Resource data sheet
Prev.

pMXs-IRES-GFP_IgG2c

Retroviral expression vector of mouse Immunoglobulin G2c (IgG2c).

Catalog number RDB14506
Resource name pMXs-IRES-GFP_IgG2c
Clone info. Retroviral expression vector of mouse Immunoglobulin G2c (IgG2c), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg2c cDNA
Depositor Kitamura, Daisuke |
 
Other clones in our bank mouse Ighg2c (NCBI Gene 404711) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
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RDB14506 pMXs-IRES-GFP_IgG2c DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14506_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' RDB14506_A6Lea.seq
>D04073A6_A6Le_1_pBABE5'_D01_10_ABI24.ab1
    1 GTTTTATATG GCCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
   61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
  121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
  181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
  241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
  301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
  361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCACAC
  421 AGGACCTCAC CATGGGATGG AGCTGTATCA TGCTCTTCTT GGCAGCAACA GCTACAGGTG
  481 TCCACTCCCA GGTCCAACTG CAGCAGCCTG GGGCTGAGCT TGTGAAGCCT GGGGCTTCAG
  541 TGAAGCTGTC CTGCAAGGCT TCTGGCTACA CCTTCACCAG CTACTTGATG CACTGGGTGA
  601 AGCAGAGGCC TGGACGAGGC CTTGAGTGGA TTGGAAGGAT TGATCCTAAT AGTGGTGGTA
  661 CTAAGTACAA TGAGAAGTTC AAGAGCAAGG CCACACTGAC TGTAGACAAA CCCTCCAGCA
  721 CAGCCTACAT GCAGCTCAGC AGCCTGACAT CTGAGGACTC TGCGGTCTAT TATTGTGCAA
  781 GATATGATTA CTACGGTAGT AGCTACTTTG ACTACTGGGG CCAAGGCACC ACTCTCACAG
  841 TCTCGAGTGC CAAAACAACA GCCCCATCGG TCTATCCACT GGCCCCTGTG TGTGGAGGTA
  901 CAACTGGCTC CTCGGTGACT CTAGGATGCC TGGGTCAAGG GGTTATTTCC CTGAGCCAGT
  961 GACCTTGACC TGGAACTCTG GGATCCCTGT CCAGTGGTGT GCACACCCTT CCCAGCTCTC
 1021 CCTGCAGTCT GGGCCTCTAC ACCCTCAGCA GCTTCAGTGA CTGTAAACCT CGAACACCTG
 1081 GCCCAGCCAG ACAATCACCT GCAATGTGGC CACCGGCCAG CAGCAACAAG TGGACCAAGA
1141 AAAATTGGAA GCCCAGAGGG TGCCCCATT
//
Primer: IRES-R RDB14506_A6Leb.seq
>D04073A6_A6Le_1_IRES-R_F02_17_ABI24.ab1
    1 GCATAGCGGG CTTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA GGCTCCCTGC CCAATCATGT TTCTGTAGTC AGGGGAGATC
  121 TTCTGCTTCA GCTCCACCAC AGAGGAGAAG ATCCACTTTA CCTTGAAGAG TGTGACAGAG
  181 GCGCTGTAAC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT TGTCCAGAGC
  241 CCGTCCAGCT CCCCATCCTG GGCCTCAGTA CAGACATCAT CCAGGTCTAG CCCCAGAGAC
  301 CGGGAGATGG TCTTAGTCGT AAGGTGATTG TGCAGACCCT CGTGGACCAC TGAGCAGGCG
  361 AAAAGACTTC CTCTTTCCCA AGTGCTCTTT TGTACTCTGA GCTTGCTGTA CATGAAGTAA
  421 GAACCATCAA AGTCCAGGAC TGTTGCGGTG TTCTTGTAGT TTTGCTCTGT ACGCCCATTG
  481 CTGGTCCAGT CCACAGCAAT TTCGGCAGGT AAGAAGCCTG TGATCATGCA GGTCAGACTG
  541 AACTCTTTCT TAGTCATCTC TTCTGCTGGT GGAGGCAAGA CATATACCTG TGGAGCTCTT
  601 ACTGGCCCTC TGGGTTTTGA GATGGTTTTC TCGATGGGGG ATGGGAGGGC TCTGTTGTTG
  661 ACCTTGCATT TGAACTCCTT GCCACTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG
  721 ACCACCCGGA GAGTACTGTT GTAATCCTCT CTATGGGTTT GTGTCTGAGC TGTGTGTACT
  781 TCCACGTTGT TCACAAACCA GCTGATCTGG ACGTCTGGGT CATCCTCGCT CACATCCACC
  841 ACCACACATG TGACCATGGG GCTCAGGGAG ATCATGAGTA CATCCTTGAT CTTTGGAGGG
  901 AAGATGAAGA CGGATGGTCC ACCCAAGAGG TCTGGAGCTG CGCATGGGGG ACACTCTTTG
  961 AGTGGAGGAC AGGGGTTCTG TGTTATGGGC ACTCTGGCTC AATTTTCTTG TCCACTTTTG
 1021 GTGCTGCTTG CCGGTGGGCC ACATTGGCAG TGATGTCTGC TGGGCCAGTG TCGAAGCTAC
 1081 AGTCACTGAG CTGCTGAGCT GTAAGCCAGA CTGCAGGAAG CTTGAGTGTG CACACCCACT
1141 TGGACACAG
//
Primer: IRES-F RDB14506_A6Lec.seq
>D04073A6_A6Le_1_IRES-F_C09_09_ABI24.ab1
    1 GGCAGTCTTA TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC
   61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA
  121 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA
  181 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA
  241 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC
  301 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA
  361 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA
  421 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT
  481 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA
  541 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT
  601 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA
  661 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC
  721 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC
  781 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG TAAAGCGGCC GGCCGCCAGC
  841 ACAGTGGTCG ACGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAACG GGGGGAATGA
  901 AAGACCCCAC CTGTAGGTTT GGCAAGCTAG CTTAAGTAAC GCCATTTTGC AAGGCATGGA
  961 AAAATACATA AACTGAGAAT AGAGAAGTTT CAGATCAACG TCAGGAACAG ATGGGAACAG
 1021 CTGATATGGG CCAAACAGGG ATATCTGTGT TAGGCAGATC TGCCCCGGGC TCAGCTAGAC
1081 AGATGGAACA GCTGATTATG GACCAAACCA TGA
//
Primer: Amp_R RDB14506_A6Led.seq
>D04073A6_A6Le_1_Amp_R_D09_12_ABI24.ab1
    1 CTTCGTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
  181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
  241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
  301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
  361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
  421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
  481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
  541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
  601 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC
  661 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT
  721 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA
  781 GGTAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT
  841 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG
  901 AACTGACGAG TTCGGAACAC CCGGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCTGGG
  961 GCCGTTTTTT GTGGCCCGAC CTGAGTCCAA AAATCCCGAT CGTTTTGGAC TTCTTTGGTG
 1021 CACCCCCCCT TAGAGAAGGG ATATTGTGGA TTCTGTTAGA GACCGAGACT AAACCAGTCG
1081 CTTCGATCTG AATTTTGCTT CCGATTGGGG AACCGAC
//
Primer: SV40pA3'element Reverse RDB14506_A6Lee.seq
>D04073A6_A6Le_1_SV40pA3element Reverse_E09_15_ABI24.ab1
    1 GTCGTCGGGG GGGCGGAGCC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
   61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
  121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
  181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
  241 CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT AAATACTTAA GAAAAAAAAC
  301 CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG CTGACGGGTA GTCAATCACT
  361 CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG GATGCAACTG CAAGAGGGTT
  421 TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA GGACTGGCGC CCCGAGTGAG
  481 GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG CGCGAACAGA AGCGAGAAGC
  541 GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC AGGTCCTTGG GGCACCCTGG
  601 AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC CGCATCTGGG GACCATCTGT
  661 TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT ATCCTGTTTG GCCCATATTC
  721 AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA CTGCTTACCA CAGATATCCT
  781 GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT GATCTGAACT TCTCTATTCT
  841 CAGTTATGTA TTTTTTCCAT GCCTTGCAAA ATGGCGTTAC TTAAGCTAGC TTGCCAAACC
  901 TACAGGTGGG GGTCTTTTCA TTCCCCCCCC TTTTTTCTGG AGACTAAATA AAAATCTTTT
  961 TAATTTTTAT CGTCGACCCA CTGTGCTGGG CGGGCCGGGC CGCTTTTACT TTGTACAGCT
 1021 CGTCCCATGG CCGAGAGTGA TCCCCAGCCG GGCGGTCACG AACTTCCAGC AGGACCAATG
1081 TGAATCGCGC TTCTCGATTG GGGGATCTTT TGGCCTCCAA TGGGTCCGGG A
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl