Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

R-CaMP1.07

Genetically-encoded fluorescent calcium sensor cDNA, R-CaMP1.07.

Catalog number RDB14608
Resource name R-CaMP1.07
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of mApple, calmodulin, and the M13 peptide from myosin light chain kinase. By combining this red fluorescent calcium sensor with the green fluorescent calcium sensor, such as G-CaMPs became possible to observe the relationship between two different neural networks.
Coding sequence of EGFP was replaced with R-CaMP1.07 sequence. Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Discosoma sp. RFP cDNA
Reference of nucleotide sequence AKE44627.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (7): e39933, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
Ordering Please visit Information of Request for Distribution.[link] 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose)[Link]
Remarks
The BIOLOGICAL RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. Please read the Exclusive MTA (Takara Bio USA, Inc. FPs, for use for not-for-profit academic purpose).
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14608 R-CaMP1.07 DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14608_A7Bgp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14608_A7Bga.seq check
>D04021A3_A7Bg_1_CMV-Forward_C10_07_ABI24.ab1
    1 TGGAACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGCCA
  121 CCATGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
  181 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC
  241 GTCGTAAGTG GAATAAGGCC GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACCCG
  301 TGGTGTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GAGCGAGATC AAGAAGGGCC
  361 TGCGCCTGAA GGACGGCGGC CACTACGCCG CCGAGGTCAA GACCACCTAC AAGGCCAAGA
  421 AGCCCGTGCA GCTGCCCGGC GCCTACATCG TGGACATCAA GTTGGACATC GTGTCCCACA
  481 ACGAGGACTA CACCATCGTG GAACAGTGCG AACGCGCCGA GGGCCGCCAC TCCACCGGCG
  541 GCATGGACGA GCTGTACAAG GGCGGTACCG GAGGGAGCCT GGTGAGCAAG GGCGAGGAGG
  601 ACAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA
  661 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GCCTTTCAGA
  721 CCGCTAAGCT TGTCGTCGTT AAGGGTGGTC CTCTGCCCTT CGCCTGGGAC ATCCTGTCCC
  781 CTCAGTTCAT GTACGGCTCC AAGGCCTACA TTAAGCACCC AGCCGACATC CCCGACTACT
  841 TCAAGCTGTC CTTCCCCGAG GGCTTCAGGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
  901 GCATTATTCA CGTTAACCAG GACTCCTCCC TGCAAGACGG CGTGTTCATC TACAAGGTGA
  961 AGCTGCGCGG CACCAACTTC CCCCCCCGAC GGCCCCGTAA TGCAGAGAGA CCATGGCTGG
 1021 GAGGCCACGC GTGACCACTG ACTGAGAGCA GATCGCAGAT TAAGAGCCTT TCTCCTATTT
1081 GACAGACGGC ATGGACATTA CCACAACGAA GCTGGGGGAC GGGTTAT
//
Primer: SV40polyA-R
Sequence file: RDB14608_A7Bgb.seq check
>D04021A3_A7Bg_1_SV40polyA-R_C11_08_ABI24.ab1
    1 GAGGAGGGGA GTTTTAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
   61 AGAGTCGCGG CCGCTATGGG TTGGACTCCA CGTCTCCCGC CAACTTGAGA AGGTCAAAAT
  121 TCAAAGTCTG TTTCACCGGG AATTCACCAC CACCACCACC ACCACTGCCT CCAGTCCCTC
  181 CGCCCTTCGC TGTCATCATT TGTACAAACT CTTCGTAGTT TACCTGACCA TCCCCATCGA
  241 TGTCTGCCAC CCTGATCATT TCATCAACCT CTTCATCTGT TAACTTCTCT CCAAGGTCTG
  301 TCATCACGTG GCGAAGCTCT GCTGCGCCGA TGTAGCCATT GCCATCCTTA TCAAACACAC
  361 GGAACGCTTC TCTAATTTCT TCTTCACTGT CTGTGTCGTT CATTTTTCTT GCCATCATTG
  421 TCAGGAACTC AGGGAAGTCG AATGTGCCAT CACCGTCGGC ATCTACTTCA TTGATCATGT
  481 CCTGCAGCTC TGCTTCTGTG GGGTTCTGCC CCAGAGACCG CATCACCGTC CCCAGCTCCT
  541 TGGTTGTTAT TGTCCCATCC CCGTCCTTGT CAAATAGGGA GAAAGCCTCT TTAAATTCTG
  601 CGATCTGCTC TTCAGTCAGT TGGTCACGCG TGGCCTCCCA GCCCATGGTC TTCTTCTGCA
  661 TTACGGGGCC GTCGGGGGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACACGC
  721 CGTCTTGCAG GGAGGAGTCC TGGTTAACGT GAATAATGCC GCCGTCCTCG AAGTTCATCA
  781 CGCGCTCCCA CCTGAAGCCC TCGGGGAAGG ACAGCTTGAA GTAGTCGGGG ATGTCGGCTG
  841 GGTGCTTAAT GTAGGCCTTG GAGCCGTACA TGAACTGAGG GGACAGGATG TCCCAGGCGA
  901 AAGGGCAGAG GACCACCCTT AACGACGACA AGCTTAGCGG TCTGAAAGGC CTCGTAGGGG
  961 CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGTGC
 1021 ACCTTGAGCG CATGACTCCT TGATGATGGC ATGTGTCTCT CGCCTGCTCA CAGCTCCTCG
 1081 TACGCCTGTA CAGCTCGTCA TGCCGCGTGA ATGCCGTCCT CGGCGCGGTT CGCCACTGTT
1141 TTCCG
//
Primer: SV40pro_F_V2
Sequence file: RDB14608_A7Bgc.seq check
>D04021A3_A7Bg_1_SV40pro_F_V2_C12_09_ABI24.ab1
    1 AGCAGAGCTT TTTTTGGAAG CCTAGGCTTT TGCAAGATCG ATCAAGAGAC AGGATGAGGA
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
  781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
  901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
  961 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GATGATCCTC CCAGCGCGGG GGATCTCATG
 1021 CTGGAGTTCT TCGCCCCACC CCTAAGGGGG AGGCTACTGA ACACGGAAGA GACAATTACT
 1081 GATGACCGCG CTATGACGGC ATAAGACCGA TTAACGCACG ATGTGGGTCG TTTTGGTTTC
1141 CATAAAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.
reference Bethge, P., An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator. PLoS One 12 (6): e0179460 (2017). PMID 28640817.
reference Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959.

2021.03.11

GNP_filter3_RDBDEP_html_210308.pl