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PrP-EBFP

E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP.

Catalog number RDB14968
Resource name PrP-EBFP
Clone info. E.coli expression vector, comprising full-length mouse prion protein (PrP) linked to EBFP at the C-terminus of PrP. This clone used to utilized as the donor for the FRET analysis, see Fig. 1A, 1B and 1C of Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016.
Comment Expression was confirmed by the depositor with SDS-PAGE and FRET measurement.
Vector backbone pQBI 67 (Plasmid)
Size of vector backbone 6.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Prnp cDNA
Depositor|Developer Arii, Yasuhiro |
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External Database
Mouse Prnp

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016).
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Arii, Y. et al. Biosci. Biotechnol. Biochem., 80 (4): 761-768, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB14968 PrP-EBFP Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Arii, Y., Detection of an interaction between prion protein and neuregulin I-beta1 by fluorescence resonance energy transfer analysis. Biosci. Biotechnol. Biochem. 80 (4): 761-768 (2016). PMID 26796243.

          2022.07.13

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