Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMV-HA-Bach2 L687A

Expression vector of HA-tagged mouse Bach2, L687A mutant.

Catalog number RDB14570
Resource name pCMV-HA-Bach2 L687A
Clone info. Expression vector of HA-tagged mouse Bach2 point mutation into the leucine zipper region, L687A, which abrogates interaction with Batf. Refer Fig. 5 of Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016.
Comment Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor|Developer Yamashita, Masakatsu |
Other clones in our bank
External Database
Mouse Bach2
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14570 pCMV-HA-Bach2 L687A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14570_A7B6p1_2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14570_A7B6a.seq check
          >D04207A2_A7B6_1_CMV Forward_C12_03_ABI08.ab1
              1 ACGTCCACTT CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG
            121 ACCTCCATAG AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGAGGAT CCGGTACTAG
            181 AGGAACTGAA AAACCAGAAA GTTAACTGGT AAGTTTAGTC TTTTTGTCTT TTATTTCAGG
            241 TCCCGGATCC GGTGGTGGTG CAAATCAAAG AACTGCTCCT CAGTGGATGT TGCCTTTACT
            301 TCTAGGCCTG TACGGAAGTG TTACTTCTGC TCTAAAAGCT GCGGAATTGT ACCCGCGGGC
            361 CCACCATGTA CCCATACGAT GTTCCAGATT ACGCTCTTAT GGCCATGGAG GCCCGAATTC
            421 GGTCGACCAT GTCTGTGGAT GAGAAGCCTG GCTCCCCCAT GTATGTATAT GAGTCCACAG
            481 TCCACTGTGC CAACATCCTC CTGGGCCTCA ATGACCAACG GAAGAAGGAC ATTCTCTGTG
            541 ACGTGACGCT GATCGTGGAG AGGAAGGAGT TCCGAGCCCA CCGGGCTGTG CTGGCCGCAT
            601 GCAGTGAATA CTTCTGGCAA GCACTGGTTG GACAGACGAA AGATGACTTG GTGGTCAGCT
            661 TGCCTGAGGA GGTCACGGCC AGGGGCTTTG GGCCACTGCT ACAGTTTGCC TACACTGCCA
            721 AGCTGTTACT CAGCAGAGAG AACATCCGGG AGGTCATCCG CTGTGCTGAG TTCCTGCGCA
            781 TGCACAATCT GGAGGACTCC TGCTTCAGCT TCCTGCAGAC CCAGCTCCTG AACAGGGAGG
            841 ATGGCCTGTT TGTTTGCAGA AAGGACAGTG CGTGCCAGCG CCCCCAAGAG GACCATGGGA
            901 ATTCTGCAGG AGAAGAGGAG GAAGAAAGAG GAAACAATGG ACTCAGAGAC AGCTAGGATG
            961 GCTTGCGCCA CAGACCAGAT GCTCCCAGGA CCCCCATCAG CTTTGAGGCA CTGCCATCCC
           1021 AGTAGCAGAG GAAGAGAGCC CTGCTCCCTG AGTCTGAGTA ACCCACAGAA CACCAGAACT
          1081 CAGAAAGGTT GCATGGACCC AGTAACCCTT AGAATTATTT AAG
          //
          Primer: pAxCALNLF1
          Sequence file: RDB14570_A7B6b.seq check
          >D04207A2_A7B6_1_pAxCALNLF1_D12_04_ABI08.ab1
              1 TCCATTTATC TTATCATGTC TGGATCCCCG CGGCCGCCTA GGCATAATCT TTCCTGGGCT
             61 GTTCGTCCGT TGTACACTTC TCAGTCATTT CCTGGCAGAA GTCCACCGTC ACAGACTGGC
            121 TCCTGGCTTC TAGGGGAGGT CCTCGTTCTG AGAATGTTCC AGGGTCACTG CCTTCCAACC
            181 TCCTCCCAGA GGTACAATTC TCAGAGGTGT TGCTGGGCAC CCAGGGGAGC CCGGGGGGAG
            241 CCGCCCCTGG CTCCAGGCAG CAGGGTACAC TTCCTCCTAC TGCACACGGG GAGGGTGCAA
            301 GGCTCTGCTC AACACCCACA GGAGGAGGGT TAACACTGGC TCCAGGGAGA TCCATGGGTA
            361 TGAGGACAGG GCAGTAGCGG TGCAGGGCCT GGATCTGCTC TGGACTCTGG ATGTCTCGAC
            421 AGACCTCCTG GGAGAGGCAG GAAAAGTTGT CCAGGAGCTC TCCCATGCAC GCCTTCAGGT
            481 GATTCCTCTC CGACAATGCT TTTTCCTTCT CGCACACCAG TTTCCGTATT TCACATTCCA
            541 AGTTCTGAAT ACAGTCCAGC TTCCTCTTTC GACAGCGCTG GGCTGCAATG CGGTTCTTAC
            601 TCCGCCTCCG GATGTCGTGG ATGAACTCCA ACTGTTCTGA GGTTAGCTTG TGCATTTTGA
            661 TCATCATCTG GAAGTCGTTC CTGGGAAGGT CTGTGATCTG ATCGACAGGA AAAGGAAGTT
            721 TAACCTCCTG GCCCCTGTCC TGCACAGGAC ACGACTCACT GTCTGCTTCC GAGAATGATC
            781 CAGACTCGTC ACTGGAATTG GTTCCGTAAG ACTGCTCACA TTTAATCTGG GGTCGGACTT
            841 GGTTGTACAT TCCATCTCCC ATCACGCCTG GTTCCTGATG TTCCGTGGCA AGGAATCTGG
            901 CTCCCTGGGA ACAGGGCGAA GAGGAGAACT CACAGAGAGG GAGGCTGCAG GGGGAGCCCC
            961 CACTCCCGTT CCTCTGCGTA GGAAATACGA GGAGCAGGAG CTGGAGTCCT AGTCCCTGAT
           1021 CTCGAGCGGA GTGAGCGAGG ACAGGACCTT GATGCAACCG GCAGCTGTGA TGGCGTATCG
          1081 TTCTGACAAG TGGATCAGGC CCAATTCCCA G
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl