Resource data sheet
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pEF-BOS FLAG-mouse Rab29-T21N (#RDB14973)

Expression vector of mouse constitutively negative (CN) mutant Rab29 (T21N), tagged with N-terminal FLAG, EF1 promoter.

Alternative name Rab7L1
Clone info. Expression vector of mouse constitutively negative (CN) mutant Rab29 (T21N), tagged with N-terminal FLAG, EF1 promoter.
Comment Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-BOS FLAG (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name mouse Rab29 cDNA
Reference of insert sequence AB232625 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Aoki, Y. et al. Brain, 140(4): 887-897, 2017)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Aoki, Y. et al. Brain, 140(4): 887-897, 2017)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14973 pEF-BOS FLAG-mouse Rab29-T21N Under QC test. Please contact us. DNA solution

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pEF-BOS FLAG-mouse Rab29-T21N was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14973).

Reference section:

Aoki, Y., Manzano, R., Lee, Y., Dafinca, R., Aoki, M., Douglas, A.G.L., Varela, M.A., Sathyaprakash, C., Scaber, J., Barbagallo, P., Vader, P., Mäger, I., Ezzat, K., Turner, M.R., Ito, N., Gasco, S., Ohbayashi, N., El Andaloussi, S., Takeda, S., Fukuda, M., Talbot, K., Wood, M,J.A., C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia. Brain 140 (4): 887-897 (2017). PMID 28334866. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Aoki, Y., C9orf72 and RAB7L1 regulate vesicle trafficking in amyotrophic lateral sclerosis and frontotemporal dementia. Brain 140 (4): 887-897 (2017). PMID 28334866. [link to RRC of NBRP]
reference Tamura, K., Varp is a novel Rab32/38-binding protein that regulates Tyrp1 trafficking in melanocytes. Mol. Biol. Cell 20 (12): 2900-2908 (2009). PMID 19403694. [link to RRC of NBRP]