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T7-NLS-hCAS9-NLS-P2A-mCherry-pA

Cas9-poly(A) with mCherry to visualize Cas9 positive cells.

Catalog number RDB14658
Resource name T7-NLS-hCAS9-NLS-P2A-mCherry-pA
Clone info. Cas9 and associated mCherry expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Comment Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Discosoma sp. RFP cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Streptococcus pyogenes Cas9 Discosoma sp. RFP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          Remarks
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
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          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          備考
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14658 T7-NLS-hCAS9-NLS-P2A-mCherry-pA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence (full) RDB14658zzt02.seq compiled by us

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14658_A7Csp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14658_A7Csa.seq check
          >D04203A7_A7Cs_1_CMV-Forward_A01_01_ABI08.ab1
              1 ACGTATAACT TCCGCCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
            121 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTCCGGA CTCTAGTAAT ACGACTCACT
            181 ATAGGGAGAG CCGCCACCAT GGCCCCAAAG AAGAAGCGGA AGGTCGGTAT CCACGGAGTC
            241 CCAGCAGCCG ACAAGAAGTA CTCCATTGGG CTCGATATCG GCACAAACAG CGTCGGCTGG
            301 GCCGTCATTA CGGACGAGTA CAAGGTGCCG AGCAAAAAAT TCAAAGTTCT GGGCAATACC
            361 GATCGCCACA GCATAAAGAA GAACCTCATT GGCGCCCTCC TGTTCGACTC CGGGGAGACG
            421 GCCGAAGCCA CGCGGCTCAA AAGAACAGCA CGGCGCAGAT ATACCCGCAG AAAGAATCGG
            481 ATCTGCTACC TGCAGGAGAT CTTTAGTAAT GAGATGGCTA AGGTGGATGA CTCTTTCTTC
            541 CATAGGCTGG AGGAGTCCTT TTTGGTGGAG GAGGATAAAA AGCACGAGCG CCACCCAATC
            601 TTTGGCAATA TCGTGGACGA GGTGGCGTAC CATGAAAAGT ACCCAACCAT ATATCATCTG
            661 AGGAAGAAGC TTGTAGACAG TACTGATAAG GCTGACTTGC GGTTGATCTA TCTCGCGCTG
            721 GCGCATATGA TCAAATTTCG GGGACACTTC CTCATCGAGG GGGACCTGAA CCCAGACAAC
            781 AGCGATGTCG ACAAACTCTT TATCCAACTG GTTCAGACTT ACAATCAGCT TTTCGAAGAG
            841 AACCCGATCA ACGCATCCGG AGTTGACGCC AAAGCAATCC TGAGCGCTAG GCTGTCCAAA
            901 TCCCGGCGGC TCGAAAACCC TCATCGCACA GCTCCCTGGG GAGAAGAAGA ACGGCCTGTT
            961 TGGGTAATCT ATCGCCCTGT CACTCGGGCT GACCCCCCAC TTTAAATCTA CTTCGACCTC
           1021 CCGAGATGCC AGCTTCAACT GAGCAAAGAA CACTTACGAT GATGAATCTC GACAATCTGC
          1081 TGCCAGATCG GCGACAGGTA CCGCAGAACC TTGTTG
          //
          Primer: Red-N_R
          Sequence file: RDB14658_A7Csb.seq check
          >D04203A7_A7Cs_1_Red-N_R_G02_07_ABI08.ab1
              1 GGTTAGGGTG TGCACTTGAG CGCATGACTC CTTGATGATG GCCATGTTAT CCTCCTCGCC
             61 CTTGCTCACC ATGGGGCCGG GGTTCTCCTC CACGTCGCCG GCCTGCTTCA GCAGGGAGAA
            121 GTTGGTGGCC ACCTTCCTCT TCTTCTTGGG GTCAGCCCTG CTGTCTCCAC CGAGCTGAGA
            181 GAGGTCGATT CTTGTTTCAT AGAGCCCCGT AATTGACTGA TGAATCAGTG TGGCGTCCAG
            241 GACCTCCTTT GTAGAGGTGT ACCGCTTTCT GTCTATGGTG GTGTCGAAGT ACTTGAAGGC
            301 TGCAGGCGCG CCCAAGTTGG TCAGAGTAAA CAAGTGGATA ATGTTTTCTG CCTGCTCCCT
            361 GATGGGCTTA TCCCTGTGCT TATTGTAAGC AGAAAGCACC TTATCGAGGT TAGCGTCGGC
            421 GAGGATCACT CTTTTGGAGA ATTCGCTTAT TTGCTCGATG ATCTCATCAA GGTAGTGTTT
            481 GTGTTGTTCC ACGAACAGCT GCTTCTGCTC ATTATCTTCG GGAGACCCTT TGAGCTTTTC
            541 ATAGTGGCTG GCCAGATACA AGAAATTAAC GTATTTAGAG GGCAGTGCCA GCTCGTTACC
            601 TTTCTGCAGC TCGCCCGCAC TAGCGAGCAT TCGTTTCCGG CCGTTTTCAA GCTCAAAGAG
            661 AGAGTACTTG GGAAGCTTAA TGATGAGGTC TTTTTTGACC TCTTTATATC CTTTCGCCTC
            721 GAGAAAGTCG ATGGGGTTTT TTTCGAAGCT TGATCGCTCC ATGATTGTGA TGCCCAGCAG
