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pCMV-HA-Bach2 L687A

Expression vector of HA-tagged mouse Bach2, L687A mutant.

Catalog number RDB14570
Resource name pCMV-HA-Bach2 L687A
Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L687A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.
Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor Yamashita, Masakatsu |
 
Other clones in our bank mouse Bach2 (NCBI Gene 12014) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
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RDB14570 pCMV-HA-Bach2 L687A DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14570_A7B6p1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14570_A7B6a.seq
>D04207A2_A7B6_1_CMV Forward_C12_03_ABI08.ab1
    1 ACGTCCACTT CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG
  121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGAGGAT CCGGTACTAG
  181 AGGAACTGAA AAACCAGAAA GTTAACTGGT AAGTTTAGTC TTTTTGTCTT TTATTTCAGG
  241 TCCCGGATCC GGTGGTGGTG CAAATCAAAG AACTGCTCCT CAGTGGATGT TGCCTTTACT
  301 TCTAGGCCTG TACGGAAGTG TTACTTCTGC TCTAAAAGCT GCGGAATTGT ACCCGCGGGC
  361 CCACCATGTA CCCATACGAT GTTCCAGATT ACGCTCTTAT GGCCATGGAG GCCCGAATTC
  421 GGTCGACCAT GTCTGTGGAT GAGAAGCCTG GCTCCCCCAT GTATGTATAT GAGTCCACAG
  481 TCCACTGTGC CAACATCCTC CTGGGCCTCA ATGACCAACG GAAGAAGGAC ATTCTCTGTG
  541 ACGTGACGCT GATCGTGGAG AGGAAGGAGT TCCGAGCCCA CCGGGCTGTG CTGGCCGCAT
  601 GCAGTGAATA CTTCTGGCAA GCACTGGTTG GACAGACGAA AGATGACTTG GTGGTCAGCT
  661 TGCCTGAGGA GGTCACGGCC AGGGGCTTTG GGCCACTGCT ACAGTTTGCC TACACTGCCA
  721 AGCTGTTACT CAGCAGAGAG AACATCCGGG AGGTCATCCG CTGTGCTGAG TTCCTGCGCA
  781 TGCACAATCT GGAGGACTCC TGCTTCAGCT TCCTGCAGAC CCAGCTCCTG AACAGGGAGG
  841 ATGGCCTGTT TGTTTGCAGA AAGGACAGTG CGTGCCAGCG CCCCCAAGAG GACCATGGGA
  901 ATTCTGCAGG AGAAGAGGAG GAAGAAAGAG GAAACAATGG ACTCAGAGAC AGCTAGGATG
  961 GCTTGCGCCA CAGACCAGAT GCTCCCAGGA CCCCCATCAG CTTTGAGGCA CTGCCATCCC
 1021 AGTAGCAGAG GAAGAGAGCC CTGCTCCCTG AGTCTGAGTA ACCCACAGAA CACCAGAACT
1081 CAGAAAGGTT GCATGGACCC AGTAACCCTT AGAATTATTT AAG
//
Primer: pAxCALNLF1 RDB14570_A7B6b.seq
>D04207A2_A7B6_1_pAxCALNLF1_D12_04_ABI08.ab1
    1 TCCATTTATC TTATCATGTC TGGATCCCCG CGGCCGCCTA GGCATAATCT TTCCTGGGCT
   61 GTTCGTCCGT TGTACACTTC TCAGTCATTT CCTGGCAGAA GTCCACCGTC ACAGACTGGC
  121 TCCTGGCTTC TAGGGGAGGT CCTCGTTCTG AGAATGTTCC AGGGTCACTG CCTTCCAACC
  181 TCCTCCCAGA GGTACAATTC TCAGAGGTGT TGCTGGGCAC CCAGGGGAGC CCGGGGGGAG
  241 CCGCCCCTGG CTCCAGGCAG CAGGGTACAC TTCCTCCTAC TGCACACGGG GAGGGTGCAA
  301 GGCTCTGCTC AACACCCACA GGAGGAGGGT TAACACTGGC TCCAGGGAGA TCCATGGGTA
  361 TGAGGACAGG GCAGTAGCGG TGCAGGGCCT GGATCTGCTC TGGACTCTGG ATGTCTCGAC
  421 AGACCTCCTG GGAGAGGCAG GAAAAGTTGT CCAGGAGCTC TCCCATGCAC GCCTTCAGGT
  481 GATTCCTCTC CGACAATGCT TTTTCCTTCT CGCACACCAG TTTCCGTATT TCACATTCCA
  541 AGTTCTGAAT ACAGTCCAGC TTCCTCTTTC GACAGCGCTG GGCTGCAATG CGGTTCTTAC
  601 TCCGCCTCCG GATGTCGTGG ATGAACTCCA ACTGTTCTGA GGTTAGCTTG TGCATTTTGA
  661 TCATCATCTG GAAGTCGTTC CTGGGAAGGT CTGTGATCTG ATCGACAGGA AAAGGAAGTT
  721 TAACCTCCTG GCCCCTGTCC TGCACAGGAC ACGACTCACT GTCTGCTTCC GAGAATGATC
  781 CAGACTCGTC ACTGGAATTG GTTCCGTAAG ACTGCTCACA TTTAATCTGG GGTCGGACTT
  841 GGTTGTACAT TCCATCTCCC ATCACGCCTG GTTCCTGATG TTCCGTGGCA AGGAATCTGG
  901 CTCCCTGGGA ACAGGGCGAA GAGGAGAACT CACAGAGAGG GAGGCTGCAG GGGGAGCCCC
  961 CACTCCCGTT CCTCTGCGTA GGAAATACGA GGAGCAGGAG CTGGAGTCCT AGTCCCTGAT
 1021 CTCGAGCGGA GTGAGCGAGG ACAGGACCTT GATGCAACCG GCAGCTGTGA TGGCGTATCG
1081 TTCTGACAAG TGGATCAGGC CCAATTCCCA G
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

2018.12.11

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