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R-CaMP1.07 (#RDB14608)

Genetically-encoded fluorescent calcium sensor cDNA, R-CaMP1.07.

Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of mApple, calmodulin, and the M13 peptide from myosin light chain kinase. By combining this red fluorescent calcium sensor with the green fluorescent calcium sensor, such as G-CaMPs became possible to observe the relationship between two different neural networks.
Comment Coding sequence of EGFP was replaced with R-CaMP1.07 sequence. Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AKE44627.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
 

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Terms and conditions for distribution In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (7): e39933, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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MTAに書く使用条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (7): e39933, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。

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RDB14608 R-CaMP1.07 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The R-CaMP1.07 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14608).

Reference section:

Ohkura, M., Sasaki, T., Kobayashi, C., Ikegaya, Y., Nakai, J., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14608_A7Bgp1-3.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro,insert 5'
Sequence file: RDB14608_A7Bgd.seq check
>D04021A3_A7Bg_1_CMV-Forward_C10_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
   61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGCCA
  121 CCATGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
  181 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC
  241 GTCGTAAGTG GAATAAGGCC GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACCCG
  301 TGGTGTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GAGCGAGATC AAGAAGGGCC
  361 TGCGCCTGAA GGACGGCGGC CACTACGCCG CCGAGGTCAA GACCACCTAC AAGGCCAAGA
  421 AGCCCGTGCA GCTGCCCGGC GCCTACATCG TGGACATCAA GTTGGACATC GTGTCCCACA
  481 ACGAGGACTA CACCATCGTG GAACAGTGCG AACGCGCCGA GGGCCGCCAC TCCACCGGCG
  541 GCATGGACGA GCTGTACAAG GGCGGTACCG GAGGGAGCCT GGTGAGCAAG GGCGAGGAGG
  601 ACAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA
  661 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAN GCCTTTCAGA
  721 CCGCTAAGCT TGTCGTCGTT AANGGTGGTC CTCTGCCCTT CGCCTGGGAN ATCCTGTCCC
  781 CTCAGTTCAT GTACGGCTCC NANGNCTACA TTAAGCACCC AGCCGANATC CCCGANTACT
  841 TCNAGCTGTC CTTCCCCGAN GGCTTCNNGT GGGANCGCGN GATGAACTNC NANGANNGNG
  901 GNNTTATTNN CGTNNANCAN NNNNNNNNCN NGNNNGANNG NGNGNNNNTC NNNNNNNNGA
961 AGCNGNNNNN NNNNN
//
Primer: SV40polyA-R (Pr0109)
Region: insert 3'
Sequence file: RDB14608_A7Bge.seq check
>D04021A3_A7Bg_1_SV40polyA-R_C11_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNAGNN NGTANANCCT CTANNAATGT GGTATGGCTG ATTATGATCT
   61 AGAGTCGCGG CCGCTATGGG TTGGACTCCA CGTCTCCCGC CAACTTGAGA AGGTCAAAAT
  121 TCAAAGTCTG TTTCACCGGG AATTCACCAC CACCACCACC ACCACTGCCT CCAGTCCCTC
  181 CGCCCTTCGC TGTCATCATT TGTACAAACT CTTCGTAGTT TACCTGACCA TCCCCATCGA
  241 TGTCTGCCAC CCTGATCATT TCATCAACCT CTTCATCTGT TAACTTCTCT CCAAGGTCTG
  301 TCATCACGTG GCGAAGCTCT GCTGCGCCGA TGTAGCCATT GCCATCCTTA TCAAACACAC
  361 GGAACGCTTC TCTAATTTCT TCTTCACTGT CTGTGTCGTT CATTTTTCTT GCCATCATTG
