RDB14657 - T7-NLS-hCAS9-NLS-P2A-EGFP-pA

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

T7-NLS-hCAS9-NLS-P2A-EGFP-pA (#RDB14657)

Cas9-poly(A) with GFP to visualize Cas9 positive cells.

Clone info.	Cas9 and associated EGFP expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Comment	Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone	pcDNA3 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Depositor\|Developer	DNA Bank, \|

Sequence (full)	RDB14657zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.

Remarks
The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

必要書類	提供依頼書 [依頼書の記入例 ] 専用MTA（CRISPR/Cas9内包遺伝子材料専用非営利学術目的）をお使いください [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14657	T7-NLS-hCAS9-NLS-P2A-EGFP-pA	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The T7-NLS-hCAS9-NLS-P2A-EGFP-pA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14657).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14657_A7Csp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward () Sequence file: RDB14657_A7Csa.seq
>D04203A6_A7Cs_1_CMV-Forward_E10_13_ABI24.ab1 1 AGTTCACTCC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA 61 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC 121 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGGACTC TAGTAATACG ACTCACTATA 181 GGGAGAGCCG CCACCATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA 241 GCAGCCGACA AGAAGTACTC CATTGGGCTC GATATCGGCA CAAACAGCGT CGGCTGGGCC 301 GTCATTACGG ACGAGTACAA GGTGCCGAGC AAAAAATTCA AAGTTCTGGG CAATACCGAT 361 CGCCACAGCA TAAAGAAGAA CCTCATTGGC GCCCTCCTGT TCGACTCCGG GGAGACGGCC 421 GAAGCCACGC GGCTCAAAAG AACAGCACGG CGCAGATATA CCCGCAGAAA GAATCGGATC 481 TGCTACCTGC AGGAGATCTT TAGTAATGAG ATGGCTAAGG TGGATGACTC TTTCTTCCAT 541 AGGCTGGAGG AGTCCTTTTT GGTGGAGGAG GATAAAAAGC ACGAGCGCCA CCCAATCTTT 601 GGCAATATCG TGGACGAGGT GGCGTACCAT GAAAAGTACC CAACCATATA TCATCTGAGG 661 AAGAAGCTTG TAGACAGTAC TGATAAGGCT GACTTGCGGT TGATCTATCT CGCGCTGGCG 721 CATATGATCA AATTTCGGGG ACACTTCCTC ATCGAGGGGG ACCTGAACCC AGACAACAGC 781 GATGTCGACA AACTCTTTAT CCAACTGGTT CAGACTTACA ATCAGCTTTT CGAAGAGAAC 841 CCGATCAACG CATCCGGAGT TGACGCCCAA GCAATCCTGA GCGCTAGGCT GTCCAAATCC 901 CGGCGGCTCG AAAACCTCAT CGCACAGCTC CCTGGGGAGA AGAAGAACGG GCCCTGTTTG 961 GGTAAATCTT TATCGCCCTG TCACTCGGGC TGACCCCCCA ACTTTAAATC TAACCTTCGA 1021 CTGCCGAGAT GGCTAAGCCT TCCAACTGAA GCCAAAGACA CTACGATGAT GGATTCTCGA 1081 CCATCCTGCC TGGGTCA //
Primer: EGFP-N () Sequence file: RDB14657_A7Csb.seq
>D04203A6_A7Cs_1_EGFP-N_F10_16_ABI24.ab1 1 GTTTACCGGG GGGACAGCTC CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG 61 TCGCCGGCCT GCTTCAGCAG GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA 121 GCCCTGCTGT CTCCACCGAG CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAATT 181 GACTGATGAA TCAGTGTGGC GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTCTGTCT 241 ATGGTGGTGT CGAAGTACTT GAAGGCTGCA GGCGCGCCCA AGTTGGTCAG AGTAAACAAG 301 TGGATAATGT TTTCTGCCTG CTCCCTGATG GGCTTATCCC TGTGCTTATT GTAAGCAGAA 361 AGCACCTTAT CGAGGTTAGC GTCGGCGAGG ATCACTCTTT TGGAGAATTC GCTTATTTGC 421 TCGATGATCT CATCAAGGTA GTGTTTGTGT TGTTCCACGA ACAGCTGCTT CTGCTCATTA 481 TCTTCGGGAG ACCCTTTGAG CTTTTCATAG TGGCTGGCCA GATACAAGAA ATTAACGTAT 541 TTAGAGGGCA GTGCCAGCTC GTTACCTTTC TGCAGCTCGC CCGCACTAGC GAGCATTCGT 601 TTCCGGCCGT TTTCAAGCTC AAAGAGAGAG TACTTGGGAA GCTTAATGAT GAGGTCTTTT 661 TTGACCTCTT TATATCCTTT CGCCTCGAGA AAGTCGATGG GGTTTTTTTC GAAGCTTGAT 721 CGCTCCATGA TTGTGATGCC CAGCAGTTCC TTGACGCTTT TGAGTTTTTT AGACTTCCCT 781 TTCTCCACTT TGGCCACAAC CAGTACACTG TAAGCGACTG TAGGAGAATC GAATCCGCCG 841 TATTTCTTGG GGTCCCAATC TTTTTTTGCG TGCGATCAGC TTGTCGCTGT TCCTTTTCGG 901 GAGGATACTT TCCTTGGAGA AGCCCTCCGG GTCTGTACTT CGGTCTTTTT AACGATGTTC 961 ACCTGCGGGC ATGGACAGGG ACCCTTCCCG GACTGTCGCG AAATCCCTAC CCTTGTCCCC 1021 AACACGAATT TCTCTTGTTT CTCCGTTTGT TTCGATAGTT GGATCGGCTT TCGATTCCTC 1081 ATGCCAGTGT AAATCTCGGT CTTGGAAAAA AAT //
