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pEF-T7-human TBC1D12

Expression vector of human TBC1 domain family member 12 (TBC1D12), [1-781 a.a], tagged with T7, EF1 promoter.

Catalog number RDB14978
Resource name pEF-T7-human TBC1D12
Alternative name KIAA0608
Clone info. Expression vector of human TBC1 domain family member 12 (TBC1D12), [1-781 a.a], tagged with T7, EF1 promoter, see Fig3B of Oguchi, M. E. et al. PLoS One, 12 (4): e0174883, 2017.
Expression was confirmed by the depositor with immunoblotting and GST-pull-down.
Vector backbone pEF-T7 (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name human TBC1D12 cDNA
Reference of nucleotide sequence AB449898 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank human TBC1D12 (NCBI Gene 23232) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. et al. J. Cell Biol., 192(5): 839-853, 2011)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14978 pEF-T7-human TBC1D12 Under QC test. Please contact us. DNA solution

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References and tips

Electronic file

Original reference

original Itoh, T., OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. J. Cell Biol. 192 (5): 839-853 (2011). PMID 21383079.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Itoh, T., OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. J. Cell Biol. 192 (5): 839-853 (2011). PMID 21383079.

2019.07.19

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