Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D22B

Expression vector of human TBC1 domain family member 22B (TBC1D22B), fused with N-terminal EGFP, CMV promoter.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)

Ordering

Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします （Schedule AとSchedule Bを入手[link])。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15010_A7Egp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB15010_A7Ega.seq
>D04556E4_1_EGFP-C2_E01_13_ABI24.ab1     1 GTACCAACCG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA    61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCCGCT GAGAACAGCA   121 AGCAGTTTTG GAAGAGGAGC GCTAAGCTGC CGGGGAGCAT TCAGCCTGTA TATGGAGCAC   181 AGCATCCTCC TCTTGACCCA CGGCTCACCA AAAATTTCAT TAAAGAACGA TCAAAAGTCA   241 ACACAGTTCC TCTGAAGAAT AAGAAGGCCT CCAGTTTTCA TGAGTTTGCA CGGAATACCA   301 GTGATGCTTG GGACATTGGC GATGATGAGG AAGAGGACTT TTCCTCACCT TCTTTCCAAA   361 CTCTGAACTC AAAAGTTGCT TTGGCAACTG CAGCCCAAGT TCTAGAAAAC CACAGCAAGC   421 TGAGAGTAAA ACCAGAACGG TCCCAGTCAA CGACATCGGA CGTCCCTGCC AACTACAAGG   481 TCATAAAGTC CAGCAGTGAT GCCCAGCTGT CCAGAAACTC TAGTGATACA TGCCTGAGGA   541 ACCCACTCCA CAAACAGCAA TCACTCCCTC TCCGGCCCAT CATCCCCCTC GTTGCCCGGA   601 TCTCGGATCA GAACGCTTCT GGGGCCCCCC CAATGACTGT CCGGGAGAAA ACCCGCCTAG   661 AAAAATTCCG TCAGCTTCTC TCCAGCCAGA ACACTGACTT AGATGAACTG AGGAAGTGTA   721 GCTGGCCAGG GGTTCCCAGG GAGGTTCGAC CTATAACCTG GAGACTCCTG TCGGGCTATC   781 TCCCAGCAAA CACTGAGAGG AGGAAGTTGA CCCTGCAGCG GAAGCGGGAG GAATATTTTG   841 GCTTCATTGA ACAGTATTAT GACTCTCGAA ACGAGGAACA TCACCAGGAT ACCTACAGAC   901 AGATTCACAT TGACATTCCA AAGGACGAAT CCTCTCATTC CCGTTGTTCC AGCAACCCAC   961 TTGTACAGGG AGATCTTTGA AAGAATTTCT ATTTATTTGG GGCCATCCGC CAACCCTGCC  1021 AGTGGGGTAT GTCAAGGGAA TAAATGACCC TGATCACTTC AATCGTTGTC GTCCTCTCTC 1081 AGATATGTGA GAGAATGTGG AGAACCTTTG AACGGTGCAC A //
Primer: pAxEF_R2 Sequence file: RDB15010_A7Egb.seq
>D04556E4_1_pAxEF_R2_F01_16_ABI24.ab1     1 GAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT    61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG   121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA   181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACC TGCAGGCGGC   241 CGCGGGAATT CGATTCTATC GGCGGTAGTG ATTTGGGGCA TCGGCAAACA TGTACTTGAG   301 TCTGTATGCC TCGGCGAGAA GCAGCCCAAT TTCTTCGTTG CCCCAGTGTA TTGTAGGTAG   361 GTTCTGTAGC AGCATGAGGA GACCCTGAAA ATCCTCCTCA TCCAAGATCT CTTTCCTCCA   421 CTTGATCAAG AAGGCTGCAC ACACGTAGAG ATGAAAGTGG GAGAACCCTT CTGGTTCAGA   481 CTGATATGTG TCCCACAGGC GGATGGTGCA GCGAAGAGGA AGCTCCCGCA TAAGCAGGTT   541 GTTCATCCAG CGGAAGGCAA ACTGCAGGTA TTCTACCTCG TACCTCCTGA AGTGATTATG   601 TACCTGCTCA TCAATCCGGC TGACAAGCTC TTCCAGTGCC TTCACCTTCT TCTGGATTCC   661 TGGTTGTGCA AAGGTGTAGT TATCCTGGAT TCCATCCAGC AGCTTGCTCA TGCACCAAAA   721 GCTGTCAGCC TCAATGCTTC GCAGCATGTC TTGAGACAAG TTGGTCACGT CAAAGTTCTC   781 CACATCCTCT TCCACATATT CTGAGAGGAA GACGACAAAG AATGGAGTGA CCAGGTCATT   841 AATTCCCTGG ACATACCCAC TGGCAGGGTG GCGGATGGCC CAAATAAATA GAATTCTTTC   901 AAGATCTCCT GTACAAGTGG TTGCTGGAAC AACGGAATGA GAGGATTCGT CCCTTGGAAT   961 GTCAATGTGA ATCTGTCTGT AGGTATCCCT ATGATGTTCT CGTTTCGAGA GTCATATACC  1021 TGGTCATGAG CTAAATATTC CTTCCGCTCG CTGCAGGATC ACTTCTCTCT CAGTGATGCT 1081 GGAGAATAGC CGGACAGGGA AGTC //
Primer: CMV_Forward Sequence file: RDB15010_A7Egc.seq
>D04556E4_1_CMV Forward_G01_19_ABI24.ab1     1 TCTTCCCCTT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG   121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG   181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA   241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG   301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC   361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA   421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA   481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC   541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA   601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC   661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC   721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC   781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC   841 TCAGATCCAT GGCCGCTGAG AACAGCAAGC AGTTTTGGAA GAGGAGCGCT AAGCTGCCGG   901 GGAGCATTCA GCCTGTATAT GGAGCACAGC ATCCTCCTCT TGACCCACGG CTCACCAAAA   961 ATTTCATTAA AGAACGATCA AAAGTCAACA CAGTTCCTCT GAGATAAGAA GGCTCAAGTT  1021 TTTCATGAGT TTGCACGCAT ACAGTTGATG CTTTGGGACA TGACGATGAT GAGAGAGAAC 1081 TTTTTCCCTC AACCGTTTCT //
Primer: f1ori_R3 Sequence file: RDB15010_A7Egd.seq
>D04556E4_1_f1ori_R3_H01_22_ABI24.ab1     1 GGGCAGTCAT TTTCGGGGAA TGTGCGCGGA CCCCTATTTG TTTATTTTTC TAAATACATT    61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA   121 GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA   181 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC   241 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA   301 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG   361 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC   421 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT   481 TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT   541 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC   601 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC   661 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC   721 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG   781 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC   841 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG   901 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC   961 GGATGGAAGC CGGGTCTTGT CGATCAGATG ATCTGGACGA AGAGCATCAT GGGCTCGCGC  1021 CAGCCCGAAA CTGGTTCGGC AGCTCAAGCG AGCATGCCCG AACGGCGAGA TCTCGTCGTG 1081 ACCCATGCGA TGCCTTGCGT GCCGATATCA ATGGTGAAAA TGGCCCGCCC AT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.07.13

GNP_filter3_RDBDEP_html_220713.pl