Primer: EGFP-C2 () Sequence file: RDB14657_A7Csc.seq
>D04203A6_A7Cs_1_EGFP-C2_G10_19_ABI24.ab1 1 CTTCCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 61 TCTCGGCATG GACGAGCTGT ACAAGTGAAA GGGTTCGATC CCTACCGGTT AGTAATGAGT 121 TTAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA 181 TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGAAAAAA AAAAAAAAAA AAAAAAAAAA 241 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAGGCGCTA 301 GCTTTAACTT CGATCCCCCC CCGAAACCCA TTGGGGCCAA AACGCCCGCG TTTCTTCCTT 361 TTCCCCACCC CCCCCCCCAA TTCGGGTGAA GGCCCAGGGC TCGCAACCAA AGTTGGGGGG 421 GAGGGCCGGC CAAACAAATC GGCACACTGG GGTTCAAGGG GAACCCCCCC GCCCCTTGAC 481 GGCCATTACC GCGGGGGGGG GGGGTTACCC CACACTGGAC GTCACTTGGC AAGGCCCTAA 541 CGCCCCCCCC TTTGGTTTCT TCCCTCCTTT CGCCCATTTC CGGGTTTCCG TAAGTTTAAT 601 CGGGGCCCTT TAGGGTCCAA ATAGGCTTAG GCCTCACCCA AAAATTATAG GGAGGTCCTA 661 TGGCACTCCC TGAAACGTTC CCTTACTGGA CCCTTTTAAA GGACTGTTCA AGGAAACCCC 721 ACTCGGCATT TGATAAGGAT TCCATCCCTG GGAAAGCGTT CAATTCCAAT TTGGAGGGCA 781 AAGGGAATCC TTCCCAGAGA TTTGAGGGGT CTCTTACACC CGTTGAGCCC CCCC //
Primer: SV40pro_F () Sequence file: RDB14657_A7Csd.seq
>D04203A6_A7Cs_1_SV40pro_F_H01_08_ABI08.ab1 1 CTCTGCCCCT GGCCAATCAG AGGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA 61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT 121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA 181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC 241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA 301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG 361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC 421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG 481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA 541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC 601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA 661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG 721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT 781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG 841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC 901 GCCTTCTTGA CGAGTTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC 961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCACGCGCT TTCTATGGAA AGTTGGCCTT 1021 CGGATTCGGT TTCGGGACGC GGCTGATGGA TTCTCAGCGC GGGAATCTCA ATGCTGAGTC 1081 TCGCCACCTA CTTGGTTATT GCCAGCCA //
Primer: HSV_TK_pA_R () Sequence file: RDB14657_A7Cse.seq
>14657_14657_A9G1F_1_HSV_TK_pA_R_E07_05_ABI08.ab1 1 CGGGGTCTTT TCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCG TTTAAACTCA 61 TTACTAACCG GTAGGGATCG AACCCTTTCA CTTGTACAGC TCGTCCATGC CGAGAGTGAT 121 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT 181 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC 241 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG 301 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT 361 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC 421 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG 481 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG 541 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG 601 CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT 661 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC 721 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT 781 GAACAGCTCC TCGCCCTTGC TCACCATGGG GCCGGGGTTC TCCTCCACGT CGCCGGCCTG 841 CTTCAGCAGG GAGAAGTTGG TGGCCACCTT CCTCTTCTTC TTGGGGTCAG CCCTGCTGTC 901 TCCACCGAGC TGAGAGAGGT CGATTCTTGT TTCATAGAGC CCCGTTAATT GACTGATGAA 961 TCAGTGTGGC GTCCAGGACC CTCCTTTGTA GAAGGTGTAC CCGCTTTCTG TCTATGGGTG 1021 GGTGTCGAAG TACTTGGAAA GGCTGCAAGC GCGCCCAAGG TTGGTCAAGA GAGTAAACAA 1081 GTGATAATTG TTTCCTGCTT GCTCCTGAAT TGGCCTATCC TGTGCCTATG GTTAAGGCCA 1141 GGAAAAGGGC AACCCTTA //