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pactb-loxP-mCherry-loxP-EGFP (#RDB14532)

Expression vector of loxP-mCherry-loxP-EGFP under the medaka (Oryzias latipes) beta-actin promoter.

Clone info. Expression vector of loxP-mCherry-loxP-EGFP under the medaka (Oryzias latipes) beta-actin promoter for the genome engineering. The structure of the plasmid construct, see Fig. 1 of Kishimoto, K. et al., Dev. Growth Differ., 58 (6): 516-521, 2016.
Comment Please refer AB557594 for the parental plasmid beta-act-loxP-RFP-loxP-GFP.
Vector backbone pEGFP-1 (Plasmid)
Size of vector backbone 4.1 kb
Selectable markers Amp^r
Gene/insert name Oryzias latipes actb genomic DNA
Depositor|Developer Kinoshita, Masato |
 

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kishimoto, K. et al. Develop. Growth Differ. 58, 516-521, 2016)。
備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14532 pactb-loxP-mCherry-loxP-EGFP DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pactb-loxP-mCherry-loxP-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14532).

Reference section:

Kishimoto, K., Nakayama, M., Kinoshita, M., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14532_A7Adp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: OI bapro_F ()
Region: OI beta-actin promoter 3', mCherry 5'
Sequence file: RDB14532_A7Ada.seq check
>D04061A1_A7Ad_1_OI bacpro_F_E06_15_ABI24.ab1
    1 CGCAGCAATG GGCTCTGACT GCAAGCAGAT GCAGATGGGC GTGTCTTTGG TGTTAACGCC
   61 TTTTTTGTTT GTTCTTTTCC AGTTTAGCCG TCGACATAAC TTCGTATAGC ATACATTATA
  121 CGAAGTTATA CTAGTACCAT GGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG
  181 GAGTTCATGC GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC
  241 GAGGGCGAGG GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC
  301 AAGGGTGGCC CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC
  361 AAGGCCTACG TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG
  421 GGCTTCAAGT GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG
  481 GACTCCTCCC TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC
  541 CCCTCCGACG GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG
  601 ATGTACCCCG AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC
  661 GGCGGCCACT ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG
  721 CCCGGCGCCT ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC
  781 ATCGTGGAAC AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG
  841 TACAAGTAGC GGCCGCGACT CTAGATCATA ATCAGCCATA CCACATTTGT AGAGGTTTTA
  901 CTTGCTTTAA AAAAACCTCC CACACCTCCC CCCTGAACCT GAAACATAAA ATGAATGCAA
  961 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
 1021 CAAATTTCAC AAATAAAGCA TTTTTTTTTT TCACTGCATT TCTAGTTGTG GGATTTGTCC
 1081 CAAACCTTCA ATCCAAATTG CTATTCTTTA AGACGTAAAT GGTAGCGTAT TAACATATGA
1141 ATATTCCG
//
Primer: mCherry-C_F ()
Region: mCherry 3', EGFP 5'
Sequence file: RDB14532_A7Adb.seq check
>D04061A1 A7Ad-1-mcherry-CF_A03_03_ABI24.ab1
    1 CAGTAGGTCG TGCAGCTGCC CGGCGCCTAC AACGTCAACA TCAAGTTGGA CATCACCTCC
   61 CACAACGAGG ACTACACCAT CGTGGAACAG TACGAACGCG CCGAGGGCCG CCACTCCACC
  121 GGCGGCATGG ACGAGCTGTA CAAGTAGCGG CCGCGACTCT AGATCATAAT CAGCCATACC
  181 ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA
  241 CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA
  301 TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT
  361 GGTTTGTCCA AACTCATCAA TGTATCTTAA GGCGTAAATT GTAAGCGTTA ATTAACATAT
  421 GATATCGATA ACTTCGTATA GCATACATTA TACGAAGTTA TCCGCGGGCC CGGGATCCAC
  481 CGGTCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
  541 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
  601 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC
  661 CCTGCCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CCGCTACCCC
  721 GACCACATGA AGCAGCACGA ACTCTCAGTT CGGCATGGCC CCGTGCTACG TCCAGGAGCG
781 CCACCATTCT TTTCCTTTCC AAGGGGAA
//
Primer: EGFP-N_F ()
Region: EGFP 3'
