Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

G-CaMP7.09

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.09.

Catalog number RDB14612
Resource name G-CaMP7.09
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7.09 is an enhanced G-CaMP for expressing sufficient fluorescence in iPS cell-derived cardiomyocytes (iPSC-CMs).
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Shiba, Y. et al. Nature 538 (7625): 388-391, 2016) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
        Ordering Please visit Information of Request for Distribution.[link] 
        Order form 
        [Credit Card Payment]  [Bank Transfer Payment]
        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Shiba, Y. et al. Nature 538 (7625): 388-391, 2016)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14612 G-CaMP7.09 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14612_A7Bgp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV_Forward
        Region: CMV pro, insert 5'
        Sequence file: RDB14612_A7Bga.seq check
        >D04021A7_A7Bg_1_CMV-Forward_G10_19_ABI24.ab1
            1 TTGGAACACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
           61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC
          121 CACCATGCGG GGTTCTCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA
          181 AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC
          241 ACGTCGTAAG TGGAATAAGA CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT
          301 CGAGAACGTC TATATCAAGG CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT
          361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC
          421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT
          481 TTCGAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
          541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG
          601 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
          661 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT
          721 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
          781 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
          841 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CATCCAGGAG CGCACCATCT TCTTCAAGGA
          901 CGACGGCTAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
          961 CATCGTGCTG AAGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGGC ACAAGCTGGA
         1021 AGTACACACG CGTGACCCAA CTGACTGAGA GCAGATCGCA GATTTTAAGG AAGCTTTCTC
         1081 CCTATTTGAC AGACTGGATG GACCATTACA ACCACGAGCT GGGGGACGAT GAATGGGCGG
        1141 ATCT
        //
        Primer: SV40polyA-R
        Region: insert 3'
        Sequence file: RDB14612_A7Bgb.seq check
        >D04021A7_A7Bg_1_SV40polyA-R_G11_20_ABI24.ab1
            1 AGAAATTGGG AGTTTTAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG
           61 AGTCGCGGCC GCTCACTTCG CTGTCATCAT TTGTACAAAC TCTTCGTAGT TTACCTGACC
          121 ATCCCCATCG ATGTCTGCTT CCCTGATCAT TTCATCAACC TCTTCATCTG TTAACTTCTC
          181 TCCAAGGTTT GTCATCACGT GGCGAAGCTC TGCTGCACTG ATGTAGCCAT TGCCATCCTT
          241 ATCAAACACA CGGAACGCTT CTCTAATTTC TTCTTCACTG TCTGTGTATT TCATTTTTCT
          301 TGCCATCATT GTCAGGAACT CAGGGAAGTC GATTGTGCCA TCACCGTCGG CATCTACTTC
          361 ATTGATCATG TCCTGCAGCT CTGCTTCTGT GGGGTTCTGC CCCAGAGACC GCATCACCGT
          421 CCCCAGCTCC TTGGTTGTTA TTGTCCCATC CCCGTCCTTG TCAAATAGGG AGAAAGCCTC
          481 TTTAAATTCT GCGATCTGCT CTTCAGTCAG TTGGTCACGC GTGTTGTACT CCAGCTTGTG
          541 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCACGATGC GGTTCACCAG
          601 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TAGCCGTCGT CCTTGAAGAA
          661 GATGGTGCGC TCCTGGATGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT
          721 CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT
          781 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA
          841 GTGGCATCGC CCTCACCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC
          901 AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTC
          961 CCTCCGGTAC CGCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGG CGGCGGGTCA
         1021 CGAACTCCAG CAGGACCATG TGATCGCGCT TTCTCGTTGG GGTTCTTTTC GAAAGTTTGG
         1081 ACTGCACGCC TCAGTAGTGA TTGTTCGGGC AGGCAGCACG GGTCGTCGTC GATGGGGATG
        1141 TTTTCTGCTG CTAGTGGTTA GGCGGGAGCC TGTGCACAG
        //
        Primer: SV40pro_F_V2
        Region: NeoR_KanR
        Sequence file: RDB14612_A7Bgc.seq check
        >D04021A4_A7Bg_1_SV40pro_F_V2_D12_12_ABI24.ab1
            1 AACAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
           61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
          121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
          181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
          241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
          301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
          361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
          421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
          481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
          541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
          601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
          661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
          721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
          781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
          841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
          901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
          961 GAATCGTTTT CCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
         1021 AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA TACCCGGAGA
         1081 CCCGCGCTAT GACGGGCAAT AAAGACGGAA TTAAACGGCA CGGATGATGG GATCGTTTTG
        1141 TTCCATATAA AACCGCCCGA G
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl