Resource data sheet
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pEF-T7-Syt I (Syt1) (#RDB14681)

Expression vector of mouse synaptotagmin I (Syt I) [Syt 1].


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Fukuda, M., J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887. [PubMed] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of mouse synaptotagmin I (Syt I) [Syt 1], T7-tag, EF1 promoter.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Growth conditions LB+Amp, 37oC
Gene/insert name mouse Syt1 cDNA
Reference of insert sequence D37792 (DDBJ accession number)
Depositor|Developer Fukuda, Mitsunori |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 269, 29206-29211, 1994)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Fukuda, M. et al. J. Biol. Chem. 269, 29206-29211, 1994)。
付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee
RDB14681 pEF-T7-Syt I (Syt1) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEF-T7-Syt I (Syt1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14681).

Reference section:

Fukuda, M., Aruga, J., Niinobe, M., Aimoto, S., Mikoshiba, K., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887. [PubMed] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887. [PubMed] [RRC of NBRP]