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pactb-loxP-mCherry-loxP-EGFP

Expression vector of loxP-mCherry-loxP-EGFP under the medaka (Oryzias latipes) beta-actin promoter.

Catalog number RDB14532
Resource name pactb-loxP-mCherry-loxP-EGFP
Clone info. Expression vector of loxP-mCherry-loxP-EGFP under the medaka (Oryzias latipes) beta-actin promoter for the genome engineering. The structure of the plasmid construct, see Fig. 1 of Kishimoto, K. et al., Dev. Growth Differ., 58 (6): 516-521, 2016.
Comment Please refer AB557594 for the parental plasmid beta-act-loxP-RFP-loxP-GFP.
Vector backbone pEGFP-1 (Plasmid)
Size of vector backbone 4.1 kb
Selectable markers Amp^r
Gene/insert name Oryzias latipes actb genomic DNA
Depositor|Developer Kinoshita, Masato |
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External Database
Oryzias latipes actb

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)
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          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. (TBUSA). Please refer to Clontech Limited Use Label License and use Exclusive MTA (download the LULL and the MTA [link]).
          This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
          Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
          This RESOURCE contains a fluorescent protein owned or licensed by Takara Bio USA, Inc. A part of license fees is required. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。 Clontech Limited Use Label Licenseをご一読の上専用MTAの提出をお願いします (専用MTA (英語版のみ) とClontech Limited Use Label Licenseを入手[link])。
          GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
          備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。
          Takara Bio USA, Inc.が保有している蛍光タンパク質を含みます。ライセンス料相当額を負担して頂きます。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14532 pactb-loxP-mCherry-loxP-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14532_A7Adp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: OI bapro_F
          Region: OI beta-actin promoter 3', mCherry 5'
          Sequence file: RDB14532_A7Ada.seq check
          >D04061A1_A7Ad_1_OI bacpro_F_E06_15_ABI24.ab1
              1 CGCAGCAATG GGCTCTGACT GCAAGCAGAT GCAGATGGGC GTGTCTTTGG TGTTAACGCC
             61 TTTTTTGTTT GTTCTTTTCC AGTTTAGCCG TCGACATAAC TTCGTATAGC ATACATTATA
            121 CGAAGTTATA CTAGTACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG
            181 GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC
            241 GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC
            301 AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC
            361 AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG
            421 GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG
            481 GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC
            541 CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG
            601 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
            661 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
            721 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
            781 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
            841 TACAAGTAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA
            901 CTTGCTTTAA AAAAACCTCC CACACCTCCC CCCTGAACCT GAAACATAAA ATGAATGCAA
            961 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
           1021 CAAATTTCAC AAATAAAGCA TTTTTTTTTT TCACTGCATT TCTAGTTGTG GGATTTGTCC
           1081 CAAACCTTCA ATCCAAATTG CTATTCTTTA AGACGTAAAT GGTAGCGTAT TAACATATGA
          1141 ATATTCCG
          //
          Primer: mCherry-C_F
          Region: mCherry 3', EGFP 5'
          Sequence file: RDB14532_A7Adb.seq check
          >D04061A1 A7Ad-1-mcherry-CF_A03_03_ABI24.ab1
              1 CAGTAGGTCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC
             61 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC
            121 GGCGGCATGG ACGAGCTGTA CAAGTAGCGG CCGCGACTCT AGATCATAAT CAGCCATACC
            181 ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA
            241 CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
            301 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
            361 GGTTTGTCCA AACTCATCAA TGTATCTTAA GGCGTAAATT GTAAGCGTTA ATTAACATAT
            421 GATATCGATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCGCGGGCC CGGGATCCAC
            481 CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
