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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-hNGFR_G-CaMP6 (#RDB14513)

Retroviral expression vector of calcium reproter G-CaMP6.

Clone info. Retroviral expression vector of calcium reproter G-CaMP6, cloned into the pMXs-IRES-hNGFR. Refer Fig.S1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-hNGFR (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name human NGFR cDNA Rat Calm2 cDNA Chickin MLCK cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding G-CaMP6: (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature (Ohkura, M., PLoS One 7 (12): e51286, 2012) and an acknowledgment are requested. (2) The DEVELOPER may request the RECIPIENT to report on progress and/or results obtained through the use of the BIOLOGICAL RESOURCE, and the RECIPIENT shall respond truthfully to such a request by the DEVELOPER. (3) Any use of the BIOLOGICAL RESOURCE for profit purposes by the RECIPIENT, such as any use by for-profit entities, any use for the BIOLOGICAL RESOURCE by not-for-profit entities in researches funded in any part by or in collaboration with for-profit entities, or any use the BIOLOGICAL RESOURCE by the not-for-profit entities for patent filing or licensing, requires a separate license from the DEPOSITOR, prior to distribution.

Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
G-CaMP6について：(1) 利用者は、研究成果の公表にあたって 文献 （Ohkura, M., PLoS One 7 (12): e51286, 2012) を引用し、謝辞の表明を必要とする。(2) 開発者は、利用の状況及び成果等について利用者に報告を求めることができ、利用者は誠実に開発者の求めに対して回答することとする。(3) 当該リソースの営利目的での利用（営利団体による利用、非営利団体が営利団体から直接・間接を問わず資金等を受けて実施する研究、非営利団体と営利団体との共同 研究、非営利団体による特許取得・ライセンス契約等の行為等）の希望については、事前に 寄託者との間で、別途使用許諾に係る契約の締結が必要である。
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14513 pMXs-IRES-hNGFR_G-CaMP6 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-hNGFR_G-CaMP6 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14513).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14513_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14513_A6Lea.seq check
>D04073B4_A6Le_1_pBABE5'_G01_19_ABI24.ab1
    1 GTCCTTATAT GGCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
   61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
  121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
  181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
  241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
  301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
  361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCTCGCCAC CATGCGGGGT TCTCATCATC
  421 ATCATCATCA TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG
  481 ACGATGACGA TAAGGATCTC GCCACCATGG TCGACTCATC ACGTCGTAAG TGGAATAAGA
  541 CAGGTCACGC AGTCAGAGCT ATAGGTCGGC TGAGCTCACT CGAGAACGTC TATATCAAGG
  601 CCGACAAGCA GAAGAACGGC ATCAAGGCGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
  661 GCGGCGTGCA GCTCGCCTAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
  721 TGCTGCCCGA CAACCACTAC CTGAGCGTGC AGTCCAAACT TTCGAAAGAC CCCAACGAGA
  781 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
  841 ACGAGCTGTA CAAGGGCGGT ACCGGAGGGA GCATGGTGAG CAAGGGCGAG GAGCTGTTCA
  901 CCGGGGTGGT GCCCATCCTG GTCGAGCTGG ACGGCGACGT AAACGGCCAC AAGTTCAGCG
  961 TGTCCGGCGA GGGTGAGGGC GATGCCACCT ACGGCAAGCT GACCCTGAAG TTCATCTGCA
 1021 CCACCCGGCA AGCTGCCCGT GCCCTGGCCA CCCTCGTGAC CACCCTGACC TACGGCGTGC
 1081 AGTGCCTTCA GCCGCTACCC CGACACATGA GGCCAGCACG ACCTCTCAGT CGGTCATGCC
1141 CGAAGCTACA GTCCACGAAG CGCAAGCAAT CGCTG
//
Primer: IRES-R
Sequence file: RDB14513_A6Leb.seq check
>D04073B4_A6Le_1_IRES-R_E03_15_ABI24.ab1
    1 GCATCAGCGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCTCA CTTCGCTGTC ATCATTTGTA CAAACTCTTC GTAGTTTACC
  121 TGACCATCCC CATCGATGTC TGCTTCCCTG ATCATTTCAT CAACCTCTTC ATCTGTTAAC
  181 TTCTCTCCAA GGTTTGTCAT CACGTGGCGA AGCTCTGCTG CACTGATGTA GCCATTGCCA
  241 TCCTTATCAA ACACACGGAA CGCTTCTCTA ATTTCTTCTT CACTGTCTGT GTATTTCATT
  301 TTTCTTGCCA TCATTGTCAG GAACTCAGGG AAGTCGATTG TGCCATCACC GTCGGCATCT
  361 ACTTCATTGA TCATGTCCTG CAGCTCTGCT TCTGTGGGGT TCTGCCCCAG AGACCGCAGC
  421 ACCGTCCCCA GCTCCTTGGT TGTTATTGTC CCATCCCCGT CCTTGTCAAA TAGGGAGAAA
  481 GCCTCTTTAA ATTCTGCGAT CTGCTCTTCA GTCAGTTGGT CACGCGTGTT GTACTCCAGC
  541 TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCAC GATGCGGTTC
  601 ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTAGCC GTCGTCCTTG
  661 AAGAAGATGG TGCGCTCCTG GATGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC
  721 TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG
  781 AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG
  841 CCGTAGGTGG CATCGCCCTC ACCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
  901 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
  961 ATGCTCCCTC CGGTACCGCC CTTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGGCGG
 1021 CGGTCACGAA CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTGGGGTCTT TCGAAGTTTG
 1081 ACTGCACGCT CAGTAGTTGA TTGTCAGCAG CCAGCACGGG GCCGTTCGCC GAATGGGGTG
1141 TTCCTGCTGT ATGTAGACAG CCTGCACGGA CCCG
//
Primer: IRES-F
Sequence file: RDB14513_A6Lec.seq check
>D04073B4_A6Le_1_IRES-F_H10_22_ABI24.ab1
    1 TGCATCATTT ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGA CGGGCCGCGC
  121 CTGCTGCTGT TGCTGCTTCT GGGGGTGTCC CTTGGAGGTG CCAAGGAGGC ATGCCCCACA
  181 GGCCTGTACA CACACAGCGG TGAGTGCTGC AAAGCCTGCA ACCTGGGCGA GGGTGTGGCC
  241 CAGCCTTGTG GAGCCAACCA GACCGTGTGT GAGCCCTGCC TGGACAGCGT GACGTTCTCC
  301 GACGTGGTGA GCGCGACCGA GCCGTGCAAG CCGTGCACCG AGTGCGTGGG GCTCCAGAGC
  361 ATGTCGGCGC CGTGCGTGGA GGCCGACGAC GCCGTGTGCC GCTGCGCCTA CGGCTACTAC
  421 CAGGATGAGA CGACTGGGCG CTGCGAGGCG TGCCGCGTGT GCGAGGCGGG CTCGGGCCTC
  481 GTGTTCTCCT GCCAGGACAA GCAGAACACC GTGTGCGAGG AGTGCCCCGA CGGCACGTAT
  541 TCCGACGAGG CCAACCACGT GGACCCGTGC CTGCCCTGCA CCGTGTGCGA GGACACCGAG
  601 CGCCAGCTCC GCGAGTGCAC ACGCTGGGCC GACGCCGAGT GCGAGGAGAT CCCTGGCCGT
  661 TGGATTACAC GGTCCACACC CCCAGAGGGC TCGGACAGCA CAGCCCCCAG CACCCAGGAG
  721 CCTGAGGCAC CTCCAGAACA AGACCTCATA GCCAGCACGG TGGCAGGTGT GGTGACCACA
  781 GTGATGGGCA GCTCCCAGCC CGTGGTGACC CGAGGCACCA CCGACAACCT CATCCCTGTC
  841 TATTGCTCCA TCCTGGCTGC TGTGGTTGTG GGCCTTGTGG CCTACATAGC CTTCAAGAGG
  901 TGGAACAGCT GCAAGCAGAA CAAGCAAGGA GCCAACAGCT GAGTCGACGA TAAATAAAAG
  961 ATTTTATTTA GTCTCCAGAA AAAGGGGGGA AATGAAAGAC CCCACCCTGT AGTTTGGGCA
 1021 AGGCTAGCTT AGTACGGCCA ATTTTTGGCA AGCAATGAAA AATTACATTA ACCTTGAGAT
1081 TAGAGAGTCC AGACTCAAGT CAAGAACAGA ATTGGAACCA
//
Primer: Amp_R
Sequence file: RDB14513_A6Led.seq check
>D04073B4_A6Le_1_Amp_R_F11_17_ABI24.ab1
    1 ATTAGTTTTT CATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
   61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
  121 CCACCTGCAT AATGAAAGAC CCCACCTGTA GGTTTGGCAA GCTAGCTTAA GTAACGCCAT
  181 TTTGCAAGGC ATGGAAAAAT ACATAACTGA GAATAGAAAA GTTCAGATCA AGGTCAGGAA
  241 CAGATGGAAC AGCTGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT TCCTGCCCCG
  301 GCTCAGGGCC AAGAACAGAT GGAACAGCTG AATATGGGCC AAACAGGATA TCTGTGGTAA
  361 GCAGTTCCTG CCCCGGCTCA GGGCCAAGAA CAGATGGTCC CCAGATGCGG TCCAGCCCTC
  421 AGCAGTTTCT AGAGAACCAT CAGATGTTTC CAGGGTGCCC CAAGGACCTG AAATGACCCT
  481 GTGCCTTATT TGAACTAACC AATCAGTTCG CTTCTCGCTT CTGTTCGCGC GCTTCTGCTC
  541 CCCGAGCTCA ATAAAAGAGC CCACAACCCC TCACTCGGCG CGCCAGTCCT CCGATTGACT
  601 GAGTCGCCCG GGTACCCGTG TATCCAATAA ACCCTCTTGC AGTTGCATCC GACTTGTGGT
  661 CTCGCTGTTC CTTGGGAGGG TCTCCTCTGA GTGATTGACT ACCCGTCAGC GGGGGTCTTT
  721 CATTTGGGGG CTCGTCCGGG ATCGGGAGAC CCCTGCCCAG GGACCACCGA CCCACCACCG
  781 GGAGGTAAGC TGGCCAGCAA CTTATCTGTG TCTGTCCGAT TGTCTAGTGT CTATGACTGA
  841 TTTTATGCGC CTGCGTCGGT ACTAGTTAGC TAACTAGCTC TGTATCTGGC GGACCCGTGG
  901 TGGAACTGAC GAGTTCGGAA CACCCGGCCG CAACCCTGGG AGACGTCCCA GGGACTTCGG
  961 GGGCCGTTTT TTGTGGCCCG ACCTGAGTCC AAAAATCCCG ATCGTTTTGG ACTCTTTTGG
 1021 GTGCACCCCC CCTTTAGAGG AGGGATAATG TGGTTTCTGT AGAGACGAAG AACTTAAACA
1081 GTCAGCCTTC GGTCTGATTT TTTGGCCTTT CCGGATTGGG GGAATC
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14513_A6Lee.seq check
>D04073B4_A6Le_1_SV40pA3element Reverse_G11_20_ABI24.ab1
    1 GCGCGGCCAG GGGGGCGGAG CCTATGGAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
   61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
  121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
  181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCCAATA CGCAAACCGC CTCTCCCCGC
  241 GCGTTGGCCG ATTCATTAAT GCAACTATGG CCATTTAATG TAAATACTTA AGAAAAAAAA
  301 CCAAATTAAT TTTGATACAT GCTGCATGTG AAAGACCCCC GCTGACGGGT AGTCAATCAC
  361 TCAGAGGAGA CCCTCCCAAG GAACAGCGAG ACCACAAGTC GGATGCAACT GCAAGAGGGT
  421 TTATTGGATA CACGGGTACC CGGGCGACTC AGTCAATCGG AGGACTGGCG CCCCGAGTGA
  481 GGGGTTGTGG GCTCTTTTAT TGAGCTCGGG GAGCAGAAGC GCGCGAACAG AAGCGAGAAG
  541 CGAACTGATT GGTTAGTTCA AATAAGGCAC AGGGTCATTT CAGGTCCTTG GGGCACCCTG
  601 GAAACATCTG ATGGTTCTCT AGAAACTGCT GAGGGCTGGA CCGCATCTGG GGACCATCTG
  661 TTCTTGGCCC TGAGCCGGGG CAGGAACTGC TTACCACAGA TATCCTGTTT GGCCCATATT
  721 CAGCTGTTCC ATCTGTTCTT GGCCCTGAGC CGGGGCAGGA ACTGCTTACC ACAGATATCC
  781 TGTTTGGCCC ATATTCAGCT GTTCCATCTG TTCCTGACCT TGATCTGAAC TTCTCTATTC
  841 TCAGTTATGT ATTTTTTCCA TGCCTTGCAA AATGGCGTTA CTTAAGCTAG CTTGCCAAAC
  901 CTACAGGTGG GGTCTTTCAT TCCCCCCCTT TTTCTGGAGA CTAAATAAAA TCTTTTTATT
  961 TTATCGTCGA CTCAGCTGTT GGGCTCCTTG CTTTGTTTCT GCTTGCAGCT GTTCCAACCT
 1021 CTGAAGCTAT GTAGGGCCAC AAGCTCCACA ACCACCAGCA GCCAAGGATG AGCCAATAGA
1081 CAGGGAATGA ACGTTGGTCC CGGATGAGGT TTGAACT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]