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pEF-T7-GST-Kif1c-tail-879-1100/M-INK (#RDB14634)

M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c.

Clone info. M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region (amino acid residues 879-1100) of the mouse kinesin superfamily protein, member 1C (Kif1c) under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Comment Please refer Fukuda, M. J. Biol. Chem., 277 (14): 12432-12436, 2002 (PMID 11856727) for pEF-T7-GST vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-T7-GST (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-T7-GST-Kif1c-tail-879-1100/M-INK was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14634).

Reference section:

Ishida, M., Marubashi, S., Fukuda, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14634_A7C1p1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1 alpha pro,insert5'(T7 tag,GST,Kif1c 5')
Sequence file: RDB14634_A7C1a.seq check
>D04384A3_A7C1_2_EF1a-C_F_F02_17_ABI24.ab1
    1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCTATGTCCC
  181 CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC CACTCGACTT CTTTTGGAAT
  241 ATCTTGAAGA AAAATATGAA GAGCATTTGT ATGAGCGCGA TGAAGGTGAT AAATGGCGAA
  301 ACAAAAAGTT TGAATTGGGT TTGGAGTTTC CCAATCTTCC TTATTATATT GATGGTGATG
  361 TTAAATTAAC ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC AACATGTTGG
  421 GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT TTGGATATTA
  481 GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA AACTCTCAAA GTTGATTTTC
  541 TTAGCAAGCT ACCTGAAATG CTGAAAATGT TCGAAGATCG TTTATGTCAT AAAACATATT
  601 TAAATGGTGA TCATGTAACC CATCCTGACT TCATGTTGTA TGACGCTCTT GATGTTGTTT
  661 TATACATGGA CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT AAAAAACGTA
  721 TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA GCATGGCCTT
  781 TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC
  841 CGCGTGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAAGGG
  901 CCAGAAGCAG TAGAGGAGAC AGTGCCCAAC GACCACTCAC CAGCTGTCCG GCCCACCTCA
  961 CCACCTCTGT CAGCTGGGAG CGGGGTGTCG AGATTGATGA GAGACCTGCA TCGTCGCGCC
 1021 GCATCGCTGC TCAGCAGACA GCTGCGCTGC AAGAACTGCA AGTCGCTGAG CCGATGGGTA
1081 ACTGCCAGGC TTCAGCCCGC GCATTTGA
//
Primer: hGCSF_R (Pr0031)
Region: hG-CSF p(A),insert3'(Kif1c,GST)
Sequence file: RDB14634_A7C1b.seq check
>D04384A3_A7C1_2_hGCSF_R_E02_14_ABI24.ab1
    1 CGGGGGCTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT
   61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCACAC AGCTGCCCCA CTCTCTTTGA
  121 GGTCAGGGGC TGAGCGCTGC CGTCGCATTC GCGGAGGGGT AGTGTATGGT GGGTAGCGGG
  181 GCCCTGGCCG CTGGGCTGGG CTGGGCTGGG GCTGTTGGGG ATAACCGTTG TGCTTTTGGG
  241 GCCGTAAGTG CTGGGGTTCT GGCTGTGTAG AACCCCCTCC CCGAGATCGG CTCCCTCCAT
  301 CTAGGGAATT CCTGCGAGAA CGGTGGGGTC TTCGGGGACT TGGAGGCCTA CGGTTAGGGG
  361 GTGCTGGGGG GACAGTGACC TCTTCAGGGG GCTGAGGAGC TGGGGCCTCT CCACTCCCCA
  421 TTCCTGGCCA GGACTCCCTG TGCTGAGGGT TGCTTTTGAA GGGGAAGCGC AGCTTGCAAT
  481 CATGCGGGGG CACAAAGCGG GCTGGAGGCC TGCGCAGTCC CCCACCCCGG CCCCCAGCGC
  541 CCTGCAGTCC CTGCAGCCGC AGCTGCTCCT GCTTGAGCCA GCGAAGGCGG CCGCGACGGA
  601 AGGCAGGGTC CTCTTCCATC AATCTCGACA CCCGCTCCCA GCTGGACAGA GGTGGTGAGG
  661 TGGGCCGGAC AGCTGGTGAG TGGTCGTTGG GCACTGTCTC CTCTACTGCT TCTGGCCCTT
  721 CCGGTGGGGC CCAGGTGACC AAACCAGACT CGTCATTATC ATCAGATCCA CGCGGAACCA
  781 GATCCGATTT TGGAGGATGG TCGCCACCAC CAAACGTGGC TTGCCAGCCC TGCAAAGGCC
  841 ATGCTATATA CTTGCTGGAT TTCAAGTACT TATCAATTTG TGGGATAGCT TCAATACGTT
  901 TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA
  961 ACATCAAGAG CGTCATACAA CATGAAAGTC AGGATGGGTT TACATGATCA CCATTTAAAA
 1021 TATGTTTTTA TGACATAAAC GATCTTCGAA CATTTTTCAG CATTTCCAGG TAGCTTGCTA
 1081 AGAAAAATCA ACTTTGGAAG GTTCCAAGGT CTTTTTACTA TATGCAAGTT CTTCGGAAAA
1141 ACACCCGTTA TC
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452. [link to RRC of NBRP]