RDB14634 - pEF-T7-GST-Kif1c-tail-879-1100/M-INK

Resource data sheet
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pEF-T7-GST-Kif1c-tail-879-1100/M-INK

M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c.


Catalog number	RDB14634
Resource name	pEF-T7-GST-Kif1c-tail-879-1100/M-INK
Clone info.	M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region (amino acid residues 879-1100) of the mouse kinesin superfamily protein, member 1C (Kif1c) under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain. Please refer Fukuda, M. J. Biol. Chem., 277 (14): 12432-12436, 2002 (PMID 11856727) for pEF-T7-GST vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone	pEF-T7-GST (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Kif1c cDNA
Depositor	Fukuda, Mitsunori \|
Other clones in our bank	mouse Kif1c (NCBI Gene 16562) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering Information [in Japanese] [in English]

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
(Japanese text [open/close])

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。

Catalog # Resource name Shipping form Fee (non-profit org.)

RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK DNA solution

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017) (Japanese text [open/close]) 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14634	pEF-T7-GST-Kif1c-tail-879-1100/M-INK	DNA solution

References and tips

Electronic file

Featured content	Mitsunori Fukuda's Rab panel clones (English text)
Featured content	Dnaconda's recommendation EXR0070e (English text)
Featured content	Dnaconda's recommendation EXR0070j (Japanese text)
Featured content	Organelle marker (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB14634_A7C1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F	RDB14634_A7C1a.seq
>D04384A3_A7C1_2_EF1a-C_F_F02_17_ABI24.ab1 1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT 61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT 121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCTATGTCCC 181 CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC CACTCGACTT CTTTTGGAAT 241 ATCTTGAAGA AAAATATGAA GAGCATTTGT ATGAGCGCGA TGAAGGTGAT AAATGGCGAA 301 ACAAAAAGTT TGAATTGGGT TTGGAGTTTC CCAATCTTCC TTATTATATT GATGGTGATG 361 TTAAATTAAC ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC AACATGTTGG 421 GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT TTGGATATTA 481 GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA AACTCTCAAA GTTGATTTTC 541 TTAGCAAGCT ACCTGAAATG CTGAAAATGT TCGAAGATCG TTTATGTCAT AAAACATATT 601 TAAATGGTGA TCATGTAACC CATCCTGACT TCATGTTGTA TGACGCTCTT GATGTTGTTT 661 TATACATGGA CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT AAAAAACGTA 721 TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA GCATGGCCTT 781 TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC 841 CGCGTGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAAGGG 901 CCAGAAGCAG TAGAGGAGAC AGTGCCCAAC GACCACTCAC CAGCTGTCCG GCCCACCTCA 961 CCACCTCTGT CAGCTGGGAG CGGGGTGTCG AGATTGATGA GAGACCTGCA TCGTCGCGCC 1021 GCATCGCTGC TCAGCAGACA GCTGCGCTGC AAGAACTGCA AGTCGCTGAG CCGATGGGTA 1081 ACTGCCAGGC TTCAGCCCGC GCATTTGA //
