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delta N-PrP-EBFB (#RDB14969)

E.coli expression vector, C-terminal domain (residues 125 to 231) of mouse prion protein (PrP) linked to EBFP.

Clone info. E.coli expression vector, C-terminal domain (residues 125 to 231) of mouse prion protein (PrP) linked to EBFP. This clone used to utilized as the donor for the FRET analysis, see Fig. 1A, 1B and 1D of Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016.
Comment Expression was confirmed by the depositor with SDS-PAGE and FRET measurement.
Vector backbone pQBI 67 (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Prnp cDNA
Depositor|Developer Arii, Yasuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14969 delta N-PrP-EBFB Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The delta N-PrP-EBFB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14969).

Reference section:

Arii, Y., Yamaguchi, H., Yamasaki, M., Fukuoka, S., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243. [link to RRC of NBRP]