  421 TCAGGAACTC AGGGAAGTCG AATGTGCCAT CACCGTCGGC ATCTACTTCA TTGATCATGT
  481 CCTGCAGCTC TGCTTCTGTG GGGTTCTGCC CCAGAGACCG CATCACCGTC CCCAGCTCCT
  541 TGGTTGTTAT TGTCCCATCC CCGTCCTTGT CAAATAGGGA GAAAGCCTCT TTAAATTCTG
  601 CGATCTGCTC TTCAGTCAGT TGGTCACGCG TGGCCTCCCA GCCCATGGTC TTCTTCTGCA
  661 TTACGGGGCC GTCGGGGGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACACGC
  721 CGTCTTGCAN GGAGGANTCC TGNTTAACGT GAATAATGCC GCCGTCCTCG AAGTTCATCA
  781 CGCGCTCCCA CCTGAAGCCN TCGGGGAANG ANAGCTTGAA GTANTCGGGG ANGTCGGCTG
  841 GGTGCTTAAT GTANNNCTNG NANCCGNANN TGAACTGANG GGNNANGANG TCCCANGNGA
901 ANNGNCANAN GANNACCCTT NNCGACNANN NNCTTANCGG NCTGAANNNN NNNNNN
//
Primer: Red-C_F (Pr0432)
Region: insert mid
Sequence file: RDB14608_A7Bgf.seq check
>14608_14608_B3GrF_Red-C_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCCCN CAACNNNGNN TACNCNNTCN NGGAACAGTG
   61 CGAACGCGCC GANGGCCGCC ACTCCACCGG CGGCATGGNC GAGCTGTACA AGGGCGGTAC
  121 CGGNNGGAGC CTGGTGAGCA AGGGCGAGGA GGACAACATG GCCATCATCA AGGAGTTCAT
  181 GCGCTTCAAG GTGCACATGG AGGGCTCCGT GAACGGCCAC GAGTTCGAGA TCGAGGGCGA
  241 GGGCGAGGGC CGCCCCTACG AGGCCTTTCA GACCGCTAAG CTTGTCGTCG TTAAGGGTGG
  301 TCCTCTGCCC TTCGCCTGGG ACATCCTGTC CCCTCAGTTC ATGTACGGCT CCAAGGCCTA
  361 CATTAAGCAC CCAGCCGACA TCCCCGACTA CTTCAAGCTG TCCTTCCCCG AGGGCTTCAG
  421 GTGGGAGCGC GTGATGAACT TCGAGGACGG CGGCATTATT CACGTTAACC AGGACTCCTC
  481 CCTGCAAGAC GGCGTGTTCA TCTACAAGGT GAAGCTGCGC GGCACCAACT TCCCCCCCGA
  541 CGGCCCCGTA ATGCAGAAGA AGACCATGGG CTGGGAGGCC ACGCGTGNCC AACTGACTGA
  601 AGAGCAGATC GCANAATTTA AAGAGGCTTT CTCCCTATTT GACAAGGACG GGGATGGGAC
  661 AATAACAACC NAGGAGCTGG GGACGGTGNN GCGGTCTCTG GGGCANAANC CNCACANANN
  721 CAGANCTGCA NGANATGATC NATGAANTAN ANGNCGACGG TGNTGGCACA TTCNANTTCC
  781 CTGANTTCCT GANNNTGATN GCNAGAAAAA TGANCGANNC ANNNNGNGAA NANNAANTTA
841 NANAAGCNTT NCNNGNGTTT NNTNANGATG GNNNNNNNNN N
//
Primer: SV40pro_F_V2 (Pr0154)
Region: NeoR_KanR
Sequence file: RDB14608_A7Bgg.seq check
>D04021A3_A7Bg_1_SV40pro_F_V2_C12_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTT TGCAAGNNCG ATCNAGAGAC AGGATGAGGA
   61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
  121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
  181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
  241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
  301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
  361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
  421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
  481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
  541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
  601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
  661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
  721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGNGCT
  781 GANCGCTTCC TCGNGCTTTA CGGTATCGCC GCTCCNGATT CGCAGCGCAT CGCCTTCTAT
  841 CGCCTTCTTG ANNANTTCTT CTGANCGGGN NTCTGGGGTT CGAANTGANN NANCNAGCGA
  901 CGCCCNACCT GCCNTCACGA GATTTCGATT NCACCGCCNC CTTCTATGAA NNNNNGGGNT
  961 TNNNNNNNGT TTNNCNNGNN NNNCNNNNNG GNTGNNNNNN CNNNNNNNNG GNNNNNNNNN
1021 N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076. [link to RRC of NBRP]
reference Bethge, P., An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator. PLoS One 12 (6): e0179460 (2017). PMID 28640817.
reference Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959.