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R-CaMP1.07

Genetically-encoded fluorescent calcium sensor cDNA, R-CaMP1.07.

Catalog number RDB14608
Resource name R-CaMP1.07
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of mApple, calmodulin, and the M13 peptide from myosin light chain kinase. By combining this red fluorescent calcium sensor with the green fluorescent calcium sensor, such as G-CaMPs became possible to observe the relationship between two different neural networks.
Comment Coding sequence of EGFP was replaced with R-CaMP1.07 sequence. Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Discosoma sp. RFP cDNA
Reference of insert sequence AKE44627.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
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External Database
Rat Calm2 Chickin MLCK Discosoma sp. RFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Ohkura, M. et al. PLos One 7 (7): e39933, 2012) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
        Remarks
        This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Ohkura, M. et al. PLos One 7 (7): e39933, 2012)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
        備考
        Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

        Catalog # Resource name Shipping form Fee (non-profit org.)
        RDB14608 R-CaMP1.07 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        References and tips

        Electronic file

        Original reference

        original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB14608_A7Bgp1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: CMV-Forward
        Sequence file: RDB14608_A7Bga.seq check
        >D04021A3_A7Bg_1_CMV-Forward_C10_07_ABI24.ab1
            1 TGGAACACTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
           61 AGCAGAGCTG GTTTAGTGAA CCGTCAGATC CGCTAGCGCT ACCGGACTCA GATCTCGCCA
          121 CCATGGGTTC TCATCATCAT CATCATCATG GTATGGCTAG CATGACTGGT GGACAGCAAA
          181 TGGGTCGGGA TCTGTACGAC GATGACGATA AGGATCTCGC CACCATGGTC GACTCATCAC
          241 GTCGTAAGTG GAATAAGGCC GGTCACGCAG TCAGAGCTAT AGGTCGGCTG AGCTCACCCG
          301 TGGTGTCCGA GCGGATGTAC CCCGAGGACG GCGCCCTGAA GAGCGAGATC AAGAAGGGCC
          361 TGCGCCTGAA GGACGGCGGC CACTACGCCG CCGAGGTCAA GACCACCTAC AAGGCCAAGA
          421 AGCCCGTGCA GCTGCCCGGC GCCTACATCG TGGACATCAA GTTGGACATC GTGTCCCACA
          481 ACGAGGACTA CACCATCGTG GAACAGTGCG AACGCGCCGA GGGCCGCCAC TCCACCGGCG
          541 GCATGGACGA GCTGTACAAG GGCGGTACCG GAGGGAGCCT GGTGAGCAAG GGCGAGGAGG
          601 ACAACATGGC CATCATCAAG GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA
          661 ACGGCCACGA GTTCGAGATC GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GCCTTTCAGA
          721 CCGCTAAGCT TGTCGTCGTT AAGGGTGGTC CTCTGCCCTT CGCCTGGGAC ATCCTGTCCC
          781 CTCAGTTCAT GTACGGCTCC AAGGCCTACA TTAAGCACCC AGCCGACATC CCCGACTACT
          841 TCAAGCTGTC CTTCCCCGAG GGCTTCAGGT GGGAGCGCGT GATGAACTTC GAGGACGGCG
          901 GCATTATTCA CGTTAACCAG GACTCCTCCC TGCAAGACGG CGTGTTCATC TACAAGGTGA
          961 AGCTGCGCGG CACCAACTTC CCCCCCCGAC GGCCCCGTAA TGCAGAGAGA CCATGGCTGG
         1021 GAGGCCACGC GTGACCACTG ACTGAGAGCA GATCGCAGAT TAAGAGCCTT TCTCCTATTT
        1081 GACAGACGGC ATGGACATTA CCACAACGAA GCTGGGGGAC GGGTTAT
        //
        Primer: SV40polyA-R
        Sequence file: RDB14608_A7Bgb.seq check
        >D04021A3_A7Bg_1_SV40polyA-R_C11_08_ABI24.ab1
            1 GAGGAGGGGA GTTTTAAGCA AGTAAAACCT CTACAAATGT GGTATGGCTG ATTATGATCT
           61 AGAGTCGCGG CCGCTATGGG TTGGACTCCA CGTCTCCCGC CAACTTGAGA AGGTCAAAAT
          121 TCAAAGTCTG TTTCACCGGG AATTCACCAC CACCACCACC ACCACTGCCT CCAGTCCCTC
          181 CGCCCTTCGC TGTCATCATT TGTACAAACT CTTCGTAGTT TACCTGACCA TCCCCATCGA
          241 TGTCTGCCAC CCTGATCATT TCATCAACCT CTTCATCTGT TAACTTCTCT CCAAGGTCTG
          301 TCATCACGTG GCGAAGCTCT GCTGCGCCGA TGTAGCCATT GCCATCCTTA TCAAACACAC
          361 GGAACGCTTC TCTAATTTCT TCTTCACTGT CTGTGTCGTT CATTTTTCTT GCCATCATTG
          421 TCAGGAACTC AGGGAAGTCG AATGTGCCAT CACCGTCGGC ATCTACTTCA TTGATCATGT
          481 CCTGCAGCTC TGCTTCTGTG GGGTTCTGCC CCAGAGACCG CATCACCGTC CCCAGCTCCT
          541 TGGTTGTTAT TGTCCCATCC CCGTCCTTGT CAAATAGGGA GAAAGCCTCT TTAAATTCTG
          601 CGATCTGCTC TTCAGTCAGT TGGTCACGCG TGGCCTCCCA GCCCATGGTC TTCTTCTGCA
          661 TTACGGGGCC GTCGGGGGGG AAGTTGGTGC CGCGCAGCTT CACCTTGTAG ATGAACACGC
          721 CGTCTTGCAG GGAGGAGTCC TGGTTAACGT GAATAATGCC GCCGTCCTCG AAGTTCATCA
          781 CGCGCTCCCA CCTGAAGCCC TCGGGGAAGG ACAGCTTGAA GTAGTCGGGG ATGTCGGCTG
          841 GGTGCTTAAT GTAGGCCTTG GAGCCGTACA TGAACTGAGG GGACAGGATG TCCCAGGCGA
          901 AAGGGCAGAG GACCACCCTT AACGACGACA AGCTTAGCGG TCTGAAAGGC CTCGTAGGGG
          961 CGGCCCTCGC CCTCGCCCTC GATCTCGAAC TCGTGGCCGT TCACGGAGCC CTCCATGTGC
         1021 ACCTTGAGCG CATGACTCCT TGATGATGGC ATGTGTCTCT CGCCTGCTCA CAGCTCCTCG
         1081 TACGCCTGTA CAGCTCGTCA TGCCGCGTGA ATGCCGTCCT CGGCGCGGTT CGCCACTGTT
        1141 TTCCG
        //
        Primer: SV40pro_F_V2
        Sequence file: RDB14608_A7Bgc.seq check
        >D04021A3_A7Bg_1_SV40pro_F_V2_C12_09_ABI24.ab1
            1 AGCAGAGCTT TTTTTGGAAG CCTAGGCTTT TGCAAGATCG ATCAAGAGAC AGGATGAGGA
           61 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
          121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
          181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
          241 AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
          301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
          361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
          421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
          481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
          541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGAGC
          601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
          661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
          721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
          781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
          841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
          901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CTTCTATGAA AGGTTGGGCT
          961 TCGGAATCGT TTTCCCGGGA CGCCCGGCTG GATGATCCTC CCAGCGCGGG GGATCTCATG
         1021 CTGGAGTTCT TCGCCCCACC CCTAAGGGGG AGGCTACTGA ACACGGAAGA GACAATTACT
         1081 GATGACCGCG CTATGACGGC ATAAGACCGA TTAACGCACG ATGTGGGTCG TTTTGGTTTC
        1141 CATAAAC
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Ohkura, M., An improved genetically encoded red fluorescent Ca2+ indicator for detecting optically evoked action potentials. PLoS One 7 (7): e39933 (2012). PMID 22808076.
        reference Bethge, P., An R-CaMP1.07 reporter mouse for cell-type-specific expression of a sensitive red fluorescent calcium indicator. PLoS One 12 (6): e0179460 (2017). PMID 28640817.
        reference Inoue, M., Rational design of a high-affinity, fast, red calcium indicator R-CaMP2. Nat Methods 12 (1): 64-70 (2015). PMID 25419959.

        2022.05.17

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