Please review the QC test results indicated by icon below as well as clone information before placing your order.

T7-3xFLAG-NLS-hCAS9-NLS-pA

Cas9-poly(A) expressing improved plasmid.

Sequence (full)	RDB14602zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) is requested. The RECIPIENT agrees to expressly describe the acknowledgment of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの使用は学術機関での学術研究に限る。利用者は、研究成果の公表にあたって寄託者の指定する文献 (Mizuno-Iijima, S., et al., Methods 191: 23-31 (2021)) を引用する。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14602_A7B9p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro,T7 pro,3XFLAG,SV40 NLS,insert 5' Sequence file: RDB14602_A7B9a.seq
>D04438A1_A7B9_1_CMV-Forward_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNGCNNAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA    61 TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT   121 TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG ACTCTAGTAA TACGACTCAC   181 TATAGGGAGA GCCGCCACCA TGGACTATAA GGACCACGAC GGAGACTACA AGGATCATGA   241 TATTGATTAC AAAGACGATG ACGATAAGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA   301 CGGAGTCCCA GCAGCCGACA AGAAGTACTC CATTGGGCTC GATATCGGCA CAAACAGCGT   361 CGGCTGGGCC GTCATTACGG ACGAGTACAA GGTGCCGAGC AAAAAATTCA AAGTTCTGGG   421 CAATACCGAT CGCCACAGCA TAAAGAAGAA CCTCATTGGC GCCCTCCTGT TCGACTCCGG   481 GGAGACGGCC GAAGCCACGC GGCTCAAAAG AACAGCACGG CGCAGATATA CCCGCAGAAA   541 GAATCGGATC TGCTACCTGC AGGAGATCTT TAGTAATGAG ATGGCTAAGG TGGATGACTC   601 TTTCTTCCAT AGGCTGGAGG AGTCCTTTTT GGTGGAGGAG GATAAAAAGC ACGAGCGCCA   661 CCCAATCTTT GGCAATATCG TGGACGANGT GGCGTACCAT GAAAAGTACC CNACCATATN   721 TNNTCTGANG AAGAANCTTG TANNCNGTAC TGATAAGGCT GACTTGCGGT TGATCTATCT   781 CGCGCTGGCG CATATGATCA AATTTCGGGG ANNCTTCCNC ATCGAGGGGG ANCTGAANCC   841 NGACANCAGC GATGNCGACA AACTCTTTAT CCNNCNGGTT NNNNNNTTNC NANCAGCNTT   901 TNCGANNANA NNCNGATCNA CGCNTNCNGN ANTTGNNGCC NANNNNNTCC TGANCGCTNN 961 NNNNTNNNAA NNCCNNNGNN NNNNNNNN //
Primer: HSV_TK_pA_R Region: SV40 NLS,insert 3' Sequence file: RDB14602_A7B9b.seq
>D04438A1_A7B9_1_HSV_TK_pA_R_G08_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCC TTCCGTGTTN NNNTTAGCCT CCNNNNNNTA    61 AACTCATTAC TAACCGGTAG GGATCGAACC CTTTCACACC TTCCTCTTCT TCTTGGGGTC   121 AGCCCTGCTG TCTCCACCGA GCTGAGAGAG GTCGATTCTT GTTTCATAGA GCCCCGTAAT   181 TGACTGATGA ATCAGTGTGG CGTCCAGGAC CTCCTTTGTA GAGGTGTACC GCTTTCTGTC   241 TATGGTGGTG TCGAAGTACT TGAAGGCTGC AGGCGCGCCC AAGTTGGTCA GAGTAAACAA   301 GTGGATAATG TTTTCTGCCT GCTCCCTGAT GGGCTTATCC CTGTGCTTAT TGTAAGCAGA   361 AAGCACCTTA TCGAGGTTAG CGTCGGCGAG GATCACTCTT TTGGAGAATT CGCTTATTTG   421 CTCGATGATC TCATCAAGGT AGTGTTTGTG TTGTTCCACG AACAGCTGCT TCTGCTCATT   481 ATCTTCGGGA GACCCTTTGA GCTTTTCNNA NTGGCTGGCC AGATNNNNNA AATTAACGTA   541 TTTAGAGGGC AGTGCCAGCT CGTTACCTTT CTGCAGCTCG CCCGCACTAG CNNNNNNTCG   601 TTTCCGGCCG TTTTCAAGCT CAAAGAGAGA GTACTNGNNA NGCTTANNGA TGAGGTCTTT   661 TTTGACCTCT TTATATCCTT TCNNCTCNAN NNNGTCGATG GGGTTTTTTT CGAAGCTTGA   721 TCNNNNNNNT GATTGTGATG NCCAGCNNTT CCTTGACGCT TTTGAGTTTT TTAGACTTCC   781 CTTTCTCCAC TTTGGCCACA ACCAGTACAC TGTAAAGCNN ANTGTANGAN AATCGANTCC   841 GCCGNATTNN TTGGGGGNNN CCNNNNCNTT TTTTGCGNGC NATCAGNTTN GTCGNNGNTN   901 NNNTTTTCNG NAGGANNNTT TCNNTNNGNA NNNNCCTCCG GGTCNGTACT TCNGNNNTTT 961 TTNNNGANNN TNNNCNTNGN NGGNNNNNGN NNAGGNACNN NNNNNNGNAN NNNNNNNNN //
Primer: hCAS9+2010/F Region: insert mid Sequence file: RDB14602_A7B9c.seq
>D04438A1_A7B9_1_hCAS9+2010_F_H08_23_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNGAT TTGNNNNNCG GAACTTCATG    61 CAGTTGATCC ATGATGACTC TCTCACCTTT AAGGAGGACA TCCAGAAAGC ACAAGTTTCT   121 GGCCAGGGGG ACAGTCTTCA CGAGCACATC GCTAATCTTG CAGGTAGCCC AGCTATCAAA   181 AAGGGAATAC TGCAGACCGT TAAGGTCGTG GATGAACTCG TCAAAGTAAT GGGAAGGCAT   241 AAGCCCGAGA ATATCGTTAT CGAGATGGCC CGAGAGAACC AAACTACCCA GAAGGGACAG   301 AAGAACAGTA GGGAAAGGAT GAAGAGGATT GAAGAGGGTA TAAAAGAACT GGGGTCCCAA   361 ATCCTTAAGG AACACCCAGT TGAAAACACC CAGCTTCAGA ATGAGAAGCT CTACCTGTAC   421 TACCTGCAGA ACGGCAGGGA CATGTACGTG GATCAGGAAC TGGACATCAA TCGGCTCTCC   481 GACTACGACG TGGATCATAT CGTGCCCCAG TCTTTTCTCA AAGATGATTC TATTGATAAT   541 AAAGTGTTGA CAAGATCCGA TAAAAATAGA GGGAAGAGTG ATAACGTCCC CTCAGAANAA   601 NTTGTCAAGA AAATGAAAAA TTATTGGCGG CAGCTGCTGA ACGCCAAACT GATCACACAA   661 CGGAAGTTCG ATAATCTGAC TAAGGCTGAA CGAGGTGGCC TGTCTGAGTT GGATAAAGCC   721 GGCTTCNTCA AAAGGCAGCT TGTTGAGACA CGCCAGATCA CCAAGCACGT GGCCCAAATT   781 CTCGATTCAC GCNTGAACAC CAAGNACGAT GAAAATGACA AACTGATTCG ANAGGTGAAA   841 GTTATTACTC TGAAGTCTAA GCTGGTCTCN NANNTCNGAA AANGANTTTC NNTTTTATNA   901 GGNGNNANAN ATNANCNNTN ANCNNNANNG CGCANGATGC CTACCTGGAN TGNAGNGGNN   961 NNGCNCTGNN NTNNTCAAAA AATATNCCNN NNNNTNGNAN NNGNNNTTTG NTTNNCNGNN  1021 NCTATNNNNN GNNCNNANNN TNGNNAAANN NATNNNNNAA GNNNNNNNNN GNNNNNNNNN 1081 N //
Primer: hCAS9+3550/R Region: insert mid Sequence file: RDB14602_A7B9d.seq
>D04438A1_A7B9_1_hCAS9+3550_R_A09_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGA NNGNGNNGCC CNNNNNNTCC TTGNCGCTTT    61 TGAGTTTTTT AGACTTCCCT TTCTCCACTT TGGCCACAAC CAGTACACTG TAAGCGACTG   121 TAGGAGAATC GAATCCGCCG TATTTCTTGG GGTCCCAATC TTTTTTGCGT GCGATCAGCT   181 TGTCGCTGTT CCTTTTCGGG AGGATACTTT CCTTGGAGAA GCCTCCGGTC TGTACTTCGG   241 TCTTTTTAAC GATGTTCACC TGCGGCATGG ACAGGACCTT CCGGACTGTC GCGAAATCCC   301 TACCCTTGTC CCACACGATT TCTCCTGTTT CTCCGTTTGT TTCGATAAGT GGTCGCTTCC   