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pEGFP-C1-human TBC1D19 (#RDB15006)

Expression vector of human TBC1 domain family member 19 (TBC1D19), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ11082
Clone info. Expression vector of human TBC1 domain family member 19 (TBC1D19), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D19 cDNA
Reference of insert sequence AB449905 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB15006 pEGFP-C1-human TBC1D19 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D19 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15006).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15006_B0Hvp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB15006_B0Hva.seq check
>D04556D9_B0Hv_2_EGFP-C2_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNNTG GNNCCTGCTG GNGTTCGTGN NCGCCGCCGG
   61 GATCACTCTC GGCATGGACG AGCTGTACAA GTCCGGACTC AGATCCATGT TGCAGGAGGA
  121 GTCGGACCTC TCTCTCATTA TTGCCCAGAT AGTCCAAAAG CTCAAGGGCT CCAATTTGTA
  181 CTCTCAGCTG GAACGGCAGG CCTGGGCCAG TCTTCAGAGA CCTGAGATTA AACTTGAATC
  241 ACTGAAAGAA GATATTAAGG AATTCTTTAA AATATCAGGT TGGGAGAAGA AACTTCAGAA
  301 TGCTGTTTAT AGTGAACTGA GTGTGTTTCC TTTACCTAGT CATCCTGCTG CACCTCCTGA
  361 ACATCTTAAA GAACCTTTGG TATACATGAG GAAAGCACAG GGAAGTTGGG AAAAAAGAAT
  421 TTTGAAGAGT TTAAATAGTA TGTGCACTGA ACTGAGTATC CCACTGGCAC GAAAGAGGCC
  481 AGTTGGAGAA CAGAAAGAAC TTCTTAATAA ATGGAATGAA ATGGGAACTG ATGAACCAGA
  541 TTTAAGCCTT TTCAGACCTG TTTATGCACC TAAGGATTTT CTTGAGGTAT TAATTAATCT
  601 TCGCAACCCA AATTATGAAA ACGGTGATTC TCTTAGTTTC AGGACTCATT TGGGTTTAAT
  661 TCAAGTTCCA CTGAAAGTAA AAGACATCCC TGAATTGAAA GAATGCTTTG TGGAACTTGG
  721 CTTAAATATA GGACAACTGG GTATAGATGA TTCTACACAA GTGCCTCCTG AACTTTTTGA
  781 AAATGAGCAT GTACGTATTG GGCAAAAAGT TCTAGCAGAA CAAGANAGTG CTGCTGCTCA
  841 ACAGTACATC AGACAAGGAA GTCCCACGGN ACTGANAGCT GANNTGNGGG CTCTNNTTTT
  901 GAATATTNNN NGCNNANNNN ANGNTGTCTT GTATTATGAN CAGCTNNNGA NNNATGTGNN
961 NCNNNATGNN NTTTTGGTGG NNANNCTNNT CNATAANNNN NNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB15006_B0Hvb.seq check
>D04556D9_B0Hv_2_SV40polyA_R2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNA NNNANNNNTT AACNACNACA ATTGNATTCN TTTTATGTTT
   61 CAGGTTCAGG GGGAGGTGTG GGAGGTTTTT TAAAGCAAGT AAAACCTCTA CAAATGTGGT
  121 ATGGCTGATT ATGATCAGTT ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC
  181 TGCAGAATTC ACTAGTGATT TCAGGTGACA GTAGCAAACA GAAAAATCTG AAGAAGAGGC
  241 ATAACTTTTA AAGTAGAAAG GTCAGCAAGA ACTGCTTCAG CTGCAGCCAG TGATGTCACC
  301 TCCATCAGGT TCACTGCTCG GAAAGCAAAC ACGGCAGCTG CCAGCACAGC AAGAATTTCC
  361 AGAGAGTTGT ATCCTAGGAT TCTATCCCAT AAAAGCAAGA GCTGATCTGT AGCTAAGTAT
  421 CCAGAGAAAG CTCGAACCAT CCACTTAAAT GATATGCGAA GTGGTTGAGC CCCAATTTCT
  481 CGTAGATGAT AAAAGAGTTG GGGAAGATAA GTTTGAAGAA GAGTTTCAAA CAGCAGACAG
  541 AGTGACACAA TACCAGAAGG ATGAGAAGAG ATGGAATGGA GTCTGAAGAA AAAACGCACA
  601 TACATCTCAC GGAATATCTG ATACAATTTG GAAGGTTCAT GGTATAGAAA ACAAAGTGGT
  661 GCAACATACA TTGAAAATCC ATGAAAAGGG ATAACACCAT TAGGTGGGTA AAAGACAGCA
  721 TATTCTTCCA GTCCTAGTTT TCCTCTTATG TATGATTTTG GTGGCGAGGC ACTGTTGAAT
  781 GCAAAGTGAC TCAACACANA TGTATCNCGG GAAAAACAAA GTAATACCTG ATATAAATAA
  841 TCTTCAAATA CNAAATAATA ATCATCATTG CTNGCTGTCA ACTTCACATC TTTTATAGAT
  901 TAGACTGTCC NNCAAANNGN CATGTTGTAT CACATNNGNC TTAAGCTGCT CATNATACAA
961 GACATNCNCN NGNTNNNNTG NNAATNNTCA AAATNANNNN CNNNNNNNNN N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB15006_B0Hvc.seq check
>D04556D9_B0Hv_2_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNNNNNNNGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
  121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
  181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
  241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
  301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
  361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
  421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
  481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
  541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
  601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
  661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
  721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
  781 GGAGTTCGTG ANCGCCGCCG GGATCACTCT CGNCATGGAC GANCTGTACA AGTCCGGACT
  841 CNGATCCNTG TTGCANGANG AGTCNGACCT CTCTCTCATT ATTGCCCAGA TAGTCCNAAA
  901 GCTCANNGGC TCCAATTTGN ACTCTCAGCT NGANNGGNAG GNCNTGGGCC ANNNTTCNGA
  961 NNNNTGANAN TNAACNNNNA NTCNNNNNAA GANNNNNTNN NNGNANTNNN TNNAANNNNN
1021 NNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR_KanR
Sequence file: RDB15006_B0Hvd.seq check
>D04556D9_B0Hv_2_SV40pro_ori_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GCCTCNNCCT CTGNNCTATT CCNNNAAGTA GTGAGGAGGC
   61 TTTTTTGGAG GCCTAGGCTT TTGCAAAGAT CGATCAAGAG ACAGGATGAG GATCGTTTCG
  121 CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT
  181 CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC
  241 AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT
  301 GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT
  361 GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA
  421 GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT
  481 GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG
  541 CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA
  601 AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA
  661 CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA
  721 TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA
  781 CATAGCGTTG GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT
  841 CCTCGNGCTT TACGGTATCG CCGCTCCNGA TTCGCAGCGC ATCGCNTTCT ATCGCCTTCT
  901 TGACGANTTC TNCTGANCGG GNNTCTGGGG TNCGAAATGN NCNGANNNNN CGANNCCNNA
  961 NCNTGNCNNT CACNANANTT NCNANTNNNN NNNNCCNNCN NNNNATGNAN NNTNNGGNCN
1021 TNNNNNNNNN TTNNNNGGNN NNNNGNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]