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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D25

Expression vector of human TBC1 domain family member 25 (TBC1D25), fused with N-terminal EGFP, CMV promoter.

Catalog number RDB15013
Resource name pEGFP-C1-human TBC1D25
Alternative name OATL1
Clone info. Expression vector of human TBC1 domain family member 25 (TBC1D25), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D25 cDNA
Reference of insert sequence AB449912 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
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External Database
human TBC1D25

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
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          RDB15013 pEGFP-C1-human TBC1D25 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15013_A7Egp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 ()
          Sequence file: RDB15013_A7Ega.seq check
          >D04556E7_1_EGFP-C2_A02_02_ABI24.ab1
              1 GGACCAACGA GCAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
             61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC TATGGCAACA GCCTCCGGGG
            121 CCTCGGACTT GTCTGGCTCC GGAGCGCCCC CGCCCGGTGT GGGAGCTCAG GCGGCGGCGG
            181 CCGCTGAGGA GGAGGAGCGA GAGGTGGTGC GGGTCCGAGT CAAGAAATGT GAGAGCTTCT
            241 TGCCGCCAGA GTTCCGCTCT TTTGCTGTAG ATCCCCAGAT CACCTCGCTC GACGTGTTGC
            301 AGCACATCCT CATCCGAGCC TTTGATTTGA GTGGGAAGAA GAACTTTGGC ATCAGCTACC
            361 TGGGCCGGGA CCGGCTAGGA CAGGAAGTTT ACCTCTCACT TCTATCTGAC TGGGACCTCA
            421 GCACAGCCTT CGCCACTGCC TCCAAACCTT ACCTGCAATT GCGTGTAGAT ATTCGGCCCT
            481 CGGAGGACAG CCCATTGCTA GAAGACTGGG ACATAATCAG CCCCAAAGAT GTCATTGGCT
            541 CCGACGTGTT GCTGGCTGAG AAACGGTCAT CACTGACGAC TGCCGCCCTG CCCTTTACAC
            601 AGTCCATCCT CACTCAGGTG GGCCGTACCT TGTCTAAGGT CCAACAAGTG CTGAGCTGGT
            661 CGTATGGGGA AGATGTCAAG CCCTTCAAGC CACCCCTGAG CGATGCTGAG TTTCACACGT
            721 ACCTGAACCA CGAGGGCCAG CTCTCCCGAC CCGAGGAGCT GCGCCTGCGG ATCTATCATG
            781 GCGGTGTGGA GCCCTCGCTG CGAAAGGTGG TGTGGCGGTA CCTGCTGAAC GTGTATCCAG
            841 ATGGACTGAC AGGCCGAGAG CGGATGGACT ACATGAAACG CAAGAGCCGC GAGTATGAGC
            901 AGCTCAAGAG CGAGTGGGCC CAGCGAGCGA GCCCTGAGGA CCTGGAAATT CATCCGCAGC
            961 ACGGTCCTCA AGGATGTACT GCGCACTGAC CCGGGCCCAC CCCTACTATG CGGGGCCCTG
           1021 AGGATGGGCC CACATCTACG GGCGCTGCAC GAACTGCCTC AACACTATGC CGATACCCAC
          1081 CACAGGTGTC TTACCTGCAA GGCCATGGAA AGGAACGTTG GCGTA
          //
          Primer: pAxEF_R2 ()
          Sequence file: RDB15013_A7Egb.seq check
          >D04556E7_1_pAxEF_R2_B02_05_ABI24.ab1
              1 GGGGTAAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
             61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
            121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
            181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
            241 GCGAATTCAC TAGTGATTCA AGATGCGGCT GTGGCCTCAG CCCCCTCCTC TGAGTCCCAC
            301 ACCTCTGACT GCAGGTAATC AGCAAAGAGA GCCCTAGCCC GGCGCAGGAC GCGCCCCAGG
            361 TGGTGTTTTC GCACAAGGCG GTCAAAGTGC ATGGCCAGCT CATTATAATC CAGCCCATTG
            421 CGCATGATGT GGTCGCGGTG CTCCAGCAGG ATGGCCAGGC AGAGGAACAG CATGAATGGG
            481 TTCCCCCGGC CAAACTCCTG GGGTGGGGGC AGGCCCATTG GTGGTACAGG TGGCAGGGAC
            541 TTTCCAGGGT CTTTCGGGGA GCCTACCTCT TGCATCAAGG GGGATCCTAC AGCCATATCA
            601 CCAGTGGGAG AGGCCTCCTG GGTGGATGGT GGAGATGAGG AAGATGGGGA ATCAGGGTGG
            661 GAAAAGGAGG AGAGCAGTGG GTCTGGGGAG TTCAATAAAG GCTCAGAGAG GGACTTGGAG
            721 CTGATAAGGG CAGCTGGGTG GGGCAGCTGG ACCAGAGGGT CCCTCCTGGA GCCCATGTTA
            781 TCCCTGAGTT GCTGGAGGCC ATCCAAGCTG GCCTGTCTCA GGAGACGCCC CCCACCACCA
