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pEF-T7-GST-Kif1c-tail-879-1100/M-INK

M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c.

Catalog number RDB14634
Resource name pEF-T7-GST-Kif1c-tail-879-1100/M-INK
Clone info. M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region (amino acid residues 879-1100) of the mouse kinesin superfamily protein, member 1C (Kif1c) under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Please refer Fukuda, M. J. Biol. Chem., 277 (14): 12432-12436, 2002 (PMID 11856727) for pEF-T7-GST vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-T7-GST (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Kif1c (NCBI Gene 16562) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al. J. Biochem., 2017, in press, pii: mvw100. doi: 10.1093/jb/mvw100)
(Japanese text [open/close])

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK DNA solution

Sequence information

References and tips

Electronic file

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References

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J Biochem. 2017 Jan 17. pii: mvw100. doi: 10.1093/jb/mvw100. [Epub ahead of print] PMID 28096452.

2017.10.19

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