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pEGFP-C1-human TBC1D22B (#RDB15010)

Expression vector of human TBC1 domain family member 22B (TBC1D22B), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ20337
Clone info. Expression vector of human TBC1 domain family member 22B (TBC1D22B), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D22B cDNA
Reference of insert sequence AB449909 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)
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手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)。

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RDB15010 pEGFP-C1-human TBC1D22B DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D22B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15010).

Reference section:

Ishibashi, K., Kanno, E., Itoh, T., Fukuda, M., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15010_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 ()
Sequence file: RDB15010_A7Ega.seq check
>D04556E4_1_EGFP-C2_E01_13_ABI24.ab1
    1 GTACCAACCG AGAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
   61 CTCTCGGCAT GGACGAGCTG TACAAGTCCG GACTCAGATC CATGGCCGCT GAGAACAGCA
  121 AGCAGTTTTG GAAGAGGAGC GCTAAGCTGC CGGGGAGCAT TCAGCCTGTA TATGGAGCAC
  181 AGCATCCTCC TCTTGACCCA CGGCTCACCA AAAATTTCAT TAAAGAACGA TCAAAAGTCA
  241 ACACAGTTCC TCTGAAGAAT AAGAAGGCCT CCAGTTTTCA TGAGTTTGCA CGGAATACCA
  301 GTGATGCTTG GGACATTGGC GATGATGAGG AAGAGGACTT TTCCTCACCT TCTTTCCAAA
  361 CTCTGAACTC AAAAGTTGCT TTGGCAACTG CAGCCCAAGT TCTAGAAAAC CACAGCAAGC
  421 TGAGAGTAAA ACCAGAACGG TCCCAGTCAA CGACATCGGA CGTCCCTGCC AACTACAAGG
  481 TCATAAAGTC CAGCAGTGAT GCCCAGCTGT CCAGAAACTC TAGTGATACA TGCCTGAGGA
  541 ACCCACTCCA CAAACAGCAA TCACTCCCTC TCCGGCCCAT CATCCCCCTC GTTGCCCGGA
  601 TCTCGGATCA GAACGCTTCT GGGGCCCCCC CAATGACTGT CCGGGAGAAA ACCCGCCTAG
  661 AAAAATTCCG TCAGCTTCTC TCCAGCCAGA ACACTGACTT AGATGAACTG AGGAAGTGTA
  721 GCTGGCCAGG GGTTCCCAGG GAGGTTCGAC CTATAACCTG GAGACTCCTG TCGGGCTATC
  781 TCCCAGCAAA CACTGAGAGG AGGAAGTTGA CCCTGCAGCG GAAGCGGGAG GAATATTTTG
  841 GCTTCATTGA ACAGTATTAT GACTCTCGAA ACGAGGAACA TCACCAGGAT ACCTACAGAC
  901 AGATTCACAT TGACATTCCA AAGGACGAAT CCTCTCATTC CCGTTGTTCC AGCAACCCAC
  961 TTGTACAGGG AGATCTTTGA AAGAATTTCT ATTTATTTGG GGCCATCCGC CAACCCTGCC
 1021 AGTGGGGTAT GTCAAGGGAA TAAATGACCC TGATCACTTC AATCGTTGTC GTCCTCTCTC
1081 AGATATGTGA GAGAATGTGG AGAACCTTTG AACGGTGCAC A
//
Primer: pAxEF_R2 ()
Sequence file: RDB15010_A7Egb.seq check
>D04556E4_1_pAxEF_R2_F01_16_ABI24.ab1
    1 GAGGGTCAAA ATGCTTTATT TGTGAATTTG TGATGCTATT GCTTTATTTG TAACCATTAT
   61 AAGCTGCAAT AAACAAGTTA ACAACAACAA TTGCATTCAT TTTATGTTTC AGGTTCAGGG
  121 GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA TGGCTGATTA
  181 TGATCAGTTA TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACC TGCAGGCGGC
  241 CGCGGGAATT CGATTCTATC GGCGGTAGTG ATTTGGGGCA TCGGCAAACA TGTACTTGAG
  301 TCTGTATGCC TCGGCGAGAA GCAGCCCAAT TTCTTCGTTG CCCCAGTGTA TTGTAGGTAG
  361 GTTCTGTAGC AGCATGAGGA GACCCTGAAA ATCCTCCTCA TCCAAGATCT CTTTCCTCCA
  421 CTTGATCAAG AAGGCTGCAC ACACGTAGAG ATGAAAGTGG GAGAACCCTT CTGGTTCAGA
  481 CTGATATGTG TCCCACAGGC GGATGGTGCA GCGAAGAGGA AGCTCCCGCA TAAGCAGGTT
  541 GTTCATCCAG CGGAAGGCAA ACTGCAGGTA TTCTACCTCG TACCTCCTGA AGTGATTATG
  601 TACCTGCTCA TCAATCCGGC TGACAAGCTC TTCCAGTGCC TTCACCTTCT TCTGGATTCC