            841 GGCCCCTGAC TGGCTGTGGC CAGGTGGTCA ACAGCATCTT CAAAGGTACT ACCTCCTCCA
            901 CCAGCAGGCC TCAGCATGTG CCTCTGTCGC ACGGGCCACC CCCTGTGGCC ACCAAAACCA
            961 GTGTCTGCCA CTTGGCTGGG GGGGTCCAAC CAGCTCTACC TCATGTTTCA GGAGGATCAT
           1021 GGGGCAGCGA ACTCCAAAGT GACCCTCAAG GCATGGCGAG GCATCGGTCG GAGGCGACTC
          1081 CAGCTGAGTC AGCAGCAGCC AGCCGGTAAC AAGGAA
          //
          Primer: CMV_Forward ()
          Sequence file: RDB15013_A7Egc.seq check
          >D04556E7_1_CMV Forward_C02_08_ABI24.ab1
              1 CGTACAACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACAGGTGGG AGGTCTATAT
             61 AAGCAGAGCT GGTTTAGTGA ACCGTCAGAT CCGCTAGCGC TACCGGTCGC CACCATGGTG
            121 AGCAAGGGCG AGGAGCTGTT CACCGGGGTG GTGCCCATCC TGGTCGAGCT GGACGGCGAC
            181 GTAAACGGCC ACAAGTTCAG CGTGTCCGGC GAGGGCGAGG GCGATGCCAC CTACGGCAAG
            241 CTGACCCTGA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC CACCCTCGTG
            301 ACCACCCTGA CCTACGGCGT GCAGTGCTTC AGCCGCTACC CCGACCACAT GAAGCAGCAC
            361 GACTTCTTCA AGTCCGCCAT GCCCGAAGGC TACGTCCAGG AGCGCACCAT CTTCTTCAAG
            421 GACGACGGCA ACTACAAGAC CCGCGCCGAG GTGAAGTTCG AGGGCGACAC CCTGGTGAAC
            481 CGCATCGAGC TGAAGGGCAT CGACTTCAAG GAGGACGGCA ACATCCTGGG GCACAAGCTG
            541 GAGTACAACT ACAACAGCCA CAACGTCTAT ATCATGGCCG ACAAGCAGAA GAACGGCATC
            601 AAGGTGAACT TCAAGATCCG CCACAACATC GAGGACGGCA GCGTGCAGCT CGCCGACCAC
            661 TACCAGCAGA ACACCCCCAT CGGCGACGGC CCCGTGCTGC TGCCCGACAA CCACTACCTG
            721 AGCACCCAGT CCGCCCTGAG CAAAGACCCC AACGAGAAGC GCGATCACAT GGTCCTGCTG
            781 GAGTTCGTGA CCGCCGCCGG GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC
            841 AGATCTATGG CAACAGCCTC CGGGGCCTCG GACTTGTCTG GCTCCGGAGC GCCCCCGCCC
            901 GGTGTGGGAG CTCACGCGGC GGCGGCCGCT GAGGAGGAGG AGCGAGAGGT GGTGCGGGGT
            961 CCGAGTCAAG AATGTGAGAG CTTTCTTGCC CGCCCAGAGT TTCCCGCTCT TTTTGCTGTA
           1021 GATCCCCAGA TCACTCGCTC GGACGTTGGT GCCAGCACAT GTCCATCCGA GTCCATGAAT
          1081 TGAGTTGGGA AGAAGAACTT TTGGCCATCC AGAC
          //
          Primer: f1ori_R3 ()
          Sequence file: RDB15013_A7Egd.seq check
          >D04556E7_1_f1ori_R3_A04_01_ABI08.ab1
              1 GGCCCGGGGG CATTTTTGGG GGGATGTGCG CGGACCCCTA TTTGTTTATT TTTCTAAATA
             61 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA
            121 AAAAGGAAGA GTCCTGAGGC GGAAAGAACC AGCTGTGGAA TGTGTGTCAG TTAGGGTGTG
            181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG
            241 CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC
            301 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC
            361 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG
            421 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG
            481 GCTTTTGCAA AGATCGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT
            541 GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA
            601 CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG
            661 GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAAGA CGAGGCAGCG
            721 CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT
            781 GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT
            841 CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG
            901 CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT
            961 ACTCGGATGG AAGCCGGTCT TGTCGATCAA GGATGATCTG GGACGAAGAG CATCAAGGGG
           1021 CTCGCGCAGC CGAACTGTTC GCCAGGCTCA AAGGCGAGCA TGCCGACGGC GAGGATCTCG
           1081 GTCGTGACCC AATGACGAAT GCTGCTGCGA TATCATGCTG AAATGGCGCT TTTCTGGAAA
          1141 TTCAATTCCG AACTGTTT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
          reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl