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PrP-EBFP (#RDB14968)

E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP.

Clone info. E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP. This clone used to utilized as the donor for the FRET analysis, see Fig. 1A, 1B and 1C of Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016.
Comment Expression was confirmed by the depositor with SDS-PAGE and FRET measurement.
Vector backbone pQBI 67 (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Prnp cDNA
Depositor|Developer Arii, Yasuhiro |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14968 PrP-EBFP Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The PrP-EBFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14968).

Reference section:

Arii, Y., Yamaguchi, H., Yamasaki, M., Fukuoka, S., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243. [link to RRC of NBRP]