Sequence file: RDB14532_A7Adc.seq check
>D04061A1 A7Ad-1-EGFP-NF_B03_06_ABI24.ab1
    1 TAGCGAGTCG GCGAGGGCGA GGGCGATGCC ACCTACGGCA AGCTGACCCT GAAGTTCATC
   61 TGCACCACCG GCAAGCTGCC CGTGCCCTGG CCCACCCTCG TGACCACCCT GACCTACGGC
  121 GTGCAGTGCT TCAGCCGCTA CCCCGACCAC ATGAAGCAGC ACGACTTCTT CAAGTCCGCC
  181 ATGCCCGAAG GCTACGTCCA GGAGCGCACC ATCTTCTTCA AGGACGACGG CAACTACAAG
  241 ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC ACCCTGGTGA ACCGCATCGA GCTGAAGGGC
  301 ATCGACTTCA AGGAGGACGG CAACATCCTG GGGCACAAGC TGGAGTACAA CTACAACAGC
  361 CACAACGTCT ATATCATGGC CGACAAGCAG AAGAACGGCA TCAAGGTGAA CTTCAAGATC
  421 CGCCACAACA TCGAGGACGG CAGCGTGCAG CTCGCCGACC ACTACCAGCA GAACACCCCC
  481 ATCGGCGACG GCCCCGTGCT GCTGCCCGAC AACCACTACC TGAGCACCCA GTCCGCCCTG
  541 AGCAAAGACC CCAACGAGAA GCGCGATCAC ATGGTCCTGC TGGAGTTCGT GACCGCCGCC
  601 GGGATCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGCGACTC TAGATCATAA
  661 TCAGCCATAC CACATTTGTA GAGGTTTTAC TTGCTTTAAA AAACCTCCCA CACCTCCCCC
  721 TGAACCTGAA ACATAAAATG ATTGCAGTGT GTGGTAACTG CTTTATTGCA GCTTTATAAT
781 GGTTTACCAA AATTA
//
Primer: SV40pA3'element Reverse ()
Region: ColE1 ori, OI beta-actin promoter 5'
Sequence file: RDB14532_A7Add.seq check
>D04061A1_A7Ad_1_SV40pA3'elementReverse_E02_05_ABI08.ab1
    1 GAGGCAGGGG GGGCGGAGCT ATGGAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG
   61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA
  121 ACCGTATTAC CGCCATGCAT TAGTTATTAC TAGCGCTACC GGACTCAGAT CTCGAGCTCA
  181 AGCTTCGAAT TCAATTACAG TGGTCATGAA TATTATATCT AACCACAGAG CTAAAGAAAA
  241 AACATACCAA GACAGTTTTT TTTCTGTTGA TGGGTGGTCA TTCTACATTG AAGTGAATGG
  301 GGTCTGACTG GTTTGTTTTA CGTGTCAGCT AGTGCTTTCT TGGTGAACTG ATCTGGCTCA
  361 TTTTTTTACT CTGACCTCAT CTGAGAATTG TTCAAAACAA ATGCACGATA TTTAATACTG
  421 CTGATAAGAA GGATTCGCTT CACTTGCAGG TGAACCCTGG TGTGGCAATA GTGTTAATGA
  481 AAACAAACTA CTCGGCAGTA AAAGTATGTG ACATGAATAA AAAAAAATCA CAAACTGGAA
  541 GAAGATCAGA TCATGAAAGA ATTGTAAGAA TGTCCCATTG TACGATGTGT TGTGGTAAAG
  601 TGGTGAAGTC ACTTATCAAT CCATGATGGT CAGAGAACTA GCAGGACTGG TTTTCTTTTC
  661 TTTGTTTTTT TTTATTTCCT TCATCCTGGT ACTGCCCACT TCTGGCCTTC AAACAAAGTC
  721 AAGTATTTTT TTTATTCAAA AGCAGATCAA ATTTAGAAGG TAACATCATC TGCCCATTTG
  781 AATCAAGTCT GTAGAACTAT CTTAGTATGA ACTTGCCATA ATGCTTCATA AGAAGTGATT
  841 GCAAATCACC TACCACACAG CTGGATGAGC TGGAACTCTG ACACATACAT TCATTCACCT
  901 GCAATCACGT AAAGGAGCCT ACTACGAAGG GACACTCCTG TGAGGCAGGA TTACATCCTT
  961 TTCCCAGCCC AACCCCCTGT CTGTGTTTTT CCCAGTTTCA GCAGTCAGTC CTTTCCAGTG
 1021 TGCTCTTACT TTACCAAGAA CCCGGCCCCT CCAGATACTG ACAATAGGAA GTACCATGTG
1081 TTTTTAGAGT GAACGCCCAC CCTTGGAATG GGGAT
//
Primer: SV40pro_F_V2 ()
Region: SV40 promoter/ori, NeoR/KanR
Sequence file: RDB14532_A7Ade.seq check
>D04061A1 A7Ad-1-SV40proFv2_C03_09_ABI24.ab1
    1 AACTTTTTTG GAAGCTAGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG AGGATCGTTT
   61 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA
  121 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG
  181 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA
  241 CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT
  301 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG
  361 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA
  421 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT
  481 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC
  541 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GAGCATGCCC
  601 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA
  661 AATGGCCGCT TTTCTGGATT CATCGACTGT GGGCCGGCTG GGTGTGGCGG ACCGCTATCA
  721 GACATAGCGT TGGGCTACCG TTGATATGCT GAGAGCCTTG GTCGGCCGAA TGGGGCTGAC
781 CGCCTTCGTT CG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kishimoto, K., In vivo recombination efficiency of two site-specific recombination systems, VCre/VloxP and SCre/SloxP, in medaka (Oryzias latipes). Dev. Growth Differ. 58 (6): 516-521 (2016). PMID 27224047. [link to RRC of NBRP]