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T7-NLS-hCAS9-NLS-P2A-EGFP-pA

Cas9-poly(A) with GFP to visualize Cas9 positive cells.

Catalog number RDB14657
Resource name T7-NLS-hCAS9-NLS-P2A-EGFP-pA
Clone info. Cas9 and associated EGFP expressing plasmid. Induction with CMV or T7 promoter. Applicable for both direct transfection into mammalian cells and T7 in-vitro translation.
Constructed from T7-NLS hCas9-pA (RDB13130, Yoshimi, K., Nat. Commun. 7: 10431, 2016. PMID 26786405).
Vector backbone pcDNA3 (Plasmid)
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA Aequorea victoria GFP cDNA
Depositor DNA Bank, |
 
Other clones in our bank Streptococcus pyogenes (KEGG orthology K09952) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
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Exclusive MTA (genome editing) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use the Exclusive MTA (genome editing). [Word]
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14657 T7-NLS-hCAS9-NLS-P2A-EGFP-pA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Genome Editing (English text)
Featured content Genome Editing (Japanese text)

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14657_A7Csp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14657_A7Csa.seq
>D04203A6_A7Cs_1_CMV-Forward_E10_13_ABI24.ab1
    1 AGTTCACTCC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC
  121 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGGACTC TAGTAATACG ACTCACTATA
  181 GGGAGAGCCG CCACCATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA
  241 GCAGCCGACA AGAAGTACTC CATTGGGCTC GATATCGGCA CAAACAGCGT CGGCTGGGCC
  301 GTCATTACGG ACGAGTACAA GGTGCCGAGC AAAAAATTCA AAGTTCTGGG CAATACCGAT
  361 CGCCACAGCA TAAAGAAGAA CCTCATTGGC GCCCTCCTGT TCGACTCCGG GGAGACGGCC
  421 GAAGCCACGC GGCTCAAAAG AACAGCACGG CGCAGATATA CCCGCAGAAA GAATCGGATC
  481 TGCTACCTGC AGGAGATCTT TAGTAATGAG ATGGCTAAGG TGGATGACTC TTTCTTCCAT
  541 AGGCTGGAGG AGTCCTTTTT GGTGGAGGAG GATAAAAAGC ACGAGCGCCA CCCAATCTTT
  601 GGCAATATCG TGGACGAGGT GGCGTACCAT GAAAAGTACC CAACCATATA TCATCTGAGG
  661 AAGAAGCTTG TAGACAGTAC TGATAAGGCT GACTTGCGGT TGATCTATCT CGCGCTGGCG
  721 CATATGATCA AATTTCGGGG ACACTTCCTC ATCGAGGGGG ACCTGAACCC AGACAACAGC
  781 GATGTCGACA AACTCTTTAT CCAACTGGTT CAGACTTACA ATCAGCTTTT CGAAGAGAAC
  841 CCGATCAACG CATCCGGAGT TGACGCCCAA GCAATCCTGA GCGCTAGGCT GTCCAAATCC
  901 CGGCGGCTCG AAAACCTCAT CGCACAGCTC CCTGGGGAGA AGAAGAACGG GCCCTGTTTG
  961 GGTAAATCTT TATCGCCCTG TCACTCGGGC TGACCCCCCA ACTTTAAATC TAACCTTCGA
 1021 CTGCCGAGAT GGCTAAGCCT TCCAACTGAA GCCAAAGACA CTACGATGAT GGATTCTCGA
1081 CCATCCTGCC TGGGTCA
//
Primer: EGFP-N RDB14657_A7Csb.seq
>D04203A6_A7Cs_1_EGFP-N_F10_16_ABI24.ab1
    1 GTTTACCGGG GGGACAGCTC CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG
   61 TCGCCGGCCT GCTTCAGCAG GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA
  121 GCCCTGCTGT CTCCACCGAG CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAATT
