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T7-NLS-hCAS9-NLS-P2A-mCherry-pA

Cas9-poly(A) with mCherry to visualize Cas9 positive cells.

Catalog number RDB14658
Resource name T7-NLS-hCAS9-NLS-P2A-mCherry-pA
Clone info. Cas9 and associated mCherry expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Discosoma sp. RFP cDNA
Depositor DNA Bank, |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
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RDB14658 T7-NLS-hCAS9-NLS-P2A-mCherry-pA DNA solution

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Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14658_A7Csp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14658_A7Csa.seq
>D04203A7_A7Cs_1_CMV-Forward_A01_01_ABI08.ab1
    1 ACGTATAACT TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
   61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
  121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGTAAT ACGACTCACT
  181 ATAGGGAGAG CCGCCACCAT GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC
  241 CCAGCAGCCG ACAAGAAGTA CTCCATTGGG CTCGATATCG GCACAAACAG CGTCGGCTGG
  301 GCCGTCATTA CGGACGAGTA CAAGGTGCCG AGCAAAAAAT TCAAAGTTCT GGGCAATACC
  361 GATCGCCACA GCATAAAGAA GAACCTCATT GGCGCCCTCC TGTTCGACTC CGGGGAGACG
  421 GCCGAAGCCA CGCGGCTCAA AAGAACAGCA CGGCGCAGAT ATACCCGCAG AAAGAATCGG
  481 ATCTGCTACC TGCAGGAGAT CTTTAGTAAT GAGATGGCTA AGGTGGATGA CTCTTTCTTC
  541 CATAGGCTGG AGGAGTCCTT TTTGGTGGAG GAGGATAAAA AGCACGAGCG CCACCCAATC
  601 TTTGGCAATA TCGTGGACGA GGTGGCGTAC CATGAAAAGT ACCCAACCAT ATATCATCTG
  661 AGGAAGAAGC TTGTAGACAG TACTGATAAG GCTGACTTGC GGTTGATCTA TCTCGCGCTG
  721 GCGCATATGA TCAAATTTCG GGGACACTTC CTCATCGAGG GGGACCTGAA CCCAGACAAC
  781 AGCGATGTCG ACAAACTCTT TATCCAACTG GTTCAGACTT ACAATCAGCT TTTCGAAGAG
  841 AACCCGATCA ACGCATCCGG AGTTGACGCC AAAGCAATCC TGAGCGCTAG GCTGTCCAAA
  901 TCCCGGCGGC TCGAAAACCC TCATCGCACA GCTCCCTGGG GAGAAGAAGA ACGGCCTGTT
  961 TGGGTAATCT ATCGCCCTGT CACTCGGGCT GACCCCCCAC TTTAAATCTA CTTCGACCTC
 1021 CCGAGATGCC AGCTTCAACT GAGCAAAGAA CACTTACGAT GATGAATCTC GACAATCTGC
1081 TGCCAGATCG GCGACAGGTA CCGCAGAACC TTGTTG
//
Primer: Red-N_R RDB14658_A7Csb.seq
>D04203A7_A7Cs_1_Red-N_R_G02_07_ABI08.ab1
    1 GGTTAGGGTG TGCACTTGAG CGCATGACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC
   61 CTTGCTCACC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG GCCTGCTTCA GCAGGGAGAA
  121 GTTGGTGGCC ACCTTCCTCT TCTTCTTGGG GTCAGCCCTG CTGTCTCCAC CGAGCTGAGA
  181 GAGGTCGATT CTTGTTTCAT AGAGCCCCGT AATTGACTGA TGAATCAGTG TGGCGTCCAG
  241 GACCTCCTTT GTAGAGGTGT ACCGCTTTCT GTCTATGGTG GTGTCGAAGT ACTTGAAGGC
  301 TGCAGGCGCG CCCAAGTTGG TCAGAGTAAA CAAGTGGATA ATGTTTTCTG CCTGCTCCCT
  361 GATGGGCTTA TCCCTGTGCT TATTGTAAGC AGAAAGCACC TTATCGAGGT TAGCGTCGGC
  421 GAGGATCACT CTTTTGGAGA ATTCGCTTAT TTGCTCGATG ATCTCATCAA GGTAGTGTTT
  481 GTGTTGTTCC ACGAACAGCT GCTTCTGCTC ATTATCTTCG GGAGACCCTT TGAGCTTTTC
  541 ATAGTGGCTG GCCAGATACA AGAAATTAAC GTATTTAGAG GGCAGTGCCA GCTCGTTACC
  601 TTTCTGCAGC TCGCCCGCAC TAGCGAGCAT TCGTTTCCGG CCGTTTTCAA GCTCAAAGAG
  661 AGAGTACTTG GGAAGCTTAA TGATGAGGTC TTTTTTGACC TCTTTATATC CTTTCGCCTC
  721 GAGAAAGTCG ATGGGGTTTT TTTCGAAGCT TGATCGCTCC ATGATTGTGA TGCCCAGCAG
