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pEF-T7-GST-Kif1c-tail-879-1100/M-INK

M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region of the mouse Kif1c.

Catalog number RDB14634
Resource name pEF-T7-GST-Kif1c-tail-879-1100/M-INK
Clone info. M-INK, a novel tool for tagging the mature melanosomes. Expression vector of T7- and GST- tagged C terminal half tail region (amino acid residues 879-1100) of the mouse kinesin superfamily protein, member 1C (Kif1c) under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Please refer Fukuda, M. J. Biol. Chem., 277 (14): 12432-12436, 2002 (PMID 11856727) for pEF-T7-GST vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-T7-GST (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor Fukuda, Mitsunori |
 
Other clones in our bank mouse Kif1c (NCBI Gene 16562) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
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RDB14634 pEF-T7-GST-Kif1c-tail-879-1100/M-INK DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Mitsunori Fukuda's Rab panel clones (English text)
Featured content Dnaconda's recommendation EXR0070e (English text)
Featured content Dnaconda's recommendation EXR0070j (Japanese text)
Featured content Organelle marker (English text)

References

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14634_A7C1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14634_A7C1a.seq
>D04384A3_A7C1_2_EF1a-C_F_F02_17_ABI24.ab1
    1 TCATGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGAGCGG CCGCGGGAAT
  121 TCGATTCCAC CATGGCTAGC ATGACTGGTG GACAGCAAAT GGGTCGCGGA TCTATGTCCC
  181 CTATACTAGG TTATTGGAAA ATTAAGGGCC TTGTGCAACC CACTCGACTT CTTTTGGAAT
  241 ATCTTGAAGA AAAATATGAA GAGCATTTGT ATGAGCGCGA TGAAGGTGAT AAATGGCGAA
  301 ACAAAAAGTT TGAATTGGGT TTGGAGTTTC CCAATCTTCC TTATTATATT GATGGTGATG
  361 TTAAATTAAC ACAGTCTATG GCCATCATAC GTTATATAGC TGACAAGCAC AACATGTTGG
  421 GTGGTTGTCC AAAAGAGCGT GCAGAGATTT CAATGCTTGA AGGAGCGGTT TTGGATATTA
  481 GATACGGTGT TTCGAGAATT GCATATAGTA AAGACTTTGA AACTCTCAAA GTTGATTTTC
  541 TTAGCAAGCT ACCTGAAATG CTGAAAATGT TCGAAGATCG TTTATGTCAT AAAACATATT
  601 TAAATGGTGA TCATGTAACC CATCCTGACT TCATGTTGTA TGACGCTCTT GATGTTGTTT
  661 TATACATGGA CCCAATGTGC CTGGATGCGT TCCCAAAATT AGTTTGTTTT AAAAAACGTA
  721 TTGAAGCTAT CCCACAAATT GATAAGTACT TGAAATCCAG CAAGTATATA GCATGGCCTT
  781 TGCAGGGCTG GCAAGCCACG TTTGGTGGTG GCGACCATCC TCCAAAATCG GATCTGGTTC
  841 CGCGTGGATC TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAAGGG
  901 CCAGAAGCAG TAGAGGAGAC AGTGCCCAAC GACCACTCAC CAGCTGTCCG GCCCACCTCA
  961 CCACCTCTGT CAGCTGGGAG CGGGGTGTCG AGATTGATGA GAGACCTGCA TCGTCGCGCC
 1021 GCATCGCTGC TCAGCAGACA GCTGCGCTGC AAGAACTGCA AGTCGCTGAG CCGATGGGTA
1081 ACTGCCAGGC TTCAGCCCGC GCATTTGA
//
Primer: hGCSF_R RDB14634_A7C1b.seq
>D04384A3_A7C1_2_hGCSF_R_E02_14_ABI24.ab1
    1 CGGGGGCTGA TCTCTCAGGG TCCCAGGTGG GGACCCTCAC TCTAGAGCGG CCGCTATGGT
