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G-CaMP7.09

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP7.09.

Catalog number RDB14612
Resource name G-CaMP7.09
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP7.09 is an enhanced G-CaMP for expressing sufficient fluorescence in iPS cell-derived cardiomyocytes (iPSC-CMs).
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Shiba, Y. et al. Nature 538 (7625): 388-391, 2016) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Please use Schedule A (GFP-A) additionally.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14612 G-CaMP7.09 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

References

original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14612_A7Bgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14612_A7Bga.seq
>D04021A7_A7Bg_1_CMV-Forward_G10_19_ABI24.ab1
    1 TTGGAACACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGACT CAGATCTCGC
  121 CACCATGCGG GGTTCTCATC ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA
  181 AATGGGTCGG GATCTGTACG ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC
  241 ACGTCGTAAG TGGAATAAGA CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT
  301 CGAGAACGTC TATATCAAGG CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT
  361 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC
  421 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT
  481 TTCGAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
  541 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG
  601 CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT
  661 AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT
  721 GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA CCCTCGTGAC
  781 CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA AGCAGCACGA
  841 CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CATCCAGGAG CGCACCATCT TCTTCAAGGA
  901 CGACGGCTAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC TGGTGAACCG
  961 CATCGTGCTG AAGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGGC ACAAGCTGGA
 1021 AGTACACACG CGTGACCCAA CTGACTGAGA GCAGATCGCA GATTTTAAGG AAGCTTTCTC
 1081 CCTATTTGAC AGACTGGATG GACCATTACA ACCACGAGCT GGGGGACGAT GAATGGGCGG
1141 ATCT
//
Primer: SV40polyA-R RDB14612_A7Bgb.seq
>D04021A7_A7Bg_1_SV40polyA-R_G11_20_ABI24.ab1
    1 AGAAATTGGG AGTTTTAGCA GTAAACCTCT ACAAATGTGG TATGGCTGAT TATGATCTAG
   61 AGTCGCGGCC GCTCACTTCG CTGTCATCAT TTGTACAAAC TCTTCGTAGT TTACCTGACC
  121 ATCCCCATCG ATGTCTGCTT CCCTGATCAT TTCATCAACC TCTTCATCTG TTAACTTCTC
  181 TCCAAGGTTT GTCATCACGT GGCGAAGCTC TGCTGCACTG ATGTAGCCAT TGCCATCCTT
  241 ATCAAACACA CGGAACGCTT CTCTAATTTC TTCTTCACTG TCTGTGTATT TCATTTTTCT
  301 TGCCATCATT GTCAGGAACT CAGGGAAGTC GATTGTGCCA TCACCGTCGG CATCTACTTC
  361 ATTGATCATG TCCTGCAGCT CTGCTTCTGT GGGGTTCTGC CCCAGAGACC GCATCACCGT
  421 CCCCAGCTCC TTGGTTGTTA TTGTCCCATC CCCGTCCTTG TCAAATAGGG AGAAAGCCTC
  481 TTTAAATTCT GCGATCTGCT CTTCAGTCAG TTGGTCACGC GTGTTGTACT CCAGCTTGTG
  541 CCCCAGGATG TTGCCGTCCT CCTTGAAGTC GATGCCCTTC AGCACGATGC GGTTCACCAG
  601 GGTGTCGCCC TCGAACTTCA CCTCGGCGCG GGTCTTGTAG TAGCCGTCGT CCTTGAAGAA
  661 GATGGTGCGC TCCTGGATGT AGCCTTCGGG CATGGCGGAC TTGAAGAAGT CGTGCTGCTT
  721 CATGTGGTCG GGGTAGCGGC TGAAGCACTG CACGCCGTAG GTCAGGGTGG TCACGAGGGT
  781 GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTCAGGGTCA GCTTGCCGTA
  841 GTGGCATCGC CCTCACCCTC GCCGGACACG CTGAACTTGT GGCCGTTTAC GTCGCCGTCC
  901 AGCTCGACCA GGATGGGCAC CACCCCGGTG AACAGCTCCT CGCCCTTGCT CACCATGCTC
  961 CCTCCGGTAC CGCCCTTGTA CAGCTCGTCC ATGCCGAGAG TGATCCCGGG CGGCGGGTCA
 1021 CGAACTCCAG CAGGACCATG TGATCGCGCT TTCTCGTTGG GGTTCTTTTC GAAAGTTTGG
 1081 ACTGCACGCC TCAGTAGTGA TTGTTCGGGC AGGCAGCACG GGTCGTCGTC GATGGGGATG
1141 TTTTCTGCTG CTAGTGGTTA GGCGGGAGCC TGTGCACAG
//
Primer: SV40pro_F_V2 RDB14612_A7Bgc.seq
>D04021A7_A7Bg_1_SV40pro_F_V2_G12_21_ABI24.ab1
    1 ATAGAGCCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG ATCAAGAGAC AGGATGAGGA
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  961 TCGGAATTCG TTTTCCCGGG ACGCCGGCTG GGATGATCCT CCAGCGCGGG GATCTCATGC
 1021 TGGAGTTCTT CGCCCACCCC TAGGGGGAGG CTAACTGAAC ACGGAGGAGA CATTACTGGA
 1081 GACCGCGCTA TGACGCAATT AAGGACGGAA TTAAACGCAC GGTGTTTGGG GATCGGTTTG
1141 ATTTCCAATA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Shiba, Y., Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts. Nature 538 (7625): 388-391 (2016). PMID 27723741.

2018.09.20

GNP_filter3_RDBDEP_html_180823.pl