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PrP-EBFP

E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP.

Catalog number RDB14968
Resource name PrP-EBFP
Clone info. E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP. This clone used to utilized as the donor for the FRET analysis, see Fig. 1A, 1B and 1C of Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016.
Expression was confirmed by the depositor with SDS-PAGE and FRET measurement.
Vector backbone pQBI 67 (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Prnp cDNA
Depositor Arii, Yasuhiro |
 
Other clones in our bank mouse Prnp (NCBI Gene 19122) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016).
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14968 PrP-EBFP Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

Further references such as user reports and related articles (go to bottom)

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Sequence information

This clone will be sequenced a portion and digested by restriction enzyme for examination.


References

Original, user report and related articles

original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

2019.07.19

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