  781 TTCCTTGACG CTTTTGAGTT TTTTAGACTT CCCTTTCTCC ACTTTGGCCA CAACCAGTAC
  841 ACTGTAAGCG ACTGTAGGAG AATCGAATCC GCCGTATTTC TTGGGGTCCC AATCTTTTTT
  901 TGCGTGCGAT CAGCTTGTCG CTGTTCCTTT TTCGGAGGAT ACTTTTCCTT GGAGAAGCCT
  961 CCGGGTCTGT ACTTCGGGTC TTTTTAACGA TGTTTCACCC TGCGGCATGA CAGGGACCCT
 1021 TCCGGGACTG TCGCGAATCC TACCCTTTGT CCCACACGGA TTTCTCTGAT TTCCTCCGGT
1081 AATTCCGATT AGCTTGGTCG CTTCGGATTC TCCTCTCCAA TTTGGCCAA
//
Primer: HSV_TK_pA_R RDB14658_A7Csc.seq
>D04203A7_A7Cs_1_HSV TK pA_R_F01_06_ABI08.ab1
    1 ACGGGTTTTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCG TTTAAACTCA
   61 TTACTAACCG GTAGGGATCG AACCCTTTCA CTTGTACAGC TCGTCCATGC CGCCGGTGGA
  121 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
  181 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
  241 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
  301 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
  361 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC
  421 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
  481 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
  541 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA
  601 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG
  661 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
  721 GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC
  781 CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG
  841 GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA GCCCTGCTGT CTCCACCGAG
  901 CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAAAT TGACTGATGA ATCAGTGTGC
  961 GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTTCTGTC TATGTGTGTC GAAGTACTTT
 1021 GAAGCCTGGC AGCGCGCCAG TGGTCAGAGT TAAACCAGTG ATAATGTTTT CTGCCTGCTC
1081 TGAATGGACT AATCCTGTGC ATATTTGGTA AGGG
//
Primer: f1ori_R3 RDB14658_A7Csd.seq
>D04203A7_A7Cs_1_f1ori_R3_D11_11_ABI24.ab1
    1 GGGAAGGCAC TGACCAGCTG CGCAGATCTG CTATGGCAGG GCCTGCCGCC CCGACGTTGG
   61 CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC
  121 GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAT GTACAGCTAG CTTTTTTTTT
  181 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  241 TTTTTTTTTT TCCCGGGGGT TTTTTTTTTT TTTTTTTTTG CCCCCAAAGG GGGGGTTTCT
  301 TCCGGATTTT TCTCCTTCCG GGTTTCAATT ACCCCCCCCC TTTTAAACTC ATTAAAAACG
  361 GGGGGGGATA AAACCCTTTT ATTTGTAAAC CTCCCCCATG CCCCGGGGGG GGGGGGGGCC
  421 CCCGGGGGTT TTTTTTTTTT CAAAAAGGGG AACCCCCTTT GGGGGGGGGG AAGGCCAATT
  481 TTATTTTAAT TTGAAGCCCG GGGAACTGCC AGGGTTTTTT GCCTTGAAGT GGGTTTTAAC
  541 CCCCCCCCAA GGGGCCCCGT TTTAATTTAC CTTTGTTGAT TCCCCCTTCA GGGCCCCCCC
  601 CGGGGAAATC CCCCGGAAAA GCCTCCCCCC CAGGTCCCCT CGATATAGGG CCTCGAAGGA
  661 AAATTGGCCC AACTCCCTTG AAAAAACTCC CCCCCGAAGG AGAATCTGGT AAGTCACCAC
  721 CCCCCCTCTC AAATTACAGC CTCTCCTTAA ACCTCGGGAG AGGAACTCTC ATAATGGGAT
  781 GTGGGGGGGG TCTCCGTAGC CTGGACCTCT TAACTAGGGC ACGATTTCCG GGAAGGGGGG
  841 GGGCACCTTG TACCTACTCG GGGCCTGGTG CCTCAGAGGA GCCCCTCCCC TCTCCCTGAT
901 TCAATTTTGG CCCCTGCAGA GCCTAATGAT CATGTAACTG ATACT
//
Primer: SV40pro_F RDB14658_A7Cse
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.09.20

GNP_filter3_RDBDEP_html_180823.pl