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pEGFP-C1-human TBC1D12-R559K (#RDB14999)

Expression vector of human mutant TBC1 domain family member 12 (TBC1D12), [GTPase-deficient mutant, R559K], fused with N-terminal EGFP, CMV promoter.

Alternative name R559K-GAP activity-deficient
Clone info. Expression vector of human mutant TBC1 domain family member 12 (TBC1D12), [GTPase-deficient mutant, R559K], fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D12 cDNA
Reference of insert sequence AB449898 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)
提供案内 (日本国内) [open/close]

必要書類
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Oguchi, M. E. et al. Plos One, 12(4): e0174883, 2017)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14999 pEGFP-C1-human TBC1D12-R559K Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D12-R559K was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14999).

Reference section:

Oguchi, M.E., Noguchi, K., Fukuda, M., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Oguchi, M.E., TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells. PLoS One 12 (4): e0174883 (2017). PMID 28384198. [link to RRC of NBRP]