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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

G-CaMP7.09

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.09.

Catalog number RDB14612
Resource name G-CaMP7.09
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7.09 is an enhanced G-CaMP for expressing sufficient fluorescence in iPS cell-derived cardiomyocytes (iPSC-CMs).
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Shiba, Y. et al. Nature 538 (7625): 388-391, 2016) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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Schedule A (GFP-A) [Link]
Remarks
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RDB14612 G-CaMP7.09 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14612_A7Bgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB14612_A7Bga.seq check
>D04021A7_A7Bg_1_CMV-Forward_G10_19_ABI24.ab1
    1 TTGGAACACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC
  121 CACCATGCGG GGTTCTCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA
  181 AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC
  241 ACGTCGTAAG TGGAATAAGA CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT
  301 CGAGAACGTC TATATCAAGG CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT
  361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC
  421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT
  481 TTCGAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
  541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG
  601 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  661 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT
  721 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  781 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  841 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CATCCAGGAG CGCACCATCT TCTTCAAGGA
  901 CGACGGCTAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  961 CATCGTGCTG AAGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGGC ACAAGCTGGA
 1021 AGTACACACG CGTGACCCAA CTGACTGAGA GCAGATCGCA GATTTTAAGG AAGCTTTCTC
 1081 CCTATTTGAC AGACTGGATG GACCATTACA ACCACGAGCT GGGGGACGAT GAATGGGCGG
1141 ATCT
//
Primer: SV40polyA-R
Region: insert 3'
Sequence file: RDB14612_A7Bgb.seq check
>D04021A7_A7Bg_1_SV40polyA-R_G11_20_ABI24.ab1
    1 AGAAATTGGG AGTTTTAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG
   61 AGTCGCGGCC GCTCACTTCG CTGTCATCAT TTGTACAAAC TCTTCGTAGT TTACCTGACC
  121 ATCCCCATCG ATGTCTGCTT CCCTGATCAT TTCATCAACC TCTTCATCTG TTAACTTCTC
  181 TCCAAGGTTT GTCATCACGT GGCGAAGCTC TGCTGCACTG ATGTAGCCAT TGCCATCCTT
  241 ATCAAACACA CGGAACGCTT CTCTAATTTC TTCTTCACTG TCTGTGTATT TCATTTTTCT
  301 TGCCATCATT GTCAGGAACT CAGGGAAGTC GATTGTGCCA TCACCGTCGG CATCTACTTC
  361 ATTGATCATG TCCTGCAGCT CTGCTTCTGT GGGGTTCTGC CCCAGAGACC GCATCACCGT
  421 CCCCAGCTCC TTGGTTGTTA TTGTCCCATC CCCGTCCTTG TCAAATAGGG AGAAAGCCTC
  481 TTTAAATTCT GCGATCTGCT CTTCAGTCAG TTGGTCACGC GTGTTGTACT CCAGCTTGTG
  541 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCACGATGC GGTTCACCAG
  601 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TAGCCGTCGT CCTTGAAGAA
  661 GATGGTGCGC TCCTGGATGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT
  721 CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT
  781 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA
  841 GTGGCATCGC CCTCACCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC
  901 AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTC
  961 CCTCCGGTAC CGCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGG CGGCGGGTCA
 1021 CGAACTCCAG CAGGACCATG TGATCGCGCT TTCTCGTTGG GGTTCTTTTC GAAAGTTTGG
 1081 ACTGCACGCC TCAGTAGTGA TTGTTCGGGC AGGCAGCACG GGTCGTCGTC GATGGGGATG
1141 TTTTCTGCTG CTAGTGGTTA GGCGGGAGCC TGTGCACAG
//
Primer: SV40pro_F_V2
Region: NeoR_KanR
Sequence file: RDB14612_A7Bgc.seq check
>D04021A4_A7Bg_1_SV40pro_F_V2_D12_12_ABI24.ab1
    1 AACAAACTTT TTTGGAGGCT AGGCTTTTGC AAGATCGATC AAGAGACAGG ATGAGGATCG
   61 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
  121 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
  181 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
  241 GAACTGCAAG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
  301 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
  361 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
  421 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
  481 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
  541 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG
  601 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
  661 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
  721 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
  781 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
  841 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC
  901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
  961 GAATCGTTTT CCCGGGACGC CGGCTGGATG ATCCCTCCAG CGCGGGGATC TCATGCTGGG
 1021 AGTTCTTCGC CCACCCTAGG GGGAGGCTAA CTGAAACACG GAAGGAGACA TACCCGGAGA
 1081 CCCGCGCTAT GACGGGCAAT AAAGACGGAA TTAAACGGCA CGGATGATGG GATCGTTTTG
1141 TTCCATATAA AACCGCCCGA G
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

2020.10.02

GNP_filter3_RDBDEP_html_200316.pl