            541 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
            601 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC
            661 CCTGCCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CCGCTACCCC
            721 GACCACATGA AGCAGCACGA ACTCTCAGTT CGGCATGGCC CCGTGCTACG TCCAGGAGCG
          781 CCACCATTCT TTTCCTTTCC AAGGGGAA
          //
          Primer: EGFP-N_F
          Region: EGFP 3'
          Sequence file: RDB14532_A7Adc.seq check
          >D04061A1 A7Ad-1-EGFP-NF_B03_06_ABI24.ab1
              1 TAGCGAGTCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
             61 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC
            121 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
            181 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
            241 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
            301 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC
            361 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC
            421 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC
            481 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG
            541 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC
            601 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGCGACTC TAGATCATAA
            661 TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC
            721 TGAACCTGAA ACATAAAATG ATTGCAGTGT GTGGTAACTG CTTTATTGCA GCTTTATAAT
          781 GGTTTACCAA AATTA
          //
          Primer: SV40pA3'element Reverse
          Region: ColE1 ori, OI beta-actin promoter 5'
          Sequence file: RDB14532_A7Add.seq check
          >D04061A1_A7Ad_1_SV40pA3'elementReverse_E02_05_ABI08.ab1
              1 GAGGCAGGGG GGGCGGAGCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
             61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
            121 ACCGTATTAC CGCCATGCAT TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA
            181 AGCTTCGAAT TCAATTACAG TGGTCATGAA TATTATATCT AACCACAGAG CTAAAGAAAA
            241 AACATACCAA GACAGTTTTT TTTCTGTTGA TGGGTGGTCA TTCTACATTG AAGTGAATGG
            301 GGTCTGACTG GTTTGTTTTA CGTGTCAGCT AGTGCTTTCT TGGTGAACTG ATCTGGCTCA
            361 TTTTTTTACT CTGACCTCAT CTGAGAATTG TTCAAAACAA ATGCACGATA TTTAATACTG
            421 CTGATAAGAA GGATTCGCTT CACTTGCAGG TGAACCCTGG TGTGGCAATA GTGTTAATGA
            481 AAACAAACTA CTCGGCAGTA AAAGTATGTG ACATGAATAA AAAAAAATCA CAAACTGGAA
            541 GAAGATCAGA TCATGAAAGA ATTGTAAGAA TGTCCCATTG TACGATGTGT TGTGGTAAAG
            601 TGGTGAAGTC ACTTATCAAT CCATGATGGT CAGAGAACTA GCAGGACTGG TTTTCTTTTC
            661 TTTGTTTTTT TTTATTTCCT TCATCCTGGT ACTGCCCACT TCTGGCCTTC AAACAAAGTC
            721 AAGTATTTTT TTTATTCAAA AGCAGATCAA ATTTAGAAGG TAACATCATC TGCCCATTTG
            781 AATCAAGTCT GTAGAACTAT CTTAGTATGA ACTTGCCATA ATGCTTCATA AGAAGTGATT
            841 GCAAATCACC TACCACACAG CTGGATGAGC TGGAACTCTG ACACATACAT TCATTCACCT
            901 GCAATCACGT AAAGGAGCCT ACTACGAAGG GACACTCCTG TGAGGCAGGA TTACATCCTT
            961 TTCCCAGCCC AACCCCCTGT CTGTGTTTTT CCCAGTTTCA GCAGTCAGTC CTTTCCAGTG
           1021 TGCTCTTACT TTACCAAGAA CCCGGCCCCT CCAGATACTG ACAATAGGAA GTACCATGTG
          1081 TTTTTAGAGT GAACGCCCAC CCTTGGAATG GGGAT
          //
          Primer: SV40pro_F_V2
          Region: SV40 promoter/ori, NeoR/KanR
          Sequence file: RDB14532_A7Ade.seq check
          >D04061A1 A7Ad-1-SV40proFv2_C03_09_ABI24.ab1
              1 AACTTTTTTG GAAGCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
             61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
            121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
            181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
            241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
            301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
            361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
            421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
            481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
            541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
            601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
            661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            721 GACATAGCGT TGGGCTACCG TTGATATGCT GAGAGCCTTG GTCGGCCGAA TGGGGCTGAC
          781 CGCCTTCGTT CG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl