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pCMV-HA-Bach2 wt

Expression vector of HA-tagged mouse Bach2, wild type.

Catalog number RDB14569
Resource name pCMV-HA-Bach2 wt
Clone info. Expression vector of HA-tagged mouse Bach2, wild type.
Expression was confirmed by the depositor with immunoprecipitation and immunoblot using anti HA antibody, respectively.
Vector backbone pCMV-HA (plasmid)
Size of vector backbone 3.8 kb
Selectable markers Amp^r
Gene/insert name Mouse Bach2 cDNA
Depositor Yamashita, Masakatsu |
 
Other clones in our bank mouse Bach2 (NCBI Gene 12014) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kuwahara, M. et al. Nat. Commun. 7: 12596, 2016)
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RDB14569 pCMV-HA-Bach2 wt DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14569_A7B6p1_2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB14569_A7B6a.seq
>D04207A1_A7B6_1_CMV Forward_A12_01_ABI08.ab1
    1 ACGTTCAACT CCGGCCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT
  121 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG GACTCTAGAG GATCCGGTAC
  181 TAGAGGAACT GAAAAACCAG AAAGTTAACT GGTAAGTTTA GTCTTTTTGT CTTTTATTTC
  241 AGGTCCCGGA TCCGGTGGTG GTGCAAATCA AAGAACTGCT CCTCAGTGGA TGTTGCCTTT
  301 ACTTCTAGGC CTGTACGGAA GTGTTACTTC TGCTCTAAAA GCTGCGGAAT TGTACCCGCG
  361 GGCCCACCAT GTACCCATAC GATGTTCCAG ATTACGCTCT TATGGCCATG GAGGCCCGAA
  421 TTCGGTCGAC CATGTCTGTG GATGAGAAGC CTGGCTCCCC CATGTATGTA TATGAGTCCA
  481 CAGTCCACTG TGCCAACATC CTCCTGGGCC TCAATGACCA ACGGAAGAAG GACATTCTCT
  541 GTGACGTGAC GCTGATCGTG GAGAGGAAGG AGTTCCGAGC CCACCGGGCT GTGCTGGCCG
  601 CATGCAGTGA ATACTTCTGG CAAGCACTGG TTGGACAGAC GAAAGATGAC TTGGTGGTCA
  661 GCTTGCCTGA GGAGGTCACG GCCAGGGGCT TTGGGCCACT GCTACAGTTT GCCTACACTG
  721 CCAAGCTGTT ACTCAGCAGA GAGAACATCC GGGAGGTCAT CCGCTGTGCT GAGTTCCTGC
  781 GCATGCACAA TCTGGAGGAC TCCTGCTTCA GCTTCCTGCA GACCCAGCTC CTGAACAGGG
  841 AGGATGGCCT GTTTGTTTGC AGAAAGGACA GTGCGTGCCA GCGCCCCCAA GAGGGACCAT
  901 GGGGAATTCT GCAGGGAGAA GAGGAGGAAG AAGAGGAAAC AATGGACTCA GAGACAGCTA
  961 GGGATGGCTT GCGCCACAGA CCAGATGCTC CCAGACCCCA TCAGCTTTGA GGCCACTGCC
 1021 ATCCCAGTAG CAGAGAAAGA GAAGTCCTGC TCCCTGAGTC TTGAGTACCC ACAGAACACC
1081 AGAACTCGAA GGTGCATTGA CCAGTACCCT AGGAATATTA
//
Primer: pAxCALNLF1 RDB14569_A7B6b.seq
>D04207A1_A7B6_1_pAxCALNLF1_B12_02_ABI08.ab1
    1 CACCAGGTTA TCTTTATCAT GTCTGGATCC CCGCGGCCGC CTAGGCATAA TCTTTCCTGG
   61 GCTGTTCGTC CGTTGTACAC TTCTCAGTCA TTTCCTGGCA GAAGTCCACC GTCACAGACT
  121 GGCTCCTGGC TTCTAGGGGA GGTCCTCGTT CTGAGAATGT TCCAGGGTCA CTGCCTTCCA
  181 ACCTCCTCCC AGAGGTACAA TTCTCAGAGG TGTTGCTGGG CACCCAGGGG AGCCCGGGGG
  241 GAGCCGCCCC TGGCTCCAGG CAGCAGGGTA CACTTCCTCC TACTGCACAC GGGGAGGGTG
  301 CAAGGCTCTG CTCAACACCC ACAGGAGGAG GGTTAACACT GGCTCCAGGG AGATCCATGG
  361 GTATGAGGAC AGGGCAGTAG CGGTGCAGGG CCTGGATCTG CTCTGGACTC TGGATGTCTC
  421 GACAGACCTC CTGGGAGAGG CAGGAAAAGT TGTCCAGGAG CTCTCCCATG CACGCCTTCA
  481 GGTGATTCCT CTCCGACAAC AGTTTTTCCT TCTCGCACAC CAGTTTCCGT ATTTCACATT
  541 CCAAGTTCTG AATACAGTCC AGCTTCCTCT TTCGACAGCG CTGGGCTGCA ATGCGGTTCT
  601 TACTCCGCCT CCGGATGTCG TGGATGAACT CCAACTGTTC TGAGGTTAGC TTGTGCATTT
  661 TGATCATCAT CTGGAAGTCG TTCCTGGGAA GGTCTGTGAT CTGATCGACA GGAAAAGGAA
  721 GTTTAACCTC CTGGCCCCTG TCCTGCACAG GACACGACTC ACTGTCTGCT TCCGAGAATG
  781 ATCCAGACTC GTCACTGGAA TTGGTTCCGT AAGACTGCTC ACATTTAATC TGGGGTCGGA
  841 CTTGGTTGTA CATTCCATCT CCCATCACGC CTGGTTCCTG ATGTTCCGTG GCAAGGGAAT
  901 CTGGCTCCCT GGGAACAGGG CGAAGAGGAG AACTCACAGA GAGGAGGCTG CAGGGGGGGA
  961 GCCCCCCACT CCCGTCCTCT GCGTAGGAAT ACGAGGAGCA AGAGCTGGAG GTCTAGTCCT
 1021 GGTCTCGAGC GAGGTGAGCG GAGACAGACC TTGGATGGCA ACGGCAGCTT GTGATGGCCG
1081 TAATCCTACT TGCAGTTGAT CAGCCCATCC GAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Kuwahara, M., Bach2-Batf interactions control Th2-type immune response by regulating the IL-4 amplification loop. Nat. Commun. 7: 12596 (2016). PMID 27581382.

2018.12.11

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