            781 TTCCTTGACG CTTTTGAGTT TTTTAGACTT CCCTTTCTCC ACTTTGGCCA CAACCAGTAC
            841 ACTGTAAGCG ACTGTAGGAG AATCGAATCC GCCGTATTTC TTGGGGTCCC AATCTTTTTT
            901 TGCGTGCGAT CAGCTTGTCG CTGTTCCTTT TTCGGAGGAT ACTTTTCCTT GGAGAAGCCT
            961 CCGGGTCTGT ACTTCGGGTC TTTTTAACGA TGTTTCACCC TGCGGCATGA CAGGGACCCT
           1021 TCCGGGACTG TCGCGAATCC TACCCTTTGT CCCACACGGA TTTCTCTGAT TTCCTCCGGT
          1081 AATTCCGATT AGCTTGGTCG CTTCGGATTC TCCTCTCCAA TTTGGCCAA
          //
          Primer: HSV_TK_pA_R
          Sequence file: RDB14658_A7Csc.seq check
          >D04203A7_A7Cs_1_HSV TK pA_R_F01_06_ABI08.ab1
              1 ACGGGTTTTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCG TTTAAACTCA
             61 TTACTAACCG GTAGGGATCG AACCCTTTCA CTTGTACAGC TCGTCCATGC CGCCGGTGGA
            121 GTGGCGGCCC TCGGCGCGTT CGTACTGTTC CACGATGGTG TAGTCCTCGT TGTGGGAGGT
            181 GATGTCCAAC TTGATGTTGA CGTTGTAGGC GCCGGGCAGC TGCACGGGCT TCTTGGCCTT
            241 GTAGGTGGTC TTGACCTCAG CGTCGTAGTG GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT
            301 GATCTCGCCC TTCAGGGCGC CGTCCTCGGG GTACATCCGC TCGGAGGAGG CCTCCCAGCC
            361 CATGGTCTTC TTCTGCATTA CGGGGCCGTC GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC
            421 CTTGTAGATG AACTCGCCGT CCTGCAGGGA GGAGTCCTGG GTCACGGTCA CCACGCCGCC
            481 GTCCTCGAAG TTCATCACGC GCTCCCACTT GAAGCCCTCG GGGAAGGACA GCTTCAAGTA
            541 GTCGGGGATG TCGGCGGGGT GCTTCACGTA GGCCTTGGAG CCGTACATGA ACTGAGGGGA
            601 CAGGATGTCC CAGGCGAAGG GCAGGGGGCC ACCCTTGGTC ACCTTCAGCT TGGCGGTCTG
            661 GGTGCCCTCG TAGGGGCGGC CCTCGCCCTC GCCCTCGATC TCGAACTCGT GGCCGTTCAC
            721 GGAGCCCTCC ATGTGCACCT TGAAGCGCAT GAACTCCTTG ATGATGGCCA TGTTATCCTC
            781 CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG TCGCCGGCCT GCTTCAGCAG
            841 GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA GCCCTGCTGT CTCCACCGAG
            901 CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAAAT TGACTGATGA ATCAGTGTGC
            961 GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTTCTGTC TATGTGTGTC GAAGTACTTT
           1021 GAAGCCTGGC AGCGCGCCAG TGGTCAGAGT TAAACCAGTG ATAATGTTTT CTGCCTGCTC
          1081 TGAATGGACT AATCCTGTGC ATATTTGGTA AGGG
          //
          Primer: f1ori_R3
          Sequence file: RDB14658_A7Csd.seq check
          >D04203A7_A7Cs_1_f1ori_R3_D11_11_ABI24.ab1
              1 GGGAAGGCAC TGACCAGCTG CGCAGATCTG CTATGGCAGG GCCTGCCGCC CCGACGTTGG
             61 CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC
            121 GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAT GTACAGCTAG CTTTTTTTTT
            181 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
            241 TTTTTTTTTT TCCCGGGGGT TTTTTTTTTT TTTTTTTTTG CCCCCAAAGG GGGGGTTTCT
            301 TCCGGATTTT TCTCCTTCCG GGTTTCAATT ACCCCCCCCC TTTTAAACTC ATTAAAAACG
            361 GGGGGGGATA AAACCCTTTT ATTTGTAAAC CTCCCCCATG CCCCGGGGGG GGGGGGGGCC
            421 CCCGGGGGTT TTTTTTTTTT CAAAAAGGGG AACCCCCTTT GGGGGGGGGG AAGGCCAATT
            481 TTATTTTAAT TTGAAGCCCG GGGAACTGCC AGGGTTTTTT GCCTTGAAGT GGGTTTTAAC
            541 CCCCCCCCAA GGGGCCCCGT TTTAATTTAC CTTTGTTGAT TCCCCCTTCA GGGCCCCCCC
            601 CGGGGAAATC CCCCGGAAAA GCCTCCCCCC CAGGTCCCCT CGATATAGGG CCTCGAAGGA
            661 AAATTGGCCC AACTCCCTTG AAAAAACTCC CCCCCGAAGG AGAATCTGGT AAGTCACCAC
            721 CCCCCCTCTC AAATTACAGC CTCTCCTTAA ACCTCGGGAG AGGAACTCTC ATAATGGGAT
            781 GTGGGGGGGG TCTCCGTAGC CTGGACCTCT TAACTAGGGC ACGATTTCCG GGAAGGGGGG
            841 GGGCACCTTG TACCTACTCG GGGCCTGGTG CCTCAGAGGA GCCCCTCCCC TCTCCCTGAT
          901 TCAATTTTGG CCCCTGCAGA GCCTAATGAT CATGTAACTG ATACT
          //
          Primer: SV40pro_F
          Sequence file: RDB14658_A7Cse check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl