCCTC CGGGGCCTCG GACTTGTCTG GCTCCGGAGC GCCCCCGCCC
  901 GGTGTGGGAG CTCACGCGGC GGCGGCCGCT GAGGAGGAGG AGCGAGAGGT GGTGCGGGGT
  961 CCGAGTCAAG AATGTGAGAG CTTTCTTGCC CGCCCAGAGT TTCCCGCTCT TTTTGCTGTA
 1021 GATCCCCAGA TCACTCGCTC GGACGTTGGT GCCAGCACAT GTCCATCCGA GTCCATGAAT
1081 TGAGTTGGGA AGAAGAACTT TTGGCCATCC AGAC
//
Primer: f1ori_R3 ()
Sequence file: RDB15013_A7Egd.seq check
>D04556E7_1_f1ori_R3_A04_01_ABI08.ab1
    1 GGCCCGGGGG CATTTTTGGG GGGATGTGCG CGGACCCCTA TTTGTTTATT TTTCTAAATA
   61 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
  121 AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG
  181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
  241 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC
  301 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
  361 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
  421 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
  481 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT
  541 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA
  601 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
  661 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG
  721 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT
  781 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT
  841 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG
  901 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
  961 ACTCGGATGG AAGCCGGTCT TGTCGATCAA GGATGATCTG GGACGAAGAG CATCAAGGGG
 1021 CTCGCGCAGC CGAACTGTTC GCCAGGCTCA AAGGCGAGCA TGCCGACGGC GAGGATCTCG
 1081 GTCGTGACCC AATGACGAAT GCTGCTGCGA TATCATGCTG AAATGGCGCT TTTCTGGAAA
1141 TTCAATTCCG AACTGTTT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]