Primer: hGCSF_R	RDB14634_A7C1b.seq
>D04384A3_A7C1_2_hGCSF_R_E02_14_ABI24.ab1 1 CGGGGGCTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT 61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCACAC AGCTGCCCCA CTCTCTTTGA 121 GGTCAGGGGC TGAGCGCTGC CGTCGCATTC GCGGAGGGGT AGTGTATGGT GGGTAGCGGG 181 GCCCTGGCCG CTGGGCTGGG CTGGGCTGGG GCTGTTGGGG ATAACCGTTG TGCTTTTGGG 241 GCCGTAAGTG CTGGGGTTCT GGCTGTGTAG AACCCCCTCC CCGAGATCGG CTCCCTCCAT 301 CTAGGGAATT CCTGCGAGAA CGGTGGGGTC TTCGGGGACT TGGAGGCCTA CGGTTAGGGG 361 GTGCTGGGGG GACAGTGACC TCTTCAGGGG GCTGAGGAGC TGGGGCCTCT CCACTCCCCA 421 TTCCTGGCCA GGACTCCCTG TGCTGAGGGT TGCTTTTGAA GGGGAAGCGC AGCTTGCAAT 481 CATGCGGGGG CACAAAGCGG GCTGGAGGCC TGCGCAGTCC CCCACCCCGG CCCCCAGCGC 541 CCTGCAGTCC CTGCAGCCGC AGCTGCTCCT GCTTGAGCCA GCGAAGGCGG CCGCGACGGA 601 AGGCAGGGTC CTCTTCCATC AATCTCGACA CCCGCTCCCA GCTGGACAGA GGTGGTGAGG 661 TGGGCCGGAC AGCTGGTGAG TGGTCGTTGG GCACTGTCTC CTCTACTGCT TCTGGCCCTT 721 CCGGTGGGGC CCAGGTGACC AAACCAGACT CGTCATTATC ATCAGATCCA CGCGGAACCA 781 GATCCGATTT TGGAGGATGG TCGCCACCAC CAAACGTGGC TTGCCAGCCC TGCAAAGGCC 841 ATGCTATATA CTTGCTGGAT TTCAAGTACT TATCAATTTG TGGGATAGCT TCAATACGTT 901 TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA 961 ACATCAAGAG CGTCATACAA CATGAAAGTC AGGATGGGTT TACATGATCA CCATTTAAAA 1021 TATGTTTTTA TGACATAAAC GATCTTCGAA CATTTTTCAG CATTTCCAGG TAGCTTGCTA 1081 AGAAAAATCA ACTTTGGAAG GTTCCAAGGT CTTTTTACTA TATGCAAGTT CTTCGGAAAA 1141 ACACCCGTTA TC //
Primer: pGEX-F	RDB14634_A7C1c.seq
>D04384A3_A7C1_2_pGEX_F_D02_11_ABI24.ab1 1 CTTGAAATCC GTCCAAAATC GGGATCTGGT TCCGCGTGGA TCTGATGATA ATGACGAGTC 61 TGGTTTGGTC ACCTGGGCCC CACCGGAAGG GCCAGAAGCA GTAGAGGAGA CAGTGCCCAA 121 CGACCACTCA CCAGCTGTCC GGCCCACCTC ACCACCTCTG TCCAGCTGGG AGCGGGTGTC 181 GAGATTGATG GAAGAGGACC CTGCCTTCCG TCGCGGCCGC CTTCGCTGGC TCAAGCAGGA 241 GCAGCTGCGG CTGCAGGGAC TGCAGGGCGC TGGGGGCCGG GGTGGGGGAC TGCGCAGGCC 301 TCCAGCCCGC TTTGTGCCCC CGCATGATTG CAAGCTGCGC TTCCCCTTCA AAAGCAACCC 361 TCAGCACAGG GAGTCCTGGC CAGGAATGGG GAGTGGAGAG GCCCCAGCTC CTCAGCCCCC 421 TGAAGAGGTC ACTGTCCCCC CAGCACCCCC TAACCGTAGG CCTCCAAGTC CCCGAAGACC 481 CCACCGTTCT CGCAGGAATT CCCTAGATGG AGGGAGCCGA TCTCGGGGAG GGGGTTCTAC 541 ACAGCCAGAA CCCCAGCACT TACGGCCCCA AAAGCACAAC GGTTATCCCC AACAGCCCCA 601 GCCCAGCCCA GCCCAGCGGC CAGGGCCCCG CTACCCACCA TACACTACCC CTCCGCGAAT 661 GCGACGGCAG CGCTCAGCCC CTGACCTCAA AGAGAGTGGG GCAGCTGTGT GAAATCACTA 721 GTGAATTCGC GGCCGCCTGC AGGTCGACCA TAGCGGCCGC TCTAGAGTGA GGGTCCCCAC 781 CTGGGACCCT TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT 841 TTAAACAGCA GTGTTCCCCA TCTGGGGTCC TTGCACCCCT CACTCTGGCC TCAGCCGACT 901 GCACAGCGGC CCCTGCATCC CCTTGGCTGT GAGGCCCCTG GACAAGCAGA GGTGGCCAGA 961 GCTGGGAGGC ATGGCCCTGG GGGTCCCCAC GAATTTGCTG GGGGAAATCT CGTTTTTCTT 1021 CTTAAGACTT TTTGGGGAAC ATGGCTTTGA CCTCCCGGAA CATCCACCGA CGTGTCCTCT 1081 TGTTTTCTGG GGTGGCTCGG GGACACTTGC CTTGCCCCAC GAAGGTCAGA ACTGGTGACC 1141 TCTTTTTTTA AGGGTCA //

Please visit Sequencing and PCR primers for primer information.

References

original	Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

2018.07.12

GNP_filter3_RDBDEP_html_180613.pl