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pEGFP-C1-human TBC1D10A (#RDB14994)

Expression vector of human TBC1 domain family member 10A (TBC1D10A), fused with N-terminal EGFP, CMV promoter.

Alternative name EPI64
Clone info. Expression vector of human TBC1 domain family member 10A (TBC1D10A), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TBC1D10A cDNA
Reference of insert sequence AB449894 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14994 pEGFP-C1-human TBC1D10A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D10A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14994).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14994_A7Egp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2
Sequence file: RDB14994_A7Ega.seq check
>D04556C6_A7Eg_2_EGFP-C2_H04_22_ABI24.ab1
    1 GCCCCACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGGCGAAGA GCAACGGAGA
  121 GAATGGGCCG CGCGCGCCCG CGGCCGGGGA AAGCCTGTCG GGAACCCGGG AGAGCCTGGC
  181 CCAGGGCCCC GACGCCGCAA CCACCGACGA ACTCAGCTCT CTCGGGTCTG ACTCGGAGGC
  241 CAACGGCTTC GCCGAGCGCC GCATCGACAA GTTCGGCTTC ATCGTGGGCT CGCAGGGCGC
  301 CGAGGGCGCG CTGGAGGAAG TACCCCTGGA GGTGCTGAGG CAGAGGGAGT CCAAGTGGCT
  361 GGACATGCTC AACAACTGGG ACAAATGGAT GGCCAAGAAG CACAAAAAGA TTCGTCTGCG
  421 GTGCCAAAAG GGCATCCCGC CTTCTCTGCG GGGCCGTGCT TGGCAGTACC TGTCAGGAGG
  481 CAAGGTGAAG TTACAGCAGA ACCCTGGAAA GTTTGACGAG CTGGACATGT CCCCTGGGGA
  541 CCCCAAGTGG CTGGACGTGA TTGAGCGTGA CCTGCACCGG CAGTTCCCAT TCCATGAGAT
  601 GTTTGTGTCC CGGGGGGGCC ACGGCCAGCA GGACCTATTC CGTGTGCTGA AGGCCTACAC
  661 GCTGTACCGG CCCGAGGAGG GCTACTGCCA GGCCCAGGCG CCCATTGCCG CTGTCTTGCT
  721 CATGCATATG CCTGCTGAGC AAGCCTTCTG GTGCCTGGTA CAGATCTGTG AGAAGTACCT
  781 GCCCGGCTAC TACAGCGAGA AACTGGAGGC GATCCAGCTG GACGGGGAGA TCCTTTTCTC
  841 GCTGTTGCAG AAAGTGTCGC CGGTGGCCCA CAAGCACCTC AGCCGTCAGA AGATCGACCC
  901 GCTCCTTTAT ATGACAGAAT GGTTCATGTG CGCCTTCTCC CGAACCTTGC CCTGCAGCTC
  961 TGTGCTGCGT GTCTGGGACA TGTTCTTCTG TGAAAGGGGT CAAGATCATC TTCCGGGTGG
 1021 GGCTGGTGCT GCTGAAGCAC CGCCGCTGGC TCCCCCTGAG AGTCAAAGGC TTGCAGGCCA
 1081 GTACGAGACA ATCGAGCGAC TGCCGAGACT CAGCCTCAGA TTCATGCCAT GAGTGCTTCA
1141 TGTCAAGGAA CG
//
Primer: SV40polyA-R
Sequence file: RDB14994_A7Egb.seq check
>D04556C6_A7Eg_2_SV40polyA-R_H05_23_ABI24.ab1
    1 AAAAATTGGG AAGTTTTTTA AGCAGTAAAA CCTCTACAAA TGTGGTATGG CTGATTATGA
   61 TCAGTTATCT AGATCCGGTG GATCCCGGGC CCGCGGTACC GTCGACCTGC AGGCGGCCGC
  121 GAATTCACTA GTGATTGGTT ACAAGTAGGT GTCCTCACTC TCTTGGGACG TCAAGCTCTC
  181 CTGGGAGCGG TGGTGGGCTG AGACCTGGGG AGCCAAATCC TGAGGGGCTG AGTCCTTGGG
  241 GGCTGAGTCC TTCGGGGGCA CATGCTGTGG GGGACATGCA TCTCCTGCAG CGGCCACCAC
  301 CATGGCTTGA TTTGGGGCTG GGGGCTTCTC CAGCTGCCCT CTCCCCTTCA TCTGTTTCCG
  361 CTGCTCCTTC TGGGCCTGCT TGGGTGGCTT GGGCTTGGCT TTGGAGCCAG GGAGGGGGGC
  421 ATCTAGGGGC AGGCGGATGG ATGGTGAAGG TTGTAGGGCA GGCCGGGGAC CAGGTTCTGC
  481 ATCCAAGATA GCCTTGGCAC CATGCAGCCT GGGCGGGGAG CGGCACTGCA GCTCACCCCG
  541 GGTCTCCTGC CAGCGCCGCA GCTGAATGAG GTGTTCGCGC TCAATCTGGC GCTCTGTCAC
  601 GGGCAACTCC ACCACCTCCT GGACCAGAAA GGCCTCCTGC ATGATCTTGG GGCTGAGGCT
  661 CCGCAGTCGC TCGATGGTCT CGTACTGGCC CTGGCAGGCT TTGACCTTCT CAGGGGAGCC
  721 CAGCGCGTGC TTCAGCAGCA CCAGCCCCAC CCGGAAGATG ATCTTGACCC CTTCACAGAA
  781 GAACATGTCC CAGACACGCA GCACAGAGCT CCAGGGCAAG GTTCGGGAGA AGGCGCACAT
  841 GAACCATTCT GTCATATAAA GGAGCGGGTC GATCTTCTGA CGGCTGAGGT GCTTGTGGGC
  901 CACCGGCGAC ACCTTCTGCA ACAGCGAGAA AAGGATCTCC CCGTCCAGCT GGATCGCCCT
  961 CCAGTTTCTC GCTGTAGTAG CCGGGCAGGT ACTTCTCACA GATCTGTACC AGGCACCAGA
 1021 GGCTTGCTCA GCAGCATATG CATGAAGCAA GACAGCGGGC ATGGGGCGCC CTGGGTCTGG
 1081 CAGGTAGTCC CTCCTTCGGA TCGGTACAGC GTGTAAGGCT TCCAGCCACA CGGAATAGGA
1141 TCCTTGGCCT GGGGACCGG
//
Primer: SV40pro_F_V2
Sequence file: RDB14994_A7Egc.seq check
>D04556C6_A7Eg_2_SV40pro_F_V2_H06_24_ABI24.ab1
    1 GTCCAGCTTT TTGGAGCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT
   61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
  121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
  181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
  241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
  301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
  361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
  421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
  481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
  541 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC
  601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
  661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
  781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
  841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC
  901 AACCTGCCAT CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA
  961 ATCGTTTTCC CGGACGCCCG GCTGGATGAT CCCTCCAGCG CGGGGATCTC ATGCTGGAGT
 1021 TCTTCGCCCA CCCTACGGGA GCTACTGAAA ACACGGGAAG AGACATACCG GCATGACCCT
1081 GCTATGACGG CATTAAAGAC TGATAAACGC CACCGGTGTT TGGGATC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]