   61 CGACCTGCAG GCGGCCGCGA ATTCACTAGT GATTTCACAC AGCTGCCCCA CTCTCTTTGA
  121 GGTCAGGGGC TGAGCGCTGC CGTCGCATTC GCGGAGGGGT AGTGTATGGT GGGTAGCGGG
  181 GCCCTGGCCG CTGGGCTGGG CTGGGCTGGG GCTGTTGGGG ATAACCGTTG TGCTTTTGGG
  241 GCCGTAAGTG CTGGGGTTCT GGCTGTGTAG AACCCCCTCC CCGAGATCGG CTCCCTCCAT
  301 CTAGGGAATT CCTGCGAGAA CGGTGGGGTC TTCGGGGACT TGGAGGCCTA CGGTTAGGGG
  361 GTGCTGGGGG GACAGTGACC TCTTCAGGGG GCTGAGGAGC TGGGGCCTCT CCACTCCCCA
  421 TTCCTGGCCA GGACTCCCTG TGCTGAGGGT TGCTTTTGAA GGGGAAGCGC AGCTTGCAAT
  481 CATGCGGGGG CACAAAGCGG GCTGGAGGCC TGCGCAGTCC CCCACCCCGG CCCCCAGCGC
  541 CCTGCAGTCC CTGCAGCCGC AGCTGCTCCT GCTTGAGCCA GCGAAGGCGG CCGCGACGGA
  601 AGGCAGGGTC CTCTTCCATC AATCTCGACA CCCGCTCCCA GCTGGACAGA GGTGGTGAGG
  661 TGGGCCGGAC AGCTGGTGAG TGGTCGTTGG GCACTGTCTC CTCTACTGCT TCTGGCCCTT
  721 CCGGTGGGGC CCAGGTGACC AAACCAGACT CGTCATTATC ATCAGATCCA CGCGGAACCA
  781 GATCCGATTT TGGAGGATGG TCGCCACCAC CAAACGTGGC TTGCCAGCCC TGCAAAGGCC
  841 ATGCTATATA CTTGCTGGAT TTCAAGTACT TATCAATTTG TGGGATAGCT TCAATACGTT
  901 TTTTTAAAAC AAACTAATTT TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA
  961 ACATCAAGAG CGTCATACAA CATGAAAGTC AGGATGGGTT TACATGATCA CCATTTAAAA
 1021 TATGTTTTTA TGACATAAAC GATCTTCGAA CATTTTTCAG CATTTCCAGG TAGCTTGCTA
 1081 AGAAAAATCA ACTTTGGAAG GTTCCAAGGT CTTTTTACTA TATGCAAGTT CTTCGGAAAA
1141 ACACCCGTTA TC
//
Primer: pGEX-F RDB14634_A7C1c.seq
>D04384A3_A7C1_2_pGEX_F_D02_11_ABI24.ab1
    1 CTTGAAATCC GTCCAAAATC GGGATCTGGT TCCGCGTGGA TCTGATGATA ATGACGAGTC
   61 TGGTTTGGTC ACCTGGGCCC CACCGGAAGG GCCAGAAGCA GTAGAGGAGA CAGTGCCCAA
  121 CGACCACTCA CCAGCTGTCC GGCCCACCTC ACCACCTCTG TCCAGCTGGG AGCGGGTGTC
  181 GAGATTGATG GAAGAGGACC CTGCCTTCCG TCGCGGCCGC CTTCGCTGGC TCAAGCAGGA
  241 GCAGCTGCGG CTGCAGGGAC TGCAGGGCGC TGGGGGCCGG GGTGGGGGAC TGCGCAGGCC
  301 TCCAGCCCGC TTTGTGCCCC CGCATGATTG CAAGCTGCGC TTCCCCTTCA AAAGCAACCC
  361 TCAGCACAGG GAGTCCTGGC CAGGAATGGG GAGTGGAGAG GCCCCAGCTC CTCAGCCCCC
  421 TGAAGAGGTC ACTGTCCCCC CAGCACCCCC TAACCGTAGG CCTCCAAGTC CCCGAAGACC
  481 CCACCGTTCT CGCAGGAATT CCCTAGATGG AGGGAGCCGA TCTCGGGGAG GGGGTTCTAC
  541 ACAGCCAGAA CCCCAGCACT TACGGCCCCA AAAGCACAAC GGTTATCCCC AACAGCCCCA
  601 GCCCAGCCCA GCCCAGCGGC CAGGGCCCCG CTACCCACCA TACACTACCC CTCCGCGAAT
  661 GCGACGGCAG CGCTCAGCCC CTGACCTCAA AGAGAGTGGG GCAGCTGTGT GAAATCACTA
  721 GTGAATTCGC GGCCGCCTGC AGGTCGACCA TAGCGGCCGC TCTAGAGTGA GGGTCCCCAC
  781 CTGGGACCCT TGAGAGTATC AGGTCTCCCA CGTGGGAGAC AAGAAATCCC TGTTTAATAT
  841 TTAAACAGCA GTGTTCCCCA TCTGGGGTCC TTGCACCCCT CACTCTGGCC TCAGCCGACT
  901 GCACAGCGGC CCCTGCATCC CCTTGGCTGT GAGGCCCCTG GACAAGCAGA GGTGGCCAGA
  961 GCTGGGAGGC ATGGCCCTGG GGGTCCCCAC GAATTTGCTG GGGGAAATCT CGTTTTTCTT
 1021 CTTAAGACTT TTTGGGGAAC ATGGCTTTGA CCTCCCGGAA CATCCACCGA CGTGTCCTCT
 1081 TGTTTTCTGG GGTGGCTCGG GGACACTTGC CTTGCCCCAC GAAGGTCAGA ACTGGTGACC
1141 TCTTTTTTTA AGGGTCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

2018.09.20

GNP_filter3_RDBDEP_html_180823.pl