  661 TGGTTGTGCA AAGGTGTAGT TATCCTGGAT TCCATCCAGC AGCTTGCTCA TGCACCAAAA
  721 GCTGTCAGCC TCAATGCTTC GCAGCATGTC TTGAGACAAG TTGGTCACGT CAAAGTTCTC
  781 CACATCCTCT TCCACATATT CTGAGAGGAA GACGACAAAG AATGGAGTGA CCAGGTCATT
  841 AATTCCCTGG ACATACCCAC TGGCAGGGTG GCGGATGGCC CAAATAAATA GAATTCTTTC
  901 AAGATCTCCT GTACAAGTGG TTGCTGGAAC AACGGAATGA GAGGATTCGT CCCTTGGAAT
  961 GTCAATGTGA ATCTGTCTGT AGGTATCCCT ATGATGTTCT CGTTTCGAGA GTCATATACC
 1021 TGGTCATGAG CTAAATATTC CTTCCGCTCG CTGCAGGATC ACTTCTCTCT CAGTGATGCT
1081 GGAGAATAGC CGGACAGGGA AGTC
//
Primer: CMV_Forward ()
Sequence file: RDB15010_A7Egc.seq check
>D04556E4_1_CMV Forward_G01_19_ABI24.ab1
    1 TCTTCCCCTT CCGCCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGTC GCCACCATGG
  121 TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG CTGGACGGCG
  181 ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA
  241 AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG
  301 TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC
  361 ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA
  421 AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA
  481 ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC
  541 TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA
  601 TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC
  661 ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC
  721 TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC
  781 TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTCCGGAC
  841 TCAGATCCAT GGCCGCTGAG AACAGCAAGC AGTTTTGGAA GAGGAGCGCT AAGCTGCCGG
  901 GGAGCATTCA GCCTGTATAT GGAGCACAGC ATCCTCCTCT TGACCCACGG CTCACCAAAA
  961 ATTTCATTAA AGAACGATCA AAAGTCAACA CAGTTCCTCT GAGATAAGAA GGCTCAAGTT
 1021 TTTCATGAGT TTGCACGCAT ACAGTTGATG CTTTGGGACA TGACGATGAT GAGAGAGAAC
1081 TTTTTCCCTC AACCGTTTCT
//
Primer: f1ori_R3 ()
Sequence file: RDB15010_A7Egd.seq check
>D04556E4_1_f1ori_R3_H01_22_ABI24.ab1
    1 GGGCAGTCAT TTTCGGGGAA TGTGCGCGGA CCCCTATTTG TTTATTTTTC TAAATACATT
   61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
  121 GGAAGAGTCC TGAGGCGGAA AGAACCAGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA
  181 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
  241 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA
  301 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG
  361 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC
  421 CGCCTCGGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
  481 TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  541 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  601 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  661 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC
  721 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  781 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  841 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  901 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  961 GGATGGAAGC CGGGTCTTGT CGATCAGATG ATCTGGACGA AGAGCATCAT GGGCTCGCGC
 1021 CAGCCCGAAA CTGGTTCGGC AGCTCAAGCG AGCATGCCCG AACGGCGAGA TCTCGTCGTG
1081 ACCCATGCGA TGCCTTGCGT GCCGATATCA ATGGTGAAAA TGGCCCGCCC AT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishibashi, K., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]