  181 GACTGATGAA TCAGTGTGGC GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTCTGTCT
  241 ATGGTGGTGT CGAAGTACTT GAAGGCTGCA GGCGCGCCCA AGTTGGTCAG AGTAAACAAG
  301 TGGATAATGT TTTCTGCCTG CTCCCTGATG GGCTTATCCC TGTGCTTATT GTAAGCAGAA
  361 AGCACCTTAT CGAGGTTAGC GTCGGCGAGG ATCACTCTTT TGGAGAATTC GCTTATTTGC
  421 TCGATGATCT CATCAAGGTA GTGTTTGTGT TGTTCCACGA ACAGCTGCTT CTGCTCATTA
  481 TCTTCGGGAG ACCCTTTGAG CTTTTCATAG TGGCTGGCCA GATACAAGAA ATTAACGTAT
  541 TTAGAGGGCA GTGCCAGCTC GTTACCTTTC TGCAGCTCGC CCGCACTAGC GAGCATTCGT
  601 TTCCGGCCGT TTTCAAGCTC AAAGAGAGAG TACTTGGGAA GCTTAATGAT GAGGTCTTTT
  661 TTGACCTCTT TATATCCTTT CGCCTCGAGA AAGTCGATGG GGTTTTTTTC GAAGCTTGAT
  721 CGCTCCATGA TTGTGATGCC CAGCAGTTCC TTGACGCTTT TGAGTTTTTT AGACTTCCCT
  781 TTCTCCACTT TGGCCACAAC CAGTACACTG TAAGCGACTG TAGGAGAATC GAATCCGCCG
  841 TATTTCTTGG GGTCCCAATC TTTTTTTGCG TGCGATCAGC TTGTCGCTGT TCCTTTTCGG
  901 GAGGATACTT TCCTTGGAGA AGCCCTCCGG GTCTGTACTT CGGTCTTTTT AACGATGTTC
  961 ACCTGCGGGC ATGGACAGGG ACCCTTCCCG GACTGTCGCG AAATCCCTAC CCTTGTCCCC
 1021 AACACGAATT TCTCTTGTTT CTCCGTTTGT TTCGATAGTT GGATCGGCTT TCGATTCCTC
1081 ATGCCAGTGT AAATCTCGGT CTTGGAAAAA AAT
//
Primer: EGFP-C2 RDB14657_A7Csc.seq
>D04203A6_A7Cs_1_EGFP-C2_G10_19_ABI24.ab1
    1 CTTCCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC
   61 TCTCGGCATG GACGAGCTGT ACAAGTGAAA GGGTTCGATC CCTACCGGTT AGTAATGAGT
  121 TTAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA
  181 TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGAAAAAA AAAAAAAAAA AAAAAAAAAA
  241 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAGGCGCTA
  301 GCTTTAACTT CGATCCCCCC CCGAAACCCA TTGGGGCCAA AACGCCCGCG TTTCTTCCTT
  361 TTCCCCACCC CCCCCCCCAA TTCGGGTGAA GGCCCAGGGC TCGCAACCAA AGTTGGGGGG
  421 GAGGGCCGGC CAAACAAATC GGCACACTGG GGTTCAAGGG GAACCCCCCC GCCCCTTGAC
  481 GGCCATTACC GCGGGGGGGG GGGGTTACCC CACACTGGAC GTCACTTGGC AAGGCCCTAA
  541 CGCCCCCCCC TTTGGTTTCT TCCCTCCTTT CGCCCATTTC CGGGTTTCCG TAAGTTTAAT
  601 CGGGGCCCTT TAGGGTCCAA ATAGGCTTAG GCCTCACCCA AAAATTATAG GGAGGTCCTA
  661 TGGCACTCCC TGAAACGTTC CCTTACTGGA CCCTTTTAAA GGACTGTTCA AGGAAACCCC
  721 ACTCGGCATT TGATAAGGAT TCCATCCCTG GGAAAGCGTT CAATTCCAAT TTGGAGGGCA
781 AAGGGAATCC TTCCCAGAGA TTTGAGGGGT CTCTTACACC CGTTGAGCCC CCCC
//
Primer: SV40pro_F RDB14657_A7Csd.seq
>D04203A6_A7Cs_1_SV40pro_F_H01_08_ABI08.ab1
    1 CTCTGCCCCT GGCCAATCAG AGGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG
  721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT
  781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG
  841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC
  901 GCCTTCTTGA CGAGTTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC
  961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCACGCGCT TTCTATGGAA AGTTGGCCTT
 1021 CGGATTCGGT TTCGGGACGC GGCTGATGGA TTCTCAGCGC GGGAATCTCA ATGCTGAGTC
1081 TCGCCACCTA CTTGGTTATT GCCAGCCA
//

Please visit Sequencing and PCR primers for primer information.


References

References


2018.09.20

GNP_filter3_RDBDEP_html_180823.pl