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pCMV-HA-Bach2 L687A (#RDB14570)

Expression vector of HA-tagged mouse Bach2, L687A mutant.

Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L687A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor|Developer Yamashita, Masakatsu |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14570 pCMV-HA-Bach2 L687A DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCMV-HA-Bach2 L687A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14570).

Reference section:

Kuwahara, M., Ise, W., Ochi, M., Suzuki, J., Kometani, K., Maruyama, S., Izumoto, M., Matsumoto, A., Takemori, N., Takemori, A., Shinoda, K., Nakayama, T., Ohara, O., Yasukawa, M., Sawasaki, T., Kurosaki, T., Yamashita, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14570_A7B6p1_2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB14570_A7B6a.seq check
>D04207A2_A7B6_1_CMV Forward_C12_03_ABI08.ab1
    1 ACGTCCACTT CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
   61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG
  121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGAGGAT CCGGTACTAG
  181 AGGAACTGAA AAACCAGAAA GTTAACTGGT AAGTTTAGTC TTTTTGTCTT TTATTTCAGG
  241 TCCCGGATCC GGTGGTGGTG CAAATCAAAG AACTGCTCCT CAGTGGATGT TGCCTTTACT
  301 TCTAGGCCTG TACGGAAGTG TTACTTCTGC TCTAAAAGCT GCGGAATTGT ACCCGCGGGC
  361 CCACCATGTA CCCATACGAT GTTCCAGATT ACGCTCTTAT GGCCATGGAG GCCCGAATTC
  421 GGTCGACCAT GTCTGTGGAT GAGAAGCCTG GCTCCCCCAT GTATGTATAT GAGTCCACAG
  481 TCCACTGTGC CAACATCCTC CTGGGCCTCA ATGACCAACG GAAGAAGGAC ATTCTCTGTG
  541 ACGTGACGCT GATCGTGGAG AGGAAGGAGT TCCGAGCCCA CCGGGCTGTG CTGGCCGCAT
  601 GCAGTGAATA CTTCTGGCAA GCACTGGTTG GACAGACGAA AGATGACTTG GTGGTCAGCT
  661 TGCCTGAGGA GGTCACGGCC AGGGGCTTTG GGCCACTGCT ACAGTTTGCC TACACTGCCA
  721 AGCTGTTACT CAGCAGAGAG AACATCCGGG AGGTCATCCG CTGTGCTGAG TTCCTGCGCA
  781 TGCACAATCT GGAGGACTCC TGCTTCAGCT TCCTGCAGAC CCAGCTCCTG AACAGGGAGG
  841 ATGGCCTGTT TGTTTGCAGA AAGGACAGTG CGTGCCAGCG CCCCCAAGAG GACCATGGGA
  901 ATTCTGCAGG AGAAGAGGAG GAAGAAAGAG GAAACAATGG ACTCAGAGAC AGCTAGGATG
  961 GCTTGCGCCA CAGACCAGAT GCTCCCAGGA CCCCCATCAG CTTTGAGGCA CTGCCATCCC
 1021 AGTAGCAGAG GAAGAGAGCC CTGCTCCCTG AGTCTGAGTA ACCCACAGAA CACCAGAACT
1081 CAGAAAGGTT GCATGGACCC AGTAACCCTT AGAATTATTT AAG
//
Primer: pAxCALNLF1
Sequence file: RDB14570_A7B6b.seq check
>D04207A2_A7B6_1_pAxCALNLF1_D12_04_ABI08.ab1
    1 TCCATTTATC TTATCATGTC TGGATCCCCG CGGCCGCCTA GGCATAATCT TTCCTGGGCT
   61 GTTCGTCCGT TGTACACTTC TCAGTCATTT CCTGGCAGAA GTCCACCGTC ACAGACTGGC
  121 TCCTGGCTTC TAGGGGAGGT CCTCGTTCTG AGAATGTTCC AGGGTCACTG CCTTCCAACC
  181 TCCTCCCAGA GGTACAATTC TCAGAGGTGT TGCTGGGCAC CCAGGGGAGC CCGGGGGGAG
  241 CCGCCCCTGG CTCCAGGCAG CAGGGTACAC TTCCTCCTAC TGCACACGGG GAGGGTGCAA
  301 GGCTCTGCTC AACACCCACA GGAGGAGGGT TAACACTGGC TCCAGGGAGA TCCATGGGTA
  361 TGAGGACAGG GCAGTAGCGG TGCAGGGCCT GGATCTGCTC TGGACTCTGG ATGTCTCGAC
  421 AGACCTCCTG GGAGAGGCAG GAAAAGTTGT CCAGGAGCTC TCCCATGCAC GCCTTCAGGT
  481 GATTCCTCTC CGACAATGCT TTTTCCTTCT CGCACACCAG TTTCCGTATT TCACATTCCA
  541 AGTTCTGAAT ACAGTCCAGC TTCCTCTTTC GACAGCGCTG GGCTGCAATG CGGTTCTTAC
  601 TCCGCCTCCG GATGTCGTGG ATGAACTCCA ACTGTTCTGA GGTTAGCTTG TGCATTTTGA
  661 TCATCATCTG GAAGTCGTTC CTGGGAAGGT CTGTGATCTG ATCGACAGGA AAAGGAAGTT
  721 TAACCTCCTG GCCCCTGTCC TGCACAGGAC ACGACTCACT GTCTGCTTCC GAGAATGATC
  781 CAGACTCGTC ACTGGAATTG GTTCCGTAAG ACTGCTCACA TTTAATCTGG GGTCGGACTT
  841 GGTTGTACAT TCCATCTCCC ATCACGCCTG GTTCCTGATG TTCCGTGGCA AGGAATCTGG
  901 CTCCCTGGGA ACAGGGCGAA GAGGAGAACT CACAGAGAGG GAGGCTGCAG GGGGAGCCCC
  961 CACTCCCGTT CCTCTGCGTA GGAAATACGA GGAGCAGGAG CTGGAGTCCT AGTCCCTGAT
 1021 CTCGAGCGGA GTGAGCGAGG ACAGGACCTT GATGCAACCG GCAGCTGTGA TGGCGTATCG
1081 TTCTGACAAG TGGATCAGGC CCAATTCCCA G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382. [link to RRC of NBRP]