Resource data sheet
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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-C1-human TBC1D6 (#RDB14988)

Expression vector of human TBC1 domain family member 6 (TBC1D6), fused with N-terminal EGFP, CMV promoter.

Alternative name FLJ22474/GRTP1
Clone info. Expression vector of human TBC1 domain family member 6 (TBC1D6), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human GRTP1 cDNA
Reference of insert sequence AB449887 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。

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RDB14988 pEGFP-C1-human TBC1D6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-human TBC1D6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14988).

Reference section:

Itoh, T., Fukuda, M., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14988_B0Kcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402)
Region: EGFP 3', insert 5'
Sequence file: RDB14988_B0Kca.seq check
1
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3'
Sequence file: RDB14988_B0Kcb.seq check
1
Primer: CMV_Forward (Pr0016)
Region: CMV pro, EGFP 5'
Sequence file: RDB14988_B0Kcc.seq check
1
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB14988_B0Kcd.seq check
1

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811. [link to RRC of NBRP]
reference Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123. [link to RRC of NBRP]