Resource data sheet
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pEF-T7-human TBC1D12 (#RDB14978)

Expression vector of human TBC1 domain family member 12 (TBC1D12), [1-781 a.a], tagged with T7, EF1 promoter.

Alternative name KIAA0608
Clone info. Expression vector of human TBC1 domain family member 12 (TBC1D12), [1-781 a.a], tagged with T7, EF1 promoter, see Fig3B of Oguchi, M. E. et al. PLoS One, 12 (4): e0174883, 2017.
Comment Expression was confirmed by the depositor with immunoblotting and GST-pull-down.
Vector backbone pEF-T7 (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name human TBC1D12 cDNA
Reference of insert sequence AB449898 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. et al. J. Cell Biol., 192(5): 839-853, 2011)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. et al. J. Cell Biol., 192(5): 839-853, 2011)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14978 pEF-T7-human TBC1D12 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-human TBC1D12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14978).

Reference section:

Itoh, T., Kanno, E., Uemura, T., Waguri, S., Fukuda, M., OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. J. Cell Biol. 192 (5): 839-853 (2011). PMID 21383079. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

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References

Original, user report and related articles

original Itoh, T., OATL1, a novel autophagosome-resident Rab33B-GAP, regulates autophagosomal maturation. J. Cell Biol. 192 (5): 839-853 (2011). PMID 21383079. [link to RRC of NBRP]