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G-CaMP4.1

Genetically-encoded fluorescent calcium sensor cDNA, G-CaMP4.1.

Catalog number RDB14606
Resource name G-CaMP4.1
Clone info. Genetically-encoded fluorescent calcium sensor cDNA. It encodes a fusion protein of EGFP, calmodulin, and the M13 peptide from myosin light chain kinase. G-CaMP4 is a calcium sensor with a faster kinetics and a higher signal-to-noise ratio compared to G-CaMP2, G-CaMP4.1 is an improved form of G-CaMP4 that reduced side effects on intracellular signal transduction system.
Expression was confirmed by the depositor with calcium imaging.
Vector backbone pEGFP-N1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^R (E. coli), Neo^r (mammalian cells).
Gene/insert name Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Reference of nucleotide sequence AKE14367.1, KP230535.1 (DDBJ accession number)
Depositor Nakai, Junichi | Ohkura, Masamichi |
 
Other clones in our bank Rat Calm2 (KEGG orthology K02183) | Chickin MYLK (KEGG orthology K00907) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the original publication designated by the DEPOSITOR (Shindo, A. et al. PLos One 5 (2): e8897, 2010) and an acknowledgment to the DEPOSITOR are requested. The DEPOOSITOR may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEPOSITOR. Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.
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Schedule A (GFP-A) [Link]
Remarks
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB14606 G-CaMP4.1 Under QC test. Please contact us. DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Original reference

original Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Dnaconda's recommendation EXR0067j (Japanese text)
Featured content Calcium Ion Sensor Clones (English text)

Sequence information


References

Original, user report and related articles

original Shindo, A., Tissue-tissue interaction-triggered calcium elevation is required for cell polarization during Xenopus gastrulation. PLoS One 5 (2): e8897 (2010). PMID 20126393.

2019.09.19

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