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pMXs-IRES-hNGFR_G-CaMP6

Retroviral expression vector of calcium reproter G-CaMP6.

Catalog number RDB14513
Resource name pMXs-IRES-hNGFR_G-CaMP6
Clone info. Retroviral expression vector of calcium reproter G-CaMP6, cloned into the pMXs-IRES-hNGFR. Refer Fig.S1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-hNGFR (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human NGFR cDNA Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kitamura, Daisuke |
Other clones in our bank

External Database
human NGFR Rat Calm2 Chickin MLCK Aequorea victoria GFP

      Reference sequence
        

      Distribution information

      Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
      Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
      Additional terms and conditions for distribution Regarding G-CaMP6: (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Ohkura, M., PLoS One 7 (12): e51286, 2012) and an acknowledgment are requested. (2) The DEVELOPER may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEVELOPER. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

      Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
      Ordering Please visit Information of Request for Distribution.[link] 
      Order form 
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      Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
      This RESOURCE contains a fluorescent protein owned or licensed by Global Life Sciences Solutions Operations UK Ltd. (Cytiva). Please refer to Schedule B and use Schedule A (download the Schedule A and Schedule B [link]).
      Remarks Remember that you will be working with samples containing infectious virus.
      提供案内 (日本国内) [open/close]

      提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) を引用し、寄託者への謝辞の表明を必要とする。
      付加的提供条件 G-CaMP6について:(1) 利用者は、研究成果の公表にあたって 文献 (Ohkura, M., PLoS One 7 (12): e51286, 2012) を引用し、謝辞の表明を必要とする。(2) 開発者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に開発者の求めに対して回答することとする。(3) 当該リソースの営利目的での利用(営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同 研究、非営利団体による特許取得・ライセンス契約等の行為等)の希望については、事前に 寄託者との間で、別途使用許諾に係る契約の締結が必要である。
      pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
      提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
      提供依頼書 [Word]
      提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
      GFPリソースを含みます。Schedule Bをご一読の上Schedule A (英語版のみ) の提出をお願いします (Schedule AとSchedule Bを入手[link])。
      備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

      Catalog # Resource name Shipping form Fee (non-profit org.)
      RDB14513 pMXs-IRES-hNGFR_G-CaMP6 DNA solution

      Please review the QC test results indicated by check icon below as well as clone information before placing your order.

      References and tips

      Electronic file

      Original reference

      original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

      Further references such as user reports and related articles (go to bottom)

      Featured content

      Featured content Calcium Ion Sensor Clones (English text)

      Sequence information

      RIKEN BRC has sequenced portions of this material for quality test.
      Please review the QC test results indicated by check icon as well as clone information before placing your order.

      Test sheet RDB14513_A6Lep1.pdf check

      Nucleotide sequence of a portion of this resource (if available).

      Primer: pBABE5'
      Sequence file: RDB14513_A6Lea.seq check
      >D04073B4_A6Le_1_pBABE5'_G01_19_ABI24.ab1
          1 GTCCTTATAT GGCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
         61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
        121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
        181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
        241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
        301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
        361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTCGCCAC CATGCGGGGT TCTCATCATC
        421 ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG
        481 ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC ACGTCGTAAG TGGAATAAGA
        541 CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT CGAGAACGTC TATATCAAGG
        601 CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
        661 GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
        721 TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT TTCGAAAGAC CCCAACGAGA
        781 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
        841 ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG CAAGGGCGAG GAGCTGTTCA
        901 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
        961 TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
       1021 CCACCCGGCA AGCTGCCCGT GCCCTGGCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
       1081 AGTGCCTTCA GCCGCTACCC CGACACATGA GGCCAGCACG ACCTCTCAGT CGGTCATGCC
      1141 CGAAGCTACA GTCCACGAAG CGCAAGCAAT CGCTG
      //
      Primer: IRES-R
      Sequence file: RDB14513_A6Leb.seq check
      >D04073B4_A6Le_1_IRES-R_E03_15_ABI24.ab1
          1 GCATCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
         61 ATTTACGTAG CGGCCGCTCA CTTCGCTGTC ATCATTTGTA CAAACTCTTC GTAGTTTACC
        121 TGACCATCCC CATCGATGTC TGCTTCCCTG ATCATTTCAT CAACCTCTTC ATCTGTTAAC
        181 TTCTCTCCAA GGTTTGTCAT CACGTGGCGA AGCTCTGCTG CACTGATGTA GCCATTGCCA
        241 TCCTTATCAA ACACACGGAA CGCTTCTCTA ATTTCTTCTT CACTGTCTGT GTATTTCATT
        301 TTTCTTGCCA TCATTGTCAG GAACTCAGGG AAGTCGATTG TGCCATCACC GTCGGCATCT
        361 ACTTCATTGA TCATGTCCTG CAGCTCTGCT TCTGTGGGGT TCTGCCCCAG AGACCGCAGC
        421 ACCGTCCCCA GCTCCTTGGT TGTTATTGTC CCATCCCCGT CCTTGTCAAA TAGGGAGAAA
        481 GCCTCTTTAA ATTCTGCGAT CTGCTCTTCA GTCAGTTGGT CACGCGTGTT GTACTCCAGC
        541 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCAC GATGCGGTTC
        601 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTAGCC GTCGTCCTTG
        661 AAGAAGATGG TGCGCTCCTG GATGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
        721 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG
        781 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG
        841 CCGTAGGTGG CATCGCCCTC ACCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
        901 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
        961 ATGCTCCCTC CGGTACCGCC CTTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGGCGG
       1021 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTGGGGTCTT TCGAAGTTTG
       1081 ACTGCACGCT CAGTAGTTGA TTGTCAGCAG CCAGCACGGG GCCGTTCGCC GAATGGGGTG
      1141 TTCCTGCTGT ATGTAGACAG CCTGCACGGA CCCG
      //
      Primer: IRES-F
      Sequence file: RDB14513_A6Lec.seq check
      >D04073B4_A6Le_1_IRES-F_H10_22_ABI24.ab1
          1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
         61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGA CGGGCCGCGC
        121 CTGCTGCTGT TGCTGCTTCT GGGGGTGTCC CTTGGAGGTG CCAAGGAGGC ATGCCCCACA
        181 GGCCTGTACA CACACAGCGG TGAGTGCTGC AAAGCCTGCA ACCTGGGCGA GGGTGTGGCC
        241 CAGCCTTGTG GAGCCAACCA GACCGTGTGT GAGCCCTGCC TGGACAGCGT GACGTTCTCC
        301 GACGTGGTGA GCGCGACCGA GCCGTGCAAG CCGTGCACCG AGTGCGTGGG GCTCCAGAGC
        361 ATGTCGGCGC CGTGCGTGGA GGCCGACGAC GCCGTGTGCC GCTGCGCCTA CGGCTACTAC
        421 CAGGATGAGA CGACTGGGCG CTGCGAGGCG TGCCGCGTGT GCGAGGCGGG CTCGGGCCTC
        481 GTGTTCTCCT GCCAGGACAA GCAGAACACC GTGTGCGAGG AGTGCCCCGA CGGCACGTAT
        541 TCCGACGAGG CCAACCACGT GGACCCGTGC CTGCCCTGCA CCGTGTGCGA GGACACCGAG
        601 CGCCAGCTCC GCGAGTGCAC ACGCTGGGCC GACGCCGAGT GCGAGGAGAT CCCTGGCCGT
        661 TGGATTACAC GGTCCACACC CCCAGAGGGC TCGGACAGCA CAGCCCCCAG CACCCAGGAG
        721 CCTGAGGCAC CTCCAGAACA AGACCTCATA GCCAGCACGG TGGCAGGTGT GGTGACCACA
        781 GTGATGGGCA GCTCCCAGCC CGTGGTGACC CGAGGCACCA CCGACAACCT CATCCCTGTC
        841 TATTGCTCCA TCCTGGCTGC TGTGGTTGTG GGCCTTGTGG CCTACATAGC CTTCAAGAGG
        901 TGGAACAGCT GCAAGCAGAA CAAGCAAGGA GCCAACAGCT GAGTCGACGA TAAATAAAAG
        961 ATTTTATTTA GTCTCCAGAA AAAGGGGGGA AATGAAAGAC CCCACCCTGT AGTTTGGGCA
       1021 AGGCTAGCTT AGTACGGCCA ATTTTTGGCA AGCAATGAAA AATTACATTA ACCTTGAGAT
      1081 TAGAGAGTCC AGACTCAAGT CAAGAACAGA ATTGGAACCA
      //
      Primer: Amp_R
      Sequence file: RDB14513_A6Led.seq check
      >D04073B4_A6Le_1_Amp_R_F11_17_ABI24.ab1
          1 ATTAGTTTTT CATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
         61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
        121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
        181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
        241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
        301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
        361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
        421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
        481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
        541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
        601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT
        661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT
        721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG
        781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA
        841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG
        901 TGGAACTGAC GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG
        961 GGGCCGTTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG ACTCTTTTGG
       1021 GTGCACCCCC CCTTTAGAGG AGGGATAATG TGGTTTCTGT AGAGACGAAG AACTTAAACA
      1081 GTCAGCCTTC GGTCTGATTT TTTGGCCTTT CCGGATTGGG GGAATC
      //
      Primer: SV40pA3'element Reverse
      Sequence file: RDB14513_A6Lee.seq check
      >D04073B4_A6Le_1_SV40pA3element Reverse_G11_20_ABI24.ab1
          1 GCGCGGCCAG GGGGGCGGAG CCTATGGAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
         61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
        121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
        181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
        241 GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG TAAATACTTA AGAAAAAAAA
        301 CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC GCTGACGGGT AGTCAATCAC
        361 TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC GGATGCAACT GCAAGAGGGT
        421 TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG AGGACTGGCG CCCCGAGTGA
        481 GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC GCGCGAACAG AAGCGAGAAG
        541 CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT CAGGTCCTTG GGGCACCCTG
        601 GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA CCGCATCTGG GGACCATCTG
        661 TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA TATCCTGTTT GGCCCATATT
        721 CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA ACTGCTTACC ACAGATATCC
        781 TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT TGATCTGAAC TTCTCTATTC
        841 TCAGTTATGT ATTTTTTCCA TGCCTTGCAA AATGGCGTTA CTTAAGCTAG CTTGCCAAAC
        901 CTACAGGTGG GGTCTTTCAT TCCCCCCCTT TTTCTGGAGA CTAAATAAAA TCTTTTTATT
        961 TTATCGTCGA CTCAGCTGTT GGGCTCCTTG CTTTGTTTCT GCTTGCAGCT GTTCCAACCT
       1021 CTGAAGCTAT GTAGGGCCAC AAGCTCCACA ACCACCAGCA GCCAAGGATG AGCCAATAGA
      1081 CAGGGAATGA ACGTTGGTCC CGGATGAGGT TTGAACT
      //

      Please visit Sequencing and PCR primers for primer information.


      References

      Original, user report and related articles

      original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

      2022.05.17

      GNP_filter3_RDBDEP_html_220516.pl