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pEGFP-C1-mouse TBC1D2B (#RDB14984)

Expression vector of mouse TBC1 domain family member 2B (TBC1D2B), fused with N-terminal EGFP, CMV promoter.

Alternative name mKIAA1055
Clone info. Expression vector of mouse TBC1 domain family member 2B (TBC1D2B), fused with N-terminal EGFP, CMV promoter.
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name mouse Tbc1d2b cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishibashi, K. et al. Genes Cells, 14(1): 41-52, 2009)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14984 pEGFP-C1-mouse TBC1D2B DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEGFP-C1-mouse TBC1D2B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14984).

Reference section:

Ishibashi, K., Kanno, E., Itoh, T., Fukuda, M., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14984_A7Egp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: mVenus-M_F (Pr0574)
Region: EGFP 3',insert 5'
Sequence file: RDB14984_A7Ega.seq check
>14984_14984_B2JqF_2_mVenus-M_F_G01_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNCNAGGTGA NTTCNAGNNC CGCCNCAACA TCGAGGANGG
   61 CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT
  121 GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA
  181 GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA
  241 CGAGCTGTAC AAGTCCGGAC TCAGATCTAT GCCGGGGGCC GGGGACGGCG TGGAGGAGAG
  301 CTGCAGTGGT GGTGAGGGAG CGGTACCGGG AACAGGCTCG GAGGCTGGGG CGGTGGCGGG
  361 ACGGGAGCCC TCGCGGCTGT GCGGCTACCT GCAGAAGCTG TCGGGCAAGG GCCCGTTGCG
  421 CGGCTACCGC AGCCGCTGGT TCGTGTTTGA CTCGCGCCGC TGCTACCTCT ACTACTTCAA
  481 GAGTCCCCAG GACGCTCTGC CGCTCGGCCA CCTGGACATC GCCGACGCCT GCTTCAGCTA
  541 CCAGGGCCGC GATGAGGCTG CGGAGCCCGG AGCAGATCCG CCCACACACT TCCAGGTGCA
  601 CAGCGCCGGT GCAGTCACCG TGCTCAAGGC TCCTAATCGT GAGCTCATGA CTTACTGGCT
  661 ACAGGAGCTT CAGCAGAAAA GATGGGAATA TTGCAACAGC CTTGACATGA TGAAGTGGGA
  721 CAGCANGACT TCCCCGACTC CNGGGGANTT TCCCAAGGGC CTTGTGGCCN GAGATACCAC
  781 AGATATAATT TCCCAGCACC CAAATCCTTC TGCAGAAAAN GNCAGNNNTG TGCNCGCTGN
  841 GGAAGCTGCC CCTGGAGAGC TGGNGGGAGA NNGGGCTGCT CATCAGCCNG CTCCCGGGNA
  901 TNNNAANNNC CNTCNACTTT TTACTCTCTG ANNNNNNGGG GNAATGANCT CNAAAATTNN
961 NNNNCNTCNT TNNNNNNNNG GGNNNNGNNN NNNNNNN
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB14984_A7Egb.seq check
>D04556B5_A7Eg_2_SV40polyA-R_F02_17_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNTNANACC TCTACNAATG TGGTATGGCT GATTATGATC
   61 AGTTATCTAG ATCCGGTGGA TCCCGGGCCC GCGGTACCGT CGACCTGCAG GCGGCCGCGA
  121 ATTCACTAGT GATTTCAAGT GTCTTCCTCC TCATCGCTAA CTAGCTCGCC TTTGTCAGGG
  181 CTAGTGTCCC GCTCACGCAG GAAGTCCTCT CGAATGGCCT CCAGCTCTGT CAGCTCCAGC
  241 CGGACCTTCT CCAGGTGGTA GGCTCTCCGG TTCCGGATCT GGCGCAGGGG GAAGGGGTTC
  301 AGATCCCCAA AGGAGATGCT GATCAGCTTC CTGGCATCGA GGATAGTCCG AGTGAAGTAA
  361 CGCAGATACT TGAATATGGA CATCGAATCT TGCAATTTCA GGATCTCCTC TTCCTTATAT
  421 TTAAAAAGTG CCAGGGCAAA ACGGAAAATA ACCTTTGGTC CCTCATAAAG GAAAGAGTCC
  481 CATATTTTAA AGAGGATGTC GCTGACAACG CTGTCCACAA ATACCACCAG AAACCAGTTG
  541 AAGGTGATGA GGGTGTAGTC CACTTTGTAC TGTTCAAAAT GGGTGTGCAG TCGAGGCAGC
  601 TTCTCACTCA GGAGGTCTCT GAACACCCGC TGGTCCACCT GGGATCCTAA AAGAGTCTTT
  661 GTGTAATAGT CTCGGGGCAT GAAGACTTCC ACGATGGTAA CGAGACACCA GAAGGCATCT
  721 TCTTGATCCA GGTAAAGGAG AGCCACTGCC ACCAACCTGT TCAGGCCTTG GCAGTAGCCG
  781 ATATCGGGAT TCCGCCAGGA NAANGCGAGC ANGACACTGC GCAGCTTCTG TATGCCCTCC
  841 GACGTGGGGC TGGAGTANTG NTTGTTGTTG GGCAGANTCC GGANCANGNC AAGCTCGATC
  901 TGCTNNNANG NCGGGTTCTG TTTNTNNNGA NCCTTCTGCA GCAAGGNCTG GNANTAGTCT
  961 GGCNNCNTGC TGNNNNTGNN NNTNCNNNNN NNGANGGNCN NCNCNNNNNN TNNNNNNNNN
1021 N
//
Primer: CMV_Forward (Pr0016)
Region: CMV pro,EGFP 5'
Sequence file: RDB14984_A7Egc.seq check
>14984_14984_B2JqF_2_CMV_Forward_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN ANNNNNNNNN NNNNNNGNNN NNNNNNNNNN
   61 NNNNTNNNNN NGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGTCGCC
  121 ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG
  181 GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC
  241 TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC
  301 ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG
  361 AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC
  421 TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC
  481 CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG
  541 CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG
  601 AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC
  661 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC
  721 CACTACCTGA GCACNNNNNC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG
  781 GTCCTGCTGG AGTTCGTGAC CGCCGCNGGG ATCACTCTCG GCATGGACNA NCTGTACNAG
  841 TCCNGACTCA NNNCTATGCC NGGGGGCCNG GGANGGCGTG NANGANANCT GCAGTGNTGG
  901 TGANGGANCG GTACCNGGAA CNGGCTCNNN NNNNGGGGNN GNNNGNNGGG ANNGGNNNCN
  961 NCGNNGNNGT GNNGGCNNCC NNCANANNCN NNNNGGNNAA NGGNCNNNNT NNNNNNNNNN
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB14984_A7Egd.seq check
>D04556B5_A7Eg_2_SV40pro_F_V2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNCNN NGCTTTTGCA AGNNCGATCN AGAGANAGGA TGAGGANCGT
   61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
  121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
  181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
  241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
  301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
  361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
  421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
  481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
  541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
  601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
  661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GANCGCTATC
  721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
  781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA NCGCATCGCC TTCTATCGCC
  841 TTCTTGACGA NTTCTTCTGA NCGGGANTCT GGGGGTTCGA AANGACCGAN CNAGCGACGC
  901 CNNACCTGCC NTCNCGAGAT TTCGANNCCA CCGCCNCCTT CTATGAAAGG NTGGNNTNCG
961 GNNNNNTTTN CCNGGNNGNN NGNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishibashi, K., Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells 14 (1): 41-52 (2009). PMID 19077034. [link to RRC of NBRP]