361 GAATCTCTCC ATTGGCCAGT GTAATCTCGG TCTTGAAAAA ATTCATAATA TTGCTGTAAA   421 AGAAGTACTT AGCGGTGGCC TTGCCTATTT CCTGCTCAGA CTTTGCGATC ATTTTCCTAA   481 CATCGTACAC TTTATAGTCT CCGTAAACAA ATTCAGATTC AAGCTTGGGA TATTTTTTGA   541 TAAGTGCAGT GCCTACCACT GCATTCAGGT AGGCATCATG CGCATGGTGG TAATTGTTGA   601 TCTCTCTCAC CTTATAAAAC TGAAAGTCCT TTCTGAAATC TGAGACCAGC TTAGACTTCA   661 GAGTAATAAC TTTCACCTCT CGAATCAGTT TGTCATTTTC ATCGTACTTG GTGTTCATGC   721 GTGAATCGAG AATTTGGGCC ACGTGCTTGG TGATCTGGCG TGTCTCAACA AGCTGCCTTT   781 TGANGANNNN GGCTTTATCN NACTCANACA NGGCCACCTC GTTCANCCTT ANNCAGANTA   841 TCGAACTTCC GTTGTGTGAT CAGTTNNGNN GTTCANCANC TGNCGCNNAT ANTTTTTCAT   901 TTTNCTNGAC ANNTTCTTNT GANGGGANGT TNNNACNCTN NCCTCTATTT TNATCGGANN   961 TTGNCNNNCT TTNNNATCNN NANANNCATC TTNNNNNNAN NNNNGGGGGN NNNANNTGAT 1021 CCNNNNNNNA NNNNNANANN CNANNNNNNG NNNNNNNNNN //
Primer: f1ori_F Region: f1 ori,p A Sequence file: RDB14602_A7B9e.seq
>D04438A1_A7B9_1_f1ori_F_B09_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGCGNNNNN NCTGCGCGTA ACCACCACAC    61 CCGCCGCGCT TAATGCGCCG CTACAGGGCG CGTGGGGATA CCCCCTAGAG CCCCAGCTGC   121 GCAGATCTGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC   181 CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG   241 GTCTCGGTGG GGTATCGATG TACAGCTAGC TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 301 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTNN NNNNNNNN //
Primer: SV40pro_F Region: NeoR Sequence file: RDB14602_A7B9f.seq
>D04438A1_A7B9_1_SV40pro_F_C09_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGNGGANGN TTTTTTGGAG GNCTAGGCTT    61 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCANN ANANNGGATG   121 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT   181 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT   241 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC   301 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC   361 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA   421 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT   481 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA   541 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA   601 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC   661 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT   721 CATGGTGGAA AATGNCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA   781 NCGCTATCAG GACATAGCGT TGGCTACNCG TGATATTGCT GAAGANCTTG NCGGCGAATG   841 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCANC GCATCGCCTT   901 CTATCGCCNT NCTTGACNAN NNCTTCTGAN CGNNACTCTG GGGTTCGCGA AATGANCGAN 961 NNAGCGACNN CCNANCNNNN NNTCNCNANN NTTCNATTNN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl