RDB14658 - T7-NLS-hCAS9-NLS-P2A-mCherry-pA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

T7-NLS-hCAS9-NLS-P2A-mCherry-pA (#RDB14658)

Cas9-poly(A) with mCherry to visualize Cas9 positive cells.

Clone info.	Cas9 and associated mCherry expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Comment	Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone	pcDNA3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Streptococcus pyogenes Cas9 genomic DNA Discosoma sp. RFP cDNA
Depositor\|Developer	DNA Bank, \|

Sequence (full)	RDB14658zzt02.seq compiled by us

Distribution information

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Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14658	T7-NLS-hCAS9-NLS-P2A-mCherry-pA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The T7-NLS-hCAS9-NLS-P2A-mCherry-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14658).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14658_A7Csp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14658_A7Csa.seq
>D04203A7_A7Cs_1_CMV-Forward_A01_01_ABI08.ab1 1 ACGTATAACT TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT 61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT 121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGTAAT ACGACTCACT 181 ATAGGGAGAG CCGCCACCAT GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC 241 CCAGCAGCCG ACAAGAAGTA CTCCATTGGG CTCGATATCG GCACAAACAG CGTCGGCTGG 301 GCCGTCATTA CGGACGAGTA CAAGGTGCCG AGCAAAAAAT TCAAAGTTCT GGGCAATACC 361 GATCGCCACA GCATAAAGAA GAACCTCATT GGCGCCCTCC TGTTCGACTC CGGGGAGACG 421 GCCGAAGCCA CGCGGCTCAA AAGAACAGCA CGGCGCAGAT ATACCCGCAG AAAGAATCGG 481 ATCTGCTACC TGCAGGAGAT CTTTAGTAAT GAGATGGCTA AGGTGGATGA CTCTTTCTTC 541 CATAGGCTGG AGGAGTCCTT TTTGGTGGAG GAGGATAAAA AGCACGAGCG CCACCCAATC 601 TTTGGCAATA TCGTGGACGA GGTGGCGTAC CATGAAAAGT ACCCAACCAT ATATCATCTG 661 AGGAAGAAGC TTGTAGACAG TACTGATAAG GCTGACTTGC GGTTGATCTA TCTCGCGCTG 721 GCGCATATGA TCAAATTTCG GGGACACTTC CTCATCGAGG GGGACCTGAA CCCAGACAAC 781 AGCGATGTCG ACAAACTCTT TATCCAACTG GTTCAGACTT ACAATCAGCT TTTCGAAGAG 841 AACCCGATCA ACGCATCCGG AGTTGACGCC AAAGCAATCC TGAGCGCTAG GCTGTCCAAA 901 TCCCGGCGGC TCGAAAACCC TCATCGCACA GCTCCCTGGG GAGAAGAAGA ACGGCCTGTT 961 TGGGTAATCT ATCGCCCTGT CACTCGGGCT GACCCCCCAC TTTAAATCTA CTTCGACCTC 1021 CCGAGATGCC AGCTTCAACT GAGCAAAGAA CACTTACGAT GATGAATCTC GACAATCTGC 1081 TGCCAGATCG GCGACAGGTA CCGCAGAACC TTGTTG //
Primer: Red-N_R Sequence file: RDB14658_A7Csb.seq
>D04203A7_A7Cs_1_Red-N_R_G02_07_ABI08.ab1 1 GGTTAGGGTG TGCACTTGAG CGCATGACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC 61 CTTGCTCACC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG GCCTGCTTCA GCAGGGAGAA 121 GTTGGTGGCC ACCTTCCTCT TCTTCTTGGG GTCAGCCCTG CTGTCTCCAC CGAGCTGAGA 181 GAGGTCGATT CTTGTTTCAT AGAGCCCCGT AATTGACTGA TGAATCAGTG TGGCGTCCAG 241 GACCTCCTTT GTAGAGGTGT ACCGCTTTCT GTCTATGGTG GTGTCGAAGT ACTTGAAGGC 301 TGCAGGCGCG CCCAAGTTGG TCAGAGTAAA CAAGTGGATA ATGTTTTCTG CCTGCTCCCT 361 GATGGGCTTA TCCCTGTGCT TATTGTAAGC AGAAAGCACC TTATCGAGGT TAGCGTCGGC 421 GAGGATCACT CTTTTGGAGA ATTCGCTTAT TTGCTCGATG ATCTCATCAA GGTAGTGTTT 481 GTGTTGTTCC ACGAACAGCT GCTTCTGCTC ATTATCTTCG GGAGACCCTT TGAGCTTTTC 541 ATAGTGGCTG GCCAGATACA AGAAATTAAC GTATTTAGAG GGCAGTGCCA GCTCGTTACC 601 TTTCTGCAGC TCGCCCGCAC TAGCGAGCAT TCGTTTCCGG CCGTTTTCAA GCTCAAAGAG 661 AGAGTACTTG GGAAGCTTAA TGATGAGGTC TTTTTTGACC TCTTTATATC CTTTCGCCTC 721 GAGAAAGTCG ATGGGGTTTT TTTCGAAGCT TGATCGCTCC ATGATTGTGA TGCCCAGCAG 781 TTCCTTGACG CTTTTGAGTT TTTTAGACTT CCCTTTCTCC ACTTTGGCCA CAACCAGTAC 841 ACTGTAAGCG ACTGTAGGAG AATCGAATCC GCCGTATTTC TTGGGGTCCC AATCTTTTTT 901 TGCGTGCGAT CAGCTTGTCG CTGTTCCTTT TTCGGAGGAT ACTTTTCCTT GGAGAAGCCT 961 CCGGGTCTGT ACTTCGGGTC TTTTTAACGA TGTTTCACCC TGCGGCATGA CAGGGACCCT 1021 TCCGGGACTG TCGCGAATCC TACCCTTTGT CCCACACGGA TTTCTCTGAT TTCCTCCGGT 1081 AATTCCGATT AGCTTGGTCG CTTCGGATTC TCCTCTCCAA TTTGGCCAA //
Primer: HSV_TK_pA_R Sequence file: RDB14658_A7Csc.seq
>D04203A7_A7Cs_1_HSV TK pA_R_F01_06_ABI08.ab1 1 ACGGGTTTTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCG TTTAAACTCA 61 TTACTAACCG GTAGGGATCG AACCCTTTCA CTTGTACAGC TCGTCCATGC CGCCGGTGGA 121 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT 181 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT 241 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT 301 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC 361 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC 421 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC 481 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA 541 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA 601 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG 661 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC 721 GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC 781 CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG 841 GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA GCCCTGCTGT CTCCACCGAG 901 CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAAAT TGACTGATGA ATCAGTGTGC 961 GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTTCTGTC TATGTGTGTC GAAGTACTTT 1021 GAAGCCTGGC AGCGCGCCAG TGGTCAGAGT TAAACCAGTG ATAATGTTTT CTGCCTGCTC 1081 TGAATGGACT AATCCTGTGC ATATTTGGTA AGGG //
Primer: f1ori_R3 Sequence file: RDB14658_A7Csd.seq
>D04203A7_A7Cs_1_f1ori_R3_D11_11_ABI24.ab1 1 GGGAAGGCAC TGACCAGCTG CGCAGATCTG CTATGGCAGG GCCTGCCGCC CCGACGTTGG 61 CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC 121 GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAT GTACAGCTAG CTTTTTTTTT 181 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 241 TTTTTTTTTT TCCCGGGGGT TTTTTTTTTT TTTTTTTTTG CCCCCAAAGG GGGGGTTTCT 301 TCCGGATTTT TCTCCTTCCG GGTTTCAATT ACCCCCCCCC TTTTAAACTC ATTAAAAACG 361 GGGGGGGATA AAACCCTTTT ATTTGTAAAC CTCCCCCATG CCCCGGGGGG GGGGGGGGCC 421 CCCGGGGGTT TTTTTTTTTT CAAAAAGGGG AACCCCCTTT GGGGGGGGGG AAGGCCAATT 481 TTATTTTAAT TTGAAGCCCG GGGAACTGCC AGGGTTTTTT GCCTTGAAGT GGGTTTTAAC 541 CCCCCCCCAA GGGGCCCCGT TTTAATTTAC CTTTGTTGAT TCCCCCTTCA GGGCCCCCCC 601 CGGGGAAATC CCCCGGAAAA GCCTCCCCCC CAGGTCCCCT CGATATAGGG CCTCGAAGGA 661 AAATTGGCCC AACTCCCTTG AAAAAACTCC CCCCCGAAGG AGAATCTGGT AAGTCACCAC 721 CCCCCCTCTC AAATTACAGC CTCTCCTTAA ACCTCGGGAG AGGAACTCTC ATAATGGGAT 781 GTGGGGGGGG TCTCCGTAGC CTGGACCTCT TAACTAGGGC ACGATTTCCG GGAAGGGGGG 841 GGGCACCTTG TACCTACTCG GGGCCTGGTG CCTCAGAGGA GCCCCTCCCC TCTCCCTGAT 901 TCAATTTTGG CCCCTGCAGA GCCTAATGAT CATGTAACTG ATACT //
Primer: SV40pro_F Sequence file: RDB14658_A7Cse
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