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pEF-T7-Syt I (Syt1)

Expression vector of mouse synaptotagmin I (Syt I) [Syt 1].

Catalog number RDB14681
Resource name pEF-T7-Syt I (Syt1)
Clone info. Expression vector of mouse synaptotagmin I (Syt I) [Syt 1], T7-tag, EF1 promoter.
Vector backbone pEF-T7 (plasmid)
Selectable markers Amp^r
Gene/insert name mouse Syt1 cDNA
Reference of nucleotide sequence D37792 (DDBJ accession number)
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Syt1 (NCBI Gene 20979) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fukuda, M. et al. J. Biol. Chem. 269, 29206-29211, 1994)
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14681 pEF-T7-Syt I (Syt1) DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)

References

original Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14681_A7Ddp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14681_A7Dda.seq
>D04472A8_A7Dd_2_EF1a-C_F_G04_19_ABI24.ab1
    1 ATCGTGGGAT TTTGCCCCCT TTTTTGAGTT TGGATCTTGG TTCATTCTCA AAGCCTCAGA
   61 CAGTGGTTCA AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC
  121 GCGGGAATTC GATTCCACCA TGGCTAGCAT GACTGGTGGA CAGCAAATGG GTCGCGGATC
  181 CATGGTGAGT GCCAGTCGTC CTGAGGCCCT GGCTGCCCCT GTCACCACTG TTGCGACCCT
  241 TGTCCCACAC AACGCCACTG AGCCAGCCAG TCCTGGGGAA GGGAAGGAAG ATGCCTTTTC
  301 CAAGCTGAAG CAGAAGTTTA TGAATGAACT GCATAAAATC CCATTGCCAC CGTGGGCCTT
  361 AATTGCCATA GCCATAGTTG CGGTCCTTCT AGTCGTGACC TGCTGCTTCT GTGTCTGTAA
  421 GAAATGTTTG TTCAAAAAGA AAAACAAGAA GAAGGGAAAG GAAAAGGGAG GGAAGAACGC
  481 CATTAACATG AAAGACGTGA AAGACTTAGG GAAGACCATG AAGGATCAGG CCCTTAAGGA
  541 TGACGATGCT GAAACTGGAC TGACTGATGG AGAAGAAAAG GAGGAGCCCA AGGAAGAGGA
  601 GAAACTGGGA AAGCTTCAAT ATTCACTGGA CTATGACTTC CAGAATAACC AGCTGCTGGT
  661 GGGAATCATC CAGGCTGCTG AACTGCCCGC CCTGGACATG GGAGGCACAT CTGATCCATA
  721 CGTCAAAGTC TTCCTGCTGC CCGACAAAAA GAAGAAGTTT GAGACAAAAG TCCACCGGAA
  781 AACCCTCAAT CCAGTCTTCA ATGAACAGTT TACTTTCAAG GGTGCCATAC TCGGAATTAG
  841 GTGGGCAAGA CACTGGTGAT GGCTGTGTAT GATTTTGACC GCTTCTCCAA GCACGACATC
  901 ATTGGAGAGT TCAAAGTTCC TATGAACACC GTGGATTTTT GGCCACGTCA CCCGAGGAGT
  961 TGGCCGCGAT CCTCCAGAGT TGCCTGAGAA AGGAAGAGCC AAGAGAAACT TGGGTGACAT
 1021 CTGCTTTCTC CCCTTCCGCC TACGGTCCCC TACTTGCCGG CAAGGCTGAC TGTGTCATTT
 1081 CTGGAAAGTC AAGAACCTTG AAGAAGATGG ATGTGGGTTG CCTTTATCTC TTGAATTCTC
1141 CCCT
//
Primer: hGCSF_R RDB14681_A7Ddb.seq
>D04472A8_A7Dd_2_hGCSF_R_G05_20_ABI24.ab1
    1 CTGGGGGACA TGGATCTTCT CAAGGGTCCC AGGTGGGGAC CCTCACTCTA GAGCGGCCGC
   61 ATCGAATTCT ACAAAATTGG GTTCACCCAT CAAAGATGTC GTGTGAGCAA ATCTTGGTAC
  121 TCAAAGGATT CAATAGAAAG TTCAGGTTTA AGTTGTCCTT TAAGATGTTG GTTTAACTGT
  181 ACTAGTGCCT TTCAAATTGA TATTTTTGCT GGAATGCAGA CGATACTATA TTTACCATAT
  241 AATATATTAT CAGTTCTCCT ATATCTCAGT CAATTTACAT AGTTTAAAAT AGAACATCTT
  301 ATTAAAAACA TCTAGATATA CACAGATTAG GAAACGCTTA CAACATACAA ATACACAAAC
  361 TAGAAATAAA TCTTTAGCAT ACTGGTATAT ACAACGGTGT GATACAATAC ATTTCAGTCA
  421 TGCTCTATCT ACACATCATA TTGCTTAAAA GAAGAGTAGA TTTGGAGGGC AGTGGTATGA
  481 TGAAGGGCAT AGGGGCTTTC TACACTTAAA TTTGTGCAAA TTAAAACAGG ATCTGAAGTA
  541 CCACTGGATT GTATCAGGAA TGCATGGCTG GAAACTGAAA GGACCCATAT TACTGAGGTA
  601 TTTACAGATA CTGGCTAAAG AGCACTATGT GGGCAGACGC AGAAAGGCTT CTTTTCCCCT
  661 TTACTTCTTG ACAGCCAGCA TGGCATCAAC CTCCTCCTCT ACCTGCAGAG TGTGCCACTG
  721 GGCGATGGGT CGCCGGGGGT TGGCCAGCAT GTCTGACCAG TGTCGCAGCT CTGCGCCGGT
  781 GCTGTTGTAG CCCACAAAGA CTTTGCCGAT GGCGTCGTTC TTGCCAATCT TGTCATAGTC
  841 CAAAACAGTT ACCACCACTT GCACTTTCTG GATTTGCTCG AACGGAACTT CAAAGCTGAA
  901 AGGACTCATT GTAGTACGGG TTAAAGTGTG TTCTTCTTAA TCGTTGTCTT TTTCTTCTTC
  961 AGTCTCTTGC CGTTCTGCAT CAGTGAATCT TTACATAGGA TCAGATAGGC CACCCACATC
 1021 CATTCTTCCT TCAGGTCTTG GCTTTCCAGA ATTGACACAA GTCAGCTTTG GCCGCAGTAG
1081 GCACGTAACG AGGAGAGCAG ATGTCACCCC AGGTTCCCTC CTGGGCGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Fukuda, M., Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/synaptotagmin II. J. Biol. Chem. 269 (46): 29206-29211 (1994). PMID 7961887.

2018.10.18

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