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G-CaMP4.1

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP4.1.

Catalog number RDB14606
Resource name G-CaMP4.1
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP4 is a calcium sensor with a faster kinetics and a higher signal-to-noise ratio compared to G-CaMP2, G-CaMP4.1 is an improved form of G-CaMP4 that reduced side effects on intracellular signal transduction system.
Comment Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of insert sequence AKE14367.1, KP230535.1 (DDBJ accession number)
Depositor|Developer Nakai, Junichi | Ohkura, Masamichi |
Other clones in our bank

External Database
Rat Calm2 Chickin MLCK Aequorea victoria GFP

        Reference sequence
          

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Shindo, A. et al. PLos One 5 (2): e8897, 2010) and an acknowledgment to the DEPOSITOR are requested. The DEPOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
        Ordering Please visit Information of Request for Distribution.[link] 
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        Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
        This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
        提供案内 (日本国内) [open/close]

        提供条件 研究成果の公表にあたって寄託者の指定する文献(Shindo, A. et al. PLos One 5 (2): e8897, 2010)を引用し、謝辞の表明を必要とする。寄託者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に寄託者の求めに対して回答することとする。当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に寄託者との間で、別途使用許諾に係る契約の締結が必要である。
        提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
        提供依頼書 [Word]
        提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
        GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。

        Catalog # Resource name Availability Shipping form Fee (non-profit org.)
        RDB14606 G-CaMP4.1 Under QC test. Please contact us. DNA solution

        check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

        References and tips

        Electronic file

        Original reference

        original Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.

        Further references such as user reports and related articles (go to bottom)

        Featured content

        Featured content Dnaconda's recommendation EXR0067j (Japanese text)
        Featured content Calcium Ion Sensor Clones (English text)

        Sequence information


        References

        Original, user report and related articles

        original Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.

        2022.07.13

        GNP_filter3_RDBDEP_html_220713.pl