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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgA (#RDB14507)

Retroviral expression vector of mouse Immunoglobulin A (IgA).

Clone info. Retroviral expression vector of mouse Immunoglobulin A (IgA), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Igha cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14507 pMXs-IRES-GFP_IgA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14507).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14507_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14507_A6Lea.seq check
>D04073A7_A6Le_1_pBABE5'_A10_01_ABI24.ab1
    1 GCGATATGGA CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
   61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
  121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
  181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
  241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
  301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
  361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCCAGTGTGG TGGTACGGGA ATTCACACAG
  421 GACCTCACCA TGGGATGGAG CTGTATCATG CTCTTCTTGG CAGCAACAGC TACAGGTGTC
  481 CACTCCCAGG TCCAACTGCA GCAGCCTGGG GCTGAGCTTG TGAAGCCTGG GGCTTCAGTG
  541 AAGCTGTCCT GCAAGGCTTC TGGCTACACC TTCACCAGCT ACTTGATGCA CTGGGTGAAG
  601 CAGAGGCCTG GACGAGGCCT TGAGTGGATT GGAAGGATTG ATCCTAATAG TGGTGGTACT
  661 AAGTACAATG AGAAGTTCAA GAGCAAGGCC ACACTGACTG TAGACAAACC CTCCAGCACA
  721 GCCTACATGC AGCTCAGCAG CCTGACATCT GAGGACTCTG CGGTCTATTA TTGTGCAAGA
  781 TATGATTACT ACGGTAGTAG CTACTTTGAC TACTGGGGCC AAGGCACCAC TCTCACAGTC
  841 TCGAGTGAGT CTGCGAGAAA TCCCACCATC TACCCACTGA CACTCCCACG AGCTCTGTCA
  901 AGTGACCCAG TGATAATCGG GCTGCCTGAT TCACGATTAC TTCCCTTCCC GGCACGATGA
  961 ATGTGACCTG GGGAAAAGAG TGGAAGGATA TAACCACCGT AAACTTCCCA CCTGCCCTGG
 1021 GCCTCTGGGG GAGGTACACC CATGAAGCAG CCAGCTGACC CTGCCAGCTG TCGGAGTGCC
1081 AGAAGAGATT CGTGCAATGT TTCGGTGCAA ACAATGAGAC CTCCCT
//
Primer: IRES-R
Sequence file: RDB14507_A6Leb.seq check
>D04073A7_A6Le_1_IRES-R_G02_20_ABI24.ab1
    1 GCATCAGCGG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCTCA GTACTGGGGG ACCTCTTTGC TGCCAAACGG GCCTCGAACA
  121 GTTGTAACAG TGAGTGCTGT GCTGTAGAAC AAGCTCAGTA GGAAGAGGGT GAGGAAGGTC
  181 ACAGTGGTGG GCCACAGGCT GGCACCTGGG GCCTCTTCCT CCAGGATGTC TTCTGACTGG
  241 TCCAGTAGCA CATAGGAAAG TGGCTCTTGA CGTTCCGACA GACGGTCGAT GGTCTTCTGG
  301 GTGAAGTTCA TGGGCAAGGC CTCGTGGCCC ACCATGCAGG AGTACTGGTC ACCCTGTTTC
  361 CAGAGTTCAG CTGATACACG CAACACGCTT GTCACCAGGT AGGTGGTGGC TCCCTCGCCT
  421 GGCTCCTTTA GGGGCTCAAA CACTAGGTAG CTTTCTGGGG ACAGCTCCTC ATTTCCATGC
  481 AGCCATCGCA CCAGCACTTC TTTAGGGTTG AAAGCTCGCA CCAGGCATGT CAGGGACACG
  541 AGCTCATTCA GGGCCAGCTC CTCCGACGGC GGCGGTAGCA GGTGGACCTG GGGTGGGAAG
  601 GTGTTCACTG TGATTTTGGC AATTGTGCCA GTTAAGGTGT CAGACTCAGG ATGGGTAACT
  661 GTGCACTTGA ATGATGCGCC ACTGTTCCAG CGCTCAGCAC AGCCAGGCAG GACGCTGGAC
  721 ACACTGTAGC AGCCGCAGGA ATTCTGCACA GCTTTCTTCT GCACTGCATC CTTCCCAGTG
  781 GAGGGCTCCC AGGTGAAGAC AGCTCCCTCA GGATTTCTCA GGCCATTCAG AGTACATGTG
  841 AGGCTGGCAT CTGAACCCAG GAGCAGGTCC TCAAGAGCTG GCCGCTGCAG TGACAGGCTG
  901 GGATGGCAGG AAGGAGGACA AGGAGGACAA GGAGGAGGAG GACCAGAGCA CTTCACATCC
  961 AATTCTTGGA CGGCGTTAGA GTCATGTTGC ACGGAACATT TCACGGGATT CTCCTTCTGG
 1021 CACTCGACAG CTGGCAGGTC AACTGCTGCT CATGGTGTAC CCTCCCTCCA GAGCAAGGCA
 1081 AGTGAGGTTA CGTGTATATC GTTCCACTCT TTCCCAAGTC ACATTCATCG TTGCCGGAAA
//
Primer: IRES-F
Sequence file: RDB14507_A6Lec.seq check
>D04073A7_A6Le_1_IRES-F_C10_07_ABI24.ab1
    1 GGAAAGGCTT TATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
   61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
  121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
  181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
  241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
  301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
  361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
  421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
  481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
  541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
  601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
  661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
  721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
  781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
  841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTTATTTAGT CTCCAGAAAA AGGGGGGGGA
  901 ATGAAAGACC CCCACCTGTA GGTTTTGGCA AGCTAGCTTA AAGTTAACGC CCATTTTTGC
  961 AAGGGCATGG AAAAAATACA TTAACTTGAG AAATTAGAGA AAGTTTCAGA TTCACGGTTC
 1021 AGGACAGATG GGAAACAGCT GAATATGGCC AACAGGATTA TCTTGTGGTA AGCAGTTCCT
1081 TGCCCCCGCC TCAGCTAGAA CAGATGGAAC AAGCCTTGAA TTATTGGGGC CCCA
//
Primer: Amp_R
Sequence file: RDB14507_A6Led.seq check
>D04073A7_A6Le_1_Amp_R_D10_10_ABI24.ab1
    1 CTCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
   61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
  121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
  181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
  241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
  301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
  361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
  421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
  481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
  541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
  601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
  661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
  721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
  781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
  841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
  901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG
  961 GCCGTTTTTG TGGCCCGACC TGAGTCCAAA ATCCCGATCG TTTTGGGACT CTTTGGTGCA
 1021 CCCCCCTTAG AAGGATGGAT ATGTGATTCT GGTTAGGAGA CCGAGATCTA AACAGTACTC
1081 CTTCCGTCTG AATTTTTTGC CTTTCCGCTT TGGGGAACCG AGC
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14507_A6Lee.seq check
>D04073A7_A6Le_1_SV40pA3element Reverse_E10_13_ABI24.ab1
    1 GTTCGCTGGG GGGGCGGGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
  961 TTTATCGTCG GACCCACTGT GCTGGGCGGG CCCGGCCGCT TTACTTGTAC AGCTCGTCCA
 1021 TGCCCGAGAG TGATCCCAGC GGCGGTCACG ACCTCAGCAG ACCATGTGAA TCGCCGCTTC
1081 TTCGCTGGGA TCTTGGCTTC AACGCTGACT GGAATGCTTC AAGGCTA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]