RDB14520 - pGL4_ifng-36-luc

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pGL4_ifng-36-luc

Reporter construct of mouse interferon gamma (Ifng) promoter (-36 to +113).

Catalog number	RDB14520
Resource name	pGL4_ifng-36-luc
Clone info.	Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-36 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment	The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-36-luc, the PCR product (0.1 kb) was amplified with forward primer (5' CTA CTC GAG TTC GAT CAG GTA TAA AAC TGG 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector.
Vector backbone	pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	mouse Ifng genomic DNA
Depositor\|Developer	Kataoka, Takao \|

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mouse Ifng

External Database
mouse Ifng

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14520	pGL4_ifng-36-luc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

Further references such as user reports and related articles (go to bottom)

Featured content	T cell receptor signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14520_A6Lfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert Sequence file: RDB14520_A6Lfa.seq
>D03972A6_A6Lf_1_pGL4-4174F_G08_07_ABI08.ab1 1 ATGAGGGTCC CAGGACATTT CTCTGGCTAA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA 61 GTTCGATCAG GTATAAAACT GGAAGCCAGA GAGGTGCAGG CTATAGCTGC CATCGGCTGA 121 CCTAGAGAAG ACACATCAGC TGATCCTTTG GACCCTCTGA CTTGAGACAG AAGTTCTGGG 181 CTTCTCCTCC TGCGGCCTAG CTCTGAGACA GATATCTAGA TCAAGATCTG GCCTCGGCGG 241 CCAAGCTTGG CAATCCGGTA CTGTTGGTAA AGCCACCATG GAAGATGCCA AAAACATTAA 301 GAAGGGCCCA GCGCCATTCT ACCCACTCGA AGACGGGACC GCCGGCGAGC AGCTGCACAA 361 AGCCATGAAG CGCTACGCCC TGGTGCCCGG CACCATCGCC TTTACCGACG CACATATCGA 421 GGTGGACATT ACCTACGCCG AGTACTTCGA GATGAGCGTT CGGCTGGCAG AAGCTATGAA 481 GCGCTATGGG CTGAATACAA ACCATCGGAT CGTGGTGTGC AGCGAGAATA GCTTGCAGTT 541 CTTCATGCCC GTGTTGGGTG CCCTGTTCAT CGGTGTGGCT GTGGCCCCAG CTAACGACAT 601 CTACAACGAG CGCGAGCTGC TGAACAGCAT GGGCATCAGC CAGCCCACCG TCGTATTCGT 661 GAGCAAGAAA GGGCTGCAAA AGATCCTCAA CGTGCAAAAG AAGCTACCGA TCATACAAAA 721 GATCATCATC ATGGATAGCA AGACCGACTA CCAGGGCTTC CAAAGCATGT ACACCTTCGT 781 GACTTCCCAT TTGCCACCCG GCTTCAACGA GTACGACTTC GTGCCCGAGA GCTTCGACCG 841 GGACAAAACC ATCGCCCTGA TCATGAACAG TAGTGGCAGT ACCGGATTGC CCAAGGGGCG 901 TAGCCCCTAC CGCACCGCAC CGCTTGTGTC CGATTTCAGT CATGCCCGCG ACCCCCATCT 961 TCGGGCAACC AGATCATCCC CGACACCGCT ATCCCTCAGC GTGGTGCCAT TTCACCCACG 1021 GCTTTCGCAT GTTTCCACCA CGCTGGGCTA CTTGATCCTG CCGGCTTTCG GGGTCGTGCT 1081 TCATGTAACG CTTCGGAGGC AGAGCTAATT CCGTGCCTAG CTGGCAGACC TAATAGAG //
Primer: SV40pro_R2 Region: Luciferase 3' Sequence file: RDB14520_A6Lfb.seq
>D03972A6_A6Lf_1_SV40pro_R2_H09_24_ABI24.ab1 1 AGCAAGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA 61 CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGAAGGTAG CTAACCAAGT TCCTCTTTCA 121 GAGGTTATTT CAGGCCATGG TGCTGCGCAG ATCAGCGGAA GAGCGCCCAA TACGCAAACG 181 GATCCTTATC GATTTTACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA ACCTCCCACA 241 CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT TGTTTATTGC 301 AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA AAGCATTTTT 361 TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTATC ATGTCTGCTC 421 GAAGCGGCCG GCCGCCCCGA CTCTAGACTG CAGTTAGACG TTGATCCTGG CGCTGGCGCA 481 AGCAGCAGGG TGTCTATCCA TGCCGCTCTC CTGGGCGCAG CTCATGGGCA GGGTGCCGGC 541 GGCCTGCTCC TCCACCTCGG GAGGGAAGCC GTGGCTGTTA AGGTGGATCA CAAAGTGGCT 601 TAAGCTACTG AACCAGTTCT TGCAAGCAGA ATTCACGGCG ATCTTGCCGC CCTTCTTGGC 661 CTTAATGAGA ATCTCGCGGA TCTTGCGGGC GTCCAACTTG CCGGTCAGTC CTTTAGGCAC 721 CTCGTCCACG AACACAACAC CACCGCGCAG CTTCTTGGCG GTTGTAACCT GGCTGGCCAC 781 ATAGTCCACG ATCTCCTTCT CGGTCATGGT TTTACCGTGT TCCAGCACGA CGACTGCGGC 841 GGGCAGCTCG CCGGCATCGT CGTCGGGCAG GCCGGCGACC CCGGCGTCGA AGATGTTGGG 901 GTGTTGCAGC AGATGCTCTC CAGTTCGGCT GGGGCTACCT GGTAGCCCTT GTATTTGATC 961 AGGCTCTTCA GCCCGGTCCA CGATGAAGAA GTGCTCGTCC TCGTCCCAGT AGGCGATGTC 1021 GCCGCTGTGC AGCCAGCCGT CCTTGTCGAT GAGAGCGTTT GTAGCCCTCC GGGGATGTAC 1081 GTAGCCGCTC ATGAATCATG GGGCACGAAC GCCACAGCTT CGGCCGCGCT TGGAATCACA 1141 CACCCCAGTG C //
Primer: SV40pro_F Region: SV40 pro/ori, PuroR, poly(A) signal Sequence file: RDB14520_A6Lfc.seq
>D03972A6_A6Lf_1_SV40pro_F_C09_03_ABI08.ab1 1 CCCTCGGTCC CTGGGCATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC 61 AAAAAGCTCG ATTCTTCTGA CACTAGCGCC ACCATGACCG AGTACAAGCC TACCGTGCGC 121 CTGGCCACTC GCGATGATGT GCCCCGCGCC GTCCGCACTC TGGCCGCCGC TTTCGCCGAC 181 TACCCCGCTA CCCGGCACAC CGTGGACCCC GACCGGCACA TCGAGCGTGT GACAGAGTTG 241 CAGGAGCTGT TCCTGACCCG CGTCGGGCTG GACATCGGCA AGGTGTGGGT AGCCGACGAC 301 GGCGCGGCCG TGGCCGTGTG GACTACCCCC GAGAGCGTTG AGGCCGGCGC CGTGTTCGCC 361 GAGATCGGCC CCCGAATGGC CGAGCTGAGC GGCAGCCGCC TGGCCGCCCA GCAGCAAATG 421 GAGGGCCTGC TTGCCCCCCA TCGTCCCAAG GAGCCTGCCT GGTTTCTGGC CACTGTAGGA 481 GTGAGCCCCG ACCACCAGGG CAAGGGCTTG GGCAGCGCCG TCGTGTTGCC CGGCGTAGAG 541 GCCGCCGAAC GCGCCGGTGT GCCCGCCTTT CTCGAAACAA GCGCACCAAG AAACCTTCCA 601 TTCTACGAGC GCCTGGGCTT CACCGTGACC GCCGATGTCG AGGTGCCCGA GGGACCTAGG 661 ACCTGGTGTA TGACACGAAA ACCTGGCGCC TAATGATCTA GAACCGGTCA TGGCCGCAAT 721 AAAATATCTT TATTTTCATT ACATCTGTGT GTTGGTTTTT TGTGTGTTCG AACTAGATGC 781 TGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC 841 ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG 901 CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 961 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 1021 CGCAGGAAAG ACATGTGAGC AAAAGGCCAG CAAAGGCCAG GACCGTAAAA GGCCGCGTGC 1081 TGCGTTTTTC ATAGCTCGCC CCCTGACGAG CATCCAAAAT CGAACGCCTC AGTCGAGGTG 1141 GCCGAACCGA CATGACTATA AAGAATACCA AGGGCGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl