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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-IRES-GFP_IgG2c (#RDB14506)

Retroviral expression vector of mouse Immunoglobulin G2c (IgG2c).

Clone info. Retroviral expression vector of mouse Immunoglobulin G2c (IgG2c), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg2c cDNA
Depositor|Developer Kitamura, Daisuke |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions:
Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
MTAに書く付加的使用条件：
pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB14506 pMXs-IRES-GFP_IgG2c DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMXs-IRES-GFP_IgG2c was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14506).

Reference section:

Haniuda, K., Fukao, S., Kodama, T., Hasegawa, H., Kitamura, D., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14506_A6Lep1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5'
Sequence file: RDB14506_A6Lea.seq check
>D04073A6_A6Le_1_pBABE5'_D01_10_ABI24.ab1
    1 GTTTTATATG GCCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
   61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
  121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
  181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
  241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
  301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
  361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCACAC
  421 AGGACCTCAC CATGGGATGG AGCTGTATCA TGCTCTTCTT GGCAGCAACA GCTACAGGTG
  481 TCCACTCCCA GGTCCAACTG CAGCAGCCTG GGGCTGAGCT TGTGAAGCCT GGGGCTTCAG
  541 TGAAGCTGTC CTGCAAGGCT TCTGGCTACA CCTTCACCAG CTACTTGATG CACTGGGTGA
  601 AGCAGAGGCC TGGACGAGGC CTTGAGTGGA TTGGAAGGAT TGATCCTAAT AGTGGTGGTA
  661 CTAAGTACAA TGAGAAGTTC AAGAGCAAGG CCACACTGAC TGTAGACAAA CCCTCCAGCA
  721 CAGCCTACAT GCAGCTCAGC AGCCTGACAT CTGAGGACTC TGCGGTCTAT TATTGTGCAA
  781 GATATGATTA CTACGGTAGT AGCTACTTTG ACTACTGGGG CCAAGGCACC ACTCTCACAG
  841 TCTCGAGTGC CAAAACAACA GCCCCATCGG TCTATCCACT GGCCCCTGTG TGTGGAGGTA
  901 CAACTGGCTC CTCGGTGACT CTAGGATGCC TGGGTCAAGG GGTTATTTCC CTGAGCCAGT
  961 GACCTTGACC TGGAACTCTG GGATCCCTGT CCAGTGGTGT GCACACCCTT CCCAGCTCTC
 1021 CCTGCAGTCT GGGCCTCTAC ACCCTCAGCA GCTTCAGTGA CTGTAAACCT CGAACACCTG
 1081 GCCCAGCCAG ACAATCACCT GCAATGTGGC CACCGGCCAG CAGCAACAAG TGGACCAAGA
1141 AAAATTGGAA GCCCAGAGGG TGCCCCATT
//
Primer: IRES-R
Sequence file: RDB14506_A6Leb.seq check
>D04073A6_A6Le_1_IRES-R_F02_17_ABI24.ab1
    1 GCATAGCGGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA GGCTCCCTGC CCAATCATGT TTCTGTAGTC AGGGGAGATC
  121 TTCTGCTTCA GCTCCACCAC AGAGGAGAAG ATCCACTTTA CCTTGAAGAG TGTGACAGAG
  181 GCGCTGTAAC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT TGTCCAGAGC
  241 CCGTCCAGCT CCCCATCCTG GGCCTCAGTA CAGACATCAT CCAGGTCTAG CCCCAGAGAC
  301 CGGGAGATGG TCTTAGTCGT AAGGTGATTG TGCAGACCCT CGTGGACCAC TGAGCAGGCG
  361 AAAAGACTTC CTCTTTCCCA AGTGCTCTTT TGTACTCTGA GCTTGCTGTA CATGAAGTAA
  421 GAACCATCAA AGTCCAGGAC TGTTGCGGTG TTCTTGTAGT TTTGCTCTGT ACGCCCATTG
  481 CTGGTCCAGT CCACAGCAAT TTCGGCAGGT AAGAAGCCTG TGATCATGCA GGTCAGACTG
  541 AACTCTTTCT TAGTCATCTC TTCTGCTGGT GGAGGCAAGA CATATACCTG TGGAGCTCTT
  601 ACTGGCCCTC TGGGTTTTGA GATGGTTTTC TCGATGGGGG ATGGGAGGGC TCTGTTGTTG
  661 ACCTTGCATT TGAACTCCTT GCCACTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG
  721 ACCACCCGGA GAGTACTGTT GTAATCCTCT CTATGGGTTT GTGTCTGAGC TGTGTGTACT
  781 TCCACGTTGT TCACAAACCA GCTGATCTGG ACGTCTGGGT CATCCTCGCT CACATCCACC
  841 ACCACACATG TGACCATGGG GCTCAGGGAG ATCATGAGTA CATCCTTGAT CTTTGGAGGG
  901 AAGATGAAGA CGGATGGTCC ACCCAAGAGG TCTGGAGCTG CGCATGGGGG ACACTCTTTG
  961 AGTGGAGGAC AGGGGTTCTG TGTTATGGGC ACTCTGGCTC AATTTTCTTG TCCACTTTTG
 1021 GTGCTGCTTG CCGGTGGGCC ACATTGGCAG TGATGTCTGC TGGGCCAGTG TCGAAGCTAC
 1081 AGTCACTGAG CTGCTGAGCT GTAAGCCAGA CTGCAGGAAG CTTGAGTGTG CACACCCACT
1141 TGGACACAG
//
Primer: IRES-F
Sequence file: RDB14506_A6Lec.seq check
>D04073A6_A6Le_1_IRES-F_C09_09_ABI24.ab1
    1 GGCAGTCTTA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
  301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
  661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
  721 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
  781 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC GGCCGCCAGC
  841 ACAGTGGTCG ACGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAACG GGGGGAATGA
  901 AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA
  961 AAAATACATA AACTGAGAAT AGAGAAGTTT CAGATCAACG TCAGGAACAG ATGGGAACAG
 1021 CTGATATGGG CCAAACAGGG ATATCTGTGT TAGGCAGATC TGCCCCGGGC TCAGCTAGAC
1081 AGATGGAACA GCTGATTATG GACCAAACCA TGA
//
Primer: Amp_R
Sequence file: RDB14506_A6Led.seq check
>D04073A6_A6Le_1_Amp_R_D09_12_ABI24.ab1
    1 CTTCGTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  901 AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCTGGG
  961 GCCGTTTTTT GTGGCCCGAC CTGAGTCCAA AAATCCCGAT CGTTTTGGAC TTCTTTGGTG
 1021 CACCCCCCCT TAGAGAAGGG ATATTGTGGA TTCTGTTAGA GACCGAGACT AAACCAGTCG
1081 CTTCGATCTG AATTTTGCTT CCGATTGGGG AACCGAC
//
Primer: SV40pA3'element Reverse
Sequence file: RDB14506_A6Lee.seq check
>D04073A6_A6Le_1_SV40pA3element Reverse_E09_15_ABI24.ab1
    1 GTCGTCGGGG GGGCGGAGCC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
   61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
  121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
  181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
  241 CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT AAATACTTAA GAAAAAAAAC
  301 CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  361 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  421 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
  481 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  541 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC AGGTCCTTGG GGCACCCTGG
  601 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC CGCATCTGGG GACCATCTGT
  661 TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC
  721 AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT
  781 GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT GATCTGAACT TCTCTATTCT
  841 CAGTTATGTA TTTTTTCCAT GCCTTGCAAA ATGGCGTTAC TTAAGCTAGC TTGCCAAACC
  901 TACAGGTGGG GGTCTTTTCA TTCCCCCCCC TTTTTTCTGG AGACTAAATA AAAATCTTTT
  961 TAATTTTTAT CGTCGACCCA CTGTGCTGGG CGGGCCGGGC CGCTTTTACT TTGTACAGCT
 1021 CGTCCCATGG CCGAGAGTGA TCCCCAGCCG GGCGGTCACG AACTTCCAGC AGGACCAATG
1081 TGAATCGCGC TTCTCGATTG GGGGATCTTT TGGCCTCCAA TGGGTCCGGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827. [link to RRC of NBRP]