Resource data sheet
Prev.

pMXs-IRES-GFP_IgG2b

Retroviral expression vector of mouse Immunoglobulin G2b (IgG2b).

Catalog number RDB14505
Resource name pMXs-IRES-GFP_IgG2b
Clone info. Retroviral expression vector of mouse Immunoglobulin G2b (IgG2b), cloned into the pMXs-IRES-GFP. Refer Fig.1 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Expression was confirmed by the depositor with flow cytometry. Sequence primer for IgH 5term-GCGGTCTATTATTGTGCAAG-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Ighg2b cDNA
Depositor Kitamura, Daisuke |
 
Other clones in our bank mouse Ighg2b (NCBI Gene 16016) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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Material Transfer Agreement (MTA) [Word]
Schedule A (GFP-A) [Link]
Remarks ((Additional form)) GFP-A
Remember that you will be working with samples containing infectious virus.
Please use Schedule A (GFP-A) additionally.
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RDB14505 pMXs-IRES-GFP_IgG2b DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14505_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' RDB14505_A6Lea.seq
>D04073A5_A6Le_1_pBABE5'_C01_07_ABI24.ab1
    1 GTCTTATATG GCCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG
   61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT
  121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC
  181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA
  241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG
  301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC
  361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCACAC
  421 AGGACCTCAC CATGGGATGG AGCTGTATCA TGCTCTTCTT GGCAGCAACA GCTACAGGTG
  481 TCCACTCCCA GGTCCAACTG CAGCAGCCTG GGGCTGAGCT TGTGAAGCCT GGGGCTTCAG
  541 TGAAGCTGTC CTGCAAGGCT TCTGGCTACA CCTTCACCAG CTACTTGATG CACTGGGTGA
  601 AGCAGAGGCC TGGACGAGGC CTTGAGTGGA TTGGAAGGAT TGATCCTAAT AGTGGTGGTA
  661 CTAAGTACAA TGAGAAGTTC AAGAGCAAGG CCACACTGAC TGTAGACAAA CCCTCCAGCA
  721 CAGCCTACAT GCAGCTCAGC AGCCTGACAT CTGAGGACTC TGCGGTCTAT TATTGTGCAA
  781 GATATGATTA CTACGGTAGT AGCTACTTTG ACTACTGGGG CCAAGGCACC ACTCTCACAG
  841 TCTCGACTGC CAAAACAACA CCCCCATCAG TCTATCCACT GGCCCCTGGG TGTGGAGATA
  901 CAACTGGTTC CTCTGTGACT CTGGGGATGC CTGGTCAAGG GCTACTTCCC TGAGTCAGTG
  961 ACTGTGACTT GGAACTCTGG ATCCCTGTCC AGCAGTGTGC ACACCTTCCC AGCTCTCCTG
 1021 CAGTCTGGAC TCTACACTAT GAGCAGCTCA GTGACTGTCC CCTCCAGCAC CTGGCCAAGT
 1081 CAGACCGTCA CCTGCAGCGT TGCTCACCCA GGCAGGCAGC AACCACGGTG GTAACAAAAA
1141 ACTTGAAGCC CAGGCCGGGG CCCCCACAAT CA
//
Primer: IRES-R RDB14505_A6Leb.seq
>D04073A5_A6Le_1_IRES-R_E02_14_ABI24.ab1
    1 GCCATCAGCG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA
   61 ATTTACGTAG CGGCCGCCTA GGCTCCCTGC CCAATCATGT TTCTGTAGTC AGGGGAGATC
  121 TTCTGCTTCA GCTCCACCAC TGAGGAGAAG ATCCACTTTA CCTTGAAGAG TGTGACAGAG
  181 GCGCTGTAGC ACACGCTGAG CAGGAAGAGG CTGATGAAGA TGGTGATGGT CGTCCAGAGC
  241 CCGTCCAGCT CCCCATCCTT GGCCTCAGCA CAGATATCAT CCAGGTCTAG CCCCGGAGAC
  301 CGGGAGATGG TCTTCTTCAG GTAGTAATTT TTCAGACCCT CGTGTCTCAC GTTGCATGAG
  361 AAGGAATCTG TTTTCTCCCA CTTGCTTGTT TTTATATCGA GCTTGCTGTA TATGAAGTAA
  421 GAACCGTCAG AGTCCAGGAC TGGTGCGGTG TCCTTGTAGT TCTCCTCTGT ATGCCCATTG
  481 CTGGTCCACT CCACACTGAT GTCTCCAGGG TTGAAGCCCA CGACCAGGCA AGTGAGACTG
  541 ACATCTTTCC TGGACAACTG CTCTGCTGGT GGCGGCAAGA TGTATACTTG TGGAGCTCTG
  601 ACTAGCCCTT TAATTTTTGA GATGGTTCTC TCGATGGGTG ATGGGAGGTC TTTGTTGTTG
  661 ACCTTGCATT TGAACTCCTT GCCACTCATC CAGTCCTGGT GCTGGATGGG GAGGGCACTG
  721 ACCACCCGGA TAGTACTGTT GTAATCCTCT CTATGGGTTT GTGTCTGAGC TGTGTGTACT
  781 TCCACGTTGT TCACAAACCA GCTGATCCGG ACGTCTGGGT CATCCTCGCT CACATCCACC
  841 ACCACACACG TGACCTTGGG TGTCAGGGAG ATCATGAGTA CATCCTTGAT ATTTGGAGGA
  901 AAGATGAAGA CGGATGGTCC ACCCTCGAGG TTAGGAGCTG GGCATTTGTG ACACTCCCTT
  961 GCATGGAGGA CAGGGGTTGA TTGTTGAAAT GGGCCCGCTG GGCTCAAGTT TTTTGTCCAC
 1021 CGTGGTGCTG CTGGCTGGGT GAGCACGCTG CAGTGACGTC TGACTTGCCA GTGCTTGAAG
 1081 GGACAGTCAC TGAGCTGCTC ATAGGTAAGT TCAGACTGCA GGAAGCCTGG CAGTGTTGCA
1141 CACTTGCCTT GGAACAGCA
//
Primer: IRES-F RDB14505_A6Lec.seq
>D04073A5_A6Le_1_IRES-F_C08_08_ABI24.ab1
    1 GGCAGTGCTT TAAATGTGTT TAGTCGAGGT TAAAAAACGT CTAGGCCCCC CGAACCACGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATG ATAATATGGC CACAACCATG GTGAGCAAGG
  121 GCGAGGAGCT GTTCACCGGG GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG
  181 GCCACAAGTT CAGCGTGTCC GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC
  241 TGAAGTTCAT CTGCACCACC GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC
  301 TGACCTACGG CGTGCAGTGC TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT
  361 TCAAGTCCGC CATGCCCGAA GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG
  421 GCAACTACAA GACCCGCGCC GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG
  481 AGCTGAAGGG CATCGACTTC AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA
  541 ACTACAACAG CCACAACGTC TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA
  601 ACTTCAAGAT CCGCCACAAC ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC
  661 AGAACACCCC CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC
  721 AGTCCGCCCT GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG
  781 TGACCGCCGC CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTAAAGC GGCCGGCCGC
  841 CAGCACAGTG GTCGACGATA AAATAAAAGA TTTTATTTAG TCTCCAGAAA AAGGGGGGAA
  901 TGAAAGACCC CACCTGTAAG TTTGGCAAGC TAGCTTAAGT AACGCCATTT TTGCAAGGCA
  961 TGGAAAAATA CATAACTGAG AAATAGAGAA GTTCAGATCA AAGGTCAGAA CAGATGGAAA
 1021 CAGCTGAATA TGGCCCAAAC AGGATATCTG TGGTAGCAGT TCCTGCCCCC GGCTTCAGGT
1081 CAGAACAGGA TTGGAACAGC CTGAATATTG GCCCAAACCA AGG
//
Primer: Amp_R RDB14505_A6Led.seq
>D04073A5_A6Le_1_Amp_R_D08_11_ABI24.ab1
    1 CTTCCTTTTT TCATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
   61 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT
  121 GCCACCTGCA TAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA AGTAACGCCA
  181 TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAAA AGTTCAGATC AAGGTCAGGA
  241 ACAGATGGAA CAGCTGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC
  301 GGCTCAGGGC CAAGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
  361 AGCAGTTCCT GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCAGCCCT
  421 CAGCAGTTTC TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC
  481 TGTGCCTTAT TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT
  541 CCCCGAGCTC AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATTGAC
  601 TGAGTCGCCC GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG
  661 TCTCGCTGTT CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT
  721 TCATTTGGGG GCTCGTCCGG GATCGGGAGA CCCCTGCCCA GGGACCACCG ACCCACCACC
  781 GGGAGGTAAG CTGGCCAGCA ACTTATCTGT GTCTGTCCGA TTGTCTAGTG TCTATGACTG
  841 ATTTTATGCG CCTGCGTCGG TACTAGTTAG CTAACTAGCT CTGTATCTGG CGGACCCGTG
  901 GTGGAACTGA CGAGTTCGGA ACACCCGGCC GCAACCCTGG GAGACGTCCC AGGGACTTCG
  961 GGGGGCCGTT TTTGTGGCCC GACCTGAAGT CCAAAAATCC CGATCGTTTT GGGACTCTTT
 1021 GGTGCACCCC CCTTTAGAAG AGGGATATGT GATCCTGTAA GAGACGAAGA CTTAAACAGA
1081 TCCCGGCCTC CGTCCTGAAT TTTTGCCATT CCGAATTTTG G
//
Primer: SV40pA3'element Reverse RDB14505_A6Lee.seq
>D04073A5_A6Le_1_SV40pA3element Reverse_E08_14_ABI24.ab1
    1 GTAGGCGGGG GGGCGGACCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
   61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
  121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
  181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
  241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
  301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
  361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
  421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
  481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
  541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
  601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
  661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
  721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
  781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
  841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
  901 CAGGTGGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAA TCTTTTTATT
  961 TTTATCGTCG ACCCACTGTG CTGGGCGGCC CGGCCGCTTT TACTTGTACA GCTCGTTCCA
 1021 TGCCGAGAGG TGATTCCCGG CGGCGGTCAC GACTCCAGCA GACCAATGTG ATCGCGCATT
1081 CTCGGTTGGG GATCTTTTGG CTTCAAGGGA